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Alexis Madrid, PhD

Articles by Alexis Madrid, PhD

About Alexis Madrid, PhD

Alexis Madrid holds a PhD in Molecular and Cell Biology at the University of California, Berkeley. She is a Senior Scientist in the Bioscience Department at Biotium.

TECH TIP

App Note: DNase pre-treatment of the EV exterior improves EV RNA yield

Application note comparing affects of on-column DNase treatment and DNase treatment of bead-bound extracellular vesicles during EV RNA extraction.

BY: Alexis Madrid, PhD
Alexis Madrid, PhD
March 18, 2025
TECH TIP

Tech Tip: Tips & Best Practices for Working with RNA

Considerations for Doing RNA Work Working with RNA can be challenging and intimidating compared to working with DNA. While DNA is known to be quite stable and hardy (geneticists have been sequencing genomes that are thousands of years old), the chemical structure of RNA makes it prone to hydrolysis and, therefore, much less stable. And […]

BY: Alexis Madrid, PhD
Alexis Madrid, PhD
February 7, 2025
TECH TIP

Tech Tip: Fluorescent Detection of EVs by Flow Cytometry

Want a printable copy of this tech tip? Fill out the form below to sign up for our e-newsletter and receive a copy.   Extracellular vesicles (EVs) are small, membrane-bound particles secreted from cells and thought to function as cellular messengers, carrying cargo from one cell to another. In biomedical research, EVs and their cargo […]

BY: Alexis Madrid, PhD
Alexis Madrid, PhD
August 16, 2024
TECH TIP

Tech Tip: Isolation and Staining of Extracellular Vesicles

Want a printable copy of this tech tip? Fill out the form below to sign up for our e-newsletter and receive a copy.   Extracellular vesicles (EVs) are small, membrane-bound particles secreted from cells and thought to function as cellular messengers, carrying cargo from one cell to another. In biomedical research, EVs and their cargo […]

BY: Alexis Madrid, PhD
Alexis Madrid, PhD
April 1, 2024
TECH TIP

Protocol: Intracellular Antibody Staining for Flow Cytometry

Intracellular Staining for Flow Cytometry There are many methods for fixation and permeabilization of cells for flow cytometry. Protocols may need to be optimized for different cell types, targets, or applications. This is our basic protocol for intracellular staining of cells in suspension with antibodies for flow cytometry using our Flow Cytometry Fixation/Permeabilization Kit. For […]

BY: Alexis Madrid, PhD
Alexis Madrid, PhD
October 23, 2023
TECH TIP

Tech Tip: Measuring Cell Division in PMBCs by Flow Cytometry

The level of cell proliferation provides insights into relative cell health and is a widely used diagnostic indicator for numerous cancer types. In immunology, measuring cell proliferation is particularly useful for assessing immune responses such as T cell activation. In the past, cell proliferation assays involved radioactive labelling of DNA, but today fluorescent dyes such […]

BY: Alexis Madrid, PhD
Alexis Madrid, PhD
August 18, 2022
TECH TIP

Protocol: Cell Surface Antibody Staining for Flow Cytometry

Extracellular Staining for Flow Cytometry There are many protocols for staining cells for flow cytometry. Protocols may to need be optimized for different cell types, targets, or applications. This is our basic protocol for extracellular staining of cell surface epitopes in suspension cells for flow cytometry. For intracellular staining, see our Protocol: Intracellular Antibody Staining […]

BY: Alexis Madrid, PhD
Alexis Madrid, PhD
August 11, 2020
BLOG POST

Happy anniversary, NucView®! More than a decade of real-time apoptosis detection

A look back at NucView®, the apoptosis tool scientists were waiting for.

BY: Alexis Madrid, PhD
Alexis Madrid, PhD
November 19, 2018
BLOG POST

12 years later: Viability PCR using PMA and PMAxx™ dyes

Let's take a look back on the many uses of viability PCR in basic research, diagnostics and food safety over the years.

BY: Alexis Madrid, PhD
Alexis Madrid, PhD
June 1, 2018
BLOG POST

Switching from agar plates to v-PCR for bacteria viability testing – What you need to know

Important considerations to help you obtain the best possible data for your next v-PCR experiment.

BY: Alexis Madrid, PhD
Alexis Madrid, PhD
March 2, 2018
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