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Do you offer alternatives to Akoya Opal™ tyramides?

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Apoptosis, Necrosis, and Cell Viability KitsCellBrite® & MemBrite® Membrane & Cell Surface StainsCF® DyesDNA & RNA Quantitation KitsDNAzure® Visible DNA Gel StainEvaGreen® Dye and Master MixesEverBrite™ Mounting Medium & CoverGrip™ Coverslip SealantExosome & EV StainingGelRed® and GelGreen® Nucleic Acid Gel StainsGelRed® and GelGreen® TroubleshootingIon Indicators & ChelatorsLuciferase AssaysMicrobiology: Dyes for bacteria and yeastMix-n-Stain™ Antibody Labeling KitsNucView® Caspase-3 Enzyme SubstratesOrganelle & Cytoskeleton StainsOther DNA/RNA Gel StainsPMA and PMAxx™ for viability PCRProduct shipping, storage, shelf life, & solubilityProtein Detection & AnalysisResazurin Cell Viability AssayRNAstorm™ and DNAstorm™ FFPE extraction kitsSecondary AntibodiesTrueBlack® Background ReducersTyramide Signal Amplification (CF® Dye & Other Tyramides)
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See below for our recommended alternatives to Akoya tyramides. Unless noted as a direct replacement, these tyramide dyes are spectrally similar alternatives. They have not been validated as direct replacements for use with Akoya kits or imaging systems.

CF® Dye Alternatives for Akoya Tyramides

Akoya TyramideEx/Em (nm)Biotium TyramideEx/Em (nm)Biotium Cat. No.NotesCF® Dye Features
TSA Coumarin402/443CF®405S404/43192197Much brighter & more
photostable alternative		CF®405S Features
Opal™ Polaris 480450/500CF®430426/49896053Recommended
for abundant targets		CF®430 Features
Opal™520494/525CF®488A490/51592171CF®488A Features
TSA Fluorescein494/517Fluorescein492/51496018Direct replacementSee CF®488A for a brighter &
much more photostable alternative
TSA TMR
TSA Cyanine 3		550/570Cyanine 555555/56596020Direct replacement
for Cyanine 3		See CF®555 & CF®568 for brighter
& more photostable alternatives.
CF®555555/56596021Brighter & more
photostable alternatives		CF®555 Features
CF®568562/58392173CF®568 Features
Opal™570550/570CF®550R551/57796077CF®550R Features
CF®555555/565
96021CF®555 Features
TSA Plus Cyanine 3.5581/596CF®583R586/60996085Brighter & more
photostable alternatives		CF®583R Features
CF®594593/61492174CF®594 Features
Opal™620588/616CF®583R586/60996085CF®583R Features
TSA Cyanine 5648/667CF®640R642/66292175Brighter & more
photostable alternative		CF®640R Features
CF®647650/665 96022Brighter alternativeCF®647 Features
CF®660R663/68292195Less cross-talk with visible red dyes;
bright & extremely photostable		CF®660R Features
TSA Plus Cyanine 5.5
Opal™690		673/692
676/694		CF®680R680/70192196Bright & extremely
photostable		CF®680R Features
Opal™ Polaris 780750/770CF®754745/78696090Recommended
for abundant targets		Unique NIR dye for TSA
TSA BiotinN/ABiotin-XXN/A92176Direct replacement
TSA Plus DNPN/ADNPN/A96019Direct replacement
TSA Plus DIGN/AN/AN/AN/AN/A
Note: The CF® Dyes listed here are spectrally similar alternatives to Opal™ dyes, they have not been validated as direct replacements for use with Akoya's kits or imaging system. Opal is a trademark of Akoya Biosciences.

 


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