Biotium developed TyraMax™ Amplification Dyes as high-performance TSA dyes, offering brighter, more photostable signals than Aluora® and Opal® reagents. They also remain stable in amplification buffer for up to 24 hours, facilitating automated staining workflows. TyraMax™ Dyes are offered as standalone dye solutions, as 3-color or 5-color dye sets plus DAPI counterstain, and in a sampler for custom panel optimization. See below for alternatives to specific Aluora® and Opal® reagents. Learn more about our reagents for tyramide signal amplification (TSA).
TyraMax™ Amplification Dyes
| Dye | Abs/Em (nm) | Laser Line | Detection channel | Dye Features | Size | Catalog No. |
|---|---|---|---|---|---|---|
| TyraMax™ 410/450 | 408/452 | 405 nm | DAPI/ Alexa Fluor® 405 | Unique tyramide color, spectrally similar to Alexa Fluor® 405 | 20 uL | 96134-20UL |
| 100 uL | 96134-100UL | |||||
| TyraMax™ 430/500 | 421/497 | 405 nm | FITC | Brighter than Aluora® 430 and Opal® 480 | 20 uL | 96135-20UL |
| 100 uL | 96135-100UL | |||||
| TyraMax™ 400/550 | 394/547 | 405 nm | FITC | Unique tyramide color, spectrally similar to Pacific Orange® | 20 uL | 96136-20UL |
| 100 uL | 96136-100UL | |||||
| TyraMax™ 490/520 | 497/518 | 488 nm | FITC | Brighter than Opal® 520, replacement for Aluora® 488 | 20 uL | 96137-20UL |
| 100 uL | 96137-100UL | |||||
| TyraMax™ 555/565 | 594/569 | 555 nm or 561 nm | TRITC | Brighter than Opal® 570, replacement for Aluora® 555 | 20 uL | 96138-20UL |
| 100 uL | 96138-100UL | |||||
| TyraMax™ 560/580 | 562/584 | 555 nm or 561 nm | TRITC | Alternative to Aluora® 555, Opal® 570 with superior photostability | 20 uL | 96139-20UL |
| 100 uL | 96139-100UL | |||||
| TyraMax™ 630/650 | 631/650 | 633 nm or 640 nm | Cy®5 | Bright and photostable alternative to Aluora® 647, Opal® 650 | 20 uL | 96140-20UL |
| 100 uL | 96140-100UL | |||||
| TyraMax™ 647/670 | 650/670 | 633 nm or 640 nm | Cy®5 | Brighter than Opal® 650, replacement for Aluora® 647 | 20 uL | 96141-20UL |
| 100 uL | 96141-100UL | |||||
| TyraMax™ 660/680 | 663/683 | 633 nm or 640 nm | Alexa Fluor® 680 | Unique tyramide color, spectrally similar to Alexa Fluor® 660. Brighter and more photostable Opal® 690 when excited at 640 nm | 20 uL | 96142-20UL |
| 100 uL | 96142-100UL | |||||
| TyraMax™ 680/700 | 680/701 | 685 nm (detectable with 640 nm excitation) | Alexa Fluor® 680 | Brighter and more photostable than Opal® 690 when excited at 685 nm | 20 uL | 96143-20UL |
| 100 uL | 96143-100UL | |||||
| TyraMax™ 710/740 | 711/736 | 685 nm | Alexa Fluor® 700 | Brighter and more photostable than Aluora® 700 | 20 uL | 96144-20UL |
| 100 uL | 96144-100UL | |||||
| TyraMax™ 740/770 | 742/768 | 730 nm | Alexa Fluor® 750 | Single-step detection, unlike Opal® 780. Stable in amplification buffer for up to 24 hours, unlike Aluora® 750 | 20 uL | 96145-20UL |
| 100 uL | 96145-100UL |
TyraMax™ 3-Plex Amplification Dye Set with DAPI (Cat. No. 33029)
| Component # | Component Name | Size |
|---|---|---|
| 96137-100UL | TyraMax™ 490/520 Amplification Dye, 100X, 100 uL | For 100 samples using 100 uL staining volume |
| 96139-100UL | TyraMax™ 560/580 Amplification Dye, 100X, 100 uL | |
| 96140-100UL | TyraMax™ 630/650 Amplification Dye, 100X, 100 uL | |
| 99897-50UL | DAPI, 1000X, 50 uL |
TyraMax™ 5-Plex Amplification Dye Set with DAPI (Cat. No. 33030)
| Component # | Component Name | Size |
|---|---|---|
| 96137-100UL | TyraMax™ 490/520 Amplification Dye, 100X, 100 uL | For 100 samples using 100 uL staining volume |
| 96139-100UL | TyraMax™ 560/580 Amplification Dye, 100X, 100 uL | |
| 96140-100UL | TyraMax™ 630/650 Amplification Dye, 100X, 100 uL | |
| 96143-100UL | TyraMax™ 680/700 Amplification Dye, 100X, 100 uL | |
| 96145-100UL | TyraMax™ 740/770 Amplification Dye, 100X, 100 uL | |
| 99897-50UL | DAPI, 1000X, 50 uL |
TyraMax™ Amplification Dye Spectral Sampler (Cat. No. 33031)
| Component # | Component Name | Size |
|---|---|---|
| 96134-20UL | TyraMax™ 410/450 | For 20 samples per vial using 100 uL staining volume |
| 96135-20UL | TyraMax™ 430/500 | |
| 96136-20UL | TyraMax™ 400/550 | |
| 96137-20UL | TyraMax™ 490/520 | |
| 96138-20UL | TyraMax™ 555/565 | |
| 96139-20UL | TyraMax™ 560/580 | |
| 96140-20UL | TyraMax™ 630/650 | |
| 96141-20UL | TyraMax™ 647/670 | |
| 96142-20UL | TyraMax™ 660/680 | |
| 96143-20UL | TyraMax™ 680/700 | |
| 96144-20UL | TyraMax™ 710/740 | |
| 96145-20UL | TyraMax™ 740/770 |
Aluora is a registered trademark of Thermo Fisher Scientific; Opal is a registered trademark of Akoya Biosciences.
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