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Do you offer alternatives to Akoya Opal™ tyramides?

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Biotium developed TyraMax™ Amplification Dyes as high-performance TSA dyes, offering brighter, more photostable signals than Aluora® and Opal® reagents. They also remain stable in amplification buffer for up to 24 hours, facilitating automated staining workflows. TyraMax™ Dyes are offered as standalone dye solutions, as 3-color or 5-color dye sets plus DAPI counterstain, and in a sampler for custom panel optimization. See below for alternatives to specific Aluora® and Opal® reagents. Learn more about our reagents for tyramide signal amplification (TSA).

TyraMax™ Amplification Dyes

DyeAbs/Em (nm)Laser LineDetection channelDye FeaturesSizeCatalog No.
TyraMax™ 410/450408/452405 nmDAPI/ Alexa Fluor® 405Unique tyramide color, spectrally similar to Alexa Fluor® 40520 uL96134-20UL
100 uL96134-100UL
TyraMax™ 430/500421/497405 nmFITCBrighter than Aluora® 430 and Opal® 48020 uL96135-20UL
100 uL96135-100UL
TyraMax™ 400/550394/547405 nmFITCUnique tyramide color, spectrally similar to Pacific Orange®20 uL96136-20UL
100 uL96136-100UL
TyraMax™ 490/520497/518488 nmFITCBrighter than Opal® 520, replacement for Aluora® 48820 uL96137-20UL
100 uL96137-100UL
TyraMax™ 555/565594/569555 nm or 561 nmTRITCBrighter than Opal® 570, replacement for Aluora® 55520 uL96138-20UL
100 uL96138-100UL
TyraMax™ 560/580562/584555 nm or 561 nmTRITCAlternative to Aluora® 555, Opal® 570 with superior photostability20 uL96139-20UL
100 uL96139-100UL
TyraMax™ 630/650631/650633 nm or 640 nmCy®5Bright and photostable alternative to Aluora® 647, Opal® 65020 uL96140-20UL
100 uL96140-100UL
TyraMax™ 647/670650/670633 nm or 640 nmCy®5Brighter than Opal® 650, replacement for Aluora® 64720 uL96141-20UL
100 uL96141-100UL
TyraMax™ 660/680663/683633 nm or 640 nmAlexa Fluor® 680Unique tyramide color, spectrally similar to Alexa Fluor® 660. Brighter and more photostable Opal® 690 when excited at 640 nm20 uL96142-20UL
100 uL96142-100UL
TyraMax™ 680/700680/701685 nm (detectable with 640 nm excitation)Alexa Fluor® 680Brighter and more photostable than Opal® 690 when excited at 685 nm20 uL96143-20UL
100 uL96143-100UL
TyraMax™ 710/740711/736685 nmAlexa Fluor® 700Brighter and more photostable than Aluora® 70020 uL96144-20UL
100 uL96144-100UL
TyraMax™ 740/770742/768730 nmAlexa Fluor® 750Single-step detection, unlike Opal® 780. Stable in amplification buffer for up to 24 hours, unlike Aluora® 75020 uL96145-20UL
100 uL96145-100UL

TyraMax™ 3-Plex Amplification Dye Set with DAPI (Cat. No. 33029)

Component #Component NameSize
96137-100ULTyraMax™ 490/520 Amplification Dye, 100X, 100 uLFor 100 samples using 100 uL staining volume
96139-100ULTyraMax™ 560/580 Amplification Dye, 100X, 100 uL
96140-100ULTyraMax™ 630/650 Amplification Dye, 100X, 100 uL
99897-50ULDAPI, 1000X, 50 uL

TyraMax™ 5-Plex Amplification Dye Set with DAPI (Cat. No. 33030)

Component #Component NameSize
96137-100ULTyraMax™ 490/520 Amplification Dye, 100X, 100 uLFor 100 samples using 100 uL staining volume
96139-100ULTyraMax™ 560/580 Amplification Dye, 100X, 100 uL
96140-100ULTyraMax™ 630/650 Amplification Dye, 100X, 100 uL
96143-100ULTyraMax™ 680/700 Amplification Dye, 100X, 100 uL
96145-100ULTyraMax™ 740/770 Amplification Dye, 100X, 100 uL
99897-50ULDAPI, 1000X, 50 uL

TyraMax™ Amplification Dye Spectral Sampler (Cat. No. 33031)

Component #Component NameSize
96134-20ULTyraMax™ 410/450For 20 samples per vial using 100 uL staining volume
96135-20ULTyraMax™ 430/500
96136-20ULTyraMax™ 400/550
96137-20ULTyraMax™ 490/520
96138-20ULTyraMax™ 555/565
96139-20ULTyraMax™ 560/580
96140-20ULTyraMax™ 630/650
96141-20ULTyraMax™ 647/670
96142-20ULTyraMax™ 660/680
96143-20ULTyraMax™ 680/700
96144-20ULTyraMax™ 710/740
96145-20ULTyraMax™ 740/770

Aluora is a registered trademark of Thermo Fisher Scientific; Opal is a registered trademark of Akoya Biosciences.


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