Calcium Indicators & Chelators

Membrane-impermeant calcium indicator that can be loaded into cells via microinjection or scrape loading.

Membrane-permeant form of Rhod-2 calcium indicator that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, the mild detergent Pluronic F-127 is often used as a dispersing agent to facilitate dye loading.

Membrane-impermeant calcium indictor that can be loaded into cells via microinjection or scrape loading.

Membrane-impermeant calcium indicator that can be loaded into cells via microinjection or scrape loading.

Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, the mild detergent Pluronic F-127 (catalog no. 59004) is often used as a dispersing agent to facilitate loading.

Fluo-3, pentasodium is membrane-impermeant, but can be loaded into cells via microinjection or scrape loading.

Fluo-3, pentapotassium is membrane-impermeant, but can be loaded into cells via microinjection or scrape loading.

Fluo-3, pentaammonium is membrane-impermeant, but can be loaded into cells via microinjection or scrape loading.

BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium.

BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium.

A-23187, also known as Calcimycin or Calcium Ionophore III, is a calcium ionophore that rapidly equilibrates intracellular and extracellular calcium concentrations. It is commonly used for in situ calibrations of fluorescent calcium indicators.

EDC (or EDAC) (1-(3-Dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride)) has recently been found to be very useful for fixing in situ chelators, including the fluorescent ion indicators for histological studies following physiological experiments. EDC is also a widely used reagent to activate carboxy groups for amine coupling.

Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.

This kit provides buffers for generating a range of accurate calcium concentrations for the calibration of fluorescent Ca²⁺ indicators.

BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium.

BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium.