Enzyme Substrates
Biotium offers substrates for many common enzymes, including luminescent substrates for Firefly and Renilla Luciferases, luciferin and coelenterazine; fluorometric NucView® caspase-3 substrates; various protease substrates; and other enzyme substrates.
Dual Apoptosis Assay with NucView® 488 Caspase-3 Substrate & Annexin V
30030, 30067, - 30073View allHide
These kits provides two apoptosis markers, our novel NucView® 488 Caspase-3 Substrate and Annexin V conjugate for detecting caspase-3 activation and phosphatidylserine (PS) translocation.
NucView® 488 & MitoView™ 633 Apoptosis Assay Kit
No wash, homogeneous assay for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using fluorescence microscopy, real-time imaging, or flow cytometry.
NucView® 488 & RedDot™ 2 Apoptosis & Necrosis Kit
Homogeneous, no wash assay for detection of caspase-3/7 activity with green fluorescence and dead cell staining with far-red fluorescence, for fluorescence microscopy or flow cytometry.
NucView® 488 Caspase-3 Assay Kit for Live Cells
30029, - 30029-TView allHide
Green fluorescent caspase-3/7 substrate for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or real-time live cell imaging.
NucView® Caspase-3 Enzyme Substrates
10405-T, 10405, 10407-T, 10407, 10402-T, 10402, 10403-T, 10403, 10406-T, 10406, 10408-T, - 10408View allHide
Fluorescent caspase-3/7 substrates for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or live cell imaging.
Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit
30009-1, 30009-2, - 30009-3View allHide
A fluorometric endpoint assay kit for detection of caspase-3 activity in cells by high throughput screening (HTS).
D-Luciferin, Free Acid
10100, 10100-1, - 10100-2View allHide
D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.
D-Luciferin, Potassium Salt
10101, 10101-1, - 10101-2View allHide
D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.
D-Luciferin, Sodium Salt
10102, 10102-1, - 10102-2View allHide
D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.
DAB Substrate Kit
DAB reacts with HRP in the presence of peroxide to yield an insoluble brown-colored product at locations where peroxidase-conjugated antibodies are bound to samples.
MTT Cell Viability Assay Kit
A simple, colorimetric assay for determining live cell numbers by absorbance on a microplate reader.
Steady-ATP™ HTS Viability Assay Kit
30138-1, 30138-2, - 30138-3View allHide
A luciferase-based ATP cell viability assay offering high sensitivity, wide dynamic range, and long-lasting signal for high-throughput luminescence detection in microplates.
XTT Cell Viability Assay Kit
Ac-DEVD-R110
A fluorogenic substrate for caspase-3 that can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader.
RNase
A 10 mg/mL RNase solution, DNase-free. Useful for removing RNA from plasmid or genomic DNA preparation, recombinant protein preparations, or for use in ribonuclease protection assays.
RNaseReveal™ Activity Assay Kit
Fluorescent assay for sensitive detection of RNase activity in liquid samples. Ideal for quality control workflows or detecting RNase contamination.