Other Enzyme Substrates
See below for substrates for various enzymes such as peroxidases, phosphatases, β-galactosidase, β-glucuronidase, and others.
DAB Substrate Kit
DAB (3,3′-diaminobenzidine) reacts with HRP in the presence of peroxide to yield an insoluble brown-colored product at locations where peroxidase-conjugated antibodies are bound to samples.
DAB (3,3′-diaminobenzidine) reacts with HRP in the presence of peroxide to yield an insoluble brown-colored product at locations where peroxidase-conjugated antibodies are bound to samples.
MTT Cell Viability Assay Kit
MTT Cell Viability Assay Kit provides a simple method for determining live cell numbers using a standard colorimetric plate readers. Determination of live cell numbers is often used to assess the rate of cell proliferation and cytotoxicity caused by drugs and cytotoxic agents.
MTT Cell Viability Assay Kit provides a simple method for determining live cell numbers using a standard colorimetric plate readers. Determination of live cell numbers is often used to assess the rate of cell proliferation and cytotoxicity caused by drugs and cytotoxic agents.
XTT Cell Viability Assay Kit
XTT Cell Viability Assay Kit provides a simple method for determining live cell numbers using standard photometric microplate readers. Determination of live cell numbers is often used to assess the rate of cell proliferation and to screen cytotoxic agents.
XTT Cell Viability Assay Kit provides a simple method for determining live cell numbers using standard photometric microplate readers. Determination of live cell numbers is often used to assess the rate of cell proliferation and to screen cytotoxic agents.
10-Acetyl-3,7-dihydroxyphenoxazine (Amplex® Red)
10-Acetyl-3,7-dihydroxyphenoxazine (Amplex® Red), is regarded as the best fluorogenic substrate for peroxidase and H2O2 because it is highly sensitive, specific and stable.
10-Acetyl-3,7-dihydroxyphenoxazine (Amplex® Red), is regarded as the best fluorogenic substrate for peroxidase and H2O2 because it is highly sensitive, specific and stable.
ABTS
ABTS (2,2′ -Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid), diammonium salt) is a very sensitive chromogenic peroxidase substrate widely used for ELISA applications. The substrate yields a water-soluble green colored product.
ABTS (2,2′ -Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid), diammonium salt) is a very sensitive chromogenic peroxidase substrate widely used for ELISA applications. The substrate yields a water-soluble green colored product.
BCIP Pink
BCIP Pink (6-Chloro-3-indoxyl phosphate, p-toluidine salt) is a derivative of BCIP. While BCIP yields a blue precipitating product, BCIP Pink produces a pink colored (λmax 540 nm) precipitate.
BCIP Pink (6-Chloro-3-indoxyl phosphate, p-toluidine salt) is a derivative of BCIP. While BCIP yields a blue precipitating product, BCIP Pink produces a pink colored (λmax 540 nm) precipitate.
BCIP Pink/NBT Kit
This kit contains 100 mg each of BCIP Pink (10006) and the precipitation inducer NBT (10008).
This kit contains 100 mg each of BCIP Pink (10006) and the precipitation inducer NBT (10008).
BCIP Red
BCIP Red (5-Bromo-6-chloro-3-indoxyl phosphate, p-toluidine salt ) is a derivative of BCIP. While BCIP yields a blue precipitating product, BCIP Red produces a red (λmax 565 nm) colored precipitate.
BCIP Red (5-Bromo-6-chloro-3-indoxyl phosphate, p-toluidine salt ) is a derivative of BCIP. While BCIP yields a blue precipitating product, BCIP Red produces a red (λmax 565 nm) colored precipitate.
BCIP Red/NBT Kit
This kit contains 100 mg each of BCIP Red (10004) and the oxidant NBT (10008).
This kit contains 100 mg each of BCIP Red (10004) and the oxidant NBT (10008).
BCIP, Na
BCIP (5-Bromo-4-chloro-3-indoxyl phosphate, disodium salt) is the most widely used chromogenic phosphatase substrate, which forms a dark blue (λmax 615 nm) precipitate on enzymatic hydrolysis. It is often used with the oxidant NBT (nitro blue tetrazolium chloride catalog no. 10008), which facilitates the precipitation.
BCIP (5-Bromo-4-chloro-3-indoxyl phosphate, disodium salt) is the most widely used chromogenic phosphatase substrate, which forms a dark blue (λmax 615 nm) precipitate on enzymatic hydrolysis. It is often used with the oxidant NBT (nitro blue tetrazolium chloride catalog no. 10008), which facilitates the precipitation.
BCIP, Toluidine
BCIP, toluidine (5-Bromo-4-chloro-3-indoxyl phosphate, p-toluidine salt) is similar to BCIP/Na (10001).
BCIP, toluidine (5-Bromo-4-chloro-3-indoxyl phosphate, p-toluidine salt) is similar to BCIP/Na (10001).
BCIP/NBT Kit
This kit contains 100 mg each of BCIP sodium salt (10001) and the oxidant NBT (Nitro blue tetrazolium, 10008).
This kit contains 100 mg each of BCIP sodium salt (10001) and the oxidant NBT (Nitro blue tetrazolium, 10008).
Chromogenic β-Galactosidase Substrate Sampler
For researchers wishing to investigate the suitability of our various chromogenic β-galactosidase substrates, we offer this sampler kit, which contains 5 mg each of the following five different substrates: X-Gal (10011), Red-β-D-Gal (10012), Rose-β-D-Gal (10013), Purple-β-D-Gal (10014) and Green-β-D-Gal (10015).
For researchers wishing to investigate the suitability of our various chromogenic β-galactosidase substrates, we offer this sampler kit, which contains 5 mg each of the following five different substrates: X-Gal (10011), Red-β-D-Gal (10012), Rose-β-D-Gal (10013), Purple-β-D-Gal (10014) and Green-β-D-Gal (10015).
Chromogenic β-Glucuronidase Substrate Sampler
For researchers wishing to investigate the suitability of our various chromogenic β-glucuronidase substrates, we offer this sampler kit, which contains 5 mg each of the following three different substrates: X-GlcU, CHA, Red-β-D-GlcU, CHA, and Rose-β-D-GlcU, CHA.
For researchers wishing to investigate the suitability of our various chromogenic β-glucuronidase substrates, we offer this sampler kit, which contains 5 mg each of the following three different substrates: X-GlcU, CHA, Red-β-D-GlcU, CHA, and Rose-β-D-GlcU, CHA.
Chromogenic Phosphatase Substrate Sampler
The sampler kit contains 10 mg each of BCIP Na (10001), BCIP Pink (10006), BCIP Red (10004) and 250 mg of NBT (10008). NBT forms a co-precipitant and enhances the BCIP reaction.
The sampler kit contains 10 mg each of BCIP Na (10001), BCIP Pink (10006), BCIP Red (10004) and 250 mg of NBT (10008). NBT forms a co-precipitant and enhances the BCIP reaction.
CTC (5-Cyano-2,3-ditolyl tetrazolium chloride)
5-Cyano-2,3-ditolyl tetrazolium chloride (CTC) has been used to evaluate the respiratory activity of many bacterial populations as well as populations undergoing drug efficacy evaluations. Healthy cells respiring via the electron transport chain will absorb and reduce CTC into an insoluble, red fluorescent formazan product.
5-Cyano-2,3-ditolyl tetrazolium chloride (CTC) has been used to evaluate the respiratory activity of many bacterial populations as well as populations undergoing drug efficacy evaluations. Healthy cells respiring via the electron transport chain will absorb and reduce CTC into an insoluble, red fluorescent formazan product.