Enzyme Substrates

A luciferase-based ATP cell viability assay offering high sensitivity, wide dynamic range, and long-lasting signal for high-throughput luminescence detection in microplates.

A 10 mg/mL RNase solution, DNase-free. Useful for removing RNA from plasmid or genomic DNA preparation, recombinant protein preparations, or for use in ribonuclease protection assays.

Fluorescent assay for sensitive detection of RNase activity in liquid samples. Ideal for detecting RNase contamination in quality control workflows.

TEV protease kit that may be used for cleaving proteins or peptides containing the TEV protease recognition sequence E-N-L-Y-F-Q↓(S/G/A/M/C/H).

A functionalized version of NAD containing an amino group that permits it to be fixable in a gel matrix. This property makes NAD-hexylamine useful for constructing wearable biosensors that comprise a dehydrogenase enzyme such as diaphorase.

Cytotoxic agent that binds and inhibits dihydrofolate reductase. Useful for studying antimetabolite resistance, spontaneous gene amplification, and drug binding. Available conjugated to fluorescein and CF® Dyes.

A fluorogenic substrate for caspase-3 that can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader.

Fluorescent caspase-3/7 substrates for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or live cell imaging.

Coelenterazine and its analogs are luminescent enzyme substrates for apoaequorin and Renilla luciferase.

5-Cyano-2,3-ditolyl tetrazolium chloride (CTC) has been used to evaluate the respiratory activity of many bacterial populations as well as populations undergoing drug efficacy evaluations.  Healthy cells respiring via the electron transport chain will absorb and reduce CTC into an insoluble, red fluorescent formazan product.

DAB reacts with HRP in the presence of peroxide to yield an insoluble brown-colored product at locations where peroxidase-conjugated antibodies are bound to samples.

Homogeneous, no wash assay for detection of caspase-3/7 activity with green fluorescence and dead cell staining with far-red fluorescence, for fluorescence microscopy or flow cytometry.

Z-AA-R110-PEG (Rhodamine 110-PEG, N-CBZ-L-alanyl-L-alanine amide) is a substrate for elastate.

BZiPAR (Rhodamine 110, bis-(N-CBZ-L-isoleucyl-L-prolyl-L-arginine amide), dihydrochloride) is a substrate for trypsin (1,2). The substrate has been reported to enter live cells and be hydrolyzed by lysosomal proteases (3).

Z-FR-R110 (Rhodamine 110, bis-(N-CBZ-L-phenylalanyl-L-arginine amide), dihydrochloride) is a substrate for the cysteine proteases cathepsins B and L. The substrate has been used in flow cytometry studies of human monocytes and rat macrophages.

Ac-DEVD-AMC is a fluorogenic substrate for caspase 3 and can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader. For real-time detection of caspase-3 activity in intact cells, see our novel NucView®488 Caspase-3 Assay Kit.