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Enzyme Substrates

Biotium offers substrates for many common enzymes, including luminescent substrates for Firefly and Renilla Luciferases, luciferin and coelenterazine; fluorometric NucView® caspase-3 substrates; various protease substrates; and other enzyme substrates.

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    Dual Apoptosis Assay with NucView® 488 Caspase-3 Substrate & Annexin V

    From: $362 Sizes: 50 assaysCatalog #: 30030, 30067, - 30073View allHide

    These kits provides two apoptosis markers, our novel NucView® 488 Caspase-3 Substrate and Annexin V conjugate for detecting caspase-3 activation and phosphatidylserine (PS) translocation.

    Conjugation
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    50 assays
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    These kits provides two apoptosis markers, our novel NucView® 488 Caspase-3 Substrate and Annexin V conjugate for detecting caspase-3 activation and phosphatidylserine (PS) translocation.

     

    NucView® 488 & MitoView™ 633 Apoptosis Assay Kit

    From: $351 Sizes: 100 assaysCatalog #: 30062

    No wash, homogeneous assay for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using fluorescence microscopy, real-time imaging, or flow cytometry.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    100 assays
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    No wash, homogeneous assay for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using fluorescence microscopy, real-time imaging, or flow cytometry.

     

    NucView® 488 & RedDot™ 2 Apoptosis & Necrosis Kit

    From: $308 Sizes: 100 assaysCatalog #: 30072

    Homogeneous, no wash assay for detection of caspase-3/7 activity with green fluorescence and dead cell staining with far-red fluorescence, for fluorescence microscopy or flow cytometry.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    100 assays
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    Homogeneous, no wash assay for detection of caspase-3/7 activity with green fluorescence and dead cell staining with far-red fluorescence, for fluorescence microscopy or flow cytometry.

     

    NucView® 488 Caspase-3 Assay Kit for Live Cells

    From: $98 Sizes: Trial size (25 assays), 100 assaysCatalog #: 30029, - 30029-TView allHide

    Green fluorescent caspase-3/7 substrate for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or real-time live cell imaging.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    Trial size (25 assays)
    100 assays
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    Green fluorescent caspase-3/7 substrate for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or real-time live cell imaging.

     

    NucView® Caspase-3 Enzyme Substrates

    From: $67 Sizes: 10 uL (1 mM in DMSO), 100 uL (1 mM in DMSO), 10 uL (1 mM in PBS), 100 uL (1 mM in PBS)Catalog #: 10405-T, 10405, 10407-T, 10407, 10402-T, 10402, 10403-T, 10403, 10406-T, 10406, 10408-T, - 10408View allHide

    Fluorescent caspase-3/7 substrates for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or live cell imaging.

    Dye
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
    Size
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    10 uL (1 mM in DMSO)
    100 uL (1 mM in DMSO)
    10 uL (1 mM in PBS)
    100 uL (1 mM in PBS)
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    Fluorescent caspase-3/7 substrates for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or live cell imaging.

     

    Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit

    From: $143 Sizes: 25 assays, 100 assaysCatalog #: 30008-1, - 30008-2View allHide

    A fluorometric and colorimetric endpoint assay kit containing a DEVD substrate for detection of caspase-3 activity in cells.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    25 assays
    100 assays
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    A fluorometric and colorimetric endpoint assay kit containing a DEVD substrate for detection of caspase-3 activity in cells.

     

    Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit

    From: $47 Sizes: 10 assays, 100 assays, 1,000 assaysCatalog #: 30009-1, 30009-2, - 30009-3View allHide

    A fluorometric endpoint assay kit for detection of caspase-3 activity in cells by high throughput screening (HTS).

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    10 assays
    100 assays
    1,000 assays
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    A fluorometric endpoint assay kit for detection of caspase-3 activity in cells by high throughput screening (HTS).

     

    Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit

    From: $143 Sizes: 25 assays, 100 assaysCatalog #: 30011-1, - 30011-2View allHide

    A fluorometric and colorimetric endpoint assay kit containing a IETD substrate for detection of caspase-8 activity in cells.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    25 assays
    100 assays
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    A fluorometric and colorimetric endpoint assay kit containing a IETD substrate for detection of caspase-8 activity in cells.

     

    Caspase-8 IETD-R110 Fluorometric HTS Assay Kit

    From: $47 Sizes: 10 assays, 100 assays, 1,000 assaysCatalog #: 30012-1, 30012-2, - 30012-3View allHide

    A fluorometric endpoint assay kit for detection of caspase-8 activity in cells by high throughput screening (HTS).

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    Qty
    10 assays
    100 assays
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    A fluorometric endpoint assay kit for detection of caspase-8 activity in cells by high throughput screening (HTS).

     

    D-Luciferin, Free Acid

    Sizes: 10 mg, 50 mg, 1 gCatalog #: 10100, 10100-1, - 10100-2View allHide

    D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    10 mg
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    1 g
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    D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.

     

    D-Luciferin, Potassium Salt

    Sizes: 10 mg, 50 mg, 1 gCatalog #: 10101, 10101-1, - 10101-2View allHide

    D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    10 mg
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    1 g
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    D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.

     

    D-Luciferin, Sodium Salt

    Sizes: 10 mg, 50 mg, 1 gCatalog #: 10102, 10102-1, - 10102-2View allHide

    D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    10 mg
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    1 g
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    D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.

     

    DAB Substrate Kit

    From: $87 Sizes: 1 kitCatalog #: 30015

    DAB reacts with HRP in the presence of peroxide to yield an insoluble brown-colored product at locations where peroxidase-conjugated antibodies are bound to samples.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    1 kit
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    DAB reacts with HRP in the presence of peroxide to yield an insoluble brown-colored product at locations where peroxidase-conjugated antibodies are bound to samples.

     

    MTT Cell Viability Assay Kit

    From: $143 Sizes: 1,000 assaysCatalog #: 30006
    A simple, colorimetric assay for determining live cell numbers by absorbance on a microplate reader.
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
    Size
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    1,000 assays
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    A simple, colorimetric assay for determining live cell numbers by absorbance on a microplate reader.
     

    XTT Cell Viability Assay Kit

    From: $173 Sizes: 1,000 assaysCatalog #: 30007
    A simple, colorimetric assay for determining live cell numbers by absorbance on a microplate reader.
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
    Size
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    Qty
    1,000 assays
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    A simple, colorimetric assay for determining live cell numbers by absorbance on a microplate reader.
     

    Ac-DEVD-R110

    From: $95 Sizes: 1 mgCatalog #: 10226

    A fluorogenic substrate for caspase-3 that can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
    Size
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    Qty
    1 mg
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    A fluorogenic substrate for caspase-3 that can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader.