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Enzyme Substrates

Biotium offers substrates for many common enzymes, including luminescent substrates for Firefly and Renilla Luciferases, luciferin and coelenterazine; fluorometric NucView® caspase-3 substrates; various protease substrates; and other enzyme substrates.

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    Dual Apoptosis Assay with NucView® 488 Caspase-3 Substrate & Annexin V

    From: $351 Sizes: 50 assaysCatalog #: 30030, 30067, - 30073View allHide

    These kits provides two apoptosis markers, our novel NucView® 488 Caspase-3 Substrate and Annexin V conjugated with your choice of red or far-red dye. The kit is used for studying two important apoptosis events, caspase-3 activation and phosphatidylserine (PS) translocation.

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    These kits provides two apoptosis markers, our novel NucView® 488 Caspase-3 Substrate and Annexin V conjugated with your choice of red or far-red dye. The kit is used for studying two important apoptosis events, caspase-3 activation and phosphatidylserine (PS) translocation.

     

    NucView® 405 Caspase-3 Substrate, 1 mM in DMSO

    From: $65 Sizes: Trial size (10 uL), 100 uLCatalog #: 10405, - 10405-TView allHide

    NucView® 405 Caspase-3 Substrate, 1 mM in DMSO, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

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    NucView® 405 Caspase-3 Substrate, 1 mM in DMSO, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

     

    NucView® 405 Caspase-3 Substrate, 1 mM in PBS

    Sizes: Trial size (10 uL), 100 uLCatalog #: 10407-T, - 10407View allHide

    NucView® 405 Caspase-3 Substrate, 1 mM in PBS, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

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    NucView® 405 Caspase-3 Substrate, 1 mM in PBS, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

     

    NucView® 488 and MitoView™ 633 Apoptosis Assay Kit

    From: $351 Sizes: 100 assaysCatalog #: 30062

    This kit contains the NucView®488 Caspase-3 Substrate and the MitoView™633 mitochondrial dye. The kit provides a convenient tool for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using either fluorescence microscopy or flow cytometry.

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    100 assays
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    This kit contains the NucView®488 Caspase-3 Substrate and the MitoView™633 mitochondrial dye. The kit provides a convenient tool for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using either fluorescence microscopy or flow cytometry.

     

    NucView® 488 and RedDot™ 2 Apoptosis and Necrosis Kit

    From: $308 Sizes: 100 assaysCatalog #: 30072

    NucView®488 and RedDot™2 Apoptosis/Necrosis Assay Kit contains NucView®488 Caspase-3 Substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity.

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    100 assays
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    NucView®488 and RedDot™2 Apoptosis/Necrosis Assay Kit contains NucView®488 Caspase-3 Substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity.

     

    NucView® 488 Caspase-3 Assay Kit for Live Cells

    From: $95 Sizes: Trial size (25 assays), 100 assaysCatalog #: 30029, - 30029-TView allHide

    NucView® 488 Caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time.

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    NucView® 488 Caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time.

     

    NucView® 488 Caspase-3 Enzyme Substrate, 1 mM in DMSO

    From: $65 Sizes: 10 uL, 100 uLCatalog #: 10402, - 10402-TView allHide

    NucView® 488 Caspase-3 Substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3/7 activity within live cells in real time.

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    NucView® 488 Caspase-3 Substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3/7 activity within live cells in real time.

     

    NucView® 488 Caspase-3 Enzyme Substrate, 1 mM in PBS

    From: $65 Sizes: 10 uL, 100 uLCatalog #: 10403, - 10403-TView allHide

    NucView® 488 Caspase-3 Substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3/7 activity within live cells in real time.

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    NucView® 488 Caspase-3 Substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3/7 activity within live cells in real time.

     

    NucView® 530 Caspase-3 Substrate, 1 mM in DMSO

    From: $65 Sizes: Trial size (10 uL), 100 uLCatalog #: 10406, - 10406-TView allHide

    NucView® 530 Caspase-3 Substrate, 1 mM in DMSO, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

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    NucView® 530 Caspase-3 Substrate, 1 mM in DMSO, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

     

    NucView® 530 Caspase-3 Substrate, 1 mM in PBS

    Sizes: Trial size (10 uL), 100 uLCatalog #: 10408, - 10408-TView allHide

    NucView® 530 Caspase-3 Substrate, 1 mM in PBS, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

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    NucView® 530 Caspase-3 Substrate, 1 mM in PBS, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

     

    Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit

    From: $143 Sizes: 25 assays, 100 assaysCatalog #: 30008-1, - 30008-2View allHide

    Caspase-3 is an active cell-death protease involved in the execution phase of apoptosis, during which cells undergo morphological changes such as DNA fragmentation, chromatin condensation and apoptotic body formation.

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    Caspase-3 is an active cell-death protease involved in the execution phase of apoptosis, during which cells undergo morphological changes such as DNA fragmentation, chromatin condensation and apoptotic body formation.

     

    Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit

    From: $47 Sizes: 10 assays, 100 assays, 1,000 assaysCatalog #: 30009-1, 30009-2, - 30009-3View allHide

    Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit is specifically designed for HTS-based assays. The kit provides a homogenous assay system for fast and highly sensitive detection of caspase-3 activity by fluorescence in enzymatic reaction or mammalian cells.

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    Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit is specifically designed for HTS-based assays. The kit provides a homogenous assay system for fast and highly sensitive detection of caspase-3 activity by fluorescence in enzymatic reaction or mammalian cells.

     

    Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit

    From: $143 Sizes: 25 assays, 100 assaysCatalog #: 30011-1, - 30011-2View allHide

    Caspase-8 is the most upstream caspase in the CD95/Fas apoptotic pathway and is activated by the signaling pathways for CD95/Fas and TNF. Caspase-8 Fluorometric and Colorimetric Assay Kit provides a simple assay system for fast and sensitive detection of caspase-8 activity either by fluorescence or absorbance.

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    Caspase-8 is the most upstream caspase in the CD95/Fas apoptotic pathway and is activated by the signaling pathways for CD95/Fas and TNF. Caspase-8 Fluorometric and Colorimetric Assay Kit provides a simple assay system for fast and sensitive detection of caspase-8 activity either by fluorescence or absorbance.

     

    Caspase-8 IETD-R110 Fluorometric HTS Assay Kit

    From: $47 Sizes: 10 assays, 100 assays, 1,000 assaysCatalog #: 30012-1, 30012-2, - 30012-3View allHide

    Caspase-8 IETD-R110 Fluorometric HTS Assay Kit is specifically designed for HTS-based assays. The kit provides a homogenous assay system for fast and highly sensitive detection of caspase-8 activity by fluorescence in enzymatic reaction or mammalian cells.

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    Caspase-8 IETD-R110 Fluorometric HTS Assay Kit is specifically designed for HTS-based assays. The kit provides a homogenous assay system for fast and highly sensitive detection of caspase-8 activity by fluorescence in enzymatic reaction or mammalian cells.

     

    D-Luciferin, Free Acid

    From: $58 Sizes: 10 mg, 50 mg, 1 gCatalog #: 10100, 10100-1, - 10100-2View allHide

    D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.

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    D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.

     

    D-Luciferin, Potassium Salt

    From: $58 Sizes: 10 mg, 50 mg, 1 gCatalog #: 10101, 10101-1, - 10101-2View allHide

    D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.

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    D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.