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Enzyme Substrates

Biotium offers substrates for many common enzymes, including luminescent substrates for Firefly and Renilla Luciferases, luciferin and coelenterazine; fluorometric NucView® caspase-3 substrates; various protease substrates; and other enzyme substrates.

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    Dual Apoptosis Assay with NucView® 488 Caspase-3 Substrate & Annexin V

    From: $386 Sizes: 50 assaysCatalog #:
    30030
    , 30067, - 30073View allHide

    These kits provides two apoptosis markers, our novel NucView® 488 Caspase-3 Substrate and Annexin V conjugate for detecting caspase-3 activation and phosphatidylserine (PS) translocation.

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    50 assays
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    These kits provides two apoptosis markers, our novel NucView® 488 Caspase-3 Substrate and Annexin V conjugate for detecting caspase-3 activation and phosphatidylserine (PS) translocation.

     
    Product

    NucView® 488 & MitoView™ 633 Apoptosis Assay Kit

    From: $386 Sizes: 100 assaysCatalog #: 30062

    No wash, homogeneous assay for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using fluorescence microscopy, real-time imaging, or flow cytometry.

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    100 assays
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    No wash, homogeneous assay for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using fluorescence microscopy, real-time imaging, or flow cytometry.

     
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    NucView® 488 & RedDot™ 2 Apoptosis & Necrosis Kit

    From: $338 Sizes: 100 assaysCatalog #: 30072

    Homogeneous, no wash assay for detection of caspase-3/7 activity with green fluorescence and dead cell staining with far-red fluorescence, for fluorescence microscopy or flow cytometry.

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    100 assays
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    Homogeneous, no wash assay for detection of caspase-3/7 activity with green fluorescence and dead cell staining with far-red fluorescence, for fluorescence microscopy or flow cytometry.

     
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    NucView® 488 Caspase-3 Assay Kit for Live Cells

    From: $103 Sizes: Trial size (25 assays), 100 assaysCatalog #:
    30029
    , - 30029-TView allHide

    Green fluorescent caspase-3/7 substrate for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or real-time live cell imaging.

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    Trial size (25 assays)
    100 assays
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    Green fluorescent caspase-3/7 substrate for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or real-time live cell imaging.

     
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    NucView® Caspase-3 Enzyme Substrates

    From: $71 Sizes: 10 uL (1 mM in DMSO), 100 uL (1 mM in DMSO), 10 uL (1 mM in PBS), 100 uL (1 mM in PBS)Catalog #:
    10405-T
    , 10405, 10407-T, 10407, 10402-T, 10402, 10403-T, 10403, 10406-T, 10406, 10408-T, - 10408View allHide

    Fluorescent caspase-3/7 substrates for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or live cell imaging.

    Dye
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    10 uL (1 mM in DMSO)
    100 uL (1 mM in DMSO)
    10 uL (1 mM in PBS)
    100 uL (1 mM in PBS)
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    Fluorescent caspase-3/7 substrates for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or live cell imaging.

     
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    Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit

    From: $156 Sizes: 25 assays, 100 assaysCatalog #:
    30008-1
    , - 30008-2View allHide

    A fluorometric and colorimetric endpoint assay kit containing a DEVD substrate for detection of caspase-3 activity in cells.

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    25 assays
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    A fluorometric and colorimetric endpoint assay kit containing a DEVD substrate for detection of caspase-3 activity in cells.

     
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    Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit

    From: $51 Sizes: 10 assays, 100 assays, 1,000 assaysCatalog #:
    30009-1
    , 30009-2, - 30009-3View allHide

    A fluorometric endpoint assay kit for detection of caspase-3 activity in cells by high throughput screening (HTS).

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    10 assays
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    A fluorometric endpoint assay kit for detection of caspase-3 activity in cells by high throughput screening (HTS).

     
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    Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit

    From: $156 Sizes: 25 assays, 100 assaysCatalog #:
    30011-1
    , - 30011-2View allHide

    A fluorometric and colorimetric endpoint assay kit containing a IETD substrate for detection of caspase-8 activity in cells.

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    25 assays
    100 assays
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    A fluorometric and colorimetric endpoint assay kit containing a IETD substrate for detection of caspase-8 activity in cells.

     
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    Caspase-8 IETD-R110 Fluorometric HTS Assay Kit

    From: $51 Sizes: 10 assays, 100 assays, 1,000 assaysCatalog #:
    30012-1
    , 30012-2, - 30012-3View allHide

    A fluorometric endpoint assay kit for detection of caspase-8 activity in cells by high throughput screening (HTS).

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    10 assays
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    A fluorometric endpoint assay kit for detection of caspase-8 activity in cells by high throughput screening (HTS).

     
    Product

    D-Luciferin, Free Acid

    From: $64 Sizes: 10 mg, 50 mg, 1 gCatalog #:
    10100
    , 10100-1, - 10100-2View allHide

    D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.

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    10 mg
    50 mg
    1 g
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    D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.

     
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    D-Luciferin, Potassium Salt

    From: $64 Sizes: 10 mg, 50 mg, 1 gCatalog #:
    10101
    , 10101-1, - 10101-2View allHide

    D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.

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    10 mg
    50 mg
    1 g
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    D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.

     
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    D-Luciferin, Sodium Salt

    From: $74 Sizes: 10 mg, 50 mg, 1 gCatalog #:
    10102
    , 10102-1, - 10102-2View allHide

    D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.

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    10 mg
    50 mg
    1 g
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    D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase.

     
    Product

    DAB Substrate Kit

    From: $95 Sizes: 1 kitCatalog #: 30015

    DAB reacts with HRP in the presence of peroxide to yield an insoluble brown-colored product at locations where peroxidase-conjugated antibodies are bound to samples.

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    1 kit
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    DAB reacts with HRP in the presence of peroxide to yield an insoluble brown-colored product at locations where peroxidase-conjugated antibodies are bound to samples.

     
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    MTT Cell Viability Assay Kit

    From: $165 Sizes: 1,000 assaysCatalog #: 30006

    A simple, colorimetric assay for determining live cell numbers by absorbance on a microplate reader.

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    1,000 assays
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    A simple, colorimetric assay for determining live cell numbers by absorbance on a microplate reader.

     
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    XTT Cell Viability Assay Kit

    From: $189 Sizes: 1,000 assaysCatalog #: 30007
    A simple, colorimetric assay for determining live cell numbers by absorbance on a microplate reader.
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    1,000 assays
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    A simple, colorimetric assay for determining live cell numbers by absorbance on a microplate reader.
     
    Product

    Ac-DEVD-R110

    From: $95 Sizes: 1 mgCatalog #: 10226

    A fluorogenic substrate for caspase-3 that can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader.

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    1 mg
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    A fluorogenic substrate for caspase-3 that can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader.