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Enzyme Substrates

Biotium offers substrates for many common enzymes, including luminescent substrates for Firefly and Renilla Luciferases, luciferin and coelenterazine; fluorometric NucView® caspase-3 substrates; various protease substrates; and other enzyme substrates.

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    10-Acetyl-3,7-dihydroxyphenoxazine (Amplex® Red)

    From: $139 Sizes: 5 mgCatalog #: 10061

    10-Acetyl-3,7-dihydroxyphenoxazine (Amplex® Red), is regarded as the best fluorogenic substrate for peroxidase and H2O2 because it is highly sensitive, specific and stable.

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    10-Acetyl-3,7-dihydroxyphenoxazine (Amplex® Red), is regarded as the best fluorogenic substrate for peroxidase and H2O2 because it is highly sensitive, specific and stable.

     

    ABTS

    From: $116 Sizes: 1 gCatalog #: 10050

    ABTS (2,2′ -Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid), diammonium salt) is a very sensitive chromogenic peroxidase substrate widely used for ELISA applications. The substrate yields a water-soluble green colored product.

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    ABTS (2,2′ -Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid), diammonium salt) is a very sensitive chromogenic peroxidase substrate widely used for ELISA applications. The substrate yields a water-soluble green colored product.

     

    Ac-DEVD-AMC

    From: $210 Sizes: 5 mgCatalog #: 10202

    Ac-DEVD-AMC is a fluorogenic substrate for caspase 3 and can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader. For real-time detection of caspase-3 activity in intact cells, see our novel NucView®488 Caspase-3 Assay Kit.

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    Ac-DEVD-AMC is a fluorogenic substrate for caspase 3 and can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader. For real-time detection of caspase-3 activity in intact cells, see our novel NucView®488 Caspase-3 Assay Kit.

     

    Ac-IETD-R110

    From: $243 Sizes: 5 mgCatalog #: 10207

    Z-IETD-R110 is a substrate for caspase-8, which is involved at the early stage of apoptosis, acting as an initiator of the caspase activation cascade.

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    Z-IETD-R110 is a substrate for caspase-8, which is involved at the early stage of apoptosis, acting as an initiator of the caspase activation cascade.

     

    Aquaphile™ Coelenterazine h, Lyophilized Solid

    From: $105 Sizes: 50 ug, 5 x 100 ugCatalog #: 10127, - 10127-50ugView allHide

    Aquaphile coelenterazine h is a bioluminescence substrate in a special formulation format that is readily dissolved in water or PBS buffer for in vivo use.

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    Aquaphile coelenterazine h is a bioluminescence substrate in a special formulation format that is readily dissolved in water or PBS buffer for in vivo use.

     

    Aquaphile™ Coelenterazine Native, Lyophilized Solid

    From: $105 Sizes: 50 ug, 5 x 100 ugCatalog #: 10126, - 10126-50ugView allHide

    Aquaphile coelenterazine native is a bioluminescence substrate in a special formulation format that is readily dissolved in water or PBS buffer for in vivo use.

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    Aquaphile coelenterazine native is a bioluminescence substrate in a special formulation format that is readily dissolved in water or PBS buffer for in vivo use.

     

    BCIP Pink

    From: $139 Sizes: 100 mgCatalog #: 10006

    BCIP Pink (6-Chloro-3-indoxyl phosphate, p-toluidine salt) is a derivative of BCIP. While BCIP yields a blue precipitating product, BCIP Pink produces a pink colored (λmax 540 nm) precipitate.

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    BCIP Pink (6-Chloro-3-indoxyl phosphate, p-toluidine salt) is a derivative of BCIP. While BCIP yields a blue precipitating product, BCIP Pink produces a pink colored (λmax 540 nm) precipitate.

     

    BCIP Pink/NBT Kit

    From: $170 Sizes: 1 setCatalog #: 10007

    This kit contains 100 mg each of BCIP Pink (10006) and the precipitation inducer NBT (10008).

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    This kit contains 100 mg each of BCIP Pink (10006) and the precipitation inducer NBT (10008).

     

    BCIP Red

    From: $126 Sizes: 100 mgCatalog #: 10004

    BCIP Red (5-Bromo-6-chloro-3-indoxyl phosphate, p-toluidine salt ) is a derivative of BCIP. While BCIP yields a blue precipitating product, BCIP Red produces a red (λmax 565 nm) colored precipitate.

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    BCIP Red (5-Bromo-6-chloro-3-indoxyl phosphate, p-toluidine salt ) is a derivative of BCIP. While BCIP yields a blue precipitating product, BCIP Red produces a red (λmax 565 nm) colored precipitate.

     

    BCIP Red/NBT Kit

    From: $152 Sizes: 1 setCatalog #: 10005

    This kit contains 100 mg each of BCIP Red (10004) and the oxidant NBT (10008).

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    This kit contains 100 mg each of BCIP Red (10004) and the oxidant NBT (10008).

     

    BCIP, Na

    From: $60 Sizes: 100 mg, 500 mg, 5 gCatalog #: 10001, 10001-1, - 10001-2View allHide

    BCIP (5-Bromo-4-chloro-3-indoxyl phosphate, disodium salt) is the most widely used chromogenic phosphatase substrate, which forms a dark blue (λmax 615 nm) precipitate on enzymatic hydrolysis. It is often used with the oxidant NBT (nitro blue tetrazolium chloride catalog no. 10008), which facilitates the precipitation.

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    BCIP (5-Bromo-4-chloro-3-indoxyl phosphate, disodium salt) is the most widely used chromogenic phosphatase substrate, which forms a dark blue (λmax 615 nm) precipitate on enzymatic hydrolysis. It is often used with the oxidant NBT (nitro blue tetrazolium chloride catalog no. 10008), which facilitates the precipitation.

     

    BCIP, Toluidine

    From: $58 Sizes: 100 mg, 500 mg, 5 gCatalog #: 10002, 10002-1, - 10002-2View allHide

    BCIP, toluidine (5-Bromo-4-chloro-3-indoxyl phosphate, p-toluidine salt) is similar to BCIP/Na (10001).

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    BCIP, toluidine (5-Bromo-4-chloro-3-indoxyl phosphate, p-toluidine salt) is similar to BCIP/Na (10001).

     

    BCIP/NBT Kit

    From: $121 Sizes: 1 setCatalog #: 10003

    This kit contains 100 mg each of BCIP sodium salt (10001) and the oxidant NBT (Nitro blue tetrazolium, 10008).

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    This kit contains 100 mg each of BCIP sodium salt (10001) and the oxidant NBT (Nitro blue tetrazolium, 10008).

     

    BZAR

    From: $100 Sizes: 5 mgCatalog #: 10205

    BZAR (Rhodamine 110, bis-(N-CBZ-L-arginine amide), dihydrochloride) is a substrate for serine proteases (1-5) and is also an inhibitor for quanidinobenzoatase. It is 50 to 100 times more sensitive than the AMC-based substrate.

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    BZAR (Rhodamine 110, bis-(N-CBZ-L-arginine amide), dihydrochloride) is a substrate for serine proteases (1-5) and is also an inhibitor for quanidinobenzoatase. It is 50 to 100 times more sensitive than the AMC-based substrate.

     

    BZiPAR

    From: $166 Sizes: 5 mgCatalog #: 10208

    BZiPAR (Rhodamine 110, bis-(N-CBZ-L-isoleucyl-L-prolyl-L-arginine amide), dihydrochloride) is a substrate for trypsin (1,2). The substrate has been reported to enter live cells and be hydrolyzed by lysosomal proteases (3).

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    BZiPAR (Rhodamine 110, bis-(N-CBZ-L-isoleucyl-L-prolyl-L-arginine amide), dihydrochloride) is a substrate for trypsin (1,2). The substrate has been reported to enter live cells and be hydrolyzed by lysosomal proteases (3).

     

    Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit

    From: $139 Sizes: 25 assays, 100 assaysCatalog #: 30008-1, - 30008-2View allHide

    Caspase-3 is an active cell-death protease involved in the execution phase of apoptosis, during which cells undergo morphological changes such as DNA fragmentation, chromatin condensation and apoptotic body formation.

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    Caspase-3 is an active cell-death protease involved in the execution phase of apoptosis, during which cells undergo morphological changes such as DNA fragmentation, chromatin condensation and apoptotic body formation.