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PCR & Molecular Biology

Biotium offers high-quality reagents for many molecular biology applications:

PCR master mixes and related products; DNA & RNA isolation kits; DNA quantitation kits; nucleic acid gel stains; and more.

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    GelRed® Nucleic Acid Gel Stain

    From: $26 Sizes: Trial size (0.1 mL), 0.5 mL, 10 mL, 4 LCatalog #: 41001, 41002, 41003, 41002-1, 41003-1, - 41003-TView allHide

    GelRed® is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed® is far more sensitive than EB without requiring a destaining step.

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    GelRed® is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed® is far more sensitive than EB without requiring a destaining step.

     

    GelGreen® Nucleic Acid Gel Stain

    From: $22 Sizes: Trial size (0.1 mL), 0.5 mL, 10 mLCatalog #: 41004, 41005, 41005-1, - 41005-TView allHide

    GelGreen® is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye designed to stain either dsDNA, ssDNA or RNA in agarose gels. GelGreen® is far more sensitive than SYBR Safe.

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    GelGreen® is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye designed to stain either dsDNA, ssDNA or RNA in agarose gels. GelGreen® is far more sensitive than SYBR Safe.

     

    GelRed® Agarose LE

    Sizes: 5 g, 50 gCatalog #: 41029-5G, - 41029-50GView allHide

    GelRed® Agarose LE is  pre-coated with GelRed® Nucleic Acid Gel Stain for simple preparation of precast gels. With no need to handle concentrated fluorescent dye while preparing your gel, GelRed® Agarose offers greater convenience and safety.

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    GelRed® Agarose LE is  pre-coated with GelRed® Nucleic Acid Gel Stain for simple preparation of precast gels. With no need to handle concentrated fluorescent dye while preparing your gel, GelRed® Agarose offers greater convenience and safety.

     

    GelGreen® Agarose LE

    Sizes: 5 g, 50 gCatalog #: 41030-5G, - 41030-50GView allHide

    GelGreen® Agarose LE is  pre-coated with GelGreen® Nucleic Acid Gel Stain for simple preparation of precast gels. With no need to handle concentrated fluorescent dye while preparing your gel, GelGreen® Agarose offers greater convenience and safety.

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    GelGreen® Agarose LE is  pre-coated with GelGreen® Nucleic Acid Gel Stain for simple preparation of precast gels. With no need to handle concentrated fluorescent dye while preparing your gel, GelGreen® Agarose offers greater convenience and safety.

     

    6X DNA Loading Buffer (Blue)

    Sizes: 4 x 1.5 mLCatalog #: 99962-1

    6X DNA Loading Buffer (Blue) contains density agents for loading DNA samples on gels, with two blue electrophoresis tracking dyes.

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    4 x 1.5 mL
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    6X DNA Loading Buffer (Blue) contains density agents for loading DNA samples on gels, with two blue electrophoresis tracking dyes.

     

    6X GelRed® Prestain Loading Buffer with Tracking Dye

    From: $76 Sizes: 1 mLCatalog #: 41009, - 41010View allHide

    6X GelRed® Prestain Loading Buffers are DNA gel loading buffers containing GelRed® for one-step fluorescent staining and gel loading of DNA. Choose loading buffer with two blue tracking dyes or loading buffer with orange tracking dye.

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    1 mL
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    6X GelRed® Prestain Loading Buffers are DNA gel loading buffers containing GelRed® for one-step fluorescent staining and gel loading of DNA. Choose loading buffer with two blue tracking dyes or loading buffer with orange tracking dye.

     

    Agarose LE, Ultra-Pure Molecular Biology Grade

    Sizes: 25 g, 100 g, 500 gCatalog #: 41028-25G, 41028-100G, - 41028-500GView allHide

    High performance agarose for nucleic acid electrophoresis (analytical or preparative) and blotting. Validated for use with Biotium’s GelRed®, GelGreen®, and DNAzure® gel stains.

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    High performance agarose for nucleic acid electrophoresis (analytical or preparative) and blotting. Validated for use with Biotium’s GelRed®, GelGreen®, and DNAzure® gel stains.

     

    Ready-to-Use 1 kb DNA Ladder

    Sizes: 150 applications (1.5 mL)Catalog #: 31022

    1 kb DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 250 bp to 10 kb. The ladder consists of 13 bands that are generated from PCR and restriction enzyme digestion of double stranded DNA.

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    150 applications (1.5 mL)
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    1 kb DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 250 bp to 10 kb. The ladder consists of 13 bands that are generated from PCR and restriction enzyme digestion of double stranded DNA.

     

    Ready-to-Use 100 bp DNA Ladder

    Sizes: 150 applications (1.5 mL)Catalog #: 31032

    100 bp DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 100 bp to 1500 bp. The 500 bp and 1,500 bp bands contain more DNA to provide internal orientation.

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    150 applications (1.5 mL)
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    100 bp DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 100 bp to 1500 bp. The 500 bp and 1,500 bp bands contain more DNA to provide internal orientation.

     

    PAGE GelRed® Nucleic Acid Gel Stain

    From: $22 Sizes: 0.1 mL, 500 uL, 4 LCatalog #: 41014, 41008-500uL, - 41008-TView allHide

    PAGE GelRed® is a non-toxic, non-mutagenic red DNA gel stain specifically designed to stain DNA in polyacrylamide gels. PAGE GelRed® can be imaged using a 254 nm UV transilluminator with an ethidium bromide filter.

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    0.1 mL
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    4 L
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    PAGE GelRed® is a non-toxic, non-mutagenic red DNA gel stain specifically designed to stain DNA in polyacrylamide gels. PAGE GelRed® can be imaged using a 254 nm UV transilluminator with an ethidium bromide filter.

     

    Gel-Bright™ LED Gel Illuminator

    Sizes: EachCatalog #: E90003

    The Gel-Bright™ LED Gel Illuminator is a non-UV lightbox for detection of fluorescently labeled nucleic acids and protein.

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    The Gel-Bright™ LED Gel Illuminator is a non-UV lightbox for detection of fluorescently labeled nucleic acids and protein.

     

    DNA Gel Extraction Kit

    Sizes: 50 assays, 250 assaysCatalog #: 31030-250, - 31030-50View allHide

    Biotium’s DNA Gel Extraction Kit is a silica-gel spin column based DNA extraction kit designed to purify 40 bp-40 kb DNA fragments from agarose gels in TAE or TBE buffer. Each column has a maximum binding capacity of 10 ug DNA.

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    Biotium’s DNA Gel Extraction Kit is a silica-gel spin column based DNA extraction kit designed to purify 40 bp-40 kb DNA fragments from agarose gels in TAE or TBE buffer. Each column has a maximum binding capacity of 10 ug DNA.

     

    TBE Buffer, 5X

    From: $55 Sizes: 4 LCatalog #: 41006

    5X TBE (Tris-Borate-EDTA) is a concentrated buffer solution in deionized water. TBE is used for polyacrylamide and agarose gel electrophoresis.

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    4 L
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    5X TBE (Tris-Borate-EDTA) is a concentrated buffer solution in deionized water. TBE is used for polyacrylamide and agarose gel electrophoresis.

     

    RedDot™2 Far-Red Nuclear Stain, 200X in DMSO

    From: $68 Sizes: 25 uL (15-20 tests, trial size), 250 uL (150-200 tests), 1 mL (600-1000 tests)Catalog #: 40061, 40061-1, - 40061-TView allHide

    RedDot™2 is a far-red cell membrane-impermeable nuclear dye similar to Draq7™, with excitation and emission at 665/695 nm. The dye is ideal for specifically staining the nuclei of dead cells with minimal cytoplasmic RNA staining.

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    25 uL (15-20 tests, trial size)
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    RedDot™2 is a far-red cell membrane-impermeable nuclear dye similar to Draq7™, with excitation and emission at 665/695 nm. The dye is ideal for specifically staining the nuclei of dead cells with minimal cytoplasmic RNA staining.

     

    AccuBlue® NextGen dsDNA Quantitation Kit

    Sizes: Trial size (200 assays), 1,000 assaysCatalog #: 31060, - 31060-TView allHide

    The AccuBlue® NextGen dsDNA Quantitation Kit provides an unprecedented level of sensitivity in dsDNA detection. The AccuBlue® NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA.

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    The AccuBlue® NextGen dsDNA Quantitation Kit provides an unprecedented level of sensitivity in dsDNA detection. The AccuBlue® NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA.

     

    AccuBlue® NextGen dsDNA Quantitation Solution

    Sizes: Trial size (200 assays), 1,000 assaysCatalog #: 31061, - 31061-TView allHide

    The AccuBlue® NextGen dsDNA Quantitation Solution provides an unprecedented level of sensitivity in dsDNA detection. The AccuBlue® NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA.

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    The AccuBlue® NextGen dsDNA Quantitation Solution provides an unprecedented level of sensitivity in dsDNA detection. The AccuBlue® NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA.