PCR & Molecular Biology
Biotium offers high-quality reagents for many molecular biology applications:
PCR master mixes and related products; DNA & RNA isolation kits; DNA quantitation kits; nucleic acid gel stains; and more.
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1 kb DNA Ladder in TE Buffer
The 1 kb DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 250 bp to 10 kb. The ladder is composed of chromatography-purified DNA fragments dissolved in TE buffer (10 mM Tris pH 7.5, 1 mM EDTA). The 1 kb, 3 kb, and 6 kb bands contain more DNA to provide internal orientation.
The 1 kb DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 250 bp to 10 kb. The ladder is composed of chromatography-purified DNA fragments dissolved in TE buffer (10 mM Tris pH 7.5, 1 mM EDTA). The 1 kb, 3 kb, and 6 kb bands contain more DNA to provide internal orientation.
100 bp DNA Ladder in TE Buffer
The 100 bp DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 100 bp to 1000 bp. The ladder is composed of chromatography-purified DNA fragments dissolved in TE buffer (10 mM Tris pH 7.5, 1 mM EDTA). The 500 bp band contains more DNA to provide internal orientation.
The 100 bp DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 100 bp to 1000 bp. The ladder is composed of chromatography-purified DNA fragments dissolved in TE buffer (10 mM Tris pH 7.5, 1 mM EDTA). The 500 bp band contains more DNA to provide internal orientation.
5-Aminoallyl-dUTP (AA-dUTP), 10 mM solution
5-Aminoallyl-dUTP (AA-dUTP) can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling method (1,2).
5-Aminoallyl-dUTP (AA-dUTP) can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling method (1,2).
5-Aminoallyl-dUTP (AA-dUTP), Lyophilized Powder
5-Aminoallyl-dUTP (AA-dUTP) in lyophilized form suitable for long-term storage. 5-Aminoallyl-dUTP can be enzymatically incorporated into DNA.
5-Aminoallyl-dUTP (AA-dUTP) in lyophilized form suitable for long-term storage. 5-Aminoallyl-dUTP can be enzymatically incorporated into DNA.
5-Aminoallyl-UTP, Sodium Salt, 10 mM Solution
Alternate name(s): 5-(3-Aminoallyl)uridine-5′-triphosphate, trisodium salt; AA-UTP. 5-Aminoallyl-UTP can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling methods.
Alternate name(s): 5-(3-Aminoallyl)uridine-5′-triphosphate, trisodium salt; AA-UTP. 5-Aminoallyl-UTP can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling methods.
5-Aminoallyl-UTP, Sodium Salt, Lyophilized Powder
Alternate name(s): 5-(3-Aminoallyl)uridine-5′-triphosphate, trisodium salt; AA-UTP 5-Aminoallyl-UTP can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling methods.
Alternate name(s): 5-(3-Aminoallyl)uridine-5′-triphosphate, trisodium salt; AA-UTP 5-Aminoallyl-UTP can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling methods.
5-Bromo-dUTP, 10 mM solution
5-Bromo-dUTP (5-Bromo-2′-deoxyuridine-5′-triphosphate) is widely used in TUNEL assay to detect apoptosis cells (1,2), and it is also a good substrate for reverse transcriptase (3,4).
5-Bromo-dUTP (5-Bromo-2′-deoxyuridine-5′-triphosphate) is widely used in TUNEL assay to detect apoptosis cells (1,2), and it is also a good substrate for reverse transcriptase (3,4).
5-TAMRA-dUTP, 1 mM Solution
5-TAMRA-dUTP (5-Tetramethylrhodamine-dUTP) can be enzymatically incorporated into DNA. Note: 5-TAMRA-dUTP may not be compatible with PCR labeling of DNA probes.
5-TAMRA-dUTP (5-Tetramethylrhodamine-dUTP) can be enzymatically incorporated into DNA. Note: 5-TAMRA-dUTP may not be compatible with PCR labeling of DNA probes.
6X DNA Loading Buffer (Blue)
6X DNA Loading Buffer (Blue) contains density agents for loading DNA samples on gels, with two blue electrophoresis tracking dyes.
6X DNA Loading Buffer (Blue) contains density agents for loading DNA samples on gels, with two blue electrophoresis tracking dyes.
6X GelRed® Prestain Loading Buffer with Tracking Dye
6X GelRed® Prestain Loading Buffers are DNA gel loading buffers containing GelRed® for one-step fluorescent staining and gel loading of DNA. Choose loading buffer with two blue tracking dyes or loading buffer with orange tracking dye.
6X GelRed® Prestain Loading Buffers are DNA gel loading buffers containing GelRed® for one-step fluorescent staining and gel loading of DNA. Choose loading buffer with two blue tracking dyes or loading buffer with orange tracking dye.
7-AAD
7-AAD (7-aminoactinomycin D) is a fluorescent DNA binding dye that is membrane impermeant and therefore generally excluded from viable cells.
7-AAD (7-aminoactinomycin D) is a fluorescent DNA binding dye that is membrane impermeant and therefore generally excluded from viable cells.
AccuBlue® Broad Range dsDNA Quantitation Kit with DNA Standards
The AccuBlue® Broad Range dsDNA Quantitation Kit is designed for use with fluorescent plate readers in a 96-well microplate format. The AccuBlue® Broad Range Kit is specific for dsDNA, and can quantify DNA samples in the range of 0.2-100 ng/uL.
The AccuBlue® Broad Range dsDNA Quantitation Kit is designed for use with fluorescent plate readers in a 96-well microplate format. The AccuBlue® Broad Range Kit is specific for dsDNA, and can quantify DNA samples in the range of 0.2-100 ng/uL.
AccuBlue® Broad Range dsDNA Quantitation Solution
The AccuBlue® Broad Range dsDNA Quantitation Kit is designed for use with fluorescent plate readers in a 96-well microplate format. The AccuBlue® Broad Range Kit is specific for dsDNA, and can quantify DNA samples in the range of 0.2-100 ng/uL.
The AccuBlue® Broad Range dsDNA Quantitation Kit is designed for use with fluorescent plate readers in a 96-well microplate format. The AccuBlue® Broad Range Kit is specific for dsDNA, and can quantify DNA samples in the range of 0.2-100 ng/uL.
AccuBlue® Broad Range dsDNA Standards, Set of Nine
Stand-alone set of the nine double-stranded DNA standards provided with the AccuBlue® Broad Range dsDNA Quantitation Kit (31007). Calf thymus dsDNA in 10 mM Tris pH 7.5, 1 mM EDTA, 2 mM sodium azide at 0, 2, 6.25, 12.5, 25, 50, 100, 150, and 200 ng/uL, 0.5 mL each.
Stand-alone set of the nine double-stranded DNA standards provided with the AccuBlue® Broad Range dsDNA Quantitation Kit (31007). Calf thymus dsDNA in 10 mM Tris pH 7.5, 1 mM EDTA, 2 mM sodium azide at 0, 2, 6.25, 12.5, 25, 50, 100, 150, and 200 ng/uL, 0.5 mL each.
AccuBlue® High Sensitivity dsDNA Quantitation Kit with DNA Standards
AccuBlue® High Sensitivity is a non-mutagenic fluorescent dsDNA quantitation assay for fluorescence microplate reader. The kit offers sensitive and selective detection of purified dsDNA samples with excellent linearity.
AccuBlue® High Sensitivity is a non-mutagenic fluorescent dsDNA quantitation assay for fluorescence microplate reader. The kit offers sensitive and selective detection of purified dsDNA samples with excellent linearity.
AccuBlue® High Sensitivity dsDNA Quantitation Solution
The AccuBlue® High Sensitivity dsDNA Quantitation Solution offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants. The solution is intended for use on a fluorecent plate reader in a 96-well microplate format.
The AccuBlue® High Sensitivity dsDNA Quantitation Solution offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants. The solution is intended for use on a fluorecent plate reader in a 96-well microplate format.