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PCR & Molecular Biology

Biotium offers high-quality reagents for many molecular biology applications:

PCR master mixes and related products; DNA & RNA isolation kits; DNA quantitation kits; nucleic acid gel stains; and more.

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    1 kb DNA Ladder in TE Buffer

    Sizes: 30 ug/300 uLCatalog #: 31039

    The 1 kb DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 250 bp to 10 kb. The ladder is composed of chromatography-purified DNA fragments dissolved in TE buffer (10 mM Tris pH 7.5, 1 mM EDTA). The 1 kb, 3 kb, and 6 kb bands contain more DNA to provide internal orientation.

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    30 ug/300 uL
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    The 1 kb DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 250 bp to 10 kb. The ladder is composed of chromatography-purified DNA fragments dissolved in TE buffer (10 mM Tris pH 7.5, 1 mM EDTA). The 1 kb, 3 kb, and 6 kb bands contain more DNA to provide internal orientation.

     

    100 bp DNA Ladder in TE Buffer

    Sizes: 30 ug/300 uLCatalog #: 31040

    The 100 bp DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 100 bp to 1000 bp. The ladder is composed of chromatography-purified DNA fragments dissolved in TE buffer (10 mM Tris pH 7.5, 1 mM EDTA). The 500 bp band contains more DNA to provide internal orientation.

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    The 100 bp DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 100 bp to 1000 bp. The ladder is composed of chromatography-purified DNA fragments dissolved in TE buffer (10 mM Tris pH 7.5, 1 mM EDTA). The 500 bp band contains more DNA to provide internal orientation.

     

    5-Aminoallyl-dUTP (AA-dUTP), 10 mM solution

    From: $133 Sizes: 100 uLCatalog #: 40020

    5-Aminoallyl-dUTP (AA-dUTP) can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling method (1,2).

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    100 uL
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    5-Aminoallyl-dUTP (AA-dUTP) can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling method (1,2).

     

    5-Aminoallyl-dUTP (AA-dUTP), Lyophilized Powder

    From: $162 Sizes: 1 mgCatalog #: 40020-1

    5-Aminoallyl-dUTP (AA-dUTP) in lyophilized form suitable for long-term storage. 5-Aminoallyl-dUTP can be enzymatically incorporated into DNA.

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    5-Aminoallyl-dUTP (AA-dUTP) in lyophilized form suitable for long-term storage. 5-Aminoallyl-dUTP can be enzymatically incorporated into DNA.

     

    5-Aminoallyl-UTP, Sodium Salt, 10 mM Solution

    From: $133 Sizes: 100 uLCatalog #: 40021

    Alternate name(s): 5-(3-Aminoallyl)uridine-5′-triphosphate, trisodium salt; AA-UTP. 5-Aminoallyl-UTP can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling methods.

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    100 uL
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    Alternate name(s): 5-(3-Aminoallyl)uridine-5′-triphosphate, trisodium salt; AA-UTP. 5-Aminoallyl-UTP can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling methods.

     

    5-Aminoallyl-UTP, Sodium Salt, Lyophilized Powder

    From: $162 Sizes: 1 mgCatalog #: 40021-1

    Alternate name(s): 5-(3-Aminoallyl)uridine-5′-triphosphate, trisodium salt; AA-UTP 5-Aminoallyl-UTP can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling methods.

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    1 mg
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    Alternate name(s): 5-(3-Aminoallyl)uridine-5′-triphosphate, trisodium salt; AA-UTP 5-Aminoallyl-UTP can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling methods.

     

    5-Bromo-dUTP, 10 mM solution

    From: $87 Sizes: 25 uLCatalog #: 40025

    5-Bromo-dUTP (5-Bromo-2′-deoxyuridine-5′-triphosphate) is widely used in TUNEL assay to detect apoptosis cells (1,2), and it is also a good substrate for reverse transcriptase (3,4).

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    25 uL
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    5-Bromo-dUTP (5-Bromo-2′-deoxyuridine-5′-triphosphate) is widely used in TUNEL assay to detect apoptosis cells (1,2), and it is also a good substrate for reverse transcriptase (3,4).

     

    5-TAMRA-dUTP, 1 mM Solution

    From: $116 Sizes: 25 uLCatalog #: 40001

    5-TAMRA-dUTP (5-Tetramethylrhodamine-dUTP) can be enzymatically incorporated into DNA. Note: 5-TAMRA-dUTP may not be compatible with PCR labeling of DNA probes.

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    25 uL
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    5-TAMRA-dUTP (5-Tetramethylrhodamine-dUTP) can be enzymatically incorporated into DNA. Note: 5-TAMRA-dUTP may not be compatible with PCR labeling of DNA probes.

     

    6X DNA Loading Buffer (Blue)

    From: $50 Sizes: 4 x 1.5 mLCatalog #: 99962-1

    6X DNA Loading Buffer (Blue) contains density agents for loading DNA samples on gels, with two blue electrophoresis tracking dyes.

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    4 x 1.5 mL
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    6X DNA Loading Buffer (Blue) contains density agents for loading DNA samples on gels, with two blue electrophoresis tracking dyes.

     

    6X GelRed® Prestain Loading Buffer with Tracking Dye

    From: $76 Sizes: 1 mLCatalog #: 41009, - 41010View allHide

    6X GelRed® Prestain Loading Buffers are DNA gel loading buffers containing GelRed® for one-step fluorescent staining and gel loading of DNA. Choose loading buffer with two blue tracking dyes or loading buffer with orange tracking dye.

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    1 mL
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    6X GelRed® Prestain Loading Buffers are DNA gel loading buffers containing GelRed® for one-step fluorescent staining and gel loading of DNA. Choose loading buffer with two blue tracking dyes or loading buffer with orange tracking dye.

     

    7-AAD

    From: $110 Sizes: 1 mgCatalog #: 40037

    7-AAD (7-aminoactinomycin D) is a fluorescent DNA binding dye that is membrane impermeant and therefore generally excluded from viable cells.

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    7-AAD (7-aminoactinomycin D) is a fluorescent DNA binding dye that is membrane impermeant and therefore generally excluded from viable cells.

     

    AccuBlue® Broad Range dsDNA Quantitation Kit with DNA Standards

    From: $53 Sizes: 1500 assays, Trial size (200 assays)Catalog #: 31007-T, - 31007View allHide

    The AccuBlue® Broad Range dsDNA Quantitation Kit is designed for use with fluorescent plate readers in a 96-well microplate format. The AccuBlue® Broad Range Kit is specific for dsDNA, and can quantify DNA samples in the range of 0.2-100 ng/uL.

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    1500 assays
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    The AccuBlue® Broad Range dsDNA Quantitation Kit is designed for use with fluorescent plate readers in a 96-well microplate format. The AccuBlue® Broad Range Kit is specific for dsDNA, and can quantify DNA samples in the range of 0.2-100 ng/uL.

     

    AccuBlue® Broad Range dsDNA Quantitation Solution

    From: $42 Sizes: 1500 assays, Trial size (200 assays)Catalog #: 31009, - 31009-TView allHide

    The AccuBlue® Broad Range dsDNA Quantitation Kit is designed for use with fluorescent plate readers in a 96-well microplate format. The AccuBlue® Broad Range Kit is specific for dsDNA, and can quantify DNA samples in the range of 0.2-100 ng/uL.

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    1500 assays
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    The AccuBlue® Broad Range dsDNA Quantitation Kit is designed for use with fluorescent plate readers in a 96-well microplate format. The AccuBlue® Broad Range Kit is specific for dsDNA, and can quantify DNA samples in the range of 0.2-100 ng/uL.

     

    AccuBlue® Broad Range dsDNA Standards, Set of Nine

    From: $79 Sizes: 0.5 mL eachCatalog #: 31007C

    Stand-alone set of the nine double-stranded DNA standards provided with the AccuBlue® Broad Range dsDNA Quantitation Kit (31007). Calf thymus dsDNA in 10 mM Tris pH 7.5, 1 mM EDTA, 2 mM sodium azide at 0, 2, 6.25, 12.5, 25, 50, 100, 150, and 200 ng/uL, 0.5 mL each.

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    0.5 mL each
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    Stand-alone set of the nine double-stranded DNA standards provided with the AccuBlue® Broad Range dsDNA Quantitation Kit (31007). Calf thymus dsDNA in 10 mM Tris pH 7.5, 1 mM EDTA, 2 mM sodium azide at 0, 2, 6.25, 12.5, 25, 50, 100, 150, and 200 ng/uL, 0.5 mL each.

     

    AccuBlue® High Sensitivity dsDNA Quantitation Kit with DNA Standards

    From: $53 Sizes: Trial size (200 assays), 1,000 assaysCatalog #: 31006, - 31006-TView allHide

    AccuBlue® High Sensitivity is a non-mutagenic fluorescent dsDNA quantitation assay for fluorescence microplate reader. The kit offers sensitive and selective detection of purified dsDNA samples with excellent linearity.

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    1,000 assays
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    AccuBlue® High Sensitivity is a non-mutagenic fluorescent dsDNA quantitation assay for fluorescence microplate reader. The kit offers sensitive and selective detection of purified dsDNA samples with excellent linearity.

     

    AccuBlue® High Sensitivity dsDNA Quantitation Solution

    From: $42 Sizes: Trial size, 1,000 assaysCatalog #: 31008, - 31008-TView allHide

    The AccuBlue®  High Sensitivity dsDNA Quantitation Solution offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants. The solution is intended for use on a fluorecent plate reader in a 96-well microplate format.

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    The AccuBlue®  High Sensitivity dsDNA Quantitation Solution offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants. The solution is intended for use on a fluorecent plate reader in a 96-well microplate format.