qPCR Reagents
The Forget-Me-Not™ series of qPCR master mixes are robust, sensitive, and affordable master mixes for all of your qPCR needs. For DNA dye-based qPCR, the Forget-Me-Not™ EvaGreen® master mixes are an excellent choice, and are offered in two formats: basic, and with two-color tracking to reduce pipetting errors. For probe-based qPCR, the Forget-Me-Not™ Universal Probe master mix is ideal for all probe types, such as TaqMan® or Molecular Beacons.
We also offer PCR accessory products such as dNTPs, Cheetah™ Taq HotStart Polymerase, EvaGreen® PCR dye, ROX reference dye, and the EvaEZ™ polymerase activity assay.
To learn about microbial viability PCR using PMA and PMAxx™ viability dyes, visit the viability PCR technology page.
Cheetah™ HotStart Taq DNA Polymerase
Cheetah™ Taq is a chemically modified hot-start Taq DNA polymerase useful for preventing or reducing nonspecific DNA amplification in PCR. Modified using a novel modifying reagent, Cheetah™ Taq represents a major improvement upon AmpliTaq® Gold by having a faster activation time and better shelf-life.
Cheetah™ Taq is a chemically modified hot-start Taq DNA polymerase useful for preventing or reducing nonspecific DNA amplification in PCR. Modified using a novel modifying reagent, Cheetah™ Taq represents a major improvement upon AmpliTaq® Gold by having a faster activation time and better shelf-life.
EvaEZ™ Fluorometric Polymerase Activity Assay Kit
An easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes.
An easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes.
EvaGreen® Dye, 20X in Water
A green fluorescent nucleic acid dye with features that are ideal for applications such as qPCR, HRM®, LAMP, and digital PCR.
A green fluorescent nucleic acid dye with features that are ideal for applications such as qPCR, HRM®, LAMP, and digital PCR.
EvaGreen® Plus Dye, 20X in Water
EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.
EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.
Forget-Me-Not™ EvaGreen® qPCR Master Mix (2-Color Tracking)
A high-performance dye-based qPCR master mix containing EvaGreen®, Cheetah™ HotStart Taq Polymerase, and the Forget-Me-Not™ 2-color tracking system.
A high-performance dye-based qPCR master mix containing EvaGreen®, Cheetah™ HotStart Taq Polymerase, and the Forget-Me-Not™ 2-color tracking system.
Forget-Me-Not™ EvaGreen® qPCR Master Mix (Low ROX or High ROX)
A high-performance dye-based qPCR master mix containing EvaGreen®, premixed ROX, Cheetah™ HotStart Taq Polymerase, and a low concentration of the Forget-Me-Not™ inert blue tracking dye.
A high-performance dye-based qPCR master mix containing EvaGreen®, premixed ROX, Cheetah™ HotStart Taq Polymerase, and a low concentration of the Forget-Me-Not™ inert blue tracking dye.
Forget-Me-Not™ Universal Probe qPCR Master Mix
A high-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping.
A high-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping.
PMA Enhancer for Gram Negative Bacteria, 5X Solution
This product improves PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria when performing viability PCR.
This product improves PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria when performing viability PCR.
PMA Real-Time PCR Bacterial Viability Kit – E. coli (uidA)
A kit designed to perform viability PCR on E. coli. It contains a viability dye (PMAxx™ or PMA) and primers to amplify the uidA gene.
A kit designed to perform viability PCR on E. coli. It contains a viability dye (PMAxx™ or PMA) and primers to amplify the uidA gene.
PMA Real-Time PCR Bacterial Viability Kit – E. coli O157:H7 (Z3276)
A kit designed to perform viability PCR on E. coli O157:H7. It contains a viability PCR dye (PMAxx™ or PMA) and primers to amplify the Z3276 gene.
A kit designed to perform viability PCR on E. coli O157:H7. It contains a viability PCR dye (PMAxx™ or PMA) and primers to amplify the Z3276 gene.
PMA Real-Time PCR Bacterial Viability Kit – Legionella pneumophila (mip)
A kit designed to perform viability PCR on Legionella pneumophila. It contains the viability PCR dye PMA and primers to amplify the mip gene.
A kit designed to perform viability PCR on Legionella pneumophila. It contains the viability PCR dye PMA and primers to amplify the mip gene.
PMA Real-Time PCR Bacterial Viability Kit – Listeria monocytogenes (hly)
A kit designed to perform viability PCR on Listeria monocytogenes. It contains a viability dye (PMAxx™ or PMA) and primers to amplify the hly gene.
A kit designed to perform viability PCR on Listeria monocytogenes. It contains a viability dye (PMAxx™ or PMA) and primers to amplify the hly gene.
PMA Real-Time PCR Bacterial Viability Kit – Mycobacterium tuberculosis (groEL2)
A kit designed to perform viability PCR on Mycobacterium tuberculosis. It contains the viability PCR dye PMA and primers to amplify the groEL2 gene.
A kit designed to perform viability PCR on Mycobacterium tuberculosis. It contains the viability PCR dye PMA and primers to amplify the groEL2 gene.
PMA Real-Time PCR Bacterial Viability Kit – Salmonella enterica (invA)
A kit designed to perform viability PCR on Salmonella enterica. It contains a viability PCR dye (PMAxx™ or PMA) and primers to amplify the invA gene.
A kit designed to perform viability PCR on Salmonella enterica. It contains a viability PCR dye (PMAxx™ or PMA) and primers to amplify the invA gene.
PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (mecA)
A kit designed to perform viability PCR on MRSA. It contains the viability PCR dye PMA and primers to amplify the mecA gene.
A kit designed to perform viability PCR on MRSA. It contains the viability PCR dye PMA and primers to amplify the mecA gene.
PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (nuc)
A kit designed to perform viability PCR on Staphylococcus aureus. It contains the viability PCR dye PMA and primers to amplify the nuc gene.
A kit designed to perform viability PCR on Staphylococcus aureus. It contains the viability PCR dye PMA and primers to amplify the nuc gene.