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qPCR Reagents

The Forget-Me-Not™ series of qPCR master mixes are robust, sensitive, and affordable master mixes for all of your qPCR needs. For DNA dye-based qPCR, the Forget-Me-Not™ EvaGreen® master mixes are an excellent choice, and are offered in two formats: basic, and with two-color tracking to reduce pipetting errors. For probe-based qPCR, the Forget-Me-Not™ Universal Probe master mix is ideal for all probe types, such as TaqMan® or Molecular Beacons.

We also offer PCR accessory products such as dNTPs, Cheetah™ Taq HotStart Polymerase, EvaGreen® PCR dye, ROX reference dye, and the EvaEZ™ polymerase activity assay.

To learn about microbial viability PCR using PMA and PMAxx™ viability dyes, visit the viability PCR technology page.

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    Cheetah™ HotStart Taq DNA Polymerase

    From: $221 Sizes: 500 UCatalog #: 29050

    Cheetah™ Taq is a chemically modified hot-start Taq DNA polymerase useful for preventing or reducing nonspecific DNA amplification in PCR. Modified using a novel modifying reagent, Cheetah™ Taq represents a major improvement upon AmpliTaq® Gold by having a faster activation time and better shelf-life.

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    500 U
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    Cheetah™ Taq is a chemically modified hot-start Taq DNA polymerase useful for preventing or reducing nonspecific DNA amplification in PCR. Modified using a novel modifying reagent, Cheetah™ Taq represents a major improvement upon AmpliTaq® Gold by having a faster activation time and better shelf-life.

     

    EvaEZ™ Fluorometric Polymerase Activity Assay Kit

    From: $368 Sizes: 2 x 1 mLCatalog #: 29051

    EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes. It contains EvaGreen® dye together with a primed template, dNTPs, and MgCl2 in a Tris buffer system. Available without magnesium by special order.

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    2 x 1 mL
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    EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes. It contains EvaGreen® dye together with a primed template, dNTPs, and MgCl2 in a Tris buffer system. Available without magnesium by special order.

     

    EvaGreen® Dye, 20X in Water

    From: $44 Sizes: Trial size (1 mL), 5 x 1 mLCatalog #: 31000, - 31000-TView allHide

    EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis.

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    EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis.

     

    Forget-Me-Not™ EvaGreen® qPCR Master Mix (2-Color Tracking)

    From: $58 Sizes: 1 mL (100 reactions) Trial Size, 5 x 1 mL (500 reactions), 2 x 10 mL (2000 reactions)Catalog #: , , 31041-T, 31041-1, 31041-20mL, 31042-T, 31042-1, - 31042-20mLView allHide

    Forget-Me-Not™ EvaGreen® is a high performance qPCR master mix with 2-color reaction component tracking system available for a fraction of the cost of similar products.

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    1 mL (100 reactions) Trial Size
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    Forget-Me-Not™ EvaGreen® is a high performance qPCR master mix with 2-color reaction component tracking system available for a fraction of the cost of similar products.

     

    Forget-Me-Not™ EvaGreen® qPCR Master Mix (Low ROX or High ROX)

    Sizes: 1 mL (100 reactions) Trial Size, 5 x 1 mL (500 reactions), 2 x 10 mL (2000 reactions)Catalog #: , , 31045-1mL, 31045-5mL, 31045-20mL, 31046-1mL, 31046-5mL, - 31046-20mLView allHide

    Forget-Me-Not™ EvaGreen® qPCR Master Mix is a hotstart EvaGreen® dye-based master mix for use in real time PCR applications and DNA melt curve analysis.

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    1 mL (100 reactions) Trial Size
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    Forget-Me-Not™ EvaGreen® qPCR Master Mix is a hotstart EvaGreen® dye-based master mix for use in real time PCR applications and DNA melt curve analysis.

     

    Forget-Me-Not™ Universal Probe qPCR Master Mix

    From: $60 Sizes: 1 mL (100 reactions) Trial Size, 5 x 1 mL (500 reactions)Catalog #: 31043-1, 31043-T, 31044-1, - 31044-TView allHide

    2X Forget-Me-Not™ Universal Probe qPCR Master Mix is a high-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping. This kit is suitable for all fluorescent probe-based technologies, including hydrolysis probes (such as TaqMan® and dual-labeled BHQ® probes) and displacement probes (like Molecular Beacons).

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    1 mL (100 reactions) Trial Size
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    2X Forget-Me-Not™ Universal Probe qPCR Master Mix is a high-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping. This kit is suitable for all fluorescent probe-based technologies, including hydrolysis probes (such as TaqMan® and dual-labeled BHQ® probes) and displacement probes (like Molecular Beacons).

     

    PMA Enhancer for Gram Negative Bacteria, 5X Solution

    From: $74 Sizes: 16 mLCatalog #: 31038

    PMA Enhancer for Gram Negative Bacteria was designed to improve PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria.

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    16 mL
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    PMA Enhancer for Gram Negative Bacteria was designed to improve PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria.

     

    PMA Real-Time PCR Bacterial Viability Kit – E. coli (uidA)

    From: $315 Sizes: 200 assaysCatalog #: 31050, - 31050-XView allHide

    This kit contains primers for amplification of E. coli uidA gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx, and perform 200 PCR reactions.

    Viability dye
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    200 assays
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    This kit contains primers for amplification of E. coli uidA gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx, and perform 200 PCR reactions.

     

    PMA Real-Time PCR Bacterial Viability Kit – E. coli O157:H7 (Z3276)

    From: $315 Sizes: 200 assaysCatalog #: 31037, - 31037-XView allHide

    This kit contains primers for amplification of E. coli strain O157:H7 Z3276 gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions.

    Viability dye
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    200 assays
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    This kit contains primers for amplification of E. coli strain O157:H7 Z3276 gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions.

     

    PMA Real-Time PCR Bacterial Viability Kit – Legionella pneumophila (mip)

    From: $315 Sizes: 200 assaysCatalog #: 31053

    This kit contains primers for amplification of Legionella pneumophila mip gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.

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    200 assays
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    This kit contains primers for amplification of Legionella pneumophila mip gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.

     

    PMA Real-Time PCR Bacterial Viability Kit – Listeria monocytogenes (hly)

    From: $315 Sizes: 200 assaysCatalog #: 31051, - 31051-XView allHide

    This kit contains primers for amplification of Listeria monocytogenes hly gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions.

    Viability dye
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    200 assays
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    This kit contains primers for amplification of Listeria monocytogenes hly gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions.

     

    PMA Real-Time PCR Bacterial Viability Kit – Mycobacterium tuberculosis (groEL2)

    From: $315 Sizes: 200 assaysCatalog #: 31034

    This kit contains primers for amplification of Mycobacterium tuberculosis groEL2 gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.

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    200 assays
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    This kit contains primers for amplification of Mycobacterium tuberculosis groEL2 gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.

     

    PMA Real-Time PCR Bacterial Viability Kit – Salmonella enterica (invA)

    From: $315 Sizes: 200 assaysCatalog #: 31033, - 31033-XView allHide

    This kit contains primers for amplification of Salmonella enterica invA gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions.

    Viability dye
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    200 assays
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    This kit contains primers for amplification of Salmonella enterica invA gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions.

     

    PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (mecA)

    From: $315 Sizes: 200 assaysCatalog #: 31036

    This kit contains primers for amplification of the methicillin resistance gene mecA, which confers methicillin-resistance in Staphyloccoccus aureus and other bacterial species. The kit includes reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.

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    200 assays
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    This kit contains primers for amplification of the methicillin resistance gene mecA, which confers methicillin-resistance in Staphyloccoccus aureus and other bacterial species. The kit includes reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.

     

    PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (nuc)

    From: $315 Sizes: 200 assaysCatalog #: 31035

    This kit contains primers for amplification of  Staphylococcus aureus-specific nuc gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.

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    200 assays
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    This kit contains primers for amplification of  Staphylococcus aureus-specific nuc gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.

     

    dNTP Mix, 10 mM Each

    From: $368 Sizes: 5 x 1 mLCatalog #: 40054

    Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 10 mM in the solution provided.

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    5 x 1 mL
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    Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 10 mM in the solution provided.