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qPCR Reagents

The Forget-Me-Not™ series of qPCR master mixes are robust, sensitive, and affordable master mixes for all of your qPCR needs. For DNA dye-based qPCR, the Forget-Me-Not™ EvaGreen® master mixes are an excellent choice, and are offered in two formats: basic, and with two-color tracking to reduce pipetting errors. For probe-based qPCR, the Forget-Me-Not™ Universal Probe master mix is ideal for all probe types, such as TaqMan® or Molecular Beacons.

We also offer PCR accessory products such as dNTPs, Cheetah™ Taq HotStart Polymerase, EvaGreen® PCR dye, ROX reference dye, and the EvaEZ™ polymerase activity assay.

To learn about microbial viability PCR using PMA and PMAxx™ viability dyes, visit the viability PCR technology page.

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    Cheetah™ HotStart Taq DNA Polymerase

    From: $228 Sizes: 500 UCatalog #: 29050

    Cheetah™ Taq is a chemically modified hot-start Taq DNA polymerase useful for preventing or reducing nonspecific DNA amplification in PCR. Modified using a novel modifying reagent, Cheetah™ Taq represents a major improvement upon AmpliTaq® Gold by having a faster activation time and better shelf-life.

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    Cheetah™ Taq is a chemically modified hot-start Taq DNA polymerase useful for preventing or reducing nonspecific DNA amplification in PCR. Modified using a novel modifying reagent, Cheetah™ Taq represents a major improvement upon AmpliTaq® Gold by having a faster activation time and better shelf-life.

     

    EvaEZ™ Fluorometric Polymerase Activity Assay Kit

    From: $379 Sizes: 2 x 1 mLCatalog #: 29051

    An easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes.

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    2 x 1 mL
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    An easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes.

     

    EvaGreen® Dye, 20X in Water

    From: $46 Sizes: Trial size (1 mL), 5 x 1 mLCatalog #: 31000, - 31000-TView allHide

    A green fluorescent nucleic acid dye with features that are ideal for applications such as qPCR, HRM®, LAMP, and digital PCR.

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    A green fluorescent nucleic acid dye with features that are ideal for applications such as qPCR, HRM®, LAMP, and digital PCR.

     

    EvaGreen® Plus Dye, 20X in Water

    From: $92 Sizes: Trial size (1 mL), 5 x 1 mLCatalog #: 31077-T, - 31077View allHide

    EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.

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    EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.

     

    Forget-Me-Not™ EvaGreen® qPCR Master Mix (2-Color Tracking)

    From: $60 Sizes: 1 mL (100 reactions) Trial Size, 5 x 1 mL (500 reactions), 2 x 10 mL (2000 reactions)Catalog #: 31041-T, 31041-1, 31041-20mL, 31042-T, 31042-1, - 31042-20mLView allHide

    A high-performance dye-based qPCR master mix containing EvaGreen®, Cheetah™ HotStart Taq Polymerase, and the Forget-Me-Not™ 2-color tracking system.

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    1 mL (100 reactions) Trial Size
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    A high-performance dye-based qPCR master mix containing EvaGreen®, Cheetah™ HotStart Taq Polymerase, and the Forget-Me-Not™ 2-color tracking system.

     

    Forget-Me-Not™ EvaGreen® qPCR Master Mix (Low ROX or High ROX)

    Sizes: 1 mL (100 reactions) Trial Size, 5 x 1 mL (500 reactions), 2 x 10 mL (2000 reactions)Catalog #: 31045-1mL, 31045-5mL, 31045-20mL, 31046-1mL, 31046-5mL, - 31046-20mLView allHide

    A high-performance dye-based qPCR master mix containing EvaGreen®, premixed ROX, Cheetah™ HotStart Taq Polymerase, and a low concentration of the Forget-Me-Not™ inert blue tracking dye.

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    1 mL (100 reactions) Trial Size
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    A high-performance dye-based qPCR master mix containing EvaGreen®, premixed ROX, Cheetah™ HotStart Taq Polymerase, and a low concentration of the Forget-Me-Not™ inert blue tracking dye.

     

    Forget-Me-Not™ Universal Probe qPCR Master Mix

    From: $60 Sizes: 1 mL (100 reactions) Trial Size, 5 x 1 mL (500 reactions)Catalog #: 31043-1, 31043-T, 31044-1, - 31044-TView allHide

    A high-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping.

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    A high-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping.

     

    PMA Enhancer for Gram Negative Bacteria, 5X Solution

    From: $76 Sizes: 16 mLCatalog #: 31038

    This product improves PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria when performing viability PCR.

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    16 mL
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    This product improves PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria when performing viability PCR.

     

    PMA Real-Time PCR Bacterial Viability Kit – E. coli (uidA)

    From: $350 Sizes: 200 assaysCatalog #: 31050, - 31050-XView allHide

    A kit designed to perform viability PCR on E. coli. It contains a viability dye (PMAxx™ or PMA) and primers to amplify the uidA gene.

    Viability dye
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    200 assays
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    A kit designed to perform viability PCR on E. coli. It contains a viability dye (PMAxx™ or PMA) and primers to amplify the uidA gene.

     

    PMA Real-Time PCR Bacterial Viability Kit – E. coli O157:H7 (Z3276)

    From: $350 Sizes: 200 assaysCatalog #: 31037, - 31037-XView allHide

    A kit designed to perform viability PCR on E. coli O157:H7. It contains a viability PCR dye (PMAxx™ or PMA) and primers to amplify the Z3276 gene.

    Viability dye
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    200 assays
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    A kit designed to perform viability PCR on E. coli O157:H7. It contains a viability PCR dye (PMAxx™ or PMA) and primers to amplify the Z3276 gene.

     

    PMA Real-Time PCR Bacterial Viability Kit – Legionella pneumophila (mip)

    From: $350 Sizes: 200 assaysCatalog #: 31053

    A kit designed to perform viability PCR on Legionella pneumophila. It contains the viability PCR dye PMA and primers to amplify the mip gene.

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    200 assays
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    A kit designed to perform viability PCR on Legionella pneumophila. It contains the viability PCR dye PMA and primers to amplify the mip gene.

     

    PMA Real-Time PCR Bacterial Viability Kit – Listeria monocytogenes (hly)

    From: $324 Sizes: 200 assaysCatalog #: 31051, - 31051-XView allHide

    A kit designed to perform viability PCR on Listeria monocytogenes. It contains a viability dye (PMAxx™ or PMA) and primers to amplify the hly gene.

    Viability dye
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    A kit designed to perform viability PCR on Listeria monocytogenes. It contains a viability dye (PMAxx™ or PMA) and primers to amplify the hly gene.

     

    PMA Real-Time PCR Bacterial Viability Kit – Mycobacterium tuberculosis (groEL2)

    From: $324 Sizes: 200 assaysCatalog #: 31034

    A kit designed to perform viability PCR on Mycobacterium tuberculosis. It contains the viability PCR dye PMA and primers to amplify the groEL2 gene.

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    200 assays
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    A kit designed to perform viability PCR on Mycobacterium tuberculosis. It contains the viability PCR dye PMA and primers to amplify the groEL2 gene.

     

    PMA Real-Time PCR Bacterial Viability Kit – Salmonella enterica (invA)

    From: $350 Sizes: 200 assaysCatalog #: 31033, - 31033-XView allHide

    A kit designed to perform viability PCR on Salmonella enterica. It contains a viability PCR dye (PMAxx™ or PMA) and primers to amplify the invA gene.

    Viability dye
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    A kit designed to perform viability PCR on Salmonella enterica. It contains a viability PCR dye (PMAxx™ or PMA) and primers to amplify the invA gene.

     

    PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (mecA)

    From: $324 Sizes: 200 assaysCatalog #: 31036

    A kit designed to perform viability PCR on MRSA. It contains the viability PCR dye PMA and primers to amplify the mecA gene.

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    200 assays
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    A kit designed to perform viability PCR on MRSA. It contains the viability PCR dye PMA and primers to amplify the mecA gene.

     

    PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (nuc)

    From: $324 Sizes: 200 assaysCatalog #: 31035

    A kit designed to perform viability PCR on Staphylococcus aureus. It contains the viability PCR dye PMA and primers to amplify the nuc gene.

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    200 assays
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    A kit designed to perform viability PCR on Staphylococcus aureus. It contains the viability PCR dye PMA and primers to amplify the nuc gene.