qPCR Reagents
The Forget-Me-Not™ series of qPCR master mixes are robust, sensitive, and affordable master mixes for all of your qPCR needs. For DNA dye-based qPCR, the Forget-Me-Not™ EvaGreen® master mixes are an excellent choice, and are offered in two formats: basic, and with two-color tracking to reduce pipetting errors. For probe-based qPCR, the Forget-Me-Not™ Universal Probe master mix is ideal for all probe types, such as TaqMan® or Molecular Beacons.
We also offer PCR accessory products such as dNTPs, Cheetah™ Taq HotStart Polymerase, EvaGreen® PCR dye, ROX reference dye, and the EvaEZ™ polymerase activity assay.
To learn about microbial viability PCR using PMA and PMAxx™ viability dyes, visit the viability PCR technology page.
Cheetah™ HotStart Taq DNA Polymerase
A hot-start Taq DNA polymerase modified using a novel modifying reagent, it has a faster activation time and better shelf-life.
A hot-start Taq DNA polymerase modified using a novel modifying reagent, it has a faster activation time and better shelf-life.
EvaEZ™ Fluorometric Polymerase Activity Assay Kit
An easy and accurate way to determine the activity of a DNA polymerase without using radioisotopes.
An easy and accurate way to determine the activity of a DNA polymerase without using radioisotopes.
EvaGreen® Dye, 20X in Water
31000, - 31000-TView allHide
A patented green fluorescent nucleic acid dye with features that are ideal for applications such as qPCR, HRM®, LAMP, and digital PCR.
A patented green fluorescent nucleic acid dye with features that are ideal for applications such as qPCR, HRM®, LAMP, and digital PCR.
EvaGreen® Plus Dye, 20X in Water
31077-T, - 31077View allHide
EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.
EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.
Forget-Me-Not™ EvaGreen® qPCR Master Mix (2-Color Tracking)
31041-T, 31041-1, 31041-20mL, 31042-T, 31042-1, - 31042-20mLView allHide
A high-performance dye-based qPCR master mix containing EvaGreen®, Cheetah™ HotStart Taq Polymerase, and the Forget-Me-Not™ 2-color tracking system.
A high-performance dye-based qPCR master mix containing EvaGreen®, Cheetah™ HotStart Taq Polymerase, and the Forget-Me-Not™ 2-color tracking system.
Forget-Me-Not™ EvaGreen® qPCR Master Mix (Low ROX or High ROX)
31045-1mL, 31045-5mL, 31045-20mL, 31046-1mL, 31046-5mL, - 31046-20mLView allHide
A high-performance dye-based qPCR master mix containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. With low or high ROX.
A high-performance dye-based qPCR master mix containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. With low or high ROX.
Forget-Me-Not™ Universal Probe qPCR Master Mix
31043-1, 31043-T, 31044-1, - 31044-TView allHide
A high-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping.
A high-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping.
N-Flux™ 5X Digital PCR Master Mix
31078-T, - 31078View allHide
A 5X master mix specially formulated to address the complexities of reagent flow through dPCR chips and plates to achieve optimal sample partitioning and maximum end-point fluorescence.
A 5X master mix specially formulated to address the complexities of reagent flow through dPCR chips and plates to achieve optimal sample partitioning and maximum end-point fluorescence.
dNTP Mix, 10 mM Each
Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 10 mM in the solution provided.
Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 10 mM in the solution provided.
dNTP Mix, 25 mM Each
Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 25 mM in the solution provided.
Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 25 mM in the solution provided.
dNTP Set, 100 mM Each
Highly purified dNTPs (>99% by HPLC) in purified water. Each deoxynucleotide solution (dATP, dCTP, dGTP, dTTP) is provided in a separate vial at 100 mM.
Highly purified dNTPs (>99% by HPLC) in purified water. Each deoxynucleotide solution (dATP, dCTP, dGTP, dTTP) is provided in a separate vial at 100 mM.
EvaGreen® Dye, 2000X in DMSO
A patented green fluorescent nucleic acid dye with features that are ideal for applications such as qPCR, HRM®, LAMP, and digital PCR. Highly concentrated for customers with specialized applications requiring a concentrated dye solution.
A patented green fluorescent nucleic acid dye with features that are ideal for applications such as qPCR, HRM®, LAMP, and digital PCR. Highly concentrated for customers with specialized applications requiring a concentrated dye solution.
Fast EvaGreen® qPCR Master Mix
31003, 31003-1, 31003-2, - 31003-TView allHide
A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.
A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.
Fast Plus EvaGreen® qPCR Master Mix
31014, 31015, 31020, 31014-1, 31014-2, 31014-T, 31015-1, 31015-2, 31015-T, 31020-1, 31020-2, - 31020-TView allHide
A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.
A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.
Fast Probe Master Mix
31005, 31016, 31005-1, 31005-2, 31005-T, 31016-1, 31016-2, - 31016-TView allHide
A qPCR master mix containing Cheetah™ Taq hotstart DNA polymerase, suitable for probe-based real-time PCR applications.
A qPCR master mix containing Cheetah™ Taq hotstart DNA polymerase, suitable for probe-based real-time PCR applications.
HotStart Polymerase Modification Kit
A simple kit for reversibly modifying thermostable DNA polymerases, rendering the enzyme inactive and preventing primer dimers during reaction set-up.
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A simple kit for reversibly modifying thermostable DNA polymerases, rendering the enzyme inactive and preventing primer dimers during reaction set-up.