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qPCR Reagents

The Forget-Me-Not™ series of qPCR master mixes are robust, sensitive, and affordable master mixes for all of your qPCR needs. For DNA dye-based qPCR, the Forget-Me-Not™ EvaGreen® master mixes are an excellent choice, and are offered in two formats: basic, and with two-color tracking to reduce pipetting errors. For probe-based qPCR, the Forget-Me-Not™ Universal Probe master mix is ideal for all probe types, such as TaqMan® or Molecular Beacons.

We also offer PCR accessory products such as dNTPs, Cheetah™ Taq HotStart Polymerase, EvaGreen® PCR dye, ROX reference dye, and the EvaEZ™ polymerase activity assay.

To learn about microbial viability PCR using PMA and PMAxx™ viability dyes, visit the viability PCR technology page.

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    Cheetah™ HotStart Taq DNA Polymerase

    From: $221 Sizes: 500 UCatalog #: 29050

    Cheetah™ Taq is a chemically modified hot-start Taq DNA polymerase useful for preventing or reducing nonspecific DNA amplification in PCR. Modified using a novel modifying reagent, Cheetah™ Taq represents a major improvement upon AmpliTaq® Gold by having a faster activation time and better shelf-life.

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    500 U
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    Cheetah™ Taq is a chemically modified hot-start Taq DNA polymerase useful for preventing or reducing nonspecific DNA amplification in PCR. Modified using a novel modifying reagent, Cheetah™ Taq represents a major improvement upon AmpliTaq® Gold by having a faster activation time and better shelf-life.

     

    dNTP Mix, 10 mM Each

    From: $368 Sizes: 5 x 1 mLCatalog #: 40054

    Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 10 mM in the solution provided.

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    5 x 1 mL
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    Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 10 mM in the solution provided.

     

    dNTP Mix, 25 mM Each

    From: $189 Sizes: 1 mLCatalog #: 40053

    Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 25 mM in the solution provided.

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    1 mL
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    Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 25 mM in the solution provided.

     

    dNTP Set, 100 mM Each

    From: $189 Sizes: 1 set - 250 uL eachCatalog #: 40052

    Highly purified dNTPs (>99% by HPLC) in purified water. Each deoxynucleotide solution (dATP, dCTP, dGTP, dTTP) is provided in a separate vial at 100 mM.

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    1 set - 250 uL each
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    Highly purified dNTPs (>99% by HPLC) in purified water. Each deoxynucleotide solution (dATP, dCTP, dGTP, dTTP) is provided in a separate vial at 100 mM.

     

    EvaEZ™ Fluorometric Polymerase Activity Assay Kit

    From: $368 Sizes: 2 x 1 mLCatalog #: 29051

    EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes. It contains EvaGreen® dye together with a primed template, dNTPs, and MgCl2 in a Tris buffer system. Available without magnesium by special order.

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    2 x 1 mL
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    EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes. It contains EvaGreen® dye together with a primed template, dNTPs, and MgCl2 in a Tris buffer system. Available without magnesium by special order.

     

    EvaGreen® Dye, 2000X in DMSO

    From: $176 Sizes: 50 uLCatalog #: 31019

    EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis.

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    EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis.

     

    EvaGreen® Dye, 20X in Water

    From: $44 Sizes: Trial size (1 mL), 5 x 1 mLCatalog #: 31000, - 31000-TView allHide

    EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis.

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    EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis.

     

    Fast EvaGreen® qPCR Master Mix

    From: $66 Sizes: Trial size (1 mL), 2 x 1 mL, 5 x 1 mL, 50 x 1 mLCatalog #: 31003, 31003-1, 31003-2, - 31003-TView allHide

    Fast Evagreen® qPCR Master Mix is a PCR reagent suitable for qPCR using a fast cycling protocol. The reagent system uses two of our breakthrough PCR technologies, Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, to deliver superior results when compared to other commercial master mixes.

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    Fast Evagreen® qPCR Master Mix is a PCR reagent suitable for qPCR using a fast cycling protocol. The reagent system uses two of our breakthrough PCR technologies, Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, to deliver superior results when compared to other commercial master mixes.

     

    Fast Plus EvaGreen® qPCR Master Mix

    From: $76 Sizes: Trial size (1 mL), 2 x 1 mL, 5 x 1 mL, 50 x 1 mLCatalog #: 31014, 31015, 31020, 31014-1, 31014-2, 31014-T, 31015-1, 31015-2, 31015-T, 31020-1, 31020-2, - 31020-TView allHide

    Fast Plus EvaGreen® qPCR master mix is a ready-to-use hot-start mix for nucleic acid quantitation and melt curve analysis of PCR amplicons. Formulated using our environmentally safe EvaGreen® dye and fast-activating chemically-modified hotstart enzyme, Cheetah™ Taq, Biotium's PCR master mix delivers superior performance even with the most challenging samples tested.

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    Fast Plus EvaGreen® qPCR master mix is a ready-to-use hot-start mix for nucleic acid quantitation and melt curve analysis of PCR amplicons. Formulated using our environmentally safe EvaGreen® dye and fast-activating chemically-modified hotstart enzyme, Cheetah™ Taq, Biotium's PCR master mix delivers superior performance even with the most challenging samples tested.

     

    Fast Probe Master Mix

    From: $83 Sizes: Trial size (1 mL), 2 x 1 mL, 5 x 1 mL, 50 x 1 mLCatalog #: 31005, 31016, 31005-1, 31005-2, 31005-T, 31016-1, 31016-2, - 31016-TView allHide

    Fast Probe Master Mix is formulated for conducting real-time PCR using an oligo-based probe, such as a TaqMan® probe, a MGB probe or an AllGlo™ probe. The master mix contains buffer, dNTPs, MgCl2 and our superior hotstart enzyme Cheetah™ Taq.

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    Fast Probe Master Mix is formulated for conducting real-time PCR using an oligo-based probe, such as a TaqMan® probe, a MGB probe or an AllGlo™ probe. The master mix contains buffer, dNTPs, MgCl2 and our superior hotstart enzyme Cheetah™ Taq.

     

    Forget-Me-Not™ EvaGreen® qPCR Master Mix (2-Color Tracking)

    From: $58 Sizes: 1 mL (100 reactions) Trial Size, 5 x 1 mL (500 reactions), 2 x 10 mL (2000 reactions)Catalog #: , , 31041-T, 31041-1, 31041-20mL, 31042-T, 31042-1, - 31042-20mLView allHide

    Forget-Me-Not™ EvaGreen® is a high performance qPCR master mix with 2-color reaction component tracking system available for a fraction of the cost of similar products.

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    1 mL (100 reactions) Trial Size
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    Forget-Me-Not™ EvaGreen® is a high performance qPCR master mix with 2-color reaction component tracking system available for a fraction of the cost of similar products.

     

    Forget-Me-Not™ EvaGreen® qPCR Master Mix (Low ROX or High ROX)

    From: $60 Sizes: 1 mL (100 reactions) Trial Size, 5 x 1 mL (500 reactions), 2 x 10 mL (2000 reactions)Catalog #: , , 31045-1mL, 31045-5mL, 31045-20mL, 31046-1mL, 31046-5mL, - 31046-20mLView allHide

    Forget-Me-Not™ EvaGreen® qPCR Master Mix is a hotstart EvaGreen® dye-based master mix for use in real time PCR applications and DNA melt curve analysis.

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    1 mL (100 reactions) Trial Size
    5 x 1 mL (500 reactions)
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    Forget-Me-Not™ EvaGreen® qPCR Master Mix is a hotstart EvaGreen® dye-based master mix for use in real time PCR applications and DNA melt curve analysis.

     

    Forget-Me-Not™ Universal Probe qPCR Master Mix

    From: $60 Sizes: 1 mL (100 reactions) Trial Size, 5 x 1 mL (500 reactions)Catalog #: 31043-1, 31043-T, 31044-1, - 31044-TView allHide

    2X Forget-Me-Not™ Universal Probe qPCR Master Mix is a high-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping. This kit is suitable for all fluorescent probe-based technologies, including hydrolysis probes (such as TaqMan® and dual-labeled BHQ® probes) and displacement probes (like Molecular Beacons).

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    1 mL (100 reactions) Trial Size
    5 x 1 mL (500 reactions)
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    2X Forget-Me-Not™ Universal Probe qPCR Master Mix is a high-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping. This kit is suitable for all fluorescent probe-based technologies, including hydrolysis probes (such as TaqMan® and dual-labeled BHQ® probes) and displacement probes (like Molecular Beacons).

     

    HotStart Polymerase Modification Kit

    From: $194 Sizes: Trial size, 1 kitCatalog #: 29054, - 29054-TView allHide

    The HotStart Polymerase Modification Kit provides an easy way to reversibly modify lysine residues of thermostable DNA polymerase, rendering the enzyme inactive. The modification is reversed after heating to >90°C. HotStart modification of DNA polymerase for PCR prevents amplification of non-specific PCR products due to low stringency annealing of primers at low temperature during reaction assembly.

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    The HotStart Polymerase Modification Kit provides an easy way to reversibly modify lysine residues of thermostable DNA polymerase, rendering the enzyme inactive. The modification is reversed after heating to >90°C. HotStart modification of DNA polymerase for PCR prevents amplification of non-specific PCR products due to low stringency annealing of primers at low temperature during reaction assembly.

     

    PMA Enhancer for Gram Negative Bacteria, 5X Solution

    From: $74 Sizes: 16 mLCatalog #: 31038

    PMA Enhancer for Gram Negative Bacteria was designed to improve PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria.

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    16 mL
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    PMA Enhancer for Gram Negative Bacteria was designed to improve PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria.

     

    PMA Real-Time PCR Bacterial Viability Kit – E. coli (uidA)

    From: $315 Sizes: 200 assaysCatalog #: 31050, - 31050-XView allHide

    This kit contains primers for amplification of E. coli uidA gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx, and perform 200 PCR reactions.

    Viability dye
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    200 assays
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    This kit contains primers for amplification of E. coli uidA gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx, and perform 200 PCR reactions.