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qPCR Reagents

The Forget-Me-Not™ series of qPCR master mixes are robust, sensitive, and affordable master mixes for all of your qPCR needs. For DNA dye-based qPCR, the Forget-Me-Not™ EvaGreen® master mixes are an excellent choice, and are offered in two formats: basic, and with two-color tracking to reduce pipetting errors. For probe-based qPCR, the Forget-Me-Not™ Universal Probe master mix is ideal for all probe types, such as TaqMan® or Molecular Beacons.

We also offer PCR accessory products such as dNTPs, Cheetah™ Taq HotStart Polymerase, EvaGreen® PCR dye, ROX reference dye, and the EvaEZ™ polymerase activity assay.

To learn about microbial viability PCR using PMA and PMAxx™ viability dyes, visit the viability PCR technology page.

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    Cheetah™ HotStart Taq DNA Polymerase

    From: $239 Sizes: 500 UCatalog #: 29050

    A hot-start Taq DNA polymerase modified using a novel modifying reagent, it has a faster activation time and better shelf-life.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    500 U
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    A hot-start Taq DNA polymerase modified using a novel modifying reagent, it has a faster activation time and better shelf-life.

     

    EvaEZ™ Fluorometric Polymerase Activity Assay Kit

    From: $398 Sizes: 2 x 1 mLCatalog #: 29051

    An easy and accurate way to determine the activity of a DNA polymerase without using radioisotopes.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    2 x 1 mL
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    An easy and accurate way to determine the activity of a DNA polymerase without using radioisotopes.

     

    EvaGreen® Dye, 20X in Water

    From: $47 Sizes: Trial size (1 mL), 5 x 1 mLCatalog #: 31000, - 31000-TView allHide

    A patented green fluorescent nucleic acid dye with features that are ideal for applications such as qPCR, HRM®, LAMP, and digital PCR.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    Trial size (1 mL)
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    A patented green fluorescent nucleic acid dye with features that are ideal for applications such as qPCR, HRM®, LAMP, and digital PCR.

     

    EvaGreen® Plus Dye, 20X in Water

    From: $92 Sizes: Trial size (1 mL), 5 x 1 mLCatalog #: 31077-T, - 31077View allHide

    EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.

     

    Forget-Me-Not™ EvaGreen® qPCR Master Mix (2-Color Tracking)

    From: $63 Sizes: 1 mL (100 reactions) Trial Size, 5 x 1 mL (500 reactions), 2 x 10 mL (2000 reactions)Catalog #: 31041-T, 31041-1, 31041-20mL, 31042-T, 31042-1, - 31042-20mLView allHide

    A high-performance dye-based qPCR master mix containing EvaGreen®, Cheetah™ HotStart Taq Polymerase, and the Forget-Me-Not™ 2-color tracking system.

    Reference dye
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    1 mL (100 reactions) Trial Size
    5 x 1 mL (500 reactions)
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    A high-performance dye-based qPCR master mix containing EvaGreen®, Cheetah™ HotStart Taq Polymerase, and the Forget-Me-Not™ 2-color tracking system.

     

    Forget-Me-Not™ EvaGreen® qPCR Master Mix (Low ROX or High ROX)

    From: $63 Sizes: 1 mL (100 reactions) Trial Size, 5 x 1 mL (500 reactions), 2 x 10 mL (2000 reactions)Catalog #: 31045-1mL, 31045-5mL, 31045-20mL, 31046-1mL, 31046-5mL, - 31046-20mLView allHide

    A high-performance dye-based qPCR master mix containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. With low or high ROX.

    Reference dye
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    1 mL (100 reactions) Trial Size
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    2 x 10 mL (2000 reactions)
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    A high-performance dye-based qPCR master mix containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. With low or high ROX.

     

    Forget-Me-Not™ Universal Probe qPCR Master Mix

    From: $63 Sizes: 1 mL (100 reactions) Trial Size, 5 x 1 mL (500 reactions)Catalog #: 31043-1, 31043-T, 31044-1, - 31044-TView allHide

    A high-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping.

    Reference dye
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    1 mL (100 reactions) Trial Size
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    A high-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping.

     

    dNTP Mix, 10 mM Each

    From: $397 Sizes: 5 x 1 mLCatalog #: 40054

    Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 10 mM in the solution provided.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    5 x 1 mL
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    Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 10 mM in the solution provided.

     

    dNTP Mix, 25 mM Each

    From: $205 Sizes: 1 mLCatalog #: 40053

    Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 25 mM in the solution provided.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    1 mL
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    Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 25 mM in the solution provided.

     

    dNTP Set, 100 mM Each

    From: $205 Sizes: 1 set - 250 uL eachCatalog #: 40052

    Highly purified dNTPs (>99% by HPLC) in purified water. Each deoxynucleotide solution (dATP, dCTP, dGTP, dTTP) is provided in a separate vial at 100 mM.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    1 set - 250 uL each
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    Highly purified dNTPs (>99% by HPLC) in purified water. Each deoxynucleotide solution (dATP, dCTP, dGTP, dTTP) is provided in a separate vial at 100 mM.

     

    EvaGreen® Dye, 2000X in DMSO

    From: $189 Sizes: 50 uLCatalog #: 31019

    A patented green fluorescent nucleic acid dye with features that are ideal for applications such as qPCR, HRM®, LAMP, and digital PCR. Highly concentrated for customers with specialized applications requiring a concentrated dye solution.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    50 uL
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    A patented green fluorescent nucleic acid dye with features that are ideal for applications such as qPCR, HRM®, LAMP, and digital PCR. Highly concentrated for customers with specialized applications requiring a concentrated dye solution.

     

    Fast EvaGreen® qPCR Master Mix

    From: $71 Sizes: Trial size (1 mL), 2 x 1 mL, 5 x 1 mL, 50 x 1 mLCatalog #: 31003, 31003-1, 31003-2, - 31003-TView allHide

    A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    Trial size (1 mL)
    2 x 1 mL
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    A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.

     

    Fast Plus EvaGreen® qPCR Master Mix

    From: $81 Sizes: Trial size (1 mL), 2 x 1 mL, 5 x 1 mL, 50 x 1 mLCatalog #: 31014, 31015, 31020, 31014-1, 31014-2, 31014-T, 31015-1, 31015-2, 31015-T, 31020-1, 31020-2, - 31020-TView allHide

    A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.

    Reference dye
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    Trial size (1 mL)
    2 x 1 mL
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    A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.

     

    Fast Probe Master Mix

    From: $89 Sizes: Trial size (1 mL), 2 x 1 mL, 5 x 1 mL, 50 x 1 mLCatalog #: 31005, 31016, 31005-1, 31005-2, 31005-T, 31016-1, 31016-2, - 31016-TView allHide

    A qPCR master mix containing Cheetah™ Taq hotstart DNA polymerase, suitable for probe-based real-time PCR applications.

    Reference dye
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    Trial size (1 mL)
    2 x 1 mL
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    A qPCR master mix containing Cheetah™ Taq hotstart DNA polymerase, suitable for probe-based real-time PCR applications.

     

    HotStart Polymerase Modification Kit

    From: $210 Sizes: Trial size, 1 kitCatalog #: 29054, - 29054-TView allHide

    A simple kit for reversibly modifying thermostable DNA polymerases, rendering the enzyme inactive and preventing primer dimers during reaction set-up.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    (This product is available by special order only)
    This product is available by special order only.

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    A simple kit for reversibly modifying thermostable DNA polymerases, rendering the enzyme inactive and preventing primer dimers during reaction set-up.

     

    ROX Reference Dye, 25 uM in TE Buffer

    From: $141 Sizes: 5 x 1 mLCatalog #: 29052

    A dye used to normalize well-to-well variations that may occur during quantitative, real-time PCR reactions.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    5 x 1 mL
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    A dye used to normalize well-to-well variations that may occur during quantitative, real-time PCR reactions.