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qPCR Reagents

Showing 1–16 of 26 results

    Forget-Me-Not™ EvaGreen® qPCR Master Mix (Low ROX or High ROX)

    From: $60 Sizes: 1 mL (100 reactions) Trial Size, 5 x 1 mL (500 reactions), 2 x 10 mL (2000 reactions)Catalog #: , , 31045-1mL, 31045-5mL, 31045-20mL, 31046-1mL, 31046-5mL, - 31046-20mLView allHide

    Forget-Me-Not™ EvaGreen® qPCR Master Mix is a hotstart EvaGreen® dye-based master mix for use in real time PCR applications and DNA melt curve analysis.

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    1 mL (100 reactions) Trial Size
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    Forget-Me-Not™ EvaGreen® qPCR Master Mix is a hotstart EvaGreen® dye-based master mix for use in real time PCR applications and DNA melt curve analysis.

     

    Thiazole Green, 10,000X in DMSO

    From: $128 Sizes: 0.5 mL, 1 mLCatalog #: 40086-0.5mL, - 40086-1mLView allHide

    Thiazole Green, which is structurally identical to SYBR® Green I Nucleic Acid Gel Stain, is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose, polyacrylamide gels and qPCR.

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    Thiazole Green, which is structurally identical to SYBR® Green I Nucleic Acid Gel Stain, is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose, polyacrylamide gels and qPCR.

     

    PMA Real-Time PCR Bacterial Viability Kit – Legionella pneumophila (mip)

    From: $315 Sizes: 200 assaysCatalog #: 31053

    This kit contains primers for amplification of Legionella pneumophila mip gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.

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    200 assays
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    This kit contains primers for amplification of Legionella pneumophila mip gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.

     

    PMA Real-Time PCR Bacterial Viability Kit – Listeria monocytogenes (hly)

    From: $315 Sizes: 200 assaysCatalog #: 31051, - 31051-XView allHide

    This kit contains primers for amplification of Listeria monocytogenes hly gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions.

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    200 assays
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    This kit contains primers for amplification of Listeria monocytogenes hly gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions.

     

    PMA Real-Time PCR Bacterial Viability Kit – E. coli (uidA)

    From: $315 Sizes: 200 assaysCatalog #: 31050, - 31050-XView allHide

    This kit contains primers for amplification of E. coli uidA gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx, and perform 200 PCR reactions.

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    200 assays
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    This kit contains primers for amplification of E. coli uidA gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx, and perform 200 PCR reactions.

     

    dNTP Mix, 10 mM each

    From: $368 Sizes: 5 x 1 mLCatalog #: 40054

    Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 10 mM in the solution provided.

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    5 x 1 mL
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    Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 10 mM in the solution provided.

     

    dNTP Mix, 25 mM each

    From: $189 Sizes: 1 mLCatalog #: 40053

    Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 25 mM in the solution provided.

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    Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 25 mM in the solution provided.

     

    dNTP Set, 100 mM each

    From: $189 Sizes: 1 set - 250 uL eachCatalog #: 40052

    Highly purified dNTPs (>99% by HPLC) in purified water. Each deoxynucleotide solution (dATP, dCTP, dGTP, dTTP) is provided in a separate vial at 100 mM.

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    1 set - 250 uL each
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    Highly purified dNTPs (>99% by HPLC) in purified water. Each deoxynucleotide solution (dATP, dCTP, dGTP, dTTP) is provided in a separate vial at 100 mM.

     

    PMA Enhancer for Gram Negative Bacteria, 5X Solution

    From: $74 Sizes: 16 mLCatalog #: 31038

    PMA Enhancer for Gram Negative Bacteria was designed to improve PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria.

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    16 mL
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    PMA Enhancer for Gram Negative Bacteria was designed to improve PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria.

     

    PMA Real-Time PCR Bacterial Viability Kit – E. coli O157:H7 (Z3276)

    From: $315 Sizes: 200 assaysCatalog #: 31037, - 31037-XView allHide

    This kit contains primers for amplification of E. coli strain O157:H7 Z3276 gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions.

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    200 assays
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    This kit contains primers for amplification of E. coli strain O157:H7 Z3276 gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions.

     

    PMA Real-Time PCR Bacterial Viability Kit – Mycobacterium tuberculosis (groEL2)

    From: $315 Sizes: 200 assaysCatalog #: 31034

    This kit contains primers for amplification of Mycobacterium tuberculosis groEL2 gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.

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    200 assays
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    This kit contains primers for amplification of Mycobacterium tuberculosis groEL2 gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.

     

    PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (mecA)

    From: $315 Sizes: 200 assaysCatalog #: 31036

    This kit contains primers for amplification of the methicillin resistance gene mecA, which confers methicillin-resistance in Staphyloccoccus aureus and other bacterial species. The kit includes reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.

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    200 assays
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    This kit contains primers for amplification of the methicillin resistance gene mecA, which confers methicillin-resistance in Staphyloccoccus aureus and other bacterial species. The kit includes reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.

     

    PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (nuc)

    From: $315 Sizes: 200 assaysCatalog #: 31035

    This kit contains primers for amplification of  Staphylococcus aureus-specific nuc gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.

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    200 assays
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    This kit contains primers for amplification of  Staphylococcus aureus-specific nuc gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.

     

    PMA Real-Time PCR Bacterial Viability Kit – Salmonella enterica (invA)

    From: $315 Sizes: 200 assaysCatalog #: 31033, - 31033-XView allHide

    This kit contains primers for amplification of Salmonella enterica invA gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions.

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    200 assays
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    This kit contains primers for amplification of Salmonella enterica invA gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions.

     

    WarmStart Enzyme Modification Kit

    From: $194 Sizes: Trial size, 1 kitCatalog #: 29053, - 29053-TView allHide

    The WarmStart™ Enzyme Modification Kit allows temperature control of enzyme activity to be applied to non-thermostable enzymes like reverse transcriptase. Similar to our HotStart polymerase modification reagent (catalog no. 29054), the enzyme is inactivated by modification of lysine residues with a novel chemical modifier. While HotStart modification is reversed by heating to 90°C or higher, WarmStart™ protein activity is restored by heating to 45°C or higher.

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    The WarmStart™ Enzyme Modification Kit allows temperature control of enzyme activity to be applied to non-thermostable enzymes like reverse transcriptase. Similar to our HotStart polymerase modification reagent (catalog no. 29054), the enzyme is inactivated by modification of lysine residues with a novel chemical modifier. While HotStart modification is reversed by heating to 90°C or higher, WarmStart™ protein activity is restored by heating to 45°C or higher.

     

    EvaGreen® Dye, 2000X in DMSO

    From: $176 Sizes: 50 uLCatalog #: 31019

    EvaGreen® Dye, 2000X  in DMSO is offered for customers with specialized applications requiring a concentrated dye solution. Note: for most qPCR applications, we recommend EvaGreen® Dye, 20X  in water (see 31000 for product description), or one of our EvaGreen® qPCR Master Mixes.

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    50 uL
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    EvaGreen® Dye, 2000X  in DMSO is offered for customers with specialized applications requiring a concentrated dye solution. Note: for most qPCR applications, we recommend EvaGreen® Dye, 20X  in water (see 31000 for product description), or one of our EvaGreen® qPCR Master Mixes.