qPCR Reagents
The Forget-Me-Not™ series of qPCR master mixes are robust, sensitive, and affordable master mixes for all of your qPCR needs. For DNA dye-based qPCR, the Forget-Me-Not™ EvaGreen® master mixes are an excellent choice, and are offered in two formats: basic, and with two-color tracking to reduce pipetting errors. For probe-based qPCR, the Forget-Me-Not™ Universal Probe master mix is ideal for all probe types, such as TaqMan® or Molecular Beacons.
We also offer PCR accessory products such as dNTPs, Cheetah™ Taq HotStart Polymerase, EvaGreen® PCR dye, ROX reference dye, and the EvaEZ™ polymerase activity assay.
To learn about microbial viability PCR using PMA and PMAxx™ viability dyes, visit the viability PCR technology page.
Cheetah™ HotStart Taq DNA Polymerase
Cheetah™ Taq is a chemically modified hot-start Taq DNA polymerase useful for preventing or reducing nonspecific DNA amplification in PCR. Modified using a novel modifying reagent, Cheetah™ Taq represents a major improvement upon AmpliTaq® Gold by having a faster activation time and better shelf-life.
Cheetah™ Taq is a chemically modified hot-start Taq DNA polymerase useful for preventing or reducing nonspecific DNA amplification in PCR. Modified using a novel modifying reagent, Cheetah™ Taq represents a major improvement upon AmpliTaq® Gold by having a faster activation time and better shelf-life.
EvaEZ™ Fluorometric Polymerase Activity Assay Kit
EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes. It contains EvaGreen® dye together with a primed template, dNTPs, and MgCl2 in a Tris buffer system. Available without magnesium by special order.
EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes. It contains EvaGreen® dye together with a primed template, dNTPs, and MgCl2 in a Tris buffer system. Available without magnesium by special order.
EvaGreen® Dye, 20X in Water
EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis.
EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis.
Forget-Me-Not™ EvaGreen® qPCR Master Mix (2-Color Tracking)
Forget-Me-Not™ EvaGreen® is a high performance qPCR master mix with 2-color reaction component tracking system available for a fraction of the cost of similar products.
Forget-Me-Not™ EvaGreen® is a high performance qPCR master mix with 2-color reaction component tracking system available for a fraction of the cost of similar products.
Forget-Me-Not™ EvaGreen® qPCR Master Mix (Low ROX or High ROX)
Forget-Me-Not™ EvaGreen® qPCR Master Mix is a hotstart EvaGreen® dye-based master mix for use in real time PCR applications and DNA melt curve analysis.
Forget-Me-Not™ EvaGreen® qPCR Master Mix is a hotstart EvaGreen® dye-based master mix for use in real time PCR applications and DNA melt curve analysis.
Forget-Me-Not™ Universal Probe qPCR Master Mix
2X Forget-Me-Not™ Universal Probe qPCR Master Mix is a high-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping. This kit is suitable for all fluorescent probe-based technologies, including hydrolysis probes (such as TaqMan® and dual-labeled BHQ® probes) and displacement probes (like Molecular Beacons).
2X Forget-Me-Not™ Universal Probe qPCR Master Mix is a high-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping. This kit is suitable for all fluorescent probe-based technologies, including hydrolysis probes (such as TaqMan® and dual-labeled BHQ® probes) and displacement probes (like Molecular Beacons).
PMA Enhancer for Gram Negative Bacteria, 5X Solution
PMA Enhancer for Gram Negative Bacteria was designed to improve PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria.
PMA Enhancer for Gram Negative Bacteria was designed to improve PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria.
PMA Real-Time PCR Bacterial Viability Kit – E. coli (uidA)
This kit contains primers for amplification of E. coli uidA gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx, and perform 200 PCR reactions.
This kit contains primers for amplification of E. coli uidA gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx, and perform 200 PCR reactions.
PMA Real-Time PCR Bacterial Viability Kit – E. coli O157:H7 (Z3276)
This kit contains primers for amplification of E. coli strain O157:H7 Z3276 gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions.
This kit contains primers for amplification of E. coli strain O157:H7 Z3276 gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions.
PMA Real-Time PCR Bacterial Viability Kit – Legionella pneumophila (mip)
This kit contains primers for amplification of Legionella pneumophila mip gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.
This kit contains primers for amplification of Legionella pneumophila mip gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.
PMA Real-Time PCR Bacterial Viability Kit – Listeria monocytogenes (hly)
This kit contains primers for amplification of Listeria monocytogenes hly gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions.
This kit contains primers for amplification of Listeria monocytogenes hly gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions.
PMA Real-Time PCR Bacterial Viability Kit – Mycobacterium tuberculosis (groEL2)
This kit contains primers for amplification of Mycobacterium tuberculosis groEL2 gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.
This kit contains primers for amplification of Mycobacterium tuberculosis groEL2 gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.
PMA Real-Time PCR Bacterial Viability Kit – Salmonella enterica (invA)
This kit contains primers for amplification of Salmonella enterica invA gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions.
This kit contains primers for amplification of Salmonella enterica invA gene, with reagents sufficient to treat 80 bacterial cultures with PMA or PMAxx™, and perform 200 PCR reactions.
PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (mecA)
This kit contains primers for amplification of the methicillin resistance gene mecA, which confers methicillin-resistance in Staphyloccoccus aureus and other bacterial species. The kit includes reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.
This kit contains primers for amplification of the methicillin resistance gene mecA, which confers methicillin-resistance in Staphyloccoccus aureus and other bacterial species. The kit includes reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.
PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (nuc)
This kit contains primers for amplification of Staphylococcus aureus-specific nuc gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.
This kit contains primers for amplification of Staphylococcus aureus-specific nuc gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions.
dNTP Mix, 10 mM Each
Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 10 mM in the solution provided.
Highly purified mix of dNTPs (>99% by HPLC) in 0.6 mM Tris-HCl (pH 7.5); each dNTP is at a concentration of 10 mM in the solution provided.