Steady-Luc Firefly HTS Assay Kit

Steady-Luc Firefly HTS Assay Kit
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Catalog #: 30028-1, 30028-2, 30028-3, 30028-T
 Multiple sizes
Categories: , , , .

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Product Description

Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the lack of endogenous activity in mammalian cells or tissues. However, the light production resulting from the reaction leads to formation of suicidal adenyl-oxyluciferin at the enzyme surface. It results in very short half-life of the light emission with a flash-type kinetics. Because of this, flash-type luminescence must be detected immediately after initiating the reaction, which is not compatible with batch analysis of samples.

Biotium’s Steady-Luc™ HTS assay system is a proprietary mixture of substances that modify the enzymatic reaction to produce a long lasting signal (steady glow) by preventing the formation of adenyl-oxyluciferin at the enzyme surface. It is a homogeneous high sensitivity firefly luciferase reporter gene assay kit with a half-life of about 3 hours for the quantification of firefly luciferase expression in mammalian cells, allowing batch processing in microplates.

Glow-type luciferase assays like Steady-Luc™ have lower luminescence signal compared to flash-type assays. The sensitivity and limit of detection of the assay will depend on luciferase expression levels in your experimental system as well as luminometer sensitivity.

Biotium also offers Steady-Luc™ Firefly HTS Assay Kit (Lyophilized) (catalog no. 30028L), a new packaging format that includes lyophilized assay buffer for convenient room temperature shipping and storage at -20°C.

Kit Components:

  • D-Luciferin
  • Steady-Luc Firefly Assay Buffer


  • Simple: single step homogeneous assay.
  • Glow kinetics: luminescence half life of about 3 hours.
  • Economic: save up to 45% compared to other brands.



Additional Information


40 assays, 120 assays, 1,000 assays, 10,000 assays

MSDS (PDF): MSDS 30028

Protocol (PDF): PI-30028, 30028L

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.