Reagents for Nitric Oxide (NO) & Reactive Oxygen Species (ROS)
Dyes and probes for detecting Nitric Oxide (NO) or reactive oxygen in cells.
Griess Reagent Kit
Griess reagent is used to detect nitrite photometrically. The kit includes ready-to-use Griess reagent, nitrite standard solution, and a detailed protocol.
Griess reagent is used to detect nitrite photometrically. The kit includes ready-to-use Griess reagent, nitrite standard solution, and a detailed protocol.
2,3-Diaminonaphthalene
2,3-Diaminonaphthalene reacts with nitrosonium, which is formed from NO, to form the fluorescent dye 1 H-naphthotriazole. This method can be used to detect 10 nM to 10 uM of nitrite (NO2-) and is compatible with 96-well format.
2,3-Diaminonaphthalene reacts with nitrosonium, which is formed from NO, to form the fluorescent dye 1 H-naphthotriazole. This method can be used to detect 10 nM to 10 uM of nitrite (NO2-) and is compatible with 96-well format.
7-Nitroindazole (7-NI)
7-NI is a selective inhibitor for the brain nitric oxide synthase (NOS). Also see the NOS inhibitor kit (catalog no. 00249).
7-NI is a selective inhibitor for the brain nitric oxide synthase (NOS). Also see the NOS inhibitor kit (catalog no. 00249).
DAA
DAA (1,2-Diaminoanthraquinone) reacts with nitric oxide (NO) to form a red (λ Em 580 nm) fluorescent precipitate. DAA has been used to detect NO concentration in rat retinas after injury to the optic nerve.
DAA (1,2-Diaminoanthraquinone) reacts with nitric oxide (NO) to form a red (λ Em 580 nm) fluorescent precipitate. DAA has been used to detect NO concentration in rat retinas after injury to the optic nerve.
DEA-NONOate
DEA-NONOate (2-(N,N-Diethylamino)-diazenolate-2-oxide, diethylammonium salt) releases nitric oxide spontaneously under physiological conditions. Useful for reliable NO generation in solution.
DEA-NONOate (2-(N,N-Diethylamino)-diazenolate-2-oxide, diethylammonium salt) releases nitric oxide spontaneously under physiological conditions. Useful for reliable NO generation in solution.
Dihydroethidium (Hydroethidium)
Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes.
Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes.
Dihydrorhodamine 123
Dihydrorhodamine 123 is the reduced form of rhodamine 123 (70010), which is a commonly used fluorescent mitochondrial dye.
Dihydrorhodamine 123 is the reduced form of rhodamine 123 (70010), which is a commonly used fluorescent mitochondrial dye.
Dihydrorhodamine 123, Dihydrochloride Salt
Dihydrorhodamine 123 dihydrochloride is functionally equivalent to dihydrorhodamine 123 (10055) but with increased stability toward air oxidation and light during storage.
Dihydrorhodamine 123 dihydrochloride is functionally equivalent to dihydrorhodamine 123 (10055) but with increased stability toward air oxidation and light during storage.
H2DCFDA
H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H2DCFDA becomes the highly green fluorescent 2′,7′-dichlorofluorescein.
H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H2DCFDA becomes the highly green fluorescent 2′,7′-dichlorofluorescein.
L-NIL
L-NIL (N6-(1-Iminoethyl)-lysine, hydrochloride) is a potent and selective inhibitor of nitric oxide synthase that exhibits about 28-fold greater selectivity for inducible nitric oxide synthase (IC50 = 3.3 uM) than for the rat brain constitutive enzyme (IC50 = 92 uM).
L-NIL (N6-(1-Iminoethyl)-lysine, hydrochloride) is a potent and selective inhibitor of nitric oxide synthase that exhibits about 28-fold greater selectivity for inducible nitric oxide synthase (IC50 = 3.3 uM) than for the rat brain constitutive enzyme (IC50 = 92 uM).
L-NIO
L-NIO (N5-(1-Iminoethyl)-L-ornithine, dihydrochloride) is a potent, irreversible inhibitor of neutrophil nitric oxide synthase. Also see our NOS inhibitor kit (00249).
L-NIO (N5-(1-Iminoethyl)-L-ornithine, dihydrochloride) is a potent, irreversible inhibitor of neutrophil nitric oxide synthase. Also see our NOS inhibitor kit (00249).
L-NMMA
L-NMMA (NG-Methyl-L-arginine, acetate salt) competitively inhibits nitric oxide synthase in a wide range of cells. Also see our NOS inhibitor kit (00249).
L-NMMA (NG-Methyl-L-arginine, acetate salt) competitively inhibits nitric oxide synthase in a wide range of cells. Also see our NOS inhibitor kit (00249).
Methyl Coelenterazine
Methyl coelenterazine (Coelenterazine, 2-methyl analog) has been reported to be a superior antioxidant for cells against reactive oxygen species (ROS) such as singlet oxygen and superoxide anion (1).
Methyl coelenterazine (Coelenterazine, 2-methyl analog) has been reported to be a superior antioxidant for cells against reactive oxygen species (ROS) such as singlet oxygen and superoxide anion (1).
Nitric Oxide Generation Kit
This kit contains 10 mg each of the following nitric oxide donors: SIN-1 (00221), SNAP (00222), S-nitrosoglutathione (00223), spermine NONOate (00224), and DEA-NONOate (00225).
This kit contains 10 mg each of the following nitric oxide donors: SIN-1 (00221), SNAP (00222), S-nitrosoglutathione (00223), spermine NONOate (00224), and DEA-NONOate (00225).
NOS Inhibitor Kit
This kit contains four nitric oxide synthase (NOS) inhibitors: 7-NI (00240) (50 mg), L-NMMA (00241) (25 mg), L-NIL (00242) (5 mg) and L-NIO (00243) (5 mg).
This kit contains four nitric oxide synthase (NOS) inhibitors: 7-NI (00240) (50 mg), L-NMMA (00241) (25 mg), L-NIL (00242) (5 mg) and L-NIO (00243) (5 mg).
S-Nitroso-L-glutathione
Similar to SNAP, S-nitrosogluthathione releases nitric oxide spontaneously under physiological conditions.
Similar to SNAP, S-nitrosogluthathione releases nitric oxide spontaneously under physiological conditions.