Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.
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Fluo-4 AM Ester
Membrane-permeant form of Fluo-4 calcium indicator. Fluo-4 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to Fluo-4 by endogenous esterases once inside cells.
EDC (or EDAC) (1-(3-Dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride)) has recently been found to be very useful for fixing in situ chelators, including the fluorescent ion indicators for histological studies following physiological experiments. EDC is also a widely used reagent to activate carboxy groups for amine coupling.
4-Bromo A-23187, Free Acid
Similar to A-23187, 4-Bromo A-23187 is used for in situ calibrations of fluorescent Ca2+ indicators to equilibrate intracellular and extracellular calcium concentrations and to allow Mn2+ to enter cells to quench indicator fluorescence.
Calcium Calibration Buffer Kit
This kit provides a range of calibration buffers with accurate calcium concentrations and is useful for the calibration of fluorescent Ca2 indicators (1,2). This product contains 50 mL each of components A and B.
BAPTA, AM Ester
BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium.