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Firefly Luciferase Assay Kit (Lyophilized)

The Firefly Luciferase Assay Kit is designed for detection and quantification of firefly luciferase reporter enzyme from cultured cells in a simple, efficient and linear fashion.

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Product Description

Firefly luciferase is widely used as a reporter gene for studying gene regulation and function, and for pharmaceutical screening (1). It is a very sensitive genetic reporter due to the lack of any endogenous luciferase activity in mammalian cells or tissues and the very nature of bioluminescence (2). Firefly luciferase is a 62,000 dalton protein which is active as a monomer and does not require subsequent processing for its activity. The enzyme catalyzes ATP-dependent D-luciferin oxidation by oxygen into oxyluciferin with emission of light centered on 560 nm. As with many enzymes, firefly luciferase follows Michaelis-Menten kinetics, and as a result maximum light output is not achieved until the substrates and co-factor are present in large excess. When assayed under these conditions, light emitted from the reaction is directly proportional to the number of luciferase enzyme molecules. The Firefly Luciferase Assay Kit is designed for detection and quantification of firefly luciferase reporter enzyme from cultured cells in a simple, efficient and linear fashion.

Firefly Luciferase Assay Kit (Lyophilized) performs comparably to Biotium’s Firefly Luciferase Assay Kit 2.0, but includes assay buffer in lyophilized format for more economical shipping and convenient storage at -20°C.

Note: This is a flash-type assay that requires luminescence measurement immediately after adding reagents. It requires a single tube luminometer or luminescence microplate reader with reagent injectors.

Kit Components:

  • D-luciferin
  • 5X Firefly luciferase lysis buffer
  • Firefly luciferase assay buffer (lyophilized)

Features:

  • Sensitivity and Linearity: Linear correlation between firefly luciferase enzyme levels and light output
  • Convenience: Lyophilized buffer for economical shipping and convenient storage

 

More Luciferase Kits

Catalog numberProductDescription
30085Firefly Luciferase Assay Kit 2.0Flash-type firefly luciferase assay kit.
30075Firefly Luciferase Assay Kit (Lyophilized)Firefly luciferase assay kit with lyophilized assay buffer for economical room temperature shipping and convenient storage at -20°C.
999235X Firefly Lysis BufferExtra lysis buffer for use with Firefly Luciferase Assay Kits, only required if using well sizes larger than 24-well.
30028Steady-Luc™ Firefly HTS Assay KitGlow-type firefly luciferase assay with signal half-life of about 3 hours for high-throughput screening in multi-well plates.
30028LSteady-Luc Firefly HTS Assay Kit (Lyophilized)Steady-Luc kit with lyophilized assay buffer for economical room temperature shipping and convenient storage at -20°C.
30082Renilla Luciferase Assay Kit 2.0Flash-type Renilla luciferase assay kit.
999125X Passive Lysis BufferExtra lysis buffer for use with Renilla Luciferase Assay Kits, only required if using well sizes larger than 24-well.
30081Firefly and Renilla Luciferase Single Tube Assay KitAssay kit for measuring firefly and Renilla activities sequentially in the same sample in a single tube.
99821-100mL1X Passive Lysis Buffer 2.0 Extra lysis buffer for use with Firefly & Renilla Single Tube Assay Kits, only required if using well sizes larger than 24-well.
30020ATP-Glo™ Bioluminometric Cell Viability Assay KitFlash-type luminescence-based assay for measuring cell viability or ATP concentration.
22003Mini Cell ScrapersMini-cell scrapers, useful for lysing cells in 96-well, 48-well, or 24-well plates.

 

References

1. Anal. Biochem. 219:169-181 (1994).

2. Anal. Biochem. 175:5-13 (1988).

 

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