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AccuBlue® Broad Range RNA Quantitation Kit

This RNA quantitation kit boasts the widest linear range (5 ng–1000 ng RNA) of available RNA quantitation kits, while providing exceptional accuracy, sensitivity, and high RNA selectivity. A mammalian RNA standard is included for accurate quantitation of mammalian cell RNA.

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Product Description

The AccuBlue® Broad Range RNA Quantitation Kit boasts the widest linear range (5 ng–1000 ng RNA) of available RNA quantitation kits, while providing exceptional accuracy, sensitivity, and high RNA selectivity. A mammalian RNA standard is included for accurate quantitation of mammalian cell RNA. Replacement vials of RNA standard (cat # 99850) can be purchased separately in case the RNA standard in your kit becomes degraded or otherwise compromised.

Assay range

The assay is linear between 5 and 1000 ng of RNA per 200 uL assay well, which spans both Qubit® HS and Qubit® BR ranges. It is ideal for use in quantifying RNA for sensitive applications such as Next-Gen Sequencing (NGS) or reverse transcription PCR (RT-PCR).

RNA specificity

Unlike absorbance-based measurements, RNA Broad Range Dye is selective for RNA over double-stranded DNA, and can tolerate an equimolar amount of dsDNA in the sample without significant effect on RNA quantitation (purified RNA samples are still recommended).

Instrument compatibility

The AccuBlue® Broad Range RNA Quantitation assay is designed for use with fluorescence microplate readers equipped with excitation and emission filters for detecting far-red fluorescence. It is also optimized for use in the Qubit® fluorometer, using the pre-programmed RNA Broad Range program.

Features:

  • Optimized for use on fluorescent microplate readers with excitation/emission of ~ 630/670 nm
  • Optimized for use on Qubit® fluorometers in the RNA Broad Range assay
  • Wide linear range of 5-1000 ng RNA (spanning those of both the Quant-iT™ RNA and Quant-iT™ RNA Broad Range kits from Thermo Fisher Scientific)
  • Highly selective for RNA over dsDNA, therefore no DNase step is necessary
  • The mammalian RNA standard allows the most accurate quantitation of mammalian cell RNA

Kit components:

  • RNA Broad Range Dye, 200X
  • RNA Broad Range Buffer
  • RNA Dilution Buffer (can use as Standard 1 on Qubit®)
  • RNA Broad Range Standard, 100 ng/uL (can use as Standard 2 on Qubit®)
Linearity of AccuBlue® Broad Range RNA Quantitation assay between 5 and 1000 ng of RNA per well in microplate assay with excitation/emission at 630/670 nm.
The AccuBlue® Broad Range RNA quantitation assay is highly specific for RNA over DNA. Over the assay range of 5 to 1000 ng of RNA, the presence of an equal amount of DNA in the sample has a negligible effect.
 

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