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AccuBlue® Broad Range RNA Quantitation Kit

An RNA-selective, fluorescent RNA quantitation kit with the widest linear range (5 ng–1000 ng RNA) of any RNA quantitation assay. Designed for use in a microplate reader or a Qubit® fluorometer.

Product Attributes

Shipping condition

Ice pack

Storage Conditions

Store at 2 to 8 °C

Excitation/Emission

650/670 nm

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Trial size (200 assays)
1,000 assays
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Product Description

The AccuBlue® Broad Range RNA Quantitation Kit boasts the widest linear range (5 ng–1000 ng RNA) of available RNA quantitation kits, while providing exceptional accuracy, sensitivity, and high RNA selectivity. A mammalian RNA standard is included for accurate quantitation of mammalian cell RNA.

Features

  • Linear range (5-1000 ng RNA) spans Qubit® HS & BR ranges
  • Highly selective for RNA over dsDNA
  • Far-red fluorescence emission
  • For fluorescence microplate reader or Qubit® fluorometer (RNA Broad Range program)
  • Mammalian RNA standard to accurately quantify similar samples

Kit Components

  • RNA Broad Range Dye, 200X
  • RNA Broad Range Buffer
  • RNA Dilution Buffer (can use as Standard 1 on Qubit®)
  • RNA Broad Range Standard, 100 ng/uL* (can use as Standard 2 on Qubit®)

*Replacement vials of RNA standard (cat # 99850) can be purchased separately in case the RNA standard in your kit becomes degraded or otherwise compromised.

Assay range

The assay is linear between 5 and 1000 ng of RNA per 200 uL assay well, which spans both Qubit® HS and Qubit® BR ranges. It is ideal for use in quantifying RNA for sensitive applications such as Next-Gen Sequencing (NGS) or reverse transcription PCR (RT-PCR).

RNA specificity

Unlike absorbance-based measurements, RNA Broad Range Dye is selective for RNA over double-stranded DNA, and can tolerate an equimolar amount of dsDNA in the sample without significant effect on RNA quantitation (purified RNA samples are still recommended).

Instrument compatibility

The AccuBlue® Broad Range RNA Quantitation assay is designed for use with fluorescence microplate readers equipped with excitation and emission filters for detecting far-red fluorescence. It is also optimized for use in the Qubit® fluorometer, using the pre-programmed RNA Broad Range program.

Linearity of AccuBlue® Broad Range RNA Quantitation assay between 5 and 1000 ng of RNA per well in microplate assay with excitation/emission at 630/670 nm.
The AccuBlue® Broad Range RNA quantitation assay is highly specific for RNA over DNA. Over the assay range of 5 to 1000 ng of RNA, the presence of an equal amount of DNA in the sample has a negligible effect.
AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit and Quant-iT are registered trademarks of Thermo Fisher Scientific.
 

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