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PMAxx™ Dye, 20 mM in H2O

Viability PCR dye, a new and improved version of the popular viability dye propidium monoazide (PMA).

Product Attributes

Viability dye

PMAxx™

Storage Conditions

Store at -10 to -35 °C

Excitation/Emission

530/622 nm (with DNA)

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

PMAxx™ is a photoreactive DNA-binding dye for viability PCR. It was designed by Biotium scientists to be a superior alternative to the popular viability dye propidium monoazide (PMA).

  • Selectively detect viable cells using qPCR
  • Better live/dead discrimination of bacteria than PMA
  • Dead cell specific dye, binds to DNA
  • Covalently attaches to DNA after photoactivation
  • 20 mM in water, 100 uL volume

To learn more about the advantages of determining microbial or cell viability using viability PCR, visit the Viability PCR Technology Page.

About PMAxx™

PMAxx™ dye is a DNA modifier used for viability PCR, invented by scientists at Biotium. PMAxx™ is a new and improved version of our popular viability dye PMA (propidium monoazide). Like PMA, PMAxx™ is a photo-reactive dye that binds to DNA with high affinity. Upon photolysis with visible light, PMAxx™ dye becomes covalently attached to DNA. This modified DNA cannot be amplified by PCR. PMAxx™ dye is designed to be cell membrane-impermeable. Thus, in a population of live and dead cells, only dead cells are susceptible to DNA modification due to compromised cell membranes. This unique feature makes PMAxx™ highly useful in selective detection of live bacteria by qPCR.

PMAxx™ was designed by Biotium scientists to be a superior alternative to PMA, and is compatible with all existing PMA protocols, but with superior activity. While PMA is generally effective at differentiating between live and dead bacteria by qPCR, it does not completely eliminate PCR products from dead cell DNA. This could potentially give false positive results. Biotium’s new dye PMAxx™ is much more effective at eliminating PCR amplification of dead cell DNA, and therefore provides the best discrimination between live and dead bacteria.

PMAxx technology is covered by granted and/or pending US and international patents.

References

1. Front Microbiol (2016) Dec 22; 7: 2066 doi: 10.3389/fmicb.2016.02066
2. Int J Food Microbiol (2016) Jul 16; 229: 1-6 doi:10.1016/j.ijfoodmicro.2016.04.010
3. J Appl Microbiol (2017) Apr;124(4):958-964 doi:10.1111/jam.13519
4. Microbiome (2017) 5, 86 doi:10.1186/s40168-017-0285-3
5. Int J Food Microbiol (2018) 269, 64-74 doi:10.1016/j.ijfoodmicro.2018.01.015
6. PLoS One (2018) 13, e0196525 doi:10.1371/journal.pone.0196525
7. J Environ Qual (2018) 47, 1139-1145 doi:10.2134/jeq2017.12.0485
8. Front Microbiol (2018) 9, 1973 doi:10.3389/fmicb.2018.01973
9. Int J Food Microbiol (2018) 266, 1-7 doi:10.1016/j.ijfoodmicro.2017.11.011
10. Food Environ Virol (2019) 11, pp350–363 doi:10.1007/s12560-019-09392-2
11. Parasitol Res (2019) 118, 999-1010 doi:10.3389/fmicb.2018.01973

Find a list of PMA and PMAxx™ references and a list of validated bacterial strains under Supporting Documents.

 

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