Practical advice and lab tips from Biotium scientists on using fluorescence in your research
A collection of protocols for immunofluorescence, western blotting, protein labeling, cell staining, and more.
Tech tip: Imaging Bacteria Using Agarose Pads
Bacteria can be difficult to image because they are small, usually non-adherent, and often motile. Here we describe how to immobilize live or fixed bacteria using agarose pads for multi-color imaging experiments.
Tech Tip: Battling Tissue Autofluorescence
Autofluorescence in tissues can make it all but impossible to distinguish specific fluorescence staining from non-specific background. See our protocols for quenching autofluorescence with TrueBlack®.