Practical advice and lab tips from Biotium scientists on using fluorescence in your research
Tech Tip: Exosome Isolation and Staining Protocols
In this tech tip, Biotium scientists provide detailed protocols for isolating exosomes from cell supernatants using PEG precipitation or size exclusion chromatography (SEC), as well as procedures for staining purified or bead-bound exosomes for analysis by flow cytometry.
A collection of protocols for immunofluorescence, western blotting, protein labeling, and more.
Tech Tip: Imaging Bacteria Using Agarose Pads
Bacteria can be difficult to image because they are small, usually non-adherent, and often motile. Here we describe how to immobilize live or fixed bacteria using agarose pads for multi-color imaging experiments.
Tech Tip: Battling Tissue Autofluorescence
Autofluorescence in tissues can make it all but impossible to distinguish specific fluorescence staining from non-specific background. See our protocols for quenching autofluorescence with TrueBlack®.