MOLECULAR STUDIES AND DIAGNOSTICS
GelRed® is our widely-known nucleic acid stain that offers superior safety and sensitivity than than EtBr, SYBR® Safe, and other gel stains. Recent studies have demonstrated GelRed® as an effective DNA stain for RT-PCR detection of COVID-19.
- Superior sensitivity: Much more sensitive than EtBr and SYBR® Safe.
- Direct EtBr replacement: No need to change equipment or optical settings
- Safer than EtBr: Non-mutagenic and non-hazardous for disposal
- Versatile: Compatible with downstream gel purification, restriction digest, sequencing, and cloning
- Convenient: Your choice of formats and workflows (GelRed® agarose, GelRed® loading buffer, prestaining, pre-cast, or post-staining)
GelRed® is validated for RT-PCR detection of SARS-CoV-2:
EvaGreen® & EvaGreen® Plus are industry-leading DNA binding dyes that are ideal for qPCR, digital PCR, LAMP, and HRM analysis. Recent studies have implemented EvaGreen® dye in point-of-care LAMP-based diagnostic assays for COVID-19 (El-Tholoth et. al, Sun et. al).
- Superior performance: Brighter and less inhibitory than SYBR® Green I for qPCR and isothermal amplification (such as LAMP)
- Versatile: Suitable for droplet digital PCR (ddPCR), microfluidic digital PCR systems, capillary gel electrophoresis, and more
- High-resolution melt analysis: The only dye that allows you to perform qPCR and HRM analysis in the same reaction
- Safe: Non-mutagenic and non-cytotoxic
EvaGreen® is validated in numerous publications for detection of SARS-CoV-2:
Forget-Me-Not™ qPCR Master Mixes feature high-performance EvaGreen® Dye and Cheetah™ HotStart Taq DNA Polymerase as well as two-color tracking for convenient and efficient workflows.
- Ready-to-use: Just add primers and DNA template
- Optimized: Featuring our superior EvaGreen® Dye and formulated with Cheetah™ HotStart Taq for fast cycling protocols
- Convenient: 2-color tracking to prevent waste and other costly mistakes
- Available with no ROX, high or low ROX, for use with any PCR instrument
We also offer Forget-Me-Not™ Universal Probe qPCR Master Mix for high performance probe-based PCR applications such as TaqMan® and dual-labeled BHQ® probe assays.
N-Flux™ 5X Digital PCR Master Mix is a high-performance, dye-free master mix specially formulated for chip-based digital PCR (cdPCR) platforms.
- Robust results: Sensitive and reproducible performance in dPCR and qPCR assays
- Optimal sample partitioning: Minimizes interactions with hydrophobic plastic surfaces and maximizes end-point fluorescence
- Compatible with probe-based technologies: Including TaqMan® dual-labeled such as BHQ® probes, and displacement probes
- Convenient 5X concentration: Facilitates workflows for samples that are too dilute for 2X master mixes
Molecular biology applications such as NGS require sensitive and selective quantitation. AccuBlue®, AccuGreen®, and AccuClear® are fluorescent quantitation kits with industry-leading sensitivity, accuracy, and versatility.
- Quality: Accurate and sensitive detection ideal for NGS and other applications
- Ultrasensitive: Kit options for detecting down to 1 pg DNA or 5 ng RNA
- Versatile: Options for fluorescent plate reader or Qubit® fluorometer
- Selective: All kits have high selectivity for DNA or RNA
- Best Value: Economical kits with exceptional quality
Viability PCR is a powerful technology for the sensitive and rapid detection of viable microorganisms. Unlike time-consuming culturing methods, qPCR is a fast and sensitive method of detection. PMA and PMAxx™ have been validated in a wide variety of viruses, as well as yeast and bacteria (see PMA & PMAxx™ References).
- Specific: Obtain accurate viability information for targeted microorganisms in mixed cultures through primer-based specificity
- Rapid and Sensitive: Convenient PCR-based workflows for fast and ultrasensitive quantification
- Validated: v-PCR with PMA has been cited in hundreds of publications and several viruses including Hepatitis A, Norovirus, and enteroviruses
The RNAstorm™ RNA Isolation Kit allows purification of high quality total RNA from either cultured cells or fresh tissue in as little as 20 minutes. High yields (up to 120 μg) can be obtained using a simple and quick column-based protocol. Contaminating DNA is removed using a DNase treatment step, and the protocol avoids toxic chemicals such as phenol or chloroform.
- Quality: High quality total RNA suitable for next-generation sequencing (RNA-Seq), RT-PCR, cDNA synthesis, and microarrays
- Convenient: Simple 20 minute workflow yielding up to 120 μg of RNA
- DNase treatment included
- No phenol-chloroform and no ethanol precipitation required
- Cheetah™ HotStart Taq DNA Polymerase: Chemically modified hot-start Taq DNA polymerase for reducing nonspecific DNA amplification in PCR
- Labeled Nucleotides: Featuring CF® Dye labeled nucleotides, other fluorescent nucleotides, biotinylated nucleotides, and more
- Thiazole Green: A nucleic acid dye that is structurally identical to SYBR® Green I for agarose gels, polyacrylamide gels, and qPCR
- dNTP Mixes: Available in 10 mM, 25 mM, and 100 mM concentrations
- ROX reference dye: Available as a 25 uM solution in TE buffer for qPCR normalization
As COVID-19 becomes more widespread, development of immunoassays becomes critical for broad serological testing of individuals who carry antibodies against SARS-CoV-2. These assays rely on accurate detection of human IgG or IgM antibodies that bind to viral antigens. We offer several high-quality secondary antibodies, including anti-human IgG, IgM, and IgA secondary antibodies suitable for fluorescent or enzyme-based detection methods. We also have a collection of over 1000 monoclonal primary antibodies that can be used to detect and characterize induced cytokines, inflammation, and other immunological responses associated with COVID-19 infection.
Biotium offers a wide selection of highly cross-adsorbed secondary antibodies conjugated to fluorescent CF® Dyes, enzymes and fluorescent proteins.
- Highly cross-adsorbed: Minimal cross-reactivity with other target species for multiplex staining
- Large choice of labels: 20 bright and photostable CF® Dyes, HRP, AP, R-PE, APC, or biotin
- Versatile: Antibodies suitable for ELISA, immunofluorescence, immunohistology, and flow cytometry
- Custom conjugations: Custom orders for other CF® Dye labels are available upon request. Please contact technical support for inquiries.
Biotium has a growing collection of over 1000 monoclonal antibodies validated for IHC, flow cytometry, and other applications.
- Validated in IHC and other applications
- Large choice of labels: 13 bright and photostable CF® Dyes, R-PE, APC, PerCP, HRP, AP, or biotin
- Size options: 100 uL to 500 uL sizes available
- Also available unlabeled, BSA-free (ready-to-use for Mix-n-Stain™ labeling)
Mix-n-Stain™ Antibody Labeling Kits are particularly useful if custom labeling or specific antibody conjugates aren’t readily available. These kits provide efficient and versatile antibody or protein labeling in as little as 30 minutes with minimal hands-on time.
- Patented buffer system for optimal labeling and brighter conjugates
- Versatile: Labeling tolerates BSA, Tris, gelatin, trehalose, azide, and ascites fluid
- Flexible: Kit options for labeling < 5 ug antibody and up to 1 mg
- Efficient: 100% antibody recovery, no purification required.
- Choice: Label with ~30 CF® Dyes, HRP, AP, R-PE, APC, PerCP, and biotin
Biotium also offers Mix-n-Stain™ Nanobody Labeling Kits specifically designed for optimal labeling of single-domain Nanobodies® (also called camelid single variable or VHH domains).
Biotium offers many reagents for analyzing cellular viability and proliferation by flow cytometry. Our Live-or-Dye™ kits contain fixable dead cell stains available in 12 bright colors. Our ViaFluor® dyes are cell-permeable, non-toxic dyes that are useful for long-term cell labeling and cell division tracking. The novel NucView® Caspase-3 Substrates are the only caspase substrates for real-time apoptosis detection.
- Live-or-Dye™ Fixable Dead Cell Stains: Dead cell specific, amine-reactive dyes available in 12 bright and photostable colors.
- ViaFluor® Cell Proliferation Kit: Cell-permeable, non-toxic dyes for long-term cell tracking studies, available in blue and green.
- NucView® Caspase-3 Substrates: Non-toxic caspase substrates for real-time apoptosis detection by flow cytometry or microscopy, in 3 color options.
Vaccine and Antibody Characterization
Research and development towards COVID-19 vaccines and therapeutics is moving at an incredible pace, indicative of an unprecedented global research effort in both scale and speed. Several preclinical and Phase I drug candidates are either therapeutic antibodies or recombinant protein-based vaccines which require rigorous characterization prior to testing in in vivo models. Biotium continues to support this pipeline by offering several high-quality and cost efficient reagents for expression analysis and characterization of recombinant proteins.
Our One-Step stains are efficient, economical, and non-toxic protein gel stains. Staining is robust and rapid, requiring as little as 5 minutes in a single step without fixation or washing. One-Step stains are compatible with downstream applications including Edman-based sequencing and mass spectrometry.
- One-Step Blue®: A visible blue stain that is a safer and less hazardous Coomassie alternative with comparable sensitivity
- One-Step Lumitein™: A red fluorescent stain at lower cost than SYPRO® Ruby, with comparable sensitivity
- One-Step Lumitein™ UV: A non-toxic and rapid red fluorescent protein gel stain optimized for UV transilluminators
We offer a broad selection of reagents and tools for western blotting including classic and novel blocking reagents, buffers, total protein prestains, and prestained protein ladders. View our Western Blotting technology page for more info.
- TrueBlack® Western Blot Blocking Buffer: A ready-to-use buffer system for fluorescence western blotting that provides optimal blocking of non-specific interactions associated with dye-labeled antibodies
- VersaBlot™ Total Protein Prestain: A highly linear and reversible protein prestain for downstream multi-color fluorescence western blotting
- Near-IR CF® Dye Conjugates: Our near-infrared CF® dyes offers greater linearity and multiplexing capabilities compared to chemiluminescence detection
The GloMelt™ Thermal Shift Protein Stability Kit is a low cost and rapid solution for analyzing protein thermal stability on PCR instruments.
- Universal: Green fluorescence optimal for qPCR instruments
- Versatile: Compatible with high detergent concentrations and tolerates a wide pH range, reducing agents, and common buffers/excipients
- Scalable for High Throughput Studies: Compatible with low reaction volumes and low protein concentrations
Our ViVoBrite™ Rapid Antibody Labeling Kits are ideal for preparing fluorescently labeled antibodies for in vivo imaging in small animals. Each kit contains one of our superior Near-IR CF® dyes which offer highly specific detection and strong tissue penetration for in vivo imaging. Learn more about Biotium’s superior Near-IR CF® dyes.
- Each kit contains sufficient reagents for 3 labeling reactions of 1 mg each
- Available with four industry-leading near-IR CF® dyes
- Includes buffers, ultrafiltration vials and syringe filters for labeling and purification
We offer a wide array of cellular stains for studying the disease state and cell response associated with COVID-19. Our novel stains for lysosomes, microtubules, and mitochondria are particularly useful for analyzing broad cellular changes during infection.
A key mechanism for infection by coronaviruses such as SARS-CoV-2 is the hijacking of endocytic processes that facilitate viral membrane fusion. Lysosomes play a critical role in these processes, particularly lysosome proteases which activate coronavirus spike proteins for viral entry into cells (Zheng et. al). LysoView™ dyes are lysosome specific, pH-sensitive fluorescent stains for live cells and are available in blue, green, visible red, and far-red fluorescence. These stains may be particularly useful for studying SARS-CoV-2 tropism with respect to lysosomes as well as analyzing cellular responses to potential therapeutics in vitro.
- Specific: Highly-selective lyosomotropic dyes that accumulate in acidic organelles
- Convenient: pH-dependent fluorescence in live cells without a wash step
- Choice: Available in 5 colors from blue to far-red fluorescence
Other Cellular Stains
See our full selection of novel and classic cellular stains for organelles and other cell structures.
- Mitochondria: MitoView™ dyes are membrane potential-dependent and -independent mitochondria stains available in 5 colors
- Nucleus: NucSpot™ and RedDot™ nuclear stains for live or fixed cells available in a variety of colors
- Cell Surface: Our line of CellBrite™ and MemBrite™ cell surface stains accommodate a broad range workflows for imaging in live or fixed cells.
- Microtubules: ViaFluor™ live-cell microtubule stains are cell-permeable microtubule probes for visualizing cytoskeleton. CF® Dye phalloidin conjugates are also available for staining actin filaments in fixed cells.