other fluorescent biotin

Biotin-4-fluorescein (N-(5-aminopentyl)biotinamide, trifluoroacetic acid salt) has been reported to be a superior fluorescent biotin derivative for accurate measurement of avidin and streptavidin in crude biological samples. Binding of biotin to avidin and streptavidin is high affinity, fast and noncooperative.

Biotin-AM3-25 is a conjugate of AM3-25 and biotin. Membranes labeled with the probe can subsequently couple to avidin or streptavidin.

We developed biotin-rhodamine 110 as an alternative to biotin-4-fluorescein (90062) and fluorescein biotin (80019), both of which have been used for detection of biotin binding sites and the degree of biotinylation of proteins, and for the measurement of avidin and streptavidin in crude biological samples.

Fluorescein biotin (5-((N-(5-(N-(6-(Biotinoyl)amino)hexanoyl)amino)pentyl)thioureidyl) fluorescein) can be used to detect and quantify biotin binding sites and the degree of biotinylation of proteins. Also see biotin-rhodamine 110 (80022) and biotin-4-fluorescein (90062).

This fluorescent biotin derivative is a useful tracer for studying neuronal morphology. The biotin portion of the molecule allows researchers to amplify the fluorescent signal using standard biotin-avidin or biotin-streptavidin biochemistry.

TMR biocytin is a cell-impermeant, fixable polar tracer that combines the tetramethylrhodamine (TAMRA) with biotin and an aldehyde-fixable primary amine.  Polar tracers are commonly used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis.