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Biotin-4-fluorescein

Biotin-4-fluorescein (N-(5-aminopentyl)biotinamide, trifluoroacetic acid salt) has been reported to be a superior fluorescent biotin derivative for accurate measurement of avidin and streptavidin in crude biological samples. Binding of biotin to avidin and streptavidin is high affinity, fast and noncooperative.

Product Attributes

Excitation/Emission

494/523 nm

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Product Description

Biotin-4-fluorescein (N-(5-aminopentyl)biotinamide, trifluoroacetic acid salt) has been reported to be a superior fluorescent biotin derivative for accurate measurement of avidin and streptavidin in crude biological samples. Binding of biotin to avidin and streptavidin is high affinity, fast and noncooperative.

  • λExEm = 494/523 nm
  • Yellow solid soluble in DMSO, or pH>7 buffers
  • Store at 4°C and protect from light
  • C33H32N4O8S
  • MW: 644.7

References

1. Biochim Biophys Acta 1427, 33 (1999).

2. Biochim Biophys Acta 1427, 44 (1999).

 

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