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Biotin-4-fluorescein

Biotin-4-fluorescein (N-(5-aminopentyl)biotinamide, trifluoroacetic acid salt) has been reported to be a superior fluorescent biotin derivative for accurate measurement of avidin and streptavidin in crude biological samples. Binding of biotin to avidin and streptavidin is high affinity, fast and noncooperative.

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Product Description

Biotin-4-fluorescein (N-(5-aminopentyl)biotinamide, trifluoroacetic acid salt) has been reported to be a superior fluorescent biotin derivative for accurate measurement of avidin and streptavidin in crude biological samples. Binding of biotin to avidin and streptavidin is high affinity, fast and noncooperative.

  • λExEm = 494/523 nm
  • Yellow solid soluble in DMSO, or pH>7 buffers
  • Store at 4°C and protect from light
  • C33H32N4O8S
  • MW: 644.7

References

1. Biochim Biophys Acta 1427, 33 (1999).

2. Biochim Biophys Acta 1427, 44 (1999).

 

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