ExoBrite™ EV Stain Enhancer (100X)
A unique additive that can be added to extracellular vesicle (EV) stain reactions to improve the staining specificity for applications like flow cytometry.
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Product Description
The ExoBrite™ EV Stain Enhancer is a unique additive that can be added to extracellular vesicle (EV) stain reactions to improve the staining specificity for applications like flow cytometry. The ExoBrite™ Stain Enhancer works by reducing the aggregation of certain EV stains, which allows the conjugate to stain the EVs more efficiently, resulting in a better signal-to-noise ratio and fewer false positives.
Features
- Improves signal-to-noise by reducing or eliminating aggregates of certain EV stains
- Validated with several different lectins and Annexin V
- Does not interfere with antibody staining of EVs
- Easy to use, just add directly to the staining reaction
Get precise, reliable results with a range of popular EV probes
Enhancer has been shown to be beneficial for staining EVs with WGA, other lectins, and Annexin V, but it is not recommended for lipophilic EV stains like ExoBrite™ True EV Membrane Stains or PKH dyes. Enhancer can be used to decrease aggregation of Cholera Toxin B (CTB), but generally is not required due to the intrinsically low aggregation of CTB conjugates. Enhancer is generally not required for use with ExoBrite™ antibody conjugates because they are already formulated to reduce aggregation, however, it may provide benefits for certain antibodies that do show aggregates in flow.
Learn about Biotium’s optimized ExoBrite™ stains and antibodies for EV research
Biotium’s ExoBrite™ True EV Membrane Stains are genuine lipophilic membrane dyes are designed for superior pan-EV labeling over other membrane dyes including PKH, DiO, DiI, and DiD. Biotium also offers
ExoBrite™ EV Surface Stains which are fluorescent conjugates of probes for labeling EV membrane surface targets. ExoBrite™ EV Surface Stains are available as cholera toxin subunit B (CTB), wheat germ agglutinin (WGA), or Annexin V conjugates. A convenient ExoBrite™ EV Surface Stain Sampler Kit is also available, the kit includes each ExoBrite™ EV Surface Stains (CTB, WGA, and Annexin V) for assessing which stain offers the best coverage for the EV samples of interest.
ExoBrite™ Antibody Conjugates are optimized for detection of CD9, CD63, and CD81 EV markers by flow cytometry and western blotting. For super-resolution imaging by STORM, learn about our ExoBrite™ STORM CTB EV Staining Kits available in four CF® Dyes validated for STORM.
ExoBrite™ EV Stains Comparison Guide
| ExoBrite™ EV Surface Stain | Pros | Cons |
|---|---|---|
| ExoBrite™ True EV Membrane Stains | • Near-complete staining of EVs in a sample • Broad compatibility with different EV sources • Validated for flow and fNTA | • Can't be used to stain bead-bound EVs • May have more aggregation than CTB & Annexin |
| ExoBrite™ Annexin EV Staining Kits | • Broad compatibility with different EV sources • Validated for flow and fNTA • Low background aggregates | • May not stain every EV in a sample • Doesn't work well on bead-bound EVs |
| ExoBrite™ WGA EV Staining Kits | • Broad compatibility with different EV sources • Can be used with bead-bound EVs | • May not stain every EV in a sample • Doesn't work well for fNTA |
| ExoBrite™ CTB EV Staining Kits | • Validated for flow and fNTA • Extremely low background | • May not stain every EV in a sample • Does not stain EVs from every source |
| ExoBrite™ Antibodies | • Highly specific for human tetraspanins CD9, CD63, or CD81 • Validated for EV flow • Broad compatibility for different EV sources • Can be used with bead-bound EVs • Can be used for WB | • Depends on the expression level of the target protein on the EVs |