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ExoBrite™ EV Surface Stain Sampler Kit, Green

Kit includes each of ExoBrite™ 490/515 EV Surface Stains (CTB, WGA, and Annexin V) for assessing which stain offers the best coverage for EV samples of interest.

Product Attributes


ExoBrite™ 490/515



Catalog #
1 kit
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Product Description

The ExoBrite™ EV Surface Stain Sampler Kit, Green was developed to offer each of Biotium’s ExoBrite™ EV Surface Stains (CTB, WGA, and Annexin V) for assessing which stain offers the best coverage for the EV samples of interest.


  • Optimally formulated ExoBrite™ 490/515 Annexin, CTB, and WGA conjugates for staining purified EVs
  • Broad compatibility with EVs isolated from different sources
  • Designed for detection by flow cytometry
  • Bright signal and low background
  • Compatible with antibody co-staining

Kit Components

  • ExoBrite™ 490/515 Annexin EV Stain
  • ExoBrite™ 490/515 WGA EV Stain
  • ExoBrite™ 490/515 CTB EV Stain
  • ExoBrite™ Reconstitution Solution
  • ExoBrite™ 1X PBS Solution
  • 50X Annexin Binding Buffer

Bright & Specific EV Staining with Minimal Aggregation

ExoBrite™ EV Surface Stains are conjugates of probes for labeling EV membrane surface targets using Biotium’s unique fluorescent dyes for superior brightness and specificity. The stains were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. For example, some lipophilic membrane dyes used to stain EVs can form aggregates of a similar size as exosomes or EVs, thus confounding analysis. ExoBrite™ EV stains have been formulated for bright and specific staining of EV surface targets with minimal aggregation in flow cytometry. In addition, ExoBrite™ EV Stains do not bind non-specifically to polystyrene beads, and therefore unlike hydrophobic membrane dyes, they can be used to stain bead-bound EVs.

A Convenient Sampler Kit for Comparing Samples

A major issue for EV detection includes varying signal and coverage using tetraspanin antibody staining of EVs isolated from different cell types or biological fluids. The ExoBrite™ EV Surface Stain Sampler Kit, Green includes each of Biotium’s ExoBrite™ EV Surface Stains (CTB, WGA, and Annexin V) to allow users to assess which stain(s) offer the best coverage for their EV samples. ExoBrite™ CTB EV Stains are conjugates of cholera toxin subunit B (CTB), which binds to GM1 gangliosides that are found on the surface of mammalian lipid rafts and some EV populations. ExoBrite™ WGA EV Stains are uniquely formulated conjugates of wheat germ agglutinin (WGA), a carbohydrate-binding lectin with high affinity for N-acetylglucosamine moieties of glycoproteins frequently exposed on EV membranes. ExoBrite™ Annexin V EV Stains is a calcium-dependent phospholipid-binding protein with high affinity for phosphatidyleserine (PS), which is exposed on the surface of apoptotic cells and also used as a marker for EV from a variety of sources.

EVs are often labeled with fluorescent antibodies targeting one or more of the tetraspanin proteins CD9, CD63, and CD81. ExoBrite™ EV Stains can be combined with antibody staining, for multi-parameter analysis. Biotium offers a selection of fluorescent ExoBrite™ Flow Antibodies against CD9, CD63, and CD81 that are optimized for detection of free or bead-bound EVs by flow cytometry.

Note: ExoBrite™ EV Stains have been found to label EVs derived from several tested cell lines (see Validated EV Sources below), but may not stain EVs from every source.

Validated EV Sources for ExoBrite™ EV Surface Stains

EV SourceExoBrite™ True EV Membrane StainsExoBrite™ CTB StainsExoBrite™ WGA StainsExoBrite™ Annexin Stains
A549 cellsYesYesYesYes
CHO cellsYesNoYesYes
hASC (human adipose stem cells)NDNo1NDND
HeLa cellsYesNoYesYes
HUVEC (human umbilical vein endothelial cells)NDNo1NDND
J774 cellsYesYesYesYes
Jurkat cellsYesYesYesYes
MCF-7 cellsYesYesYesYes
Raji cellsNDYesYesYes
Skeletal myoblastsNDYes1NDND
U2OS cellsYesNoYesYes
U937 cellsYesNoYesYes
1Customer-reported data
Value of “Yes” or “No” indicates coverage of EVs based on Biotium’s internal data or customer-reported data. Value of “ND” indicates no data.

Biotium also offers other validated ExoBrite™ reagents for flow cytometry, western blotting, or super-resolution imaging.

Learn about Biotium’s new ExoBrite™ True EV Membrane Stains. These genuine lipophilic membrane dyes are designed for superior pan-EV labeling over other membrane dyes including PKH, DiO, DiI, and DiD. Biotium also offers ExoBrite™ Antibody Conjugates for optimal detection of CD9, CD63, and CD81 EV markers by flow cytometry and western blotting. For super-resolution imaging by STORM, learn about our ExoBrite™ STORM CTB EV Staining Kits available in four CF® Dyes validated for STORM.


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