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ExoBrite™ WGA EV Staining Kits

Fluorescent WGA conjugates that are optimized for bright staining of extracellular vesicles for flow cytometry.

Product Attributes


ExoBrite™ 410/450, ExoBrite™ 490/515, ExoBrite™ 560/585, ExoBrite™ 640/660


Blue, Green, Orange-red, Far-red

Catalog #
100 labelings
500 labelings
Clear selection
(Please select quantity above )

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Product Description

ExoBrite™ WGA EV Staining Kits were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. ExoBrite™ WGA EV Stains bind to molecules in the EV membrane for bright, specific staining, with little background.


  • Optimally formulated WGA conjugates for staining purified EVs
  • Broad compatibility, stained EVs isolated from all 9 sources tested
  • Designed for detection by flow cytometry
  • Bright signal and low background
  • Compatible with antibody co-staining
  • Available in 4 colors

Kit Components

  • ExoBrite™ WGA EV Stain
  • ExoBrite™ 1X PBS Solution

ExoBrite™ WGA EV Stains are uniquely formulated conjugates of wheat germ agglutinin (WGA), a carbohydrate-binding lectin with high affinity for N-acetylglucosamine moieties of glycoproteins. WGA conjugates are often used for labeling cell membranes as well as gram-positive bacteria. WGA conjugates have also been used to detect EVs due to the presence of glycoproteins on EV membranes.

ExoBrite™ WGA EV Stains were designed to overcome some of the challenges of detecting isolated EVs, particularly in flow cytometry. For example, tetraspanin antibodies commonly used to stain EVs can have varying signal and coverage depending on the EV source. Conversely, ExoBrite™ WGA EV Stains show bright staining of EVs derived from a broad range of sources. We tested EVs derived from 9 cell lines and ExoBrite™ WGA EV Stains showed strong staining for all of them. ExoBrite™ WGA EV Stains are less prone to aggregation than hydrophobic membrane dyes and do not bind non-specifically to polystyrene beads, allowing them to be used to stain bead-bound EVs.

EVs are often labeled with fluorescent antibodies targeting one or more of the tetraspanin proteins CD9, CD63, and CD81. ExoBrite™ WGA staining can be combined with antibody staining, for multi-parameter analysis.

Note: ExoBrite™ WGA EV Stains have been found to label EVs derived from all cell lines tested (see Validated EV Sources below), but may not stain EVs from every source.

ExoBrite™ WGA EV Staining Kits

ProductEx/EmDetection channelsSizeCatalog Number
ExoBrite™ 410/450
WGA EV Staining Kit
416/452 nmPacific Blue™100 Labelings30123-T
500 Labelings30123
ExoBrite™ 490/515
WGA EV Staining Kit
490/516 nmFITC100 Labelings30124-T
500 Labelings30124
ExoBrite™ 560/585
WGA EV Staining Kit
562/584 nmPE100 Labelings30125-T
500 Labelings30125
ExoBrite™ 640/660
WGA EV Staining Kit
642/663 nmAPC100 Labelings30126-T
500 Labelings30126

Validated EV Sources for ExoBrite™ EV Surface Stains

EV SourceExoBrite™ True EV Membrane StainsExoBrite™ CTB StainsExoBrite™ WGA StainsExoBrite™ Annexin Stains
A549 cellsYesYesYesYes
CHO cellsYesNoYesYes
hASC (human adipose stem cells)NDNo1NDND
HeLa cellsYesNoYesYes
HUVEC (human umbilical vein endothelial cells)NDNo1NDND
J774 cellsYesYesYesYes
Jurkat cellsYesYesYesYes
MCF-7 cellsYesYesYesYes
Raji cellsNDYesYesYes
Skeletal myoblastsNDYes1NDND
U2OS cellsYesNoYesYes
U937 cellsYesNoYesYes
1Customer-reported data
Value of “Yes” or “No” indicates coverage of EVs based on Biotium’s internal data or customer-reported data. Value of “ND” indicates no data.

Biotium also offers other validated ExoBrite™ reagents for flow cytometry, western blotting, or super-resolution imaging.

Learn about Biotium’s new ExoBrite™ True EV Membrane Stains. These genuine lipophilic membrane dyes are designed for superior pan-EV labeling over other membrane dyes including PKH, DiO, DiI, and DiD. Biotium also offers ExoBrite™ CTB EV Stains (cholera toxin B conjugates) and ExoBrite™ Annexin EV Stains optimized for bright and sensitive staining of EVs. The ExoBrite™ EV Surface Stain Sampler Kit contains each of Biotium’s ExoBrite™ EV Surface Stains (CTB, WGA, and Annexin V) for assessing which stain offers the best coverage for the EV samples of interest. Biotium also offers ExoBrite™ Antibody Conjugates for optimal detection of CD9, CD63, and CD81 EV markers by flow cytometry and western blotting. For super-resolution imaging by STORM, learn about our ExoBrite™ STORM CTB EV Staining Kits available in four CF® Dyes validated for STORM.


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