ExoBrite™ WGA EV Staining Kits
Fluorescent WGA conjugates that are optimized for bright staining of extracellular vesicles for flow cytometry.
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Product Description
ExoBrite™ WGA EV Staining Kits were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. ExoBrite™ WGA EV Stains bind to molecules in the EV membrane for bright, specific staining, with little background.
Features
- Optimally formulated WGA conjugates for staining purified EVs
- Broad compatibility, stained EVs isolated from all 9 sources tested
- Designed for detection by flow cytometry
- Bright signal and low background
- Compatible with antibody co-staining
- Available in 4 colors
Kit Components
- ExoBrite™ WGA EV Stain
- ExoBrite™ 1X PBS Solution
ExoBrite™ WGA EV Stains are uniquely formulated conjugates of wheat germ agglutinin (WGA), a carbohydrate-binding lectin with high affinity for N-acetylglucosamine moieties of glycoproteins. WGA conjugates are often used for labeling cell membranes as well as gram-positive bacteria. WGA conjugates have also been used to detect EVs due to the presence of glycoproteins on EV membranes.
ExoBrite™ WGA EV Stains were designed to overcome some of the challenges of detecting isolated EVs, particularly in flow cytometry. For example, tetraspanin antibodies commonly used to stain EVs can have varying signal and coverage depending on the EV source. Conversely, ExoBrite™ WGA EV Stains show bright staining of EVs derived from a broad range of sources. We tested EVs derived from 9 cell lines and ExoBrite™ WGA EV Stains showed strong staining for all of them. ExoBrite™ WGA EV Stains are less prone to aggregation than hydrophobic membrane dyes and do not bind non-specifically to polystyrene beads, allowing them to be used to stain bead-bound EVs.
EVs are often labeled with fluorescent antibodies targeting one or more of the tetraspanin proteins CD9, CD63, and CD81. ExoBrite™ WGA staining can be combined with antibody staining, for multi-parameter analysis.
Note: ExoBrite™ WGA EV Stains have been found to label EVs derived from all cell lines tested (see Validated EV Sources below), but may not stain EVs from every source.
ExoBrite™ WGA EV Staining Kits
Product | Ex/Em | Detection channels | Size | Catalog Number |
---|---|---|---|---|
ExoBrite™ 410/450 WGA EV Staining Kit | 416/452 nm | Pacific Blue™ | 100 Labelings | 30123-T |
500 Labelings | 30123 | |||
ExoBrite™ 490/515 WGA EV Staining Kit | 490/516 nm | FITC | 100 Labelings | 30124-T |
500 Labelings | 30124 | |||
ExoBrite™ 560/585 WGA EV Staining Kit | 562/584 nm | PE | 100 Labelings | 30125-T |
500 Labelings | 30125 | |||
ExoBrite™ 640/660 WGA EV Staining Kit | 642/663 nm | APC | 100 Labelings | 30126-T |
500 Labelings | 30126 |
Validated EV Sources for ExoBrite™ EV Surface Stains
EV Source | ExoBrite™ CTB Stains | ExoBrite™ WGA Stains | ExoBrite™ Annexin Stains |
---|---|---|---|
A549 cells | Yes | Yes | Yes |
CHO cells | No | Yes | Yes |
hASC (human adipose stem cells) | No1 | ND | ND |
HeLa cells | No | Yes | Yes |
HUVEC (human umbilical vein endothelial cells) | No1 | ND | ND |
J774 cells | Yes | Yes | Yes |
Jurkat cells | Yes | Yes | Yes |
MCF-7 cells | Yes | Yes | Yes |
Plasma | No | ND | Yes |
Raji cells | Yes | Yes | Yes |
Serum | No | ND | Yes |
Skeletal myoblasts | Yes1 | ND | ND |
U2OS cells | No | Yes | Yes |
U937 cells | No | Yes | Yes |
Value of “Yes” or “No” indicates coverage of EVs based on Biotium’s internal data or customer-reported data. Value of “ND” indicates no data.
Biotium also offers other validated ExoBrite™ reagents for flow cytometry, western blotting, or super-resolution imaging.
Learn about ExoBrite™ Antibody Conjugates for optimal detection of CD9, CD63, and CD81 EV markers by flow cytometry and western blotting. Biotium also offers ExoBrite™ CTB EV Stains (cholera toxin B conjugates) and ExoBrite™ Annexin EV Stains optimized for bright and sensitive staining of EVs and exosomes. For super-resolution imaging by STORM, learn about our ExoBrite™ STORM CTB EV Staining Kits available in four CF® Dyes validated for STORM.