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ExoBrite™ Annexin EV Staining Kits

Fluorescent Annexin V conjugates that are optimized for bright and low background staining of extracellular vesicles for flow cytometry.

Product Attributes


ExoBrite™ 410/450, ExoBrite™ 490/515, ExoBrite™ 560/585, ExoBrite™ 655/670


Blue, Green, Orange-red, Far-red

Catalog #
100 labelings
500 labelings
Clear selection
(Please select quantity above )

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Product Description

ExoBrite™ Annexin EV Staining Kits were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. ExoBrite™ Annexin EV Stains bind to molecules in the EV membrane for bright, specific staining, with little to no background.


  • Optimally formulated Annexin V conjugates for staining purified EVs
  • Broad compatibility, stained EVs isolated from all 9 sources tested
  • Designed for detection by flow cytometry
  • Bright signal and low background
  • Compatible with antibody co-staining
  • Available in 4 colors

Kit Components

  • ExoBrite™ Annexin EV Stain
  • 50X Annexin Binding Buffer

Validated EV Sources

  • MCF-7, J774, U2OS, Jurkat,
    HeLa, Raji, CHO, U937, A549

ExoBrite™ Annexin EV Stains are uniquely formulated conjugates of Annexin V, a 35-36 kDA calcium-dependent phospholipid-binding protein with high affinity for phosphatidyleserine (PS). Annexin V conjugates have been used to detect EVs due to the presence of PS on most EV membranes.

ExoBrite™ Annexin EV Stains were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. For example, lipophilic membrane dyes commonly used to stain EVs can form aggregates of a similar size as exosomes or EVs, thus confounding analysis. Conversely, ExoBrite™ Annexin EV Stains are specially formulated to minimize aggregation in flow cytometry, allowing EVs to be identified with bright staining with minimal background. In addition, ExoBrite™ Annexin EV Stains were designed to offer broad coverage of EVs isolated from different sources. We tested EVs derived from 9 cell lines and ExoBrite™ Annexin EV Stains showed strong staining for all of them.

EVs are often labeled with fluorescent antibodies targeting one or more of the tetraspanin proteins CD9, CD63, and CD81. ExoBrite™ Annexin staining can be combined with antibody staining, for multi-parameter analysis.

Note: ExoBrite™ Annexin EV Stains have been found to label EVs derived from all cell lines tested (see Validated EV Sources below), but may not stain EVs from every source.

ExoBrite™ Annexin EV Staining Kits

ProductEx/EmDetection channelsSizeCatalog Number
ExoBrite™ 410/450
Annexin EV Staining Kit
416/452 nmPacific Blue™100 Labelings30119-T
500 Labelings30119
ExoBrite™ 490/515
Annexin EV Staining Kit
490/516 nmFITC100 Labelings30120-T
500 Labelings30120
ExoBrite™ 560/585
Annexin EV Staining Kit
562/584 nmPE100 Labelings30121-T
500 Labelings30121
ExoBrite™ 655/670
Annexin EV Staining Kit
652/668 nmAPC100 Labelings30122-T
500 Labelings30122

Validated EV Sources for ExoBrite™ EV Surface Stains

EV SourceExoBrite™ True EV Membrane StainsExoBrite™ CTB StainsExoBrite™ WGA StainsExoBrite™ Annexin Stains
A549 cellsYesYesYesYes
CHO cellsYesNoYesYes
hASC (human adipose stem cells)NDNo1NDND
HeLa cellsYesNoYesYes
HUVEC (human umbilical vein endothelial cells)NDNo1NDND
J774 cellsYesYesYesYes
Jurkat cellsYesYesYesYes
MCF-7 cellsYesYesYesYes
Raji cellsNDYesYesYes
Skeletal myoblastsNDYes1NDND
U2OS cellsYesNoYesYes
U937 cellsYesNoYesYes
1Customer-reported data
Value of “Yes” or “No” indicates coverage of EVs based on Biotium’s internal data or customer-reported data. Value of “ND” indicates no data.

Biotium also offers other validated ExoBrite™ reagents for flow cytometry, western blotting, or super-resolution imaging.

Learn about Biotium’s new ExoBrite™ True EV Membrane Stains. These genuine lipophilic membrane dyes are designed for superior pan-EV labeling over other membrane dyes including PKH, DiO, DiI, and DiD. Biotium also offers ExoBrite™ CTB EV Stains (cholera toxin B conjugates) and ExoBrite™ WGA EV Stains (wheat germ agglutinin) optimized for bright and sensitive staining of EVs. The ExoBrite™ EV Surface Stain Sampler Kit contains each of Biotium’s ExoBrite™ EV Surface Stains (CTB, WGA, and Annexin V) for assessing which stain offers the best coverage for the EV samples of interest. Biotium also offers ExoBrite™ Antibody Conjugates for optimal detection of CD9, CD63, and CD81 EV markers by flow cytometry and western blotting. For super-resolution imaging by STORM, learn about our ExoBrite™ STORM CTB EV Staining Kits available in four CF® Dyes validated for STORM.

Note: We do not recommend using ExoBrite™ 410/450 Annexin EV Stain or ExoBrite™ 490/515 Annexin EV Stain to stain bead-bound EVs. For bead-bound EVs we recommend using ExoBrite™ 560/585 Annexin EV Stain, ExoBrite™ 655/670 Annexin EV Stain, as well as ExoBrite™ CTB EV Stains.


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