ExoBrite™ Annexin EV Staining Kits
Fluorescent Annexin V conjugates that are optimized for bright and low background staining of extracellular vesicles for flow cytometry.
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Product Description
ExoBrite™ Annexin EV Staining Kits were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. ExoBrite™ Annexin EV Stains bind to molecules in the EV membrane for bright, specific staining, with little to no background.
Features
- Optimally formulated Annexin V conjugates for staining purified EVs
- Broad compatibility, stained EVs isolated from all 9 sources tested
- Designed for detection by flow cytometry
- Bright signal and low background
- Compatible with antibody co-staining
- Available in 4 colors
Kit Components
- ExoBrite™ Annexin EV Stain
- 50X Annexin Binding Buffer
Validated EV Sources
- MCF-7, J774, U2OS, Jurkat,
HeLa, Raji, CHO, U937, A549
ExoBrite™ Annexin EV Stains are uniquely formulated conjugates of Annexin V, a 35-36 kDA calcium-dependent phospholipid-binding protein with high affinity for phosphatidyleserine (PS). Annexin V conjugates have been used to detect EVs due to the presence of PS on most EV membranes.
ExoBrite™ Annexin EV Stains were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. For example, lipophilic membrane dyes commonly used to stain EVs can form aggregates of a similar size as exosomes or EVs, thus confounding analysis. Conversely, ExoBrite™ Annexin EV Stains are specially formulated to minimize aggregation in flow cytometry, allowing EVs to be identified with bright staining with minimal background. In addition, ExoBrite™ Annexin EV Stains were designed to offer broad coverage of EVs isolated from different sources. We tested EVs derived from 9 cell lines and ExoBrite™ Annexin EV Stains showed strong staining for all of them.
EVs are often labeled with fluorescent antibodies targeting one or more of the tetraspanin proteins CD9, CD63, and CD81. ExoBrite™ Annexin staining can be combined with antibody staining, for multi-parameter analysis.
Note: ExoBrite™ Annexin EV Stains have been found to label EVs derived from all cell lines tested (see Validated EV Sources below), but may not stain EVs from every source.
ExoBrite™ Annexin EV Staining Kits
Product | Ex/Em | Detection channels | Size | Catalog Number |
---|---|---|---|---|
ExoBrite™ 410/450 Annexin EV Staining Kit | 416/452 nm | Pacific Blue™ | 100 Labelings | 30119-T |
500 Labelings | 30119 | |||
ExoBrite™ 490/515 Annexin EV Staining Kit | 490/516 nm | FITC | 100 Labelings | 30120-T |
500 Labelings | 30120 | |||
ExoBrite™ 560/585 Annexin EV Staining Kit | 562/584 nm | PE | 100 Labelings | 30121-T |
500 Labelings | 30121 | |||
ExoBrite™ 655/670 Annexin EV Staining Kit | 652/668 nm | APC | 100 Labelings | 30122-T |
500 Labelings | 30122 |
Validated EV Sources for ExoBrite™ EV Surface Stains
EV Source | ExoBrite™ CTB Stains | ExoBrite™ WGA Stains | ExoBrite™ Annexin Stains |
---|---|---|---|
A549 cells | Yes | Yes | Yes |
CHO cells | No | Yes | Yes |
hASC (human adipose stem cells) | No1 | ND | ND |
HeLa cells | No | Yes | Yes |
HUVEC (human umbilical vein endothelial cells) | No1 | ND | ND |
J774 cells | Yes | Yes | Yes |
Jurkat cells | Yes | Yes | Yes |
MCF-7 cells | Yes | Yes | Yes |
Plasma | No | ND | Yes |
Raji cells | Yes | Yes | Yes |
Serum | No | ND | Yes |
Skeletal myoblasts | Yes1 | ND | ND |
U2OS cells | No | Yes | Yes |
U937 cells | No | Yes | Yes |
Value of “Yes” or “No” indicates coverage of EVs based on Biotium’s internal data or customer-reported data. Value of “ND” indicates no data.
Biotium also offers other validated ExoBrite™ reagents for flow cytometry, western blotting, or super-resolution imaging.
Learn about ExoBrite™ Antibody Conjugates for optimal detection of CD9, CD63, and CD81 EV markers by flow cytometry and western blotting. Biotium also offers ExoBrite™ CTB EV Stains (cholera toxin B conjugates) optimized for bright and sensitive staining of EVs and exosomes. For super-resolution imaging by STORM, learn about our ExoBrite™ STORM CTB EV Staining Kits available in four CF® Dyes validated for STORM.
Note: We do not recommend using ExoBrite™ 410/450 Annexin EV Stain or ExoBrite™ 490/515 Annexin EV Stain to stain bead-bound EVs. For bead-bound EVs we recommend using ExoBrite™ 560/585 Annexin EV Stain, ExoBrite™ 655/670 Annexin EV Stain, as well as ExoBrite™ CTB EV Stains.