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ExoBrite™ CTB EV Staining Kits

Fluorescent cholera toxin subunit B (CTB) conjugates that are optimized for bright and clean staining of extracellular vesicles for flow cytometry.

Product Attributes

Dye

ExoBrite™ 410/450, ExoBrite™ 490/515, ExoBrite™ 560/585, ExoBrite™ 640/660

Colors

Blue, Green, Orange-red, Far-red

Dye
Size
Catalog #
price
Qty
100 labelings
500 labelings
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Product Description

ExoBrite™ CTB EV Staining Kits were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. ExoBrite™ CTB EV Stains bind to molecules in the EV membrane for bright, specific staining, with little to no background.

Features

  • Optimally formulated CTB conjugates for staining EVs
  • Designed for detection by flow cytometry
  • Bright signal and low background
  • Stain purified or bead-bound EVs
  • Compatible with antibody co-staining
  • Available in 4 colors

Kit Components

  • ExoBrite™ CTB EV Stain
  • ExoBrite™ Reconstitution Solution
  • 1 vial of stain makes 100 uL of 500X staining solution

Note: The name of this product has been revised from ExoBrite™ EV Membrane Staining Kits.

ExoBrite™ CTB EV Stains are optimally formulated fluorescent conjugates of cholera toxin subunit B (CTB), which binds to GM1 gangliosides that are commonly found on the surface of mammalian lipid rafts and EVs. The stains were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. Some dyes used to stain EVs can form aggregates of a similar size as exosomes or EVs, thus confounding analysis. ExoBrite™ CTB EV Stains, however, were formulated to show little to no aggregation in flow cytometry, allowing EVs to be identified with bright and specific staining. Unlike hydrophobic membrane dyes, ExoBrite™ CTB EV Stains do not bind non-specifically to polystyrene beads, meaning that they can be used to stain bead-bound EVs.

EVs are often labeled with fluorescent antibodies targeting one or more of the tetraspanin proteins CD9, CD63, and CD81. ExoBrite™ CTB staining can be combined with antibody staining, for multi-parameter analysis.

Note: Staining signal and coverage can vary significantly between EVs from different sources. Please see the table below for a list of EV sources that ExoBrite™ CTB EV Stains  have shown either robust or poor staining based on internal data or customer data.

Less background and better coverage over other EV stains

ExoBrite™ CTB EV Stains were designed to offer exceptional signal:noise and more complete coverage of purified and bead-bound EVs. In the figure below, lipophilic dye DiO and plasma membrane stain CellMask™ demonstrate an unacceptable amount of dye aggregation in gated EVs. Other EV stains such as ExoFlow-ONE™ and ExoGlow™ have less coverage of EVs when compared to ExoBrite™ CTB EV Stains.

ExoBrite™ CTB EV Stains have less background and more complete staining of extracellular vesicles (EVs) than other classic and competitor dyes. EVs were purified from MCF-7 cell supernatant using size exclusion chromatography (SEC). The purified EVs were stained in PBS with the indicated dyes (top row). The stained EV population was gated, with the number showing the percentage of particles falling within the exosome gate. Each dye was also added to filtered PBS (bottom row), to look for dye aggregation and non-specific background. Red arrows indicate these dye aggregates. Lipophilic dyes like DiO show a high number of particles of similar size to EVs, making them unsuited for small particle staining. CellMask™ also shows an unacceptable amount of dye aggregation falling within the EV gate. The ExoFlow-ONE™ and ExoGlow™ dyes form aggregates that can mostly be gated away from the EVs, but they also show less-complete coverage of EVs than ExoBrite™ CTB stains. (Click to enlarge).

ExoBrite™ CTB EV Staining Kits

ProductEx/EmDetection channelsSizeCatalog Number
ExoBrite™ 410/450
CTB EV Staining Kit
416/452 nmPacific Blue™100 Labelings30111-T
500 Labelings30111
ExoBrite™ 490/515
CTB EV Staining Kit
490/516 nmFITC100 Labelings30112-T
500 Labelings30112
ExoBrite™ 560/585
CTB EV Staining Kit
562/584 nmPE, Cy®3100 Labelings30113-T
500 Labelings30113
ExoBrite™ 640/660
CTB EV Staining Kit
642/663 nmAPC100 Labelings30114-T
500 Labelings30114

Validated EV Sources for ExoBrite™ CTB EV Stains

EV SourceBiotium DataCustomer Reported
A549 cellsHigh
CHO cellsLow
hASC (human adipose stem cells)Low
HeLa cellsLow
HUVEC (human umbilical vein endothelial cells)Low
J774 cellsHigh
Jurkat cellsHigh
MCF-7 cellsHigh
PlasmaLow
Raji cellsHigh
Skeletal myoblastsHigh
U2OS cellsLow
U937 cellsLow

Biotium also offers other validated ExoBrite™ reagents for flow cytometry, western blotting, or super-resolution imaging.

Learn about ExoBrite™ Antibody Conjugates for optimal detection of CD9, CD63, and CD81 EV markers by flow cytometry and western blotting. Biotium also offers ExoBrite™ WGA EV Stains (wheat germ agglutinin) and ExoBrite™ Annexin EV Stains optimized for bright and sensitive staining of EVs and exosomes. For super-resolution imaging by STORM, learn about our ExoBrite™ STORM CTB EV Staining Kits available in four CF® Dyes validated for STORM.

CellMask is a trademark of Thermo Fisher Scientific; ExoFlow-ONE and ExoGlow are trademarks of System Biosciences.
 

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