ExoBrite™ EV Membrane Staining Kits were designed to overcome some of the challenges of exosome detection, particularly in flow cytometry. ExoBrite™ stains bind to molecules in the exosome membrane for bright, specific staining, with little to no background.
- Stain the membranes of EVs and exosomes
- Stain both purified and bead-bound exosomes
- Bright signal and low background
- Designed for detection by flow cytometry
- Compatible with antibody co-staining
- Available in 2 colors (more coming soon)
- 1 vial makes 100 uL of 500X staining solution
- ExoBrite™ EV Membrane Stain
- ExoBrite™ Reconstitution Solution
Extracellular vesicles (EVs), including exosomes, are released from cells and are able to carry biological information between cells in different parts of the body. Therefore exosomes are increasingly studied for their potential use in drug delivery and medical diagnostic applications. Biotium developed ExoBrite™ EV Membrane Staining Kits for fluorescent labeling and detection of EVs and exosomes by flow cytometry. Other potential applications include fluorescence microscopy or other fluorescent exosome detection systems.
ExoBrite™ stains were designed to overcome some of the challenges of exosome detection, particularly in flow cytometry. While some dyes may form aggregates of a similar size as exosomes or EVs, thus confounding analysis, ExoBrite™ stains show little to no background, allowing stained exosomes to be accurately identified. ExoBrite™ stains bind to molecules in the exosome membrane for bright, specific staining. Unlike most other membrane dyes, ExoBrite™ stains do not bind non-specifically to polystyrene beads, meaning that they can be used to stain bead-bound exosomes.
Exosomes are often detected by using fluorescent antibodies targeting one or more of the tetraspanin proteins CD9, CD63, & CD81. ExoBrite™ EV Membrane Stains can be combined with antibody staining, for multi-parameter analysis. Biotium sells CD9 and CD81 monoclonal antibodies verified for exosome staining, available purified or conjugated to a large selection of bright and soluble CF® Dyes.