Support & Resources

+1

ExoBrite™ EV Membrane Staining Kits

Fluorescent staining kits for bright, clean staining of EVs and exosomes. Suitable for detection by flow cytometry or microscopy.

Product Attributes

Dye

ExoBrite™ 410/450, ExoBrite™ 490/515, ExoBrite™ 560/585, ExoBrite™ 640/660

Colors

Blue, Green, Orange-red, Far-red

Dye
Size
Catalog #
price
Qty
100 labelings
500 labelings
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Product Description

ExoBrite™ EV Membrane Staining Kits were designed to overcome some of the challenges of exosome detection, particularly in flow cytometry. ExoBrite™ stains bind to molecules in the exosome membrane for bright, specific staining, with little to no background.

Features

  • EV and exosome membrane stains
  • Designed for detection by flow cytometry
  • Bright signal and low background
  • Stain purified or bead-bound exosomes
  • Compatible with antibody co-staining
  • Available in 4 colors

Kit Components

  • ExoBrite™ EV Membrane Stain
  • ExoBrite™ Reconstitution Solution
  • 1 vial of stain makes 100 uL of 500X staining solution

Extracellular vesicles (EVs), including exosomes, are released from cells and are able to carry biological information between cells in different parts of the body. Therefore exosomes are increasingly studied for their potential use in drug delivery and medical diagnostic applications. Biotium developed ExoBrite™ EV Membrane Staining Kits for fluorescent labeling and detection of EVs and exosomes by flow cytometry. Other potential applications include fluorescence microscopy or other fluorescent exosome detection systems.

ExoBrite™ stains were designed to overcome some of the challenges of exosome detection, particularly in flow cytometry. While some dyes may form aggregates of a similar size as exosomes or EVs, thus confounding analysis, ExoBrite™ stains show little to no background, allowing stained exosomes to be accurately identified. ExoBrite™ stains bind to molecules in the exosome membrane for bright, specific staining. Unlike most other membrane dyes, ExoBrite™ stains do not bind non-specifically to polystyrene beads, meaning that they can be used to stain bead-bound exosomes.

Exosomes are often detected by using fluorescent antibodies targeting one or more of the tetraspanin proteins CD9, CD63, & CD81. ExoBrite™ EV Membrane Stains can be combined with antibody staining, for multi-parameter analysis. Biotium sells CD9 and CD81 monoclonal antibodies verified for exosome staining, available purified or conjugated to a large selection of bright and soluble CF® Dyes.

Less background and better coverage over other exosome/EV stains

ExoBrite™ EV Membrane Stains were designed to offer exceptional signal:noise and more complete coverage of purified and bead-bound exosomes. In the figure below, lipophilic dye DiO and plasma membrane stain CellMask™ demonstrate an unacceptable amount of dye aggregation in gated exosomes. Other exosome stains such as ExoFlow-ONE™ and ExoGlow™ have less dye aggregation but also less coverage of exosomes when compared to ExoBrite™ membrane stains.

ExoBrite™ EV Membrane Stains have less background and more complete exosome staining than other classic dye or competitor dyes. Exosomes were purified from MCF-7 cell supernatant using size exclusion chromatography (SEC). The purified exosomes were stained in PBS with the indicated dyes (top row). The exosome population was gated, with the number showing the percentage of particles falling within the exosome gate. Each dye was also added to filtered PBS (bottom row), to look for dye aggregation and non-specific background. Red arrows indicate these dye aggregates (Click to enlarge).
SEC-purified MCF-7-derived exosomes were stained with ExoBrite™ EV Membrane Stains (Click to enlarge).
ProductEx/EmDetection channelsSizeCatalog Number
ExoBrite™ 410/450
EV Membrane Staining Kit
416/452 nmPacific Blue™100 Labelings30111-T
500 Labelings30111
ExoBrite™ 490/515
EV Membrane Staining Kit
490/516 nmFITC100 Labelings30112-T
500 Labelings30112
ExoBrite™ 560/585
EV Membrane Staining Kit
562/584 nmPE, Cy®3100 Labelings30113-T
500 Labelings30113
ExoBrite™ 640/660
EV Membrane Staining Kit
642/663 nmAPC100 Labelings30114-T
500 Labelings30114

Biotium also offers ExoBrite™ Antibody Conjugates validated for flow cytometry or western blotting

Learn more about ExoBrite™ Antibody Conjugates for optimal detection of CD9, CD63, and CD81 exosome markers by flow cytometry and western blotting.

CellMask is a trademark of Thermo Fisher Scientific; ExoFlow-ONE and ExoGlow are trademarks of System Biosciences.
 

You may also like…