ExoBrite™ Calnexin Western Antibody is validated by Biotium for detection of calnexin, an endoplasmic reticulum protein (ER) that is not found in exosomes. The antibody serves as a negative control to assess the purity of isolated exosome extracts. The antibody is offered as a conjugate to ExoBrite™ 770/800 near-infrared fluorescent dye, which offers greater signal-to-noise than visible light fluorescent dyes for western blotting.
- Validated for detection of ER protein calnexin by fluorescent western blot
- Serves as a negative control for assessing purity of isolated exosome extracts
- Bright signal and low background
- Available in near-infrared ExoBrite™ 770/800.
EVs, including exosomes, are lipid-bound vesicles that are released from cells. EVs display specific surface proteins and can carry nucleic acids and other cargo, allowing them to transfer biological information between cells in different parts of the body. Therefore exosomes are increasingly studied for their potential use in drug delivery and medical diagnostic applications. The most common proteins used as exosome markers are CD9, CD63, and CD81, members of the tetraspanin family. Tetraspanins are plasma membrane proteins with many proposed functions, including activation and sorting of other membrane proteins. They are also thought to play a role in the targeting of proteins to multivesicular bodies (MVBs) and exosomes. These tetraspanins are broadly expressed on many cell types and can therefore be detected on many types of exosomes, but their expression levels vary depending on the cell type of origin.
Exosome antibodies you can trust
Other commercially available antibodies for tetraspanin proteins CD9, CD63, and CD81 are generally not validated for isolated exosomes and may require tedious optimization for your exosome prep and staining protocol. ExoBrite™ Western Antibody Conjugates were validated to offer bright signal and low background of exosome markers in EV extracts.
Biotium also offers ExoBrite™ EV Membrane Staining Kits and ExoBrite™ Flow Antibodies for detection of isolated EVs and exosomes by flow cytometry. ExoBrite™ EV Membrane Staining Kits may also be combined with antibody staining, for multi-parameter analysis.
ExoBrite™ Western Antibody Conjugates