ExoBrite™ STORM EV Membrane Staining Kits
Fluorescent staining kits developed for direct stochastic optical reconstruction microscopy (dSTORM) imaging of EVs and exosomes.
ExoBrite™ STORM EV Membrane Stains were developed for direct stochastic optical reconstruction microscopy (dSTORM) imaging of extracellular vesicles (EVs) that incorporate a selection of STORM-validated CF® Dyes.
- EV and exosome membrane stains
- Developed for super-resolution imaging by STORM
- Bright signal and low background
- Stain purified or bead-bound exosomes
- Compatible with antibody co-staining
- Available in 4 colors
- ExoBrite™ STORM EV Membrane Stain
- ExoBrite™ Reconstitution Solution
- 1 vial of stain makes 100 uL of 500X staining solution
Extracellular vesicles (EVs), including exosomes, are lipid-bound vesicles that are released from cells. EVs display specific surface proteins and can carry nucleic acids and other cargo, allowing them to transfer biological information between cells in different parts of the body. Therefore, EVs are increasingly studied for their potential use in drug delivery and medical diagnostic applications. Super-resolution microscopy techniques such as direct stochastic optical reconstruction microscopy (dSTORM) push beyond the diffraction limit of traditional light microscopy, allowing single-molecule resolution of subcellular structures, such as the proteins found on EVs.
ExoBrite™ STORM EV Membrane Stains were developed for STORM imaging of EVs and incorporate a selection of our STORM-validated CF® Dyes. Unlike other lipophilic dyes traditionally used to stain EV membranes, ExoBrite™ STORM EV Membrane Stains show little to no background from aggregation in buffer only controls, allowing EVs to be accurately identified. ExoBrite™ 560/585 has been validated for dSTORM on the ONI Nanoimager S Mark II together with fluorescent tetraspanin antibodies, allowing single-EV characterization studies.
|Product||Ex/Em (nm)||Laser Line(s) (nm)||Detection Channel||Size||Catalog Number|
|ExoBrite™ STORM CF®505||505/519||488||FITC||100 Labelings||30115-T|
|ExoBrite™ STORM CF®583R||583/609||555 or 561||Rhodamine or |
|ExoBrite™ STORM CF®647||652/668||633-640||Cy®5||100 Labelings||30117-T|
|ExoBrite™ STORM CF®680||681/698||633-640||Cy®5.5||100 Labelings||30118-T|
Biotium also offers ExoBrite™ EV Membrane Stains and ExoBrite™ Antibody Conjugates
ExoBrite™ staining can be combined with antibody staining (for example, antibodies against tetraspanin proteins CD9, CD63, & CD81), for multi-parameter analysis. Biotium offers a selection of validated ExoBrite™ antibodies optimized for detection of EVs by flow cytometry or western blot. Biotium also offers ExoBrite™ EV Membrane Stains validated for detection of purified or bead-bound EVs by flow cytometry.