Apoptosis Assays
Biotium offers a wide range of fluorescent probes for studying apoptotic cells:
The NucView® Caspase-3 Substrate comes in 3 colors, and measures caspase activity in real time, in live cells. MitoView™ 633 can measure the decrease in mitochondria membrane potential that occurs in early apoptosis. CF® Dye Annexin V conjugates come in many color options, and label PS on the membranes of late apoptotic cells. We also offer kits containing combinations of these probes.
Annexin V Conjugates
29126, 29085, 29001, 29003, 29004, 29005, 29008, 29009, 29010, 29011, 29012, 29013, 29014, 29069, 29045-100uL, 29045-500uL, 29057-100uL, 29083, - 29057-500uLView allHide
Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.
Recombinant Annexin V (Lyophilized)
29089-100ug, 29088-100ug, 29088, 29089, - 29089-5mgView allHide
Recombinant human Annexin V expressed from E. coli as a single, non-glycosylated polypeptide chain. The protein is available with no tag or a C-terminal 6X His tag.
NucView® Caspase-3 Enzyme Substrates
10405-T, 10405, 10407-T, 10407, 10402-T, 10402, 10403-T, 10403, 10406-T, 10406, 10408-T, - 10408View allHide
Fluorescent caspase-3/7 substrates for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or live cell imaging.
Annexin V CF® Dye Conjugates, Azide-Free, Lyophilized
29085R-5ug, 29012R-5ug, 29009R-5ug, 29083R-5ug, 29005R-5ug, 29004R-5ug, 29010R-5ug, 29011R-5ug, 29008R-5ug, 29014R-5ug, 29003R-5ug, - 29069R-5ugView allHide
Preservative-free Annexin V conjugates are compatible with real-time staining of apoptotic cells for live cell imaging, fluorescence microscopy, or flow cytometry.
NucView® 488 & RedDot™ 2 Apoptosis & Necrosis Kit
Homogeneous, no wash assay for detection of caspase-3/7 activity with green fluorescence and dead cell staining with far-red fluorescence, for fluorescence microscopy or flow cytometry.
Ac-DEVD-AMC
Ac-DEVD-AMC is a fluorogenic substrate for caspase 3 and can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader. For real-time detection of caspase-3 activity in intact cells, see our novel NucView®488 Caspase-3 Assay Kit.
5X Annexin V Binding Buffer
Concentrated buffer used for Annexin V staining of cells to monitor apoptosis.
Apoptotic, Necrotic and Healthy Cells Quantitation Kit Plus
A convenient assay for quantifying apoptotic (green), necrotic (red), and total (blue) cells within the same cell population by flow cytometry or fluorescence microscopy.
NucView® 488 & MitoView™ 633 Apoptosis Assay Kit
No wash, homogeneous assay for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using fluorescence microscopy, real-time imaging, or flow cytometry.
Apoptosis and Necrosis Quantitation Kit Plus
A convenient assay for quantifying apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy.
CF®488A Annexin V and PI Apoptosis Kit
This kit contains CF®488A Annexin V to stain apoptotic cells green and propidium iodide (PI) to stain necrotic cells red for fluorescence microscopy or flow cytometry.
CF®488A Annexin V and 7-AAD Apoptosis Kit
This kit contains CF®488A Annexin V to stain apoptotic cells, and 7-AAD to stain necrotic cells for flow cytometry.
NucView® 488 Caspase-3 Assay Kit for Live Cells
30029, - 30029-TView allHide
Green fluorescent caspase-3/7 substrate for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or real-time live cell imaging.
Apoptotic, Necrotic, and Healthy Cells Quantification Kit
A convenient assay for quantifying apoptotic (green), necrotic (red), and total (blue) cells within the same cell population by fluorescence microscopy.
MCB Glutathione Detection Kit
This product is discontinued and no longer available.
Apoptosis and Necrosis Quantification Kit
A convenient assay for staining apoptotic cells with green fluorescence and necrotic cells with red fluorescence for quantitation within the same cell population by flow cytometry or fluorescence microscopy.