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MitoView™ Dyes

MitoView™ dyes are fluorogenic mitochondrial stains for live cells. The dyes rapidly accumulate in mitochondria and can be imaged without washing. They are available with blue, green, far-red, and near-infrared fluorescence.

Product Attributes

Cellular localization

Mitochondria

For live or fixed cells

For live/intact cells

Assay type/options

No-wash staining, Real-time imaging

Cell permeability

Membrane permeant

Potential dependence

Mitochondrial potential-independent, Mitochondrial potential dependent

Apoptosis/viability marker

Mitochondrial potential

Fixation options

Fix before staining (formaldehyde)

Application notes

MitoView™ Green staining is mitochondrial potential-independent, Cells can be fixed with formaldehyde before staining with MitoView™ Green only, Mitochondrial localization of MitoView™ Blue and MitoView™ 720 is potential-dependent, MitoView™ 633 fluorescence is mitochondrial potential-dependent

Colors

Blue, Green, Far-red, Near-infrared

Dye
Size
Catalog #
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Trial size (50 ug)
20 x 50 ug
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Product Description

MitoView™ dyes are fluorogenic mitochondrial stains for live cells. The dyes rapidly accumulate in mitochondria and can be imaged without washing. They are available with blue, green, far-red, and near-infrared fluorescence. Cells can be fixed before staining with MitoView™ Green only, the other MitoView™ dyes are for use in live cells only.

Features

  • Rapidly stain mitochondria in live cells without washing
  • Bright, photostable, and non-toxic
  • Available in five colors with blue, green, far-red, or near-IR emission
  • Monitor mitochondrial membrane potential with MitoView™ 633
  • Potential-independent MitoView™ Green can also stain fixed cells
  • MitoView™ 720 unique near-IR mitochondrial dye

MitoView™ Dyes

ProductAbs/EmDetection channelPotential-dependent?Catalog no.Size
MitoView™ 405398/440 nmDAPIPartial†70070-T50 ug
7007020x50 ug
MitoView™ Green490/523 nmFITC, GFPNo70054-T50 ug
7005420x50 ug
MitoView™ 633622/648 nm*Cy®5, APC*Yes70055-T50 ug
7005520x50 ug
MitoView™ 650644/670Cy®5, APC*Partial†70075-50ug50 ug
7007520x50 ug
MitoView™ 720720/758 nm**Cy®5, Cy®7**Partial†70068-T50 ug
7006820x50 ug
* MitoView™ 633 also has red fluorescence in the Cy®3 channel and is not recommended for use with other red dyes.
**While optimal for Cy®7 settings, MitoView™ 720 is bright enough to be imaged in the Cy®5 channel.
† Dyes localize to the cytoplasm after mitochondrial depolarization, but still retain fluorescence.

• MitoView™ 405 is a blue fluorescent mitochondrial dye with absorbance/emission at 398/440 nm, suitable for detection by confocal microscopy or flow cytometry using settings for DAPI or Pacific Blue®. The dye is membrane permeable and becomes brightly fluorescent upon accumulation in the mitochondrial membrane. Mitochondrial localization is dependent on mitochondrial membrane potential; when membrane potential is disrupted the dye relocalizes to the cytoplasm, but still retains fluorescence. The dye is designed for use in live cells and is not fixable. MitoView™ 405 is a replacement for our original MitoView™ Blue, and has improved photostability.

• MitoView™ Green is a green fluorescent mitochondrial dye with properties similar to those of MitoTracker® Green FM. The dye is non-fluorescent until it partitions into the mitochondrial membrane. The staining relies on mitochondrial mass, not on mitochondria membrane potential. Thus, the dye can be used to stain mitochondria in both live cells and fixed cells. MitoView™ Green is spectrally similar to FITC. Note: MitoView™ Green may be somewhat membrane potential-dependent in yeast cells.

• MitoView™ 633 is a far-red fluorescent mitochondrial dye with absorbance/emission at 622/648 nm. The dye is membrane permeable and becomes brightly fluorescent upon accumulation in the mitochondrial membrane. Staining is dependent on mitochondrial membrane potential, and can be used to monitor mitochondrial membrane potential in intact cells. The dye is designed for use in live cells, and is not fixable. Note: The optimal detection settings for MitoView™ 633 are the same as for Cy®5 and other far-red dyes. However, the dye also has visible red fluorescence and can be imaged using Cy®3 settings as well. As a consequence, the dye cannot be used for two-color imaging with other red probes.

• MitoView™ 650 is a far-red fluorescent mitochondrial dye that is not dependent on mitochondrial membrane potential. The dye has excitation/emission at 644/670 nm for the Cy®5 channel. Unlike MitoView™ 633, it does not bleed into the visible red channel, and so can be combined with red probes for multicolor imaging. The dye fluorescence is not lost after mitochondrial depolarization or fixation, but localization becomes non-specific.

• MitoView™ 720 is a near-infrared mitochondrial dye with absorbance/emission at 720/758 nm. While optimally detected using Cy®7 settings, the dye is bright enough to be imaged in the Cy®5 channel, and can be combined with visible red fluorescent probes. Mitochondrial localization is dependent on mitochondrial membrane potential; when membrane potential is disrupted the dye relocalizes to the cytoplasm but still retains fluorescence.

In addition to MitoView™ dyes, Biotium also offers several classical dyes for measuring mitochondrial membrane potential. These include JC-1, a ratiometric dye that forms red aggregates in healthy cells, which are reduced to the green monomeric state when mitochondrial membrane potential is lost. We also offer TMRM and TMRE, two red fluorescent dyes that are used to quantitatively measure mitochondrial membrane potential using the Nernst equation.

To view our wide selection of other cellular stains, visit our Cellular Stains technology page or see our Cellular Stains Brochure.

Note: MitoView™ Blue (70052) has been discontinued and replaced by the improved MitoView™ 405. The original MitoView™ Blue will be available for purchase as a special order while supplies last. Contact techsupport@biotium.com to inquire about availability.

Cy Dye is a registered trademark of GE Healthcare. MitoTracker is a registered trademark of Thermo Fisher Scientific.

References

Mitoview™ 633 (70055)
1. Cell Death Discovery (2017), doi:10.1038/cddiscovery.2016.71
2. Biochimie (2017), doi: 10.1016/j.biochi.2017.06.004
3. Cell Death and Disease (2017), doi:10.1038/cddis.2017.307
4. Dalton Trans., (2017), doi: 10.1039/C7DT03836D
5. ACS Applied Materials& Interfaces (2018), doi: 10.1021/acsami.8b07917
6. PNAS (2018), www.pnas.org/cgi/doi/10.1073/pnas.1800526115
7. Chem (2018), https://doi.org/10.1016/j.chempr.2018.08.002

Mitoview™ Green (70054)
1. Analytical Chemistry (2018), doi: 10.1021/acs.analchem.8b03035
2. Cell Metabolism (2016), http://dx.doi.org/10.1016/j.cmet.2016.12.021
3. Human Molecular Genetics (2017), https://doi.org/10.1093/hmg/ddx319

 

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