Annexin V fluorescent conjugates can be used to detect apoptotic cells. The human anticoagulant Annexin V is a 35-36 kilodalton, calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labeled Annexin V.
It’s available with the following dyes: CF™350, CF™405M, CF™488A, CF™555, CF™568, CF™594, CF™633, CF™640R, CF™647, CF™660R, CF™680R, FITC, Sulforhodamine 101 (Texas Red®), Biotin, R-PE, and APC.
Texas Red is a registered trademark of Thermo Fisher Scientific.
1. J Immunol Methods 184, 39 (1995).
2. Blood 84, 1415 (1994).
3. Cytometry 43, 134 (2001).
4. FEBS Lett. 203, 99 (1986).
5. Biosci. Rep. 7, 289 (1987).
Download a list of CF dye references.