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Annexin V Conjugates

Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.

Apoptotic Jurkat cell stained with NucView 488 (green) and CF647 Annexin V (magenta).
Catalog #

29001, 29057-500uL, 29057-100uL, 29045-500uL, 29045-100uL, 29069, 29014, 29013, 29012, 29011, 29010, 29009, 29008, 29005, 29004, 29003, 29002, - 29070View allHide

Size

100 uL, 25 ug, 500 uL, 0.5 mL

Conjugation

APC, Biotin, CF350, CF405M, CF488A, CF555, CF568, CF594, CF633, CF640R, CF647, CF660R, CF680R, FITC, R-PE, Sulforhodamine 101 (texas red)

Concentration

50 ug/mL

Conjugation
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100 uL
25 ug
500 uL
0.5 mL
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Product Description

Annexin V fluorescent conjugates can be used to detect apoptotic cells. The human anticoagulant Annexin V is a 35-36 kilodalton, calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labeled Annexin V.

It’s available with the following dyes: CF™350, CF™405M, CF™488A, CF™555, CF™568, CF™594, CF™633, CF™640R, CF™647, CF™660R, CF™680R, FITC, Sulforhodamine 101 (Texas Red®), Biotin, R-PE, and APC.

Also see our Near-IR CF™ Dye Annexin V Conjugates, supplied lyophilized and preservative-free.

We also offer Annexin V in the following apoptosis assay kits:

Texas Red is a registered trademark of Thermo Fisher Scientific.

References

1. J Immunol Methods 184, 39 (1995).
2. Blood 84, 1415 (1994).
3. Cytometry 43, 134 (2001).
4. FEBS Lett. 203, 99 (1986).
5. Biosci. Rep. 7, 289 (1987).

Download a list of CF dye references.

 

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Support & Faq

CF™ Dyes

See our CF™ Dye Quick Reference Table for a list of dyes and summary of their features. Our CF™ Dye Selection Guide has more detailed information on each CF™ dye, and ordering information for our various CF™ dye product lines. You can download CF™ dye normalized absorption and emission data in Excel format.

Category: Mix-n-Stain™ Antibody Labeling Kits, CF™ Dyes

← FAQs

CF initially was an abbreviation for Cyanine-based Fluorescent dyes. These were the first patented CF dyes based on cyanine dye structures. Since then, our CF dye patent portfolio has expanded to include four different fluorescent dye core structures that cover the fluorescence spectrum from UV to NIR.

Category: CF™ Dyes

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The exact chemical structures of CF dyes are currently confidential but will be fully disclosed at a later stage when pending patents become granted. In general terms, the structure of a CF dye may be divided into two parts: a) dye core structure (i.e. the aromatic ring skeleton that defines the dye’s color or absorption/emission wavelengths), and b) core structure-modifying elements. At present, CF dyes bear the core structures of coumarin, pyrene, rhodamine or cyanine dyes. Blue fluorescent CF dyes are based on coumarin or pyrene dye core structure, while green to near-IR CF dyes are based on either cyanine or rhodamine dye core structures. Core structure-modifying elements refer to various chemical attachments to the core structure and are a key aspect of the CF dye invention that makes CF dyes superior to other commercial dyes.

Category: CF™ Dyes

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Apoptosis, Necrosis, and Cell Viability Kits

MTT and XTT are colorimetric based assays, while resazurin can be measured using colorimetric or fluorescence detection. MTT is not a soluble product, so the cells must be lysed to solubilize the formazan salt before absorbance can be measured. XTT and resazurin do not require cell lysis, allowing kinetic monitoring of the same samples at different timepoints.

Category: Apoptosis, Necrosis, and Cell Viability Kits

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Check to make sure your cell densities are in the linear range of the cell viability assay. Too few cells may fall below the limit of detection, and too many cells may saturate the OD. Perform an initial experiment to check the best linear range for your cell type and experimental set-up. You may need to vary the cell density assay incubation time. The kit protocols provide general guidelines and may need to be optimized for your experimental system.

Category: Apoptosis, Necrosis, and Cell Viability Kits

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Resazurin is reduced by cells to the fluorescent product resorufin. Resorufin can be reduced further to a non-fluorescent compound. Therefore the densest wells may have lowest fluorescence due to over-reduction of the substrate. Please see the product information sheet for more details. The kit protocol provides general guidelines and may need to be optimized empirically for your experimental system. You may need to vary cell density or assay incubation time to ensure that your samples fall in the linear range of the kit.

Category: Apoptosis, Necrosis, and Cell Viability Kits

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.