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Annexin V Conjugates

Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.

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Storage Conditions

Store at 2 to 8 °C, Do not freeze, Protect fluorescent conjugates from light

Format

Fluorescent dye & biotin conjugates: Supplied in 10 mM Tris pH 7.5, 1 mM EDTA, 30 mM NaCl, 1 mg/mL BSA, 0.1% sodium azide
R-PE & APC conjugates: Supplied in PBS, 5 mg/mL BSA, 0.05% sodium azide

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Product Description

Annexin V fluorescent conjugates can be used to detect apoptotic cells. The human anticoagulant Annexin V is a 35-36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labeled Annexin V.

CF® Dyes are Biotium’s next-generation fluorescent dyes with advantages in brightness, photostability, and water solubility. We offer Annexin V with a wide selection of CF® dye colors, plus biotin, R-PE, APC, and other dyes.

We also offer Annexin V Conjugates, Azide-Free and Lyophilized in colors ranging from UV to far-red for live cell staining, and Near-IR CF® Dye Annexin V (also lyophilized and preservative-free), compatible with small animal NIR in vivo imaging. Annexin V is also available with other probes in our Apoptotic and Necrotic Staining Kits. See our full selection of Cell Viability and Apoptosis Assays.

Annexin V Conjugates

ProductEx/EmConcentrationSizeCatalog No. / Purchase
Biotin Annexin VN/A50 ug/mL0.5 mLPurchase 29013
CF®350 Annexin V 347/448 nm50 ug/mL0.5 mLPurchase 29012
CF®405M Annexin V 408/452 nm50 ug/mL0.5 mLPurchase 29009
CF®488A Annexin V 490/515 nm50 ug/mL0.5 mLPurchase 29005
CF®555 Annexin V 555/565 nm50 ug/mL0.5 mLPurchase 29004
CF®568 Annexin V 562/583 nm50 ug/mL0.5 mLPurchase 29010
CF®594 Annexin V 593/614 nm50 ug/mL0.5 mLPurchase 29011
CF®633 Annexin V 630/650 nm50 ug/mL0.5 mLPurchase 29008
CF®640R Annexin V 642/662 nm50 ug/mL0.5 mLPurchase 29014
CF®647 Annexin V 650/665 nm50 ug/mL0.5 mLPurchase 29003
CF®660R Annexin V 663/682 nm50 ug/mL0.5 mLPurchase 29069
R-PE Annexin V 496, 546, 565/578 nm5 uL/test100 uLPurchase 29045-100uL
500 uLPurchase 29045-500uL
APC Annexin V 650/660 nm5 uL/test100 uLPurchase 29057-100uL
500 uLPurchase 29057-500uL
FITC Annexin V 496/516 nm50 ug/mL0.5 mLPurchase 29001
Sulforhodamine 101 (Texas Red®) Annexin V 591/608 nm50 ug/mL0.5 mLPurchase 29002
5X Annexin V Binding BufferN/A5X15 mLPurchase 99902

References

1. J Immunol Methods 184, 39 (1995).
2. Blood 84, 1415 (1994).
3. Cytometry 43, 134 (2001).
4. FEBS Lett. 203, 99 (1986).
5. Biosci. Rep. 7, 289 (1987).

Download a list of CF® dye references.

 

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CF® Dyes

See our CF® Dye Quick Reference Table for a list of dyes and summary of their features. Our CF® Dye Selection Guide has more detailed information on each CF® dye, and ordering information for our various CF® dye product lines. You can download CF® dye normalized absorption and emission data in Excel format.

Category: Mix-n-Stain™ Antibody Labeling Kits, CF® Dyes

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CF® initially was an abbreviation for Cyanine-based Fluorescent dyes. These were the first patented CF® dyes based on cyanine dye structures. Since then, our CF® dye patent portfolio has expanded to include four different fluorescent dye core structures that cover the fluorescence spectrum from UV to NIR.

Category: CF® Dyes

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The exact chemical structures of CF® dyes are currently confidential but will be fully disclosed at a later stage when pending patents become granted. In general terms, the structure of a CF® dye may be divided into two parts: a) dye core structure (i.e. the aromatic ring skeleton that defines the dye’s color or absorption/emission wavelengths), and b) core structure-modifying elements. At present, CF® dyes bear the core structures of coumarin, pyrene, rhodamine or cyanine dyes. Blue fluorescent CF® dyes are based on coumarin or pyrene dye core structure, while green to near-IR CF® dyes are based on either cyanine or rhodamine dye core structures. Core structure-modifying elements refer to various chemical attachments to the core structure and are a key aspect of the CF® dye invention that makes CF® dyes superior to other commercial dyes.

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Apoptosis, Necrosis, and Cell Viability Kits

MTT and XTT are colorimetric based assays, while resazurin can be measured using colorimetric or fluorescence detection. MTT is not a soluble product, so the cells must be lysed to solubilize the formazan salt before absorbance can be measured. XTT and resazurin do not require cell lysis, allowing kinetic monitoring of the same samples at different timepoints.

Category: Apoptosis, Necrosis, and Cell Viability Kits

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Check to make sure your cell densities are in the linear range of the cell viability assay. Too few cells may fall below the limit of detection, and too many cells may saturate the OD. Perform an initial experiment to check the best linear range for your cell type and experimental set-up. You may need to vary the cell density assay incubation time. The kit protocols provide general guidelines and may need to be optimized for your experimental system.

Category: Apoptosis, Necrosis, and Cell Viability Kits

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Resazurin is reduced by cells to the fluorescent product resorufin. Resorufin can be reduced further to a non-fluorescent compound. Therefore the densest wells may have lowest fluorescence due to over-reduction of the substrate. Please see the product information sheet for more details. The kit protocol provides general guidelines and may need to be optimized empirically for your experimental system. You may need to vary cell density or assay incubation time to ensure that your samples fall in the linear range of the kit.

Category: Apoptosis, Necrosis, and Cell Viability Kits

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Please request expedited shipping in the Order Notes section if desired. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.