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Annexin V Conjugates

Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.

Certificates of Analysis (COA)

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Storage Conditions

Store at 2 to 8 °C, Do not freeze, Protect fluorescent conjugates from light

Apoptosis/viability marker

Phosphatidylserine/Annexin V

For live or fixed cells

For live/intact cells

Assay type/options

Short term staining (<24h), Endpoint assay, No-wash staining, Real-time imaging, Homogenous assay

Detection method/readout

Fluorescence microscopy, Live cell imaging, Flow cytometry

Format

Fluorescent dye & biotin conjugates: Supplied in 10 mM Tris pH 7.5, 1 mM EDTA, 30 mM NaCl, 1 mg/mL BSA, 0.1% sodium azide
R-PE & APC conjugates: Supplied in PBS, 5 mg/mL BSA, 0.05% sodium azide

Colors

Blue, Green, Orange, Red, Far-red

Fixation options

Fix after staining (formaldehyde)

Conjugation
Size
Catalog #
price
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100 uL
500 uL
0.5 mL
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Product Description

Annexin V fluorescent conjugates can be used to detect apoptotic cells. The human anticoagulant Annexin V is a 35-36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labeled Annexin V.

CF® Dyes are Biotium’s next-generation fluorescent dyes with advantages in brightness, photostability, and water solubility. We offer Annexin V with a wide selection of CF® dye colors, plus biotin, R-PE, APC, and other dyes.

We also offer Annexin V Conjugates, Azide-Free and Lyophilized in colors ranging from UV to far-red for live cell staining, and Near-IR CF® Dye Annexin V (also lyophilized and preservative-free), compatible with small animal NIR in vivo imaging. Annexin V is also available with other probes in our Apoptotic and Necrotic Staining Kits. See our full selection of Cell Viability and Apoptosis Assays.

Annexin V Conjugates

ProductEx/EmConcentrationSizeCatalog No. / Purchase
Biotin Annexin VN/A50 ug/mL0.5 mLPurchase 29013
CF®350 Annexin V 347/448 nm50 ug/mL0.5 mLPurchase 29012
CF®405M Annexin V 408/452 nm50 ug/mL0.5 mLPurchase 29009
CF®488A Annexin V 490/515 nm50 ug/mL0.5 mLPurchase 29005
CF®555 Annexin V 555/565 nm50 ug/mL0.5 mLPurchase 29004
CF®568 Annexin V 562/583 nm50 ug/mL0.5 mLPurchase 29010
CF®594 Annexin V 593/614 nm50 ug/mL0.5 mLPurchase 29011
CF®633 Annexin V 630/650 nm50 ug/mL0.5 mLPurchase 29008
CF®640R Annexin V 642/662 nm50 ug/mL0.5 mLPurchase 29014
CF®647 Annexin V 650/665 nm50 ug/mL0.5 mLPurchase 29003
CF®660R Annexin V 663/682 nm50 ug/mL0.5 mLPurchase 29069
R-PE Annexin V 496, 546, 565/578 nm5 uL/test100 uLPurchase 29045-100uL
500 uLPurchase 29045-500uL
APC Annexin V 650/660 nm5 uL/test100 uLPurchase 29057-100uL
500 uLPurchase 29057-500uL
FITC Annexin V 496/516 nm50 ug/mL0.5 mLPurchase 29001
Sulforhodamine 101 (Texas Red®) Annexin V 591/608 nm50 ug/mL0.5 mLPurchase 29002
5X Annexin V Binding BufferN/A5X15 mLPurchase 99902

References

1. J Immunol Methods 184, 39 (1995).
2. Blood 84, 1415 (1994).
3. Cytometry 43, 134 (2001).
4. FEBS Lett. 203, 99 (1986).
5. Biosci. Rep. 7, 289 (1987).

Download a list of CF® dye references.

 

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Support & Faq

CF® Dyes

See our CF® Dye Quick Reference Table for a list of dyes and summary of their features. Our CF® Dye Selection Guide has more detailed information on each CF® dye, and ordering information for our various CF® dye product lines. You can download CF® dye normalized absorption and emission data in Excel format.

Category: Mix-n-Stain™ Antibody Labeling Kits, CF® Dyes

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CF® initially was an abbreviation for Cyanine-based Fluorescent dyes. These were the first patented CF® dyes based on cyanine dye structures. Since then, our CF® dye patent portfolio has expanded to include four different fluorescent dye core structures that cover the fluorescence spectrum from UV to NIR.

Category: CF® Dyes

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The exact chemical structures of CF® dyes are currently confidential but will be fully disclosed at a later stage when pending patents become granted. In general terms, the structure of a CF® dye may be divided into two parts: a) dye core structure (i.e. the aromatic ring skeleton that defines the dye’s color or absorption/emission wavelengths), and b) core structure-modifying elements. At present, CF® dyes bear the core structures of coumarin, pyrene, rhodamine or cyanine dyes. Blue fluorescent CF® dyes are based on coumarin or pyrene dye core structure, while green to near-IR CF® dyes are based on either cyanine or rhodamine dye core structures. Core structure-modifying elements refer to various chemical attachments to the core structure and are a key aspect of the CF® dye invention that makes CF® dyes superior to other commercial dyes.

Category: CF® Dyes

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Apoptosis, Necrosis, and Cell Viability Kits

Fixation is not recommended after staining with the Viability/Cytotoxicity Assay Kit (cat. # 30027) as the dead cell dye, EthD-III will transfer to all cells and not maintain dead cell-specific staining.

Viability qPCR (vPCR) can be an alternative strategy. Using dead cell-specific dyes, PMA (cat. # 40019) or PMAxx (cat. # 40069) that covalently modify DNA (of only dead cells) after photo-crosslinking, a simple quantitative PCR (qPCR) amplification is used to selectively amplify live-cell DNA. Learn more about the vPCR technology.

Category: Apoptosis, Necrosis, and Cell Viability Kits

← FAQs

The ViaFluor® SE dyes are susceptible to hydrolysis. Ideally the DMSO stock solution should be prepared on the day of use, with the intent that each vial supplied is for single use. If smaller aliquots are desired, it would be best to aliquot the initial DMSO stock, at the time of reconstitution, into single use quantities and store at -20C, protected from light and moisture (desiccated), for up to a month. However, activity may be reduced over time. The dyes should only be added to aqueous buffer immediately before staining. The aqueous solution cannot be stored for reuse.

Category: Apoptosis, Necrosis, and Cell Viability Kits

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MTT and XTT are colorimetric based assays, while resazurin can be measured using colorimetric or fluorescence detection. MTT is not a soluble product, so the cells must be lysed to solubilize the formazan salt before absorbance can be measured. XTT and resazurin do not require cell lysis, allowing kinetic monitoring of the same samples at different timepoints.

Category: Apoptosis, Necrosis, and Cell Viability Kits

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Shipping/Shelf-life

Shipping & Handling Disclaimer: Shipping and handling methods are calculated based on item temperature storage recommendation. Large, multi-item orders may be assessed additional fees. Shipping to Puerto Rico may be assessed additional fees. A Biotium customer service representative will contact you if additional fees are required. For expedited shipping, please request in the Order Notes section of the checkout page. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.