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PMA-Lite™ LED Photolysis Device

PMA-Lite™ LED Photolysis Device is specifically designed for photoactivation of PMAxx™, propidium monoazide (PMA) and other similar dyes.

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Product Description

The PMA-Lite™ LED Photolysis Device can be used to provide continuous illumination for up to 18 samples at a time, for convenient and controlled photoactivation of PMA, PMAxx™, and similar dyes.

Features:

  • Holds up to 18 vials (1.5-2 mL size)
  • Multiple LEDs provide even illumination to the sides and bottoms of all vials
  • Internal fan to maintain temperature ≤37°C
  • Timer for 10, 15, 20 or 30 minutes of photolysis
  • Universal outlet adapter included for customers outside North America
  • Two-year warranty from date of purchase

Specifications:

  • Dimensions (W x D x H): 8.25 x 6.25 x 2.625 in. (21 x 15.9 x 6.7 cm)
  • Weight: 3 lb. 10.7 oz. (1.66 kg)
  • Frequency Range: 50~60 Hz
  • Power Range: 100~240 VAC
  • Internal 120/240 V converter
  • Maximum Power: 60 W
  • LED Output Wavelength: 465-475 nm

PMA for viability PCR

PMA is a photo-reactive dye that binds to dsDNA with high affinity and can be used for the selective detection of viable pathogens by qPCR.  PMAxx™ is a new and improved photoreactive dye for viability PCR. Learn more about PMA and PMAxx™. Each purchase of PMA-Lite™ Photolysis Device includes a free trial size of PMAxx™.


Other PMA-related products:

We also offer the Glo-Plate™ Blue, a flat LED illuminator for viability PCR. While our standard PMA-Lite™ LED photolysis device was designed for use with microcentrifuge tubes, the Glo-Plate™ Blue device allows for more flexible assay set-ups, including multi-well plates for high-throughput assays. The base of the Glo-Plate™ Blue chamber contains blue LED lights topped with a diffuser, allowing for even illumination across samples.

For viability PCR of gram-negative bacteria we highly recommend using our new PMA Enhancer (31038) in conjunction with PMA. PMA Enhancer for Gram Negative Bacteria was designed to improve PMA-mediated discrimination between live and dead gram-negative bacteria. When a sequence from a gram-negative bacteria is amplified by PCR, samples pre-treated with Enhancer show a decrease in the signal from dead cells, with no change in the signal from live cells.

Also see our strain-specific PMA Real-Time PCR Bacterial Viability Kits with validated primers for 5 selected pathogens: M. tuberculosis, S. aureus, MRSA, Salmonella, and E. coli O157:H7. These kits provide everything that you need for the selective detection of your favorite species of live bacteria by real-time PCR.

References

1. PLoS One 12, e0171705. 10.1371/journal.pone.0171705

2. Environ Sci (Camb) 3, 1061-1072. 10.1039/C7EW00184C

3. Lett Appl Microbiol 67, 79-88. 10.1111/lam.12992

4. Front Microbiol 6, 295. 10.3389/fmicb.2015.00295

5. Int J Food Microbiol 244, 74-81. 10.1016/j.ijfoodmicro.2016.12.012

5. Appl Environ Microbiol 85.10.1128/AEM.00334-19

6 .Chemosphere 189, 399-406. 10.1016/j.chemosphere.2017.09.087

7. Food Control. 10.1016/j.foodcont.2015.03.050

8. Appl Microbiol Biotechnol 99, 6451-6462. 10.1007/s00253-015-6715-x

9. Food Microbiol 52, 159-168. 10.1016/j.fm.2015.07.013

Download the full PMA Reference List.

 

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