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PMA Real-Time PCR Bacterial Viability Kit – Listeria monocytogenes (hly)

A kit designed to perform viability PCR on Listeria monocytogenes. It contains a viability dye (PMAxx™ or PMA) and primers to amplify the hly gene.

Product Attributes

Viability dye

PMA, PMAxx™

Storage Conditions

Store at -10 to -35 °C

Assay type/options

Live/dead discrimination, Viability PCR

Detection method/readout

PCR/qPCR, HRM®

Viability dye
Size
Catalog #
price
Qty
200 assays
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Product Description

This kit is designed to perform viability PCR on Listeria monocytogenes. It contains a viability PCR dye (PMA or PMAxx™) and primers to amplify the hly gene.

Features

  • Selectively detect viable Listeria monocytogenes using qPCR
  • Contains primers to amplify the hly gene
  • Works even in complex samples or mixed cultures
  • Sufficient reagents to treat 80 bacterial cultures with PMA or PMAxx™
  • Sufficient reagents for 200 PCR reactions

Kit Components

  • PMA dye or PMAxx™ dye
  • PMA Enhancer for Gram Negative Bacteria
  • Forget-Me-Not™ EvaGreen® qPCR Master Mix
  • ROX reference dye
  • hly primer mix, 5 uM

About this kit

Listeria monocytogenes is a food-borne pathogen that can cause digestive illness and fetal harm. The gene hly encodes the Listeria Lysin O (LLO) protein that is specific for Listeria. PMA-PCR using these primers for viability PCR in Listeria has been published. In addition, these primers have been validated at Biotium for real-time qPCR using Forget-Me-Not™ EvaGreen® qPCR Master Mix.

To learn more about the advantages of determining bacterial viability using viability PCR, visit the Viability PCR Technology Page.

Strain-Specific Bacterial Viability PCR Kits

Bacteria StrainGene nameKit with PMA (catalog #)Kit with PMAxx™ (catalog #)
Salmonella entericainvA3103331033-X
Mycobacterium tuberculosisgroEL231034N/A
Staphylococcus aureusnuc31035N/A
MRSAmecA31036N/A
E. coli O157:H7Z32763103731037-X
E. coliuidA3105031050-X
Listeria monocytogeneshly3105131051-X
Legionella pneumophilamip31053N/A

Don’t see your favorite strain? Let us know at techsupport@biotium.com.

PMAxx technology is covered by granted and/or pending US and international patents.

References

 

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