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PMA Real-Time PCR Bacterial Viability Kit – Listeria monocytogenes (hly)

PMA Real-Time PCR Bacterial Viability Kit – Listeria monocytogenes (hly)

A kit designed to perform viability PCR on Listeria monocytogenes. It contains a viability dye (PMAxx™ or PMA) and primers to amplify the hly gene.

Product Attributes

Viability dye

PMA, PMAxx™
Storage Conditions

Store at -10 to -35 °C
Assay type/options

Live/dead discrimination, Viability PCR
Detection method/readout

PCR/qPCR, HRM®

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Technology

Microbiology Products
Viability PCR

The Full Spectrum Blog

Blog: 12 years later: Viability PCR using PMA and PMAxx™ dyes
Blog: Switching from agar plates to v-PCR for bacteria viability testing – What you need to know
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FAQs

PMA and PMAxx™ for viability PCR
How long can PMA solution be stored if the stock is diluted to 0.2mM solution?

PMA is stable after dilution to 0.2 mM in water as long as it is protected from light and can be stored in the same way as the 20 mM stock solution.


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What’s the difference between EMA, PMA, and PMAxx™?

Viability PCR originally used EMA (ethidium monoazide) to inactivate dead cell DNA. Biotium developed PMA (propidium monoazide) in collaboration with investigators at Montana State University (Nocker et al. 2006). PMA is more selective for dead cells than EMA, and became widely used for selective detection of viable microbes and viruses. PMAxx™ is Biotium’s newest viability PCR dye, designed to be more effective than PMA at eliminating PCR amplification of dead cell DNA. Therefore it provides the best discrimination between live and dead bacteria.

Learn more about PMA and PMAxx™ for viability PCR.


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Should I prepare the PMA stock in DMSO or water?

Both PMA and PMAxx™ are both offered as 20 mM solutions in water. We also offer PMA as a solid, and customers often ask whether it needs to be dissolved in DMSO. While in some publications users have prepared their PMA stock solution in DMSO, this is not necessary. Unlike the older viability dye EMA, which is not soluble in water, PMA and PMAxx™ are very water soluble.


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Viability dye
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Product Description

This kit is designed to perform viability PCR on Listeria monocytogenes. It contains a viability PCR dye (PMA or PMAxx™) and primers to amplify the hly gene.

Features

  • Selectively detect viable Listeria monocytogenes using qPCR
  • Contains primers to amplify the hly gene
  • Works even in complex samples or mixed cultures
  • Sufficient reagents to treat 80 bacterial cultures with PMA or PMAxx™
  • Sufficient reagents for 200 PCR reactions

Kit Components

  • PMA dye or PMAxx™ dye
  • PMA Enhancer for Gram Negative Bacteria
  • Forget-Me-Not™ EvaGreen® qPCR Master Mix
  • ROX reference dye
  • hly primer mix, 5 uM

About this kit

Listeria monocytogenes is a food-borne pathogen that can cause digestive illness and fetal harm. The gene hly encodes the Listeria Lysin O (LLO) protein that is specific for Listeria. PMA-PCR using these primers for viability PCR in Listeria has been published. In addition, these primers have been validated at Biotium for real-time qPCR using Forget-Me-Not™ EvaGreen® qPCR Master Mix.

To learn more about the advantages of determining bacterial viability using viability PCR, visit the Viability PCR Technology Page.

Strain-Specific Bacterial Viability PCR Kits

Bacteria StrainGene nameKit with PMA (catalog #)Kit with PMAxx™ (catalog #)
Salmonella entericainvA3103331033-X
Mycobacterium tuberculosisgroEL231034N/A
Staphylococcus aureusnuc31035N/A
MRSAmecA31036N/A
E. coli O157:H7Z32763103731037-X
E. coliuidA3105031050-X
Listeria monocytogeneshly3105131051-X
Legionella pneumophilamip31053N/A

Don’t see your favorite strain? Let us know at techsupport@biotium.com.

PMAxx technology is covered by granted and/or pending US and international patents.

References

Download PMA and PMAxx™ References and a list of PMA and PMAxx™ Validated Bacterial Strains.

 

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