CytoLiner™ Fixed Cell Membrane Stains
Novel lipophilic fluorescent dyes for selective staining of the plasma membrane in formaldehyde-fixed cells. Available in 5 colors, from blue to near-IR.
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Product Description
CytoLiner™ Fixed Cell Membrane Dyes are novel lipophilic fluorescent dyes developed specifically for selective plasma membrane staining in fixed cells for microscopy. The dyes are engineered for robust and consistent staining of formaldehyde-fixed cells, and are suitable for downstream immunofluorescence staining protocols.
Features
- Robust and consistent staining of plasma membrane in formaldehyde-fixed cells
- Suitable for antibody co-staining
- Compatible with poly-L-lysine coated cultureware
- Available in 5 colors from blue to near-IR
Kit components
- CytoLiner™ Fixed Cell Membrane Dye, 500X in DMSO
- CytoLiner™ Fixed Cell Staining Buffer, 100X
Novel Membrane Dyes Designed for Fixed Cells
While classic lipophilic membrane dyes like DiI can be used to stain formaldehyde-fixed cells, staining can be highly variable due to the poor solubility of the dyes. CytoLiner™ Dyes have optimized chemical structures to balance solubility with lipophilicity to permit selective staining of the plasma membrane in fixed and mildly permeabilized cells. CytoLiner™ staining is compatible with formaldehyde fixation and mild detergent permeabilization before staining. Staining can tolerate blocking agents used during immunofluorescence staining protocols, allowing subsequent staining with antibodies and other probes. The dyes also are compatible with poly-L-lysine coated cultureware and Transwell® permeable filter supports. CytoLiner™ Dyes are available in a selection of 5 colors from blue to near-IR.
Bright and Robust Membrane Staining from Blue to Near-IR
![](https://biotium.com/wp-content/uploads/2024/07/CytoLiner-MCF7-composite-900x599-1.jpg)
Suitable for Co-Staining with Antibodies and Other Probes
![](https://biotium.com/wp-content/uploads/2024/07/CytoLiner-NucSpot-MCF7-composite-900x613-1.jpg)
Find the Right Stain for Your Application
CytoLiner™ Dyes are part of Biotium’s family of unique and innovative cell membrane stains designed for specific applications. For workflows requiring staining live cells before fixation, we recommend our CellBrite® Fix Membrane Stains or MemBrite® Fix Cell Surface Staining Kits. Biotium also offers CF® Dye conjugated lectins, including Concanavalin A (Con A) and Wheat Germ Agglutinin (WGA), for visualizing cell surfaces in live or fixed cells. For longer term monitoring of cell surfaces in live cells, we recommend CellBrite® Steady Dyes. To find the right stain for your application, see our Membrane & Cell Surface Stains Comparison, or download our Membrane & Surface Stains Brochure.
CytoLiner™ Fixed Cell Membrane Stains
Product | Ex/Em | Detection Channels | Size | Catalog Number |
---|---|---|---|---|
CytoLiner™ 410/450 | 406/446 nm | DAPI/Pacific Blue™ | 250 Labelings | 30131-T |
1000 Labelings | 30131 | |||
CytoLiner™ 495/510 | 492/510 nm | FITC | 250 Labelings | 30132-T |
1000 Labelings | 30132 | |||
CytoLiner™ 570/590 | 573/592 nm | Cy®3/TRITC | 250 Labelings | 30133-T |
1000 Labelings | 30133 | |||
CytoLiner™ 650/675 | 647/674 nm | Cy®5 | 250 Labelings | 30134-T |
1000 Labelings | 30134 | |||
CytoLiner™ 680/710 | 682/707 nm | Cy®5.5 | 250 Labelings | 30135-T |
1000 Labelings | 30135 |