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CF® Dye PNA Lectin (Arachis hypogaea)

PNA lectin is a widely used histological fluorescent stain and is labeled with our superior CF® dyes. It is specific for terminal β-galactose and binds preferentially to the commonly occurring structure, galactosyl (β-1,3) N-acetylgalactosamine.

Product Attributes

Colors

Green, Red, Far-red

Cellular localization

Membrane/cell surface

Cell permeability

Membrane impermeant

Fixation options

Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining, Fix before staining (methanol), Fix before staining (formaldehyde)

Conjugation
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1 mg
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Product Description

Arachis hypogaea PNA Lectin is specific for terminal β-galactose and binds preferentially to a commonly occurring structure, galactosyl (β-1,3) N-acetylgalactosamine. PNA conjugates are widely used histological stains.

  • Isolated from peanuts and purified by affinity chromatography
  • Available with green, red, and far-red fluorescence
  • Superior CF® dyes are bright, photostable, and water-soluble

Lectins are proteins or glycoproteins of non-immune origin that agglutinate cells and /or precipitate complex carbohydrates. Lectins are capable of binding glycoproteins even in presence of various detergents. The lectin has a molecular weight of 110 kDa and consists of four identical subunits of approximately 27 kDa each. PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes.

Find the Right Stain for Your Application

PNA and other lectins are carbohydrate binding proteins that recognize specific sugar moieties on glycoproteins.  The presence and distribution of these targets vary between cell types and tissues. As a result, other cell stains or other lectin conjugates, Wheat Germ Agglutinin (WGA) Conjugates and ConA Lectin Conjugates, may produce better staining and may be more appropriate for your cell type. Lectin conjugates can be used to selectively stain the cell surface of live cells, and withstand fixation and permeabilization. When cells are fixed and permeabilized before staining, fluorescent lectins stain both cell surface and organelles in the secretory pathway. Lectins may be toxic or stimulatory to live cells depending on cell type. To find the right stain for your application, see our Membrane & Cell Surface Stains Comparison, or download our Membrane & Surface Stains Brochure. See our Cellular Stains Table for more information on how our dyes stain various organisms.

Superior CF® Dyes

Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

CF Dye PNA Lectin: Product & SKU Summary

ProductConjugationEx/EmSizeCatalog No.
CF®488A PNA Lectin (Arachis hypogaea)CF®488A490/515 nm1 mgPurchase 29060
CF®568 PNA Lectin (Arachis hypogaea)CF®568562/583 nm1 mgPurchase 29061
CF®594 PNA Lectin (Arachis hypogaea)CF®594593/614 nm1 mgPurchase 29062
CF®640R PNA Lectin (Arachis hypogaea)CF®640R642/662 nm1 mgPurchase 29063

 

CF is a registered trademark of Biotium, Inc. Alexa Fluor, Texas Red, and DyLight are registered trademarks of Thermo Fisher Scientific.

References

Download a list of CF® dye references.

 

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