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Wheat Germ Agglutinin (WGA) Conjugates

This product is wheat germ agglutinin (WGA) labeled with Biotium’s CF™ Dyes or HRP. WGA is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine.

Oleic acid treated HeLa cells were fixed then stained with LipidSpot 488 (lipid droplets, green), CF594 WGA (cell surface, red), and Hoechst 33342 (nuclei, blue).
Catalog #

29021, 29024-1, 29025-1, 29026-1, 29027-1, 29028-1, 29029-1, 29059-1, 29064-1, 29077-1, 29077, 29076-1, 29023-1, 29022-1, 29021-1, 29022, 29023, 29024, 29025, 29026, 29027, 29028, 29029, 29059, 29064, 29073, - 29076View allHide

Size

1 mg, 5 x 1 mg

Conjugation

CF350, CF405M, CF405S, CF488A, CF532, CF555, CF568, CF594, CF633, CF640R, CF680, CF680R, CF770, HRP

Conjugation
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Product Description

This product is wheat germ agglutinin (WGA) labeled with Biotium’s CF™ Dyes or HRP. WGA is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine. CF™ Dye WGA conjugates can be used to stain glycoproteins that have these sugar moieties.

WGA can be used as a gram stain, to fluorescently label gram+ bacteria but not gram- bacteria. WGA also binds to the bud scars on budding yeast such as Saccharomyces cerevisiae.

WGA HRP conjugate can be used for Western Blot, ELISA, immunohistochemistry and other standard immunoassay applications.

Wheat Germ Agglutinin (WGA) Conjugates: Product & SKU Summary

ProductConjugationEx/EmSizeSKUPurchase
HRP Wheat Germ Agglutinin (WGA) ConjugatesHRPN/A1 mg29073Purchase 29073
CF350 Wheat Germ Agglutinin (WGA) ConjugatesCF350347/448 nm5 mg29021Purchase 29021
CF350 Wheat Germ Agglutinin (WGA) ConjugatesCF350347/448 nm1 mg29021-1Purchase 29021-1
CF405M Wheat Germ Agglutinin (WGA) ConjugatesCF405M408/452 nm5 mg29028Purchase 29028
CF405M Wheat Germ Agglutinin (WGA) ConjugatesCF405M408/452 nm1 mg29028-1Purchase 29028-1
CF405S Wheat Germ Agglutinin (WGA) ConjugatesCF405S404/431 nm5 mg29027Purchase 29027
CF405S Wheat Germ Agglutinin (WGA) ConjugatesCF405S404/431 nm1 mg29027-1Purchase 29027-1
CF488A Wheat Germ Agglutinin (WGA) ConjugatesCF488A490/515 nm5 mg29022Purchase 29022
CF488A Wheat Germ Agglutinin (WGA) ConjugatesCF488A490/515 nm1 mg29022-1Purchase 29022-1
CF532 Wheat Germ Agglutinin (WGA) ConjugatesCF532527/558 nm5 mg29064Purchase 29064
CF532 Wheat Germ Agglutinin (WGA) ConjugatesCF532527/558 nm1 mg29064-1Purchase 29064-1
CF555 Wheat Germ Agglutinin (WGA) ConjugatesCF555555/565 nm1 mg29076-1Purchase 29076-1
CF555 Wheat Germ Agglutinin (WGA) ConjugatesCF555555/565 nm5 mg29076Purchase 29076
CF568 Wheat Germ Agglutinin (WGA) ConjugatesCF568562/583 nm1 mg29077-1Purchase 29077-1
CF568 Wheat Germ Agglutinin (WGA) ConjugatesCF568562/583 nm5 mg29077Purchase 29077
CF594 Wheat Germ Agglutinin (WGA) ConjugatesCF594593/614 nm5 mg29023Purchase 29023
CF594 Wheat Germ Agglutinin (WGA) ConjugatesCF594593/614 nm1 mg29023-1Purchase 29023-1
CF633 Wheat Germ Agglutinin (WGA) ConjugatesCF633630/650 nm5 mg29024Purchase 29024
CF633 Wheat Germ Agglutinin (WGA) ConjugatesCF633630/650 nm1 mg29024-1Purchase 29024-1
CF640R Wheat Germ Agglutinin (WGA) ConjugatesCF640R642/662 nm5 mg29026Purchase 29026
CF640R Wheat Germ Agglutinin (WGA) ConjugatesCF640R642/662 nm1 mg29026-1Purchase 29026-1
CF680 Wheat Germ Agglutinin (WGA) ConjugatesCF680681/698 nm5 mg29029Purchase 29029
CF680 Wheat Germ Agglutinin (WGA) ConjugatesCF680681/698 nm1 mg29029-1Purchase 29029-1
CF680R Wheat Germ Agglutinin (WGA) ConjugatesCF680R680/701 nm5 mg29025Purchase 29025
CF680R Wheat Germ Agglutinin (WGA) ConjugatesCF680R680/701 nm1 mg29025-1Purchase 29025-1
CF770 Wheat Germ Agglutinin (WGA) ConjugatesCF770770/797 nm5 mg29059Purchase 29059
CF770 Wheat Germ Agglutinin (WGA) ConjugatesCF770770/797 nm1 mg29059-1Purchase 29059-1

References

Download a list of CF dye references.

 

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Support & Faq

Product shelf life, solubility, shipping and storage, and stability

Bioscience kits
The guaranteed shelf life from date of receipt for bioscience kits is listed on the product information sheet. Some kits have an expiration date printed on the kit box label, this is the guaranteed shelf life date calculated from the day that the product shipped from our facility. Kits often are functional for significantly longer than the guaranteed shelf life. If you have an older kit in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the kit still works for your application before processing a large number of samples or precious samples.

Antibodies and other conjugates
The guaranteed shelf life from date of receipt for antibodies and conjugates is listed on the product information sheet. Antibodies and other conjugates often are functional for significantly longer than the guaranteed shelf life. If you have an older conjugate in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples.

For lyophilized antibodies, we recommend reconstituting the antibody with glycerol and antimicrobial preservative like sodium azide for the longest shelf life (note that sodium azide is not compatible with HRP-conjugates).

Chemicals, dyes, and gel stains
Biotium guarantees the stability of chemicals, dyes, and gel stains for at least a year from the date you receive the product. However, the majority of these products are highly stable for many years, as long as they are stored as recommended. Storage conditions can be found on the product information sheet or product safety and data sheet, material safety data sheet, and on the product label. Fluorescent compounds should be protected from light for long term storage.

If you have a Biotium compound that has been in storage for longer than one year that you wish to use, we recommend performing a small scale positive control experiment to confirm that the compound still works for your application before processing a large number of samples or precious samples.

Expiration date based on date of manufacture (DOM)
If your institution requires you to document expiration date based on date of manufacture for reagents, please contact techsupport@biotium.com for assistance.

Chemical products with special stability considerations:

Esters

Ester compounds include the following:
• Succinimidyl esters (SE, also known as NHS esters), such as our amine-reactive dyes
• Acetoxymethyl esters (AM esters) such as our membrane-permeable ion indicator dyes
• Diacetate-modified dyes, like ViaFluor™ 405, CFDA, and CFDA-SE cell viability/cell proliferation dyes

Ester dyes are stable in solid form as long as they are protected from light and moisture. Esters are not stable in aqueous solution. Concentrated stock solutions should be prepared in anhydrous DMSO (see Biotium catalog no. 90082). Stock solutions in anhydrous DMSO can be stored desiccated at -20°C for one month or longer. Esters should be diluted in aqueous solution immediately before use. Succinimidyl esters (SE) should be dissolved in a solution that is free of amine-containing compounds like Tris, glycine, or protein, which will react with the SE functional group. AM esters and diacetate compounds should be dissolved in a solution that is free of serum, because serum could contain esterases that would hydrolyze the compound.

A note on CF™ dye succinimidyl ester stability
Succinimidyl esters are generally susceptible to hydrolysis, which can result in lower labeling efficiency. Heavily sulfonated dyes, such as the Alexa Fluor® dyes, DyLight® dyes and IRDyes® are particularly hygroscopic, worsening the hydrolysis problem. For example, the percent of active Alexa Fluor® 488 succinimidyl ester (SE) could be well below 50% by the time of application (according to the manufacturer’s product datasheet). In a number of Alexa Fluor® SE reactive dyes, the SE group is derived from an aromatic carboxylic acid, while in all of Biotium’s CF™ dyes the SE group is prepared from an aliphatic carboxylic acid. This structural difference reduces the susceptibility of CF™ dye SE reactive groups to hydrolysis, resulting in relatively stable reactive dyes with consistently higher labeling efficiency compared to other SE derivatives of other fluorescent dyes.

Maleimides, MTS and thiosulfate dyes
Like the succinimidyl ester dyes, these dyes are also susceptible to hydrolysis, although generally to a much lower degree. Thus, for long term storage, anhydrous DMSO is recommended for making stock solutions.

Other reactive dyes
Amines, aminooxy (also known as oxylamine), hydrazide, azide, alkyne, BCN, and tyramide reactive dyes, as well as dye free acids, are generally stable in aqueous solution when stored at -20°C for 6-12 months or longer, as long as no compounds are present that may react with the dye’s functional group. See the product information sheets for specific reactive dyes more information.

Coelenterazines and D-luciferin

Coelenterazines are stable in solid form when stored as recommended; they are not stable in aqueous solution. Concentrated coelenterazine stock solutions (typically 1-100 mg/mL) should be prepared in ethanol or methanol; do not use DMSO or DMF to dissolve coelenterazines, because these solvents will oxidize the compounds. Ethanol or methanol stocks of coelenterazine can be stored at -20°C or below for six months or longer; alcohol stocks may evaporate during storage, so use tightly sealing screw cap vials and wrap the vials with Parafilm for long term storage. Propylene glycol also can be used as a solvent to minimize evaporation. If the solvent evaporates, the coelenterazine will still be present in the vial, so note the volume in the vial prior to storage so that you can adjust the solvent volume to correct for evaporation if needed. Prepare working solutions in aqueous buffers immediately before use. Coelenterazines are stable for up to five hours in aqueous solution.

Aquaphile™ coelenterazines are water soluble formulations of coelenterazines. They are stable in solid form when stored as recommended. Aquaphile™ coelenterazines should be dissolved in aqueous solution immediately before use. They are stable for up to five hours in aqueous solution.

Note that coelenterazines are predominantly yellow solids, but may contain dark red or brown flecks. This does not affect product stability or performance. If your coelenterazine is uniformly brown, then it is oxidized and needs to be replaced.

D-luciferin is stable in solid form and as a concentrated stock solution when stored as recommended; it is not stable at dilute working concentrations in aqueous solution. Prepare concentrated D-luciferin stock solutions (typically 1-100 mg/mL) in water, and store in aliquots at -20°C or below for six months or longer. Prepare working solutions immediately before use.

Category: Mix-n-Stain™ Antibody Labeling Kits, DNA Quantitation Kits, NucView™ Caspase 3 Enzyme Substrates, Luciferase Assays, CF™ Dyes, Product shelf life, solubility, shipping and storage, and stability

← FAQs

Many of our solid compounds are packaged by lyophilization, in which case they usually do not appear as fluffy powders, but form a film or coating on the sides of the vial. Simply add the appropriate volume of the recommended solvent to the vial to make the desired concentration stock solution, and swirl or gently vortex to mix. Make sure the solvent comes in contact with the inside walls of the vial to fully recover the product.

Category: Product shelf life, solubility, shipping and storage, and stability

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Most of our products are stable at room temperature for many days, but we recommend storage at 4°C or -20°C to prolong shelf life. In the case of many of our aqueous dye solutions, the compounds are very stable at room temperature, but we recommend cold storage to prevent the growth of mold or other microbes over time. Therefore, to save on shipping costs, products with recommended storage at 4°C or -20°C may ship at ambient temperature without affecting product performance. When you receive the product, place it under the recommended storage conditions.

Category: Mix-n-Stain™ Antibody Labeling Kits, DNA Quantitation Kits, NucView™ Caspase 3 Enzyme Substrates, Luciferase Assays, CF™ Dyes, Product shelf life, solubility, shipping and storage, and stability

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.