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Wheat Germ Agglutinin (WGA) Conjugates

This product is wheat germ agglutinin (WGA) labeled with Biotium’s CF® Dyes or HRP. WGA is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine.

Product Attributes

Cellular localization

Membrane/cell surface

For live or fixed cells

For live/intact cells, For fixed cells

Cell permeability

Membrane impermeant

Fixation options

Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining, Fix before staining (methanol), Fix before staining (formaldehyde)

Colors

Blue, Green, Orange, Red, Far-red, Near-infrared

Conjugation
Size
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1 mg
5 x 1 mg
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Product Description

This product is wheat germ agglutinin (WGA) labeled with Biotium’s CF® Dyes or HRP. WGA is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine. CF® Dye WGA conjugates can be used to stain glycoproteins that have these sugar moieties. WGA also can be used to stain the cell surface of live or fixed cells, but this can vary by cell type.

WGA can be used as a gram stain to fluorescently label gram+ bacteria but not gram- bacteria. WGA also binds to the bud scars on budding yeast such as Saccharomyces cerevisiae. See our Cellular Stains Table for more information on how our dyes stain various organisms.

WGA HRP conjugate can be used for Western Blot, ELISA, immunohistochemistry and other standard immunoassay applications.

ProductConjugationEx/EmSizeCatalog No.Purchase
HRP WGAHRPN/A1 mg29073Purchase 29073
CF®350 WGACF®350347/448 nm1 mg29021-1Purchase 29021-1
5 mg29021Purchase 29021
CF®405S WGACF®405S404/431 nm1 mg29027-1Purchase 29027-1
5 mg29027Purchase 29027
CF®405M WGACF®405M408/452 nm1 mg29028-1Purchase 29028-1
5 mg29028Purchase 29028
CF®488A WGACF®488A490/515 nm1 mg29022-1Purchase 29022-1
5 mg29022Purchase 29022
CF®532 WGACF®532527/558 nm1 mg29064-1Purchase 29064-1
5 mg29064Purchase 29064
CF®555 WGACF®555555/565 nm5 mg29076Purchase 29076
1 mg29076-1Purchase 29076-1
CF®568 WGACF®568562/583 nm5 mg29077Purchase 29077
1 mg29077-1Purchase 29077-1
CF®594 WGACF®594593/614 nm1 mg29023-1Purchase 29023-1
5 mg29023Purchase 29023
CF®633 WGACF®633630/650 nm1 mg29024-1Purchase 29024-1
5 mg29024Purchase 29024
CF®640R WGACF®640R642/662 nm1 mg29026-1Purchase 29026-1
5 mg29026Purchase 29026
CF®680 WGACF®680681/698 nm1 mg29029-1Purchase 29029-1
5 mg29029Purchase 29029
CF®680R WGA CF®680R680/701 nm1 mg29025-1Purchase 29025-1
5 mg29025Purchase 29025
CF®770 WGACF®770770/797 nm1 mg29059-1Purchase 29059-1
5 mg29059Purchase 29059

References

Download a list of CF® dye references.

 

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