Wheat Germ Agglutinin (WGA) Conjugates
A wheat germ agglutinin (WGA) lectin labeled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine and stains yeast bud scars as well as the cell membrane of gram+ bacteria and mammalian cells.
Wheat germ agglutinin (WGA) is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram bacteria and mammalian cells.
- Stain yeast bud scars as well as the cell membrane of gram bacteria and mammalian cells or tissues
- Suitable for Western Blot, ELISA, immunohistochemistry and other standard immunoassay applications
- A retrograde or anterograde neuronal tracer
- Withstands fixation and permeabilization
- Choice of 13 CF® dyes from UV to near-infrared or HRP
- Superior CF® dyes are bright, photostable, and water-soluble
WGA is commonly used to label glycoproteins for imaging of the plasma membrane in live or fixed cells, for staining of tissue sections, or for western blotting. WGA can be used as a gram stain to fluorescently label gram bacteria but not gram- bacteria. WGA also binds to the bud scars on budding yeast such as Saccharomyces cerevisiae.
Find the Right Stain for Your Application
WGA and other lectins are carbohydrate binding proteins that recognize specific sugar moieties on glycoproteins. The presence and distribution of these targets vary between cell types and tissues. As a result, other cell surface stains or other lectin conjugates, Concanavalin A (Con A) Conjugates and PNA Lectin Conjugates, may produce better surface staining and may be more appropriate for your cell type. Lectin conjugates can be used to selectively stain the cell surface of live cells, and withstand fixation and permeabilization. When cells are fixed and permeabilized before staining, fluorescent lectins stain both cell surface and organelles in the secretory pathway. Lectins may be toxic or stimulatory to live cells depending on cell type. To find the right stain for your application, see our Membrane & Cell Surface Stains Comparison, or download our Membrane & Surface Stains Brochure. See our Cellular Stains Table for more information on how our dyes stain various organisms.
Superior CF® Dyes
Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.
Note: Conjugates of blue-fluorescent dyes like CF®350, CF®405S and CF®405M are not recommended for detecting low abundance targets and may be challenging to use in tissue specimens. Blue dyes have lower fluorescence and photostability, and cells and tissue have high autofluorescence in blue wavelengths, resulting in lower signal to noise compared to other colors.
Wheat Germ Agglutinin Conjugates
|HRP WGA||HRP||N/A||1 mg||29073||Purchase 29073|
|CF®350 WGA||CF®350||347/448 nm||1 mg||29021-1||Purchase 29021-1|
|5 mg||29021||Purchase 29021|
|CF®405S WGA||CF®405S||404/431 nm||1 mg||29027-1||Purchase 29027-1|
|5 mg||29027||Purchase 29027|
|CF®405M WGA||CF®405M||408/452 nm||1 mg||29028-1||Purchase 29028-1|
|5 mg||29028||Purchase 29028|
|CF®488A WGA||CF®488A||490/515 nm||1 mg||29022-1||Purchase 29022-1|
|5 mg||29022||Purchase 29022|
|CF®532 WGA||CF®532||527/558 nm||1 mg||29064-1||Purchase 29064-1|
|5 mg||29064||Purchase 29064|
|CF®555 WGA||CF®555||555/565 nm||5 mg||29076||Purchase 29076|
|1 mg||29076-1||Purchase 29076-1|
|CF®568 WGA||CF®568||562/583 nm||5 mg||29077||Purchase 29077|
|1 mg||29077-1||Purchase 29077-1|
|CF®594 WGA||CF®594||593/614 nm||1 mg||29023-1||Purchase 29023-1|
|5 mg||29023||Purchase 29023|
|CF®633 WGA||CF®633||630/650 nm||1 mg||29024-1||Purchase 29024-1|
|5 mg||29024||Purchase 29024|
|CF®640R WGA||CF®640R||642/662 nm||1 mg||29026-1||Purchase 29026-1|
|5 mg||29026||Purchase 29026|
|CF®680 WGA||CF®680||681/698 nm||1 mg||29029-1||Purchase 29029-1|
|5 mg||29029||Purchase 29029|
|CF®680R WGA||CF®680R||680/701 nm||1 mg||29025-1||Purchase 29025-1|
|5 mg||29025||Purchase 29025|
|CF®770 WGA||CF®770||770/797 nm||1 mg||29059-1||Purchase 29059-1|
|5 mg||29059||Purchase 29059|
CF® Dye WGA in bacteria
1. BMC Microbiol. (2014), DOI: 10.1186/1471-2180-14-56
CF® Dye WGA in fungi
2. Eur J Plant Pathol. (2017), DOI: 10.1007/s10658-017-1226-5
3. Sci Rep. (2017), DOI: 10.1038/s41598-017-16401-6.
CF® Dye WGA in cells & tissues
4. J Tissue Eng. (2012), DOI: 10.1177/2041731412455354
5. Nanotechnology (2013), DOI: 10.1088/0957-4484/24/28/285101
6. BMC Biol. (2016), DOI: 10.1186/s12915-016-0332-8
7. Macromol. Biosci. (2016), DOI: 10.1002/mabi.201600333
8. PLoS One (2016), DOI: 10.1371/journal.pone.0156456
9. Sci Rep. (2017), DOI: 10.1038/srep42941
10. MBio. (2017), DOI: 10.1128/mBio.02030-16
11. FEBS J. (2019) DOI: 10.1111/febs.15088
12. Cell Physiol Biochem.(2019) DOI: 10.33594/000000070