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Concanavalin A (Con A) CF® Dye Conjugates

A lectin labeled with our superior fluorescent CF® dyes is an effective and widely used cell surface stain for yeast, fungi, and mammalian cells.

Product Attributes

Cellular localization

Membrane/cell surface

For live or fixed cells

For fixed cells, For live/intact cells

Cell permeability

Membrane impermeant

Fixation options

Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining, Fix before staining (methanol), Fix before staining (formaldehyde)

Colors

Blue, Green, Orange, Red, Far-red, Near-infrared

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

Concanavalin A (Con A) is a widely used lectin that selectively binds to the glycoproteins, a-mannopyranosyl and a-glucopyranosy. These are commonly found in the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues.

  • Stain the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues
  • Detect glycoconjugates in microscopy and flow cytometry
  • Stain glycoproteins in gels
  • Withstands fixation and permeabilization
  • Choice of 10 CF® dyes from UV to near-infrared
  • Superior CF® dyes are bright, photostable, and water-soluble

Lectins are also versatile probes for detecting glycoconjugates in microscopy and flow cytometric applications and for gel staining of glycoproteins. In neutral and alkaline solutions, Con A exists as a tetramer with a molecular weight of approximately 104 kDa. In acidic solutions (pH below 5.0), Con A exists as a dimer. Con A can be used to selectively stain the cell surface of live cells, and withstand fixation and permeabilization. When cells are fixed and permeabilized before staining, fluorescent lectins stain both cell surface and organelles in the secretory pathway.

Find the Right Stain for Your Application

Con A and other lectins are carbohydrate binding proteins that recognize specific sugar moieties on glycoproteins.  The presence and distribution of these targets vary between cell types and tissues. As a result, other cell surface stains or other lectin conjugates, Wheat Germ Agglutinin (WGA) Conjugates and PNA Lectin Conjugates, may produce better surface staining and may be more appropriate for your cell type. Lectin conjugates can be used to selectively stain the cell surface of live cells, and withstand fixation and permeabilization. When cells are fixed and permeabilized before staining, fluorescent lectins stain both cell surface and organelles in the secretory pathway. Lectins may be toxic or stimulatory to live cells depending on cell type. To find the right stain for your application, see our Membrane & Cell Surface Stains Comparison, or download our Membrane & Surface Stains Brochure. See our Cellular Stains Table for more information on how our dyes stain various organisms.

Superior CF® Dyes

Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

Concanavalin A Dye Conjugates

ProductConjugationEx/EmSizeCatalog No.Purchase
CF®350 Concanavalin A (Con A) CF®350347/448 nm5 mg29015Purchase 29015
CF®405S Concanavalin A (Con A) CF®405S404/431 nm5 mg29075Purchase 29075
CF®405M Concanavalin A (Con A) CF®405M408/452 nm5 mg29074Purchase 29074
CF®488A Concanavalin A (Con A) CF®488A490/515 nm5 mg29016Purchase 29016
CF®594 Concanavalin A (Con A) CF®594593/614 nm5 mg29017Purchase 29017
CF®633 Concanavalin A (Con A) CF®633630/650 nm5 mg29018Purchase 29018
CF®640R Concanavalin A (Con A) CF®640R642/662 nm5 mg29019Purchase 29019
CF®680 Concanavalin A (Con A) CF®680681/698 nm5 mg29020Purchase 29020
CF®750 Concanavalin A (Con A) CF®750755/777 nm5 mg29080Purchase 29080
CF®770 Concanavalin A (Con A) CF®770770/797 nm5 mg29058Purchase 29058

 

CF is a registered trademark of Biotium, Inc. Alexa Fluor, Texas Red, and DyLight are registered trademarks of Thermo Fisher Scientific.

References

CF®680 ConA
Acta Histochem (2019), DOI: 10.1016/j.acthis.2018.11.006.

Download a list of CF® dye references.

 

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