Concanavalin A (Con A) is a widely used lectin that selectively binds to the glycoproteins, a-mannopyranosyl and a-glucopyranosy. These are commonly found in the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues.
- Stain the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues
- Detect glycoconjugates in microscopy and flow cytometry
- Stain glycoproteins in gels
- Withstands fixation and permeabilization
- Choice of 10 CF® dyes from UV to near-infrared
- Superior CF® dyes are bright, photostable, and water-soluble
Lectins are also versatile probes for detecting glycoconjugates in microscopy and flow cytometric applications and for gel staining of glycoproteins. In neutral and alkaline solutions, Con A exists as a tetramer with a molecular weight of approximately 104 kDa. In acidic solutions (pH below 5.0), Con A exists as a dimer. Con A can be used to selectively stain the cell surface of live cells, and withstand fixation and permeabilization. When cells are fixed and permeabilized before staining, fluorescent lectins stain both cell surface and organelles in the secretory pathway.
Find the Right Stain for Your Application
Con A and other lectins are carbohydrate binding proteins that recognize specific sugar moieties on glycoproteins. The presence and distribution of these targets vary between cell types and tissues. As a result, other cell surface stains or other lectin conjugates, Wheat Germ Agglutinin (WGA) Conjugates and PNA Lectin Conjugates, may produce better surface staining and may be more appropriate for your cell type. Lectin conjugates can be used to selectively stain the cell surface of live cells, and withstand fixation and permeabilization. When cells are fixed and permeabilized before staining, fluorescent lectins stain both cell surface and organelles in the secretory pathway. Lectins may be toxic or stimulatory to live cells depending on cell type. To find the right stain for your application, see our Membrane & Cell Surface Stains Comparison, or download our Membrane & Surface Stains Brochure. See our Cellular Stains Table for more information on how our dyes stain various organisms.
Superior CF® Dyes
Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.
Note: Conjugates of blue-fluorescent dyes like CF®350, CF®405S and CF®405M are not recommended for detecting low abundance targets and may be challenging to use in tissue specimens. Blue dyes have lower fluorescence and photostability, and cells and tissue have high autofluorescence in blue wavelengths, resulting in lower signal to noise compared to other colors.
Concanavalin A Dye Conjugates
|CF®350 Concanavalin A (Con A)
|CF®405S Concanavalin A (Con A)
|CF®405M Concanavalin A (Con A)
|CF®488A Concanavalin A (Con A)
|CF®594 Concanavalin A (Con A)
|CF®633 Concanavalin A (Con A)
|CF®640R Concanavalin A (Con A)
|CF®680 Concanavalin A (Con A)
|CF®750 Concanavalin A (Con A)
|CF®770 Concanavalin A (Con A)
CF is a registered trademark of Biotium, Inc. Alexa Fluor, Texas Red, and DyLight are registered trademarks of Thermo Fisher Scientific.