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MemBrite™ Fix Cell Surface Staining Kits

MemBrite™ Fix Cell Surface Staining Kits provide a convenient way to visualize cell boundaries in multicolor staining experiments. Staining is rapid and uniform, with a wide choice of colors. MemBrite™ Fix dyes covalently label the surface of live cells. The staining withstands fixation and permeabilization for subsequent immunofluorescence staining.

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Product Description

MemBrite™ Fix Features

  • Unique kits allow you to conveniently image cell boundaries
  • Rapid, uniform live cell surface staining
  • Covalent labeling can be fixed & permeabilized for IF staining
  • Stain yeast and gram-positive bacteria
  • Choose from eight bright & photostable dye colors from blue to near-IR
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MemBrite™ Fix Cell Surface Staining Kits are designed for covalently staining the surface of live cells to conveniently visualize cell boundaries in immunofluorescence experiments. Traditional membrane dyes like DiO, DiI, PKH, Vybrant®, and CellMask™ are lipophilic dyes that incorporate into cell membranes. They tend to label cell unevenly due to their low solubility, and they cannot withstand detergent permeabilization or alcohol fixation. MemBrite™ Fix are new covalent cell surface stains that react irreversibly with cell surface proteins for staining that can withstand formaldehyde or alcohol fixation, and detergent permeabilization. Unlike lectins such as WGA, which bind specific targets that may vary between cell types, MemBrite™ Fix dyes react widely with cell surface proteins. MemBrite™ Fix staining is rapid and non-toxic to cells, and because MemBrite™ Fix dyes are highly water soluble, they stain cells much more evenly than lipophilic membrane dyes. MemBrite™ Fix staining kits also can be used to stain yeast and gram-positive bacteria, but not gram-negative bacteria.

MemBrite™ Fix Staining Kits belong to Biotium’s line of novel cell surface stains that include CellBrite™ Fix Membrane Stains. CellBrite™ Fix Membrane Stains are fluorogenic dyes that rapidly accumulate in the plasma membrane, where they react covalently with the cell surface. CellBrite™ Fix stains require only a single staining step compared to MemBrite™ Fix staining, which is a two-step protocol. On the other hand, MemBrite™ Fix dyes are available with a wider selection of colors. MemBrite™ dyes do not associate with lipids in membranes, and consequently have lower cytoplasmic background after detergent permeabilization compared to CellBrite™ Fix.

To find the right stain for your application, see our Membrane & Cell Surface Stains Comparison, or download our Membrane & Surface Stains Brochure.

MemBrite™ Fix Cell Surface Staining Kits

Catalog numberSize*Dye Abs/Em (nm)**Spectrally similar to
30092-T100 reactionsMemBrite™ Fix 405/430Alexa Fluor® 405, CellBrite™ Blue
30092500 reactions
30093-T100 reactionsMemBrite™ Fix 488/515FITC, Alexa Fluor® 488; DiO; CellBrite™ Green
30093500 reactions
30094-T100 reactionsMemBrite™ Fix 543/560TAMRA, Cy®3, Alexa Fluor® 546, DiI, CellBrite™ Orange
30094500 reactions
30095-T100 reactionsMemBrite™ Fix 568/580Alexa Fluor® 568, Rhodamine Red, DiI, CellBrite™ Orange
30095500 reactions
30096-T100 reactionsMemBrite™ Fix 594/615Texas Red®, Alexa Fluor® 594
30096500 reactions
30097-T100 reactionsMemBrite™ Fix 640/660Cy®5, Alexa Fluor® 647, DiD, CellBrite™ Red
30097500 reactions
30098-T100 reactionsMemBrite™ Fix 660/680Alexa Fluor® 660
30098500 reactions
30099-T100 reactionsMemBrite™ Fix 680/700Cy®5.5, Alexa Fluor® 680, IRDye® 680LT, CellBrite™ NIR 680
30099500 reactions
*Kit size based on 200 uL reaction volume, actual number of reactions depends on sample size
**MemBrite Fix dyes are named for their abs/em maxima. Download the product information sheet for spectra.
Alexa Fluor & Texas Red are registered trademarks of Thermo Fisher Scientific; Cy Dye is a registered trademark of GE Healthcare; IRDye is a registered trademark of LI-COR Bioscience.
Vybrant is a registered trademark and CellMask is a trademark of Thermo Fisher Scientific.
 

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Support & Faq

CellBrite™ & MemBrite™ Membrane & Cell Surface Stains

CellBrite™ Cytoplasmic Membrane Stains are lipophilic dyes for simple, non-toxic, stable labeling of membranes in live or fixed cells. Cells can be fixed with formaldehyde before or after CellBrite™ staining. But the staining has poor tolerance for permeabilization or methanol fixation, so CellBrite™ staining is not easily combined with intracellular immunofluorescence (IF) staining. The dyes also do not stain bacteria or yeast. CellBrite™ NIR dyes are CellBrite™ dyes with near-infrared fluorescence compatible with small animal NIR imaging systems.

CellBrite™ Fix and MemBrite™ Fix stains were developed to overcome some of these shortcomings. They are novel covalent stains that can be fixed and permeabilized for IF staining. CellBrite™ Fix Membrane Stains are fluorogenic reactive membrane dyes that rapidly accumulate at the plasma membrane. When they incorporate into lipids, they become fluorescent, and at the same time react covalently with membrane proteins for stable labeling. Staining takes only 15 minutes in a single step with no wash. CellBrite™ Fix stains mammalian cells, yeast, and bacteria.

MemBrite™ Fix Cell Surface Stains do not bind lipids, but label cell surface proteins. MemBrite™ Fix requires a two-step staining protocol with washing, but offers a more extensive choice of dye colors than CellBrite™ Fix. MemBrite™ Fix also can be used to stain yeast. But unlike original CellBrite™ dyes and lectins, CellBrite™ Fix and MemBrite™ Fix cannot be used on cells that are already fixed.

To select a dye that’s right for your application, see our Membrane and Cell Surface Stains Comparison, or download our Membrane & Surface Stains Brochure.

Category: CellBrite™ & MemBrite™ Membrane & Cell Surface Stains

← FAQs

Original CellBrite™ Cytoplasmic Membrane Stains are lipophilic carbocyanine dyes. These dyes undergo an increase in fluorescence when they insert into lipid bilayers. Lipophilic carbocyanine dyes stably label the plasma membrane and other intracellular membranes of cells. They also can be used to stain fixed cells or artificial lipid bilayers.

CellBrite™ Fix Membrane Stains are fluorogenic membrane dyes that react covalently with proteins on the cell surface. They have better water solubility than original CellBrite™ dyes and as a result generally give more even staining of the cell surface compared to original CellBrite™ dyes. CellBrite™ Fix are well retained after fixation and permeabiliation or methanol fixation. However, CellBrite™ Fix dyes cannot be used to stain the plasma membrane of cells after they are already fixed (the dyes stain intracellular membranes of fixed cells).

For all membrane stains, if cells are returned to culture after staining, membrane internalization will occur over time, resulting in predominantly intracellular staining.

Category: CellBrite™ & MemBrite™ Membrane & Cell Surface Stains

← FAQs

Lipophilic carbocyanine dyes like our original CellBrite™ Cytoplasmic Membrane Stains have been used to stain neuronal cells in culture for several weeks, and in vivo for up to a year. The dyes do not appreciably affect cell viability, and do not readily transfer between cells with intact membranes, allowing cell migration and tracking studies in mixed populations. Over time, the dyes will be internalized and become intracellular as membranes turn over. Long term stability of labeling may vary between cell types, depending on rates of membrane turnover or cell division.

CellBrite™ Fix Membrane Stains label the cell surface covalently. Like original CellBrite™ dyes, they are non-toxic and do not readily transfer between cells, but will be internalized and become intracellular as membranes turn over.

Category: CellBrite™ & MemBrite™ Membrane & Cell Surface Stains

← FAQs

← FAQs

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Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Please request expedited shipping in the Order Notes section if desired. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.