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NucSpot® Live Cell Nuclear Stains

No-wash, fluorescent DNA nuclear stains dyes with low toxicity for long-term live cell imaging or fixed cell staining. Choose green or far-red fluorescence.

Product Attributes

Dye

NucSpot® Live 488, NucSpot® Live 650

Cellular localization

Nucleus

For live or fixed cells

For fixed cells, For live/intact cells

Assay type/options

Long term staining (24-72h), No-wash staining, Real-time imaging

Cell permeability

Membrane permeant

Colors

Green, Far-red

Fixation options

Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining, Fix before staining (methanol), Fix before staining (formaldehyde)

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Technology

Dye
Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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50 uL
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Product Description

NucSpot® Live Cell Nuclear Stains are cell membrane-permeant DNA dyes that specifically stain nuclei in live or fixed cells. They have excellent specificity for DNA without the need for a wash step, with low toxicity for live cell imaging.

Features

  • No-wash, nuclear-specific DNA dyes
  • Low-toxicity for real-time live cell imaging
  • Fix before or after labeling
  • NucSpot® Live 650 compatible with SIM, STED, or STORM
  • Choose green or far-red fluorescence

Kit Components

  • NucSpot® Live Dye, 1000X in DMSO
  • Verapamil, 100 mM in DMSO

Spectral Properties

  • NucSpot® Live 488 Ex/Em: 500/515 nm
  • NucSpot® Live 650 Ex/Em: 650/675 nm

NucSpot® Live 488 stains nuclei with green fluorescence for the FITC channel, while NucSpot® Live 650 has far-red fluorescence for the Cy®5 channel. NucSpot® Live 650 dye also is compatible with super-resolution imaging by SIM or STED, and has been validated in STORM (Ref. 4). The dyes are supplied as 1000X stock solutions in DMSO, and are supplied with a vial of the efflux pump inhibitor verapamil for optional use, which may increase probe retention and live cell staining in some cell types. Note: NucSpot® Live 488 and NucSpot® Live 650 have dim blue fluorescence in the DAPI channel, and may not be suitable for multicolor imaging with blue probes.

NucSpot® Live Cell Nuclear Stains can be used to stain live gram-positive bacteria, but do not stain live gram-negative bacteria or yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms.

Also see our wide selection of other Membrane and Organelle Stains, or download the Cellular Stains Brochure.

Cy Dye is a registered trademark of GE Healthcare.

References

1. BBRC (2018) 507, 496e502. https://doi.org/10.1016/j.bbrc.2018.11.071 (40081)
2. Translational Oncol (2018), 11(3), 700-714. https://doi.org/10.1016/j.tranon.2018.03.003 (40081)
3. bioRxiv (2019), https://doi.org/10.1101/672147 (40081)
4. bioRxiv (2019), https://www.biorxiv.org/content/10.1101/559484v2 (40082)
5. Clin Cancer Res (2019) 25(14), 4332-4342. doi:10.1158/1078-0432.CCR-18-3312 (40081)
6. Int J Mol Sci (2018) 19, 1870. doi:10.3390/ijms19071870 (40081)
7. Reprod Sci (2020) 27(3), 779-786. doi: 10.1007/s43032-019-00102-8 (40081)

 

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