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NucSpot® Live Cell Nuclear Stains

NucSpot® Live Cell Nuclear Stains are cell-membrane permeable DNA dyes that specifically stains nuclei in live or fixed cells. They have excellent specificity for DNA without the need for a wash step, and they have low toxicity for live cell imaging.

Product Attributes

Dye

NucSpot® Live 488, NucSpot® Live 650

Cellular localization

Nucleus

For live or fixed cells

For fixed cells, For live/intact cells

Assay type/options

Long term staining (24-72h), No-wash staining, Real-time imaging

Cell permeability

Membrane permeant

Colors

Green, Far-red

Fixation options

Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining, Fix before staining (methanol), Fix before staining (formaldehyde)

Dye
Size
Catalog #
price
Qty
Trial size (10 uL)
50 uL
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Product Description

NucSpot® Live Cell Nuclear Stains are cell-membrane permeable DNA dyes that specifically stain nuclei in live or fixed cells. They have excellent specificity for DNA without the need for a wash step, and they have low toxicity for live cell imaging. The dyes are supplied as 1000X stock solutions in DMSO, and are supplied with a vial of verapamil, which may increase probe retention and staining in some cell types.

NucSpot® Live 488 stains nuclei with green fluorescence (Ex/Em 500/515 nm). NucSpot® Live 650 stains nuclei with far-red fluorescence (Ex/Em 650/675 nm) for detection in the Cy®5 channel. NucSpot® Live 650 dye also is compatible with super-resolution imaging by SIM or STED, and has been validated in STORM (3, 4). Note: NucSpot® Live 488 and NucSpot® Live 650 have dim blue fluorescence in the DAPI channel, and may not be suitable for multicolor imaging with blue probes.

NucSpot® Live Cell Nuclear Stains can be used to stain live gram-positive bacteria, but do not stain live gram-negative bacteria or yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms.

Also see our wide selection of other Membrane and Organelle Stains, or download the Cellular Stains Brochure.

Cy5 is a registered trademark of GE Healthcare.

References

40081:
1. Translational Oncology (2018), 11(3), 700-714. https://doi.org/10.1016/j.tranon.2018.03.003
2. Clinical Cancer Research (2019), doi:10.1158/1078-0432.CCR-18-3312
3. bioRxiv (2019), https://doi.org/10.1101/672147
4. bioRxiv (2019), https://www.biorxiv.org/content/10.1101/559484v2

40082:
1. bioRxiv (2019), https://doi.org/10.1101/559484

 

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