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NucSpot® Live Cell Nuclear Stains

No-wash, fluorescent DNA nuclear stains dyes with low toxicity for long-term live cell imaging or fixed cell staining. Choose green or far-red fluorescence.

Product Attributes


NucSpot® Live 488, NucSpot® Live 650

Probe cellular localization


For live or fixed cells

For fixed cells, For live/intact cells

Fixation options

Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining, Fix before staining (methanol), Fix before staining (formaldehyde)

Assay type/options

Long term staining (24-72h), No-wash staining, Real-time imaging

Cell permeability

Membrane permeant


Green, Far-red


NucSpot® Live 488: 500/515 nm, NucSpot® Live 650: 650/675 nm

Storage Conditions

Store at 2 to 8 °C, Protect from light

Learn more about


Catalog #
Trial size
1 kit
Clear selection
(Please select quantity above )

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Product Description

NucSpot® Live Cell Nuclear Stains are cell membrane-permeant DNA dyes that specifically stain nuclei in live or fixed cells. They have excellent specificity for DNA without the need for a wash step, with low toxicity for live cell imaging.


  • No-wash, nuclear-specific DNA dyes
  • Low-toxicity for real-time live cell imaging
  • Fix before or after labeling
  • NucSpot® Live 650 compatible with SIM, STED, or STORM
  • Choose green or far-red fluorescence

Kit Components

  • NucSpot® Live Dye, 1000X in DMSO
  • Verapamil, 100 mM in DMSO

Spectral Properties

  • NucSpot® Live 488 Ex/Em: 503/518 nm
  • NucSpot® Live 650 Ex/Em: 655/681 nm

NucSpot® Live 488 stains nuclei with green fluorescence for the FITC channel, while NucSpot® Live 650 has far-red fluorescence for the Cy®5 channel. NucSpot® Live 650 dye also is compatible with super-resolution imaging by SIM or STED, and has been validated in STORM (Ref. 4). The dyes are supplied as 1000X stock solutions in DMSO, and are supplied with a vial of the efflux pump inhibitor verapamil for optional use, which may increase probe retention and live cell staining in some cell types. Note: NucSpot® Live 488 and NucSpot® Live 650 have dim blue fluorescence in the DAPI channel, and may not be suitable for multicolor imaging with blue probes.

NucSpot® Live Cell Nuclear Stains can be used to stain live gram-positive bacteria, but do not stain live gram-negative bacteria or yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms.

Biotium also offers NucSpot® Nuclear Stains for fixed cells in a wide selection of colors from green to near-IR. Also see our wide selection of other Membrane and Organelle Stains, or download the Cellular Stains Brochure.

Cy Dye is a registered trademark of Cytiva.


  1. BBRC (2018) 507, 496e502. (40081)
  2. Translational Oncol (2018), 11(3), 700-714. (40081, EVOS FL Imager)
  3. bioRxiv (2019), (40081)
  4. bioRxiv (2019), (40082, STORM)
  5. Clin Cancer Res (2019) 25(14), 4332-4342. doi:10.1158/1078-0432.CCR-18-3312 (40081, in mounting medium)
  6. Int J Mol Sci (2018) 19, 1870. doi:10.3390/ijms19071870 (40081)
  7. Reprod Sci (2020) 27(3), 779-786. doi: 10.1007/s43032-019-00102-8 (40081)
  8. FEBS Open Bio (2020). DOI: 10.1002/2211-5463.13059 (40082)
  9. J Cell Phys (2021), jcp.30336. DOI: 10.1002/jcp.30336 (40081)
  10. Pharmaceutics 2021, 13, 10. DOI: 10.3390/pharmaceutics13010010 (40082)
  11. J Cell Physiol (2021), 1–12. DOI: 10.1002/jcp.30336 (40081, 3D cell culture)
  12. Antimicrob Agents Chemother (2021). DOI: 10.1128/AAC.00356-21 (40082, STED microscopy)

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