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PCR & DNA Amplification
qPCR Master Mixes
Fast Probe Master Mix

Fast Probe Master Mix

A qPCR master mix containing Cheetah™ Taq hotstart DNA polymerase, suitable for probe-based real-time PCR applications.

Fast Probe Master Mix

Product Attributes

Detection method/readout

PCR/qPCR
Reference dye

No ROX, With ROX
Storage Conditions

Store at -10 to -35 °C

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Technology

PCR & DNA Amplification
CoA

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FAQs

EvaGreen® Dye and Master Mixes
What is the difference between EvaGreen® and SYBR® Green?

EvaGreen® dye is spectrally similar to FAM or SYBR® Green I, which means no change in optical settings is required for using an EvaGreen®-based master mix. However, there are several differences between the dyes.

Lower background fluorescence: EvaGreen® dye has less background than SYBR® Green I due to its novel “release-on-demand” DNA-binding mechanism.

Brighter and non-inhibitory:  EvaGreen® is less inhibitory in the PCR reaction than SYBR® Green, permitting the use of saturating dye concentration for maximal signal and better high-resolution DNA melt analysis.

Safer and more environmentally friendly: EvaGreen® dye is the first and only PCR dye to date designed to be environmentally safe.

Dye stability: EvaGreen® dye is very stable both during storage and under PCR conditions. SYBR® Green I, on the other hand, is known to degrade following multiple freeze-thaw cycles and under PCR conditions. Moreover, decomposed SYBR® Green I is reported to be even more inhibitory to PCR.

Digital PCR: EvaGreen® dye is currently the only qPCR dye to be used in droplet digital PCR (ddPCR).


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Which EvaGreen® product, 20X in water or 2000X in DMSO, is best for qPCR?

For most qPCR applications, we recommend using EvaGreen® Dye, 20X  in water (31000), or one of our 2X EvaGreen® qPCR Master Mixes. EvaGreen® Dye, 2000X  in DMSO (31019) is offered for specialized applications requiring a concentrated dye solution.


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Can EvaGreen® Dye, 2000X in DMSO be diluted in water?

If necessary, EvaGreen® Dye, 2000X  in DMSO can be diluted in water or master mix for qPCR for a final concentration of 1X in the PCR reaction.


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Product Description

Fast Probe Master Mix is designed for fluorescent probe-based PCR applications. It is compatible with probes such as a TaqMan®, BHQ®, and Molecular Beacon. However for most PCR applications we recommend the Forget-Me-Not™ Universal Probe Master Mix, for superior activity.

  • 2X probe-based qPCR master mix for fast cycling protocols
  • Suitable for all fluorescent probe types, including TaqMan®, BHQ®, and Molecular Beacon
  • Contains Cheetah™ Taq hotstart DNA polymerase

Fast Probe Master Mix is available with or without ROX reference dye, for use with high ROX, low ROX, or no ROX instruments.

Cheetah Taq is covered under US and international patents. TaqMan is a registered trademark of Roche Diagnostics.
 

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