Flubida-2
Flubida-2 has been used to detect pH at a specific site in a cell such as cell organelles by directing the probe to where avidin fusion proteins are located. The probe is a conjugate of biotin and fluorescein diacetate, which is nonfluorescent until the probe has entered the cells and hydrolyzed by endoesterases.
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Product Description
Flubida-2 has been used to detect pH at a specific site in a cell such as cell organelles by directing the probe to where avidin fusion proteins are located. The probe is a conjugate of biotin and fluorescein diacetate, which is nonfluorescent until the probe has entered the cells and hydrolyzed by endoesterases. Flubida-2 is membrane-permeable and thus can be delivered into cells via simple incubation with the probe in a buffer.
- λEx/λEm: 492/517 nm (pH 9) (after hydrolysis)
- Off-white solid soluble in DMSO
- Store at 4°C
- C43H48N4O9S
- MW: 812.93
References
1. Chemistry and Biology 7, 197 (2000).