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Flubida-2

Flubida-2 has been used to detect pH at a specific site in a cell such as cell organelles by directing the probe to where avidin fusion proteins are located. The probe is a conjugate of biotin and fluorescein diacetate, which is nonfluorescent until the probe has entered the cells and hydrolyzed by endoesterases.

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Product Description

Flubida-2 has been used to detect pH at a specific site in a cell such as cell organelles by directing the probe to where avidin fusion proteins are located. The probe is a conjugate of biotin and fluorescein diacetate, which is nonfluorescent until the probe has entered the cells and hydrolyzed by endoesterases. Flubida-2 is membrane-permeable and thus can be delivered into cells via simple incubation with the probe in a buffer.

  • λExEm: 492/517 nm (pH 9) (after hydrolysis)
  • Off-white solid soluble in DMSO
  • Store at 4°C
  • C43H48N4O9S
  • MW: 812.93

References

1. Chemistry and Biology 7, 197 (2000).

 

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