4-Bromo A-23187, Free Acid
Similar to A-23187, 4-Bromo A-23187 is used for in situ calibrations of fluorescent Ca2+ indicators to equilibrate intracellular and extracellular calcium concentrations and to allow Mn2+ to enter cells to quench indicator fluorescence.
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EDC (or EDAC) (1-(3-Dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride)) has recently been found to be very useful for fixing in situ chelators, including the fluorescent ion indicators for histological studies following physiological experiments. EDC is also a widely used reagent to activate carboxy groups for amine coupling.
Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.
BAPTA, AM Ester
BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium.
Pluronic® F-127 20% Solution in DMSO
Ready-to-use solution of Pluronic® F-127, a nonionic detergent useful for solubilizing hydrophobic dye molecules such as AM esters of calcium indicator dyes to facilitate cell loading.