ExoBrite™ Streptavidin Magnetic Beads

Streptavidin-conjugated magnetic beads validated for isolation of extracellular vesicles (EVs) and exosomes when combined with a biotinylated antibody.

Size

Catalog #

price

Qty

5 mL

Clear selection

Add to Cart

This product is available by special order only.

Please fill in the inquiry form and we will contact you shortly.

Don't see what you're looking for? Contact us for more conjugation options.

Request Quote or Bulk Order

ExoBrite™ Streptavidin Magnetic Beads - Image 2

ExoBrite™ Streptavidin Magnetic Beads were used to isolate and detect EVs from tissue culture cell supernatant. The beads were first incubated with a biotinylated CD9 antibody, and then with concentrated MCF-7 cell supernatant. The EVs bound to the beads were stained with ExoBrite™ 560/585 CTB EV Stain and detected by flow cytometry in the PE channel (orange dots). Beads without antibody were also incubated with cell supernatant and stained, and no staining was seen (gray dots).

ExoBrite™ Streptavidin Magnetic Beads - Image 3

ExoBrite™ Streptavidin Magnetic Beads were used to isolate and detect EVs from tissue culture cell supernatant. The beads were first incubated with a biotinylated CD63 antibody, and then with concentrated MCF-7 cell supernatant. The EVs bound to the beads were stained with ExoBrite™ 560/585 CTB EV Stain and detected by flow cytometry in the PE channel (orange dots). Beads without antibody were also incubated with cell supernatant and stained, and no staining was seen (gray dots).

ExoBrite™ Streptavidin Magnetic Beads - Image 4

ExoBrite™ Streptavidin Magnetic Beads were used to isolate and detect EVs from tissue culture cell supernatant. The beads were first incubated with a biotinylated CD81 antibody, and then with concentrated MCF-7 cell supernatant. The EVs bound to the beads were stained with ExoBrite™ 560/585 CD9 Flow Antibody and detected by flow cytometry in the PE channel (orange dots). Beads without antibody were also incubated with cell supernatant and stained, and no staining was seen (gray dots).

ExoBrite™ Streptavidin Magnetic Beads - Image 5

ExoBrite™ Streptavidin Magnetic Beads were used to isolate and detect EVs from tissue culture cell supernatant. The beads were first incubated with a biotinylated CD81 antibody, and then with concentrated MCF-7 cell supernatant. The EVs bound to the beads were stained with ExoBrite™ 560/585 CTB EV Stain and detected by flow cytometry in the PE channel (orange dots). Beads without antibody were also incubated with cell supernatant and stained, and no staining was seen (gray dots).

View Spectra Viewer

Antibody Finder

Product Description

ExoBrite™ Streptavidin Magnetic Beads are streptavidin-coated and magnetic polystyrene beads (4.5 um diameter) that can be combined with a biotinylated antibody for capture of extracellular vesicles (EVs) and exosomes. The beads may be used to isolate EVs from cell culture medium or other biological fluids without an overnight precipitation step.

Features

Combine with a biotinylated antibody (not included) for isolation of EVs
Isolate EVs from biofluids without an overnight precipitation step
Lower background and higher sensitivity than competitors
Convenient magnetic-based separation
Detect downstream with microscopy, flow cytometry, or western blot

Antibodies against membrane surface markers that are enriched in EVs, such as tetraspanins (CD9, CD63, or CD81), are commonly used for this method of EV isolation. The bead-bound EVs may then be stained with antibody conjugates or other probes for detection using flow cytometry or fluorescence microscopy. The bead-bound EVs may also be lysed for analysis of protein content by western blot, or nucleic acid content by sequencing.

Explore validated EV detection solutions with ExoBrite™ EV stains and antibodies

Biotium offers several EV staining kits that are optimized for bright, sensitive staining of EVs and that can also be combined with antibody staining. This includes ExoBrite™ CTB EV Stains (cholera toxin subunit B), ExoBrite™ Annexin V EV Stains, and ExoBrite™ WGA EV Stains (wheat germ agglutinin). Biotium also offers a selection of fluorescent ExoBrite™ Flow Antibodies against CD9, CD63, and CD81 for multiparameter analysis of free or bead-bound EVs by flow cytometry. ExoBrite™ Western Antibody Conjugates are also available for near-infrared fluorescent western blotting of EV markers and purity controls.

Documents, Protocols, SDS and COA

Protocols

PI-28000

SDS

SDS 28000

Datasheet

Datasheet Download

COA

Request the Certificate of Analysis

FAQs

Exosome & EV Staining

Can other membrane stains besides ExoBrite™ True EV Membrane Stains be used to stain extracellular vesicles (EVs)?

While early studies of EVs attempted to use first-generation membrane dyes like DiI or PKH to stain EVs, more recently this class of dyes has been found to be largely unsuitable for EV staining due to their high degree of aggregation. Dye aggregation not only generates nonspecific particles that are indistinguishable from EVs in flow cytometry, but also results in poor EV labeling efficiency. Biotium developed the ExoBrite™ True EV Membrane Stains in response to our customers difficulties with using traditional membrane dyes to stain EVs. See our Literature Digest for more information.

Which of Biotium’s antibodies can be used to stain EVs?

We strongly recommend our ExoBrite™ Flow Antibody Conjugates for staining both purified or bead-bound EVs. The antibodies are validated and optimized to offer bright signal and low background. They are available against human or mouse CD9, CD63, and CD81 tetraspanin proteins.

Can extracellular vesicles (EVs) be stained with ExoBrite™ True EV Membrane Stains and antibodies concurrently?

Yes, EVs can be stained simultaneously with an ExoBrite™ True EV Membrane Stain and a fluorescent antibody.

With purified EVs, we have seen good results when EVs were stained in 500 mL of 1X ExoBrite™ plus 1 ug/mL fluorescent antibody. Please view our Product Information Sheet for detailed protocols.

View more FAQs