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Tyramides and Tyramide Signal Amplification Kits
TyraMax™ Amplification Dyes and Kits

TyraMax™ Amplification Dyes and Kits

Biotium’s next-generation tyramide dyes with the widest selection of bright, photostable, and chemically stable dyes for spatial biology.

TyraMax™ Amplification Dyes and Kits
TyraMax™ Amplification Dyes and Kits - Image 2
TyraMax™ Amplification Dyes and Kits - Image 3
TyraMax™ Amplification Dyes and Kits - Image 4
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TyraMax™ Amplification Dyes and Kits - Image 8
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Product Attributes

Functional group

Tyramide
Chemical reactivity (reacts with)

Tyrosine residues
Assay type/options

Tissue staining
Detection method/readout

Fluorescence microscopy
Storage Conditions

Store at 2 to 8 °C, Protect from light

Learn more about

Technology

Considerations for Immunofluorescence Staining
Tyramide Signal Amplification

Tech Tips

Tech Tip: Multi-Color Fluorescence Imaging Using Biotium’s Tyramide Amplification Kits
Tech Tip: Combined Direct and Indirect IF Using Primary Antibodies from the Same Host
Troubleshooting Tips for Fluorescence Staining

The Full Spectrum Blog

Blog: Seeing More and Brighter Colors with Tyramide Signal Amplification
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FAQs

Tyramide Signal Amplification (CF® Dye & Other Tyramides)
Do you offer alternatives to Akoya Opal™ tyramides?

Biotium developed TyraMax™ Amplification Dyes as high-performance TSA dyes, offering brighter, more photostable signals than Aluora® and Opal® reagents. They also remain stable in amplification buffer for up to 24 hours, facilitating automated staining workflows. TyraMax™ Dyes are offered as standalone dye solutions, as 3-color or 5-color dye sets plus DAPI counterstain, and in a sampler for custom panel optimization. See below for alternatives to specific Aluora® and Opal® reagents. Learn more about our reagents for tyramide signal amplification (TSA).

TyraMax™ Amplification Dyes

DyeAbs/Em (nm)Laser LineDetection channelDye FeaturesSizeCatalog No.
TyraMax™ 410/450408/452405 nmDAPI/ Alexa Fluor® 405Unique tyramide color, spectrally similar to Alexa Fluor® 40520 uL96134-20UL
100 uL96134-100UL
TyraMax™ 430/500421/497405 nmFITCBrighter than Aluora® 430 and Opal® 48020 uL96135-20UL
100 uL96135-100UL
TyraMax™ 400/550394/547405 nmFITCUnique tyramide color, spectrally similar to Pacific Orange®20 uL96136-20UL
100 uL96136-100UL
TyraMax™ 490/520497/518488 nmFITCBrighter than Opal® 520, replacement for Aluora® 48820 uL96137-20UL
100 uL96137-100UL
TyraMax™ 555/565594/569555 nm or 561 nmTRITCBrighter than Opal® 570, replacement for Aluora® 55520 uL96138-20UL
100 uL96138-100UL
TyraMax™ 560/580562/584555 nm or 561 nmTRITCAlternative to Aluora® 555, Opal® 570 with superior photostability20 uL96139-20UL
100 uL96139-100UL
TyraMax™ 630/650631/650633 nm or 640 nmCy®5Bright and photostable alternative to Aluora® 647, Opal® 65020 uL96140-20UL
100 uL96140-100UL
TyraMax™ 647/670650/670633 nm or 640 nmCy®5Brighter than Opal® 650, replacement for Aluora® 64720 uL96141-20UL
100 uL96141-100UL
TyraMax™ 660/680663/683633 nm or 640 nmAlexa Fluor® 680Unique tyramide color, spectrally similar to Alexa Fluor® 660. Brighter and more photostable Opal® 690 when excited at 640 nm20 uL96142-20UL
100 uL96142-100UL
TyraMax™ 680/700680/701685 nm (detectable with 640 nm excitation)Alexa Fluor® 680Brighter and more photostable than Opal® 690 when excited at 685 nm20 uL96143-20UL
100 uL96143-100UL
TyraMax™ 710/740711/736685 nmAlexa Fluor® 700Brighter and more photostable than Aluora® 70020 uL96144-20UL
100 uL96144-100UL
TyraMax™ 740/770742/768730 nmAlexa Fluor® 750Single-step detection, unlike Opal® 780. Stable in amplification buffer for up to 24 hours, unlike Aluora® 75020 uL96145-20UL
100 uL96145-100UL

TyraMax™ 3-Plex Amplification Dye Set with DAPI (Cat. No. 33029)

Component #Component NameSize
96137-100ULTyraMax™ 490/520 Amplification Dye, 100X, 100 uLFor 100 samples using 100 uL staining volume
96139-100ULTyraMax™ 560/580 Amplification Dye, 100X, 100 uL
96140-100ULTyraMax™ 630/650 Amplification Dye, 100X, 100 uL
99897-50ULDAPI, 1000X, 50 uL

TyraMax™ 5-Plex Amplification Dye Set with DAPI (Cat. No. 33030)

Component #Component NameSize
96137-100ULTyraMax™ 490/520 Amplification Dye, 100X, 100 uLFor 100 samples using 100 uL staining volume
96139-100ULTyraMax™ 560/580 Amplification Dye, 100X, 100 uL
96140-100ULTyraMax™ 630/650 Amplification Dye, 100X, 100 uL
96143-100ULTyraMax™ 680/700 Amplification Dye, 100X, 100 uL
96145-100ULTyraMax™ 740/770 Amplification Dye, 100X, 100 uL
99897-50ULDAPI, 1000X, 50 uL

TyraMax™ Amplification Dye Spectral Sampler (Cat. No. 33031)

Component #Component NameSize
96134-20ULTyraMax™ 410/450For 20 samples per vial using 100 uL staining volume
96135-20ULTyraMax™ 430/500
96136-20ULTyraMax™ 400/550
96137-20ULTyraMax™ 490/520
96138-20ULTyraMax™ 555/565
96139-20ULTyraMax™ 560/580
96140-20ULTyraMax™ 630/650
96141-20ULTyraMax™ 647/670
96142-20ULTyraMax™ 660/680
96143-20ULTyraMax™ 680/700
96144-20ULTyraMax™ 710/740
96145-20ULTyraMax™ 740/770

Aluora is a registered trademark of Thermo Fisher Scientific; Opal is a registered trademark of Akoya Biosciences.


View more FAQs
Can I incubate multiple primary antibodies combined with tyramide amplificaton?

Our Tyramide Amplification Kits have been demonstrated to be robust and versatile for multi-color fluorescence imaging, compatible with dye-labeled antibodies and various cell staining methods (see Figure 1).

To use a Tyramide Amplification Kit in addition to one or more dye-labeled antibodies, follow the kit protocol to fix and block samples; label with primary antibodies; then detect primary antibodies using secondary antibodies. Dye labeled secondary antibodies can be co-incubated with the HRP-conjugated secondary or HRP-streptavidin from the tyramide kit. After washing, perform the CF® Dye tyramide reaction according to the kit protocol. The tyramide reaction does not interfere with the binding of dye-labeled antibodies or other fluorescent staining reagents.

Performing multi-color detection with more than one dye tyramide on the same sample requires sequential tyramide staining reactions, followed by HRP inactivation or antibody stripping between each step. See our tech tip:

Multi-Color Fluorescence Imaging Using Biotium’s Tyramide Amplification Kits


View more FAQs
Can the CF® dye tyramides be used with in situ hybridization or RNAscope assays from ACD?

Yes, CF® dye tyramides can be used for in situ hybridization using Tyramide Signal Amplification1 (TSA). They are also compatible with the RNAscope assays  for RISH2.

1 doi: https://doi.org/10.1038/s41598-018-38171-5.

2 doi: http://dx.doi.org/10.1101/651083.


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Product Description

TyraMax™ Dyes are Biotium’s next generation of tyramide amplification dyes for spatial biology. The dyes have been designed to yield a brighter signal compared to our original CF® Dye tyramides, and have advantages in brightness, photostability, and working solution stability compared to other TSA dyes.

Features

  • Largest selection of tyramide colors available, with unique options from blue to near-IR
  • Stable in amplification buffer for at least 24 hours, including near-IR dyes
  • Advantages in brightness and/or photostability compared to other TSA dyes
  • Available as standalone dyes with trial sizes, and dye sets for multiplexing or panel development

Power Your Multiplex Panels with TyraMax™

Tyramide signal amplification (TSA), also known as Catalyzed Reporter Deposition (CARD), is a highly sensitive technique for detecting low-abundance targets in fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (FISH). The superior sensitivity of TSA and its compatibility with multiplexing by cyclic immunofluorescence (CycIF) has made it indispensable for spatial biology applications.

TyraMax™ Dyes were developed as high-performance TSA dyes, offering brighter, more photostable signals than Aluora® and Opal® reagents. They also remain stable in amplification buffer for up to 24 hours, facilitating automated staining workflows. For optimal results, Biotium recommends using Tyramide Amplification Buffer Plus (Cat. No. 22029) with TyraMax™ Amplification Dyes.

Widest Selection of Colors for Unrivaeled Panel Flexibility

PFA-fixed HeLa cells stained with WGA-HRP detected with TyraMax™ Amplification Dyes in Biotium’s Tyramide Amplification Buffer Plus. Imaged using a 40X oil objective on Evident FV4000 confocal system.

Superior Brightness Compared to Other TSA Reagents

TyraMax™ 430/500 has excellent brightness compared to other commerically available TSA dyes. PFA-fixed HeLa cells stained with WGA-HRP and detected with TyraMax™ 430/500 Amplification Dye, Opal® Polaris 480 Reagent (Ex/Em 450/500 nm), or Aluora® 430 Spatial dye in Biotium’s Tyramide Amplification Buffer Plus. Imaged using a 40X oil objective on Evident FV4000 confocal system with 405 nm excitation in the Alexa Fluor® 430 detection channel.

Signal Stability You Can Count On

TyraMax™ 630/650 has excellent brightness and photostability compared to other commercially available TSA dyes. PFA-fixed HeLa cells stained with WGA-HRP and detected with TyraMax™630/650 Amplification Dye, Opal® Polaris 650 Reagent, or Aluora® 647 Spatial dye. Imaged using a 40X oil objective on Evident FV4000 confocal system with 640 nm excitation in the Alexa Fluor® 647 detection channel. Photostability measurements were done on an Olympus IX71 epifluorescence microscope using a Cy®5 filter cube. Stained cells were imaged every 10 seconds for 120 seconds, and mean fluorescence was normalized to the first image taken.

 

TyraMax™ 740/770 is stable in working solution for at least 24 hours. OD-matched solutions of TyraMax™ 740/770 and Aluora® 750 dyes were prepared in Tyramide Amplification Buffer Plus with 0.0015% hydrogen peroxide. Absorbance was measured on an Agilent Cary 60 spectrophotometer, and then the solutions were left at room temperature, protected from light, and absorbance was measured at various time points up to 24 hours. TyraMax™ 740/770 was stable in the presence of hydrogen peroxide over 24 hours, while Aluora® 750 completely degrades overnight.

TyraMax™ Amplification Dyes are available with a wide choice of dye options for multiplexing by conventional or spectral imaging. TyraMax™ Dyes are offered as standalone dye solutions, as 3-color or 5-color dye sets plus DAPI counterstain, and in a sampler for custom panel optimization. See the tables below for a full list of TyraMax™ Dyes, kits, and recommended channels.

TyraMax™ Amplification Dyes

DyeAbs/Em (nm)Laser LineDetection channelDye FeaturesSizeCatalog No.
TyraMax™ 410/450408/452405 nmDAPI/ Alexa Fluor® 405Unique tyramide color, spectrally similar to Alexa Fluor® 40520 uL96134-20UL
100 uL96134-100UL
TyraMax™ 430/500421/497405 nmFITCBrighter than Aluora® 430 and Opal® 48020 uL96135-20UL
100 uL96135-100UL
TyraMax™ 400/550394/547405 nmFITCUnique tyramide color, spectrally similar to Pacific Orange®20 uL96136-20UL
100 uL96136-100UL
TyraMax™ 490/520497/518488 nmFITCBrighter than Opal® 520, replacement for Aluora® 48820 uL96137-20UL
100 uL96137-100UL
TyraMax™ 555/565594/569555 nm or 561 nmTRITCBrighter than Opal® 570, replacement for Aluora® 55520 uL96138-20UL
100 uL96138-100UL
TyraMax™ 560/580562/584555 nm or 561 nmTRITCAlternative to Aluora® 555, Opal® 570 with superior photostability20 uL96139-20UL
100 uL96139-100UL
TyraMax™ 630/650631/650633 nm or 640 nmCy®5Bright and photostable alternative to Aluora® 647, Opal® 65020 uL96140-20UL
100 uL96140-100UL
TyraMax™ 647/670650/670633 nm or 640 nmCy®5Brighter than Opal® 650, replacement for Aluora® 64720 uL96141-20UL
100 uL96141-100UL
TyraMax™ 660/680663/683633 nm or 640 nmAlexa Fluor® 680Unique tyramide color, spectrally similar to Alexa Fluor® 660. Brighter and more photostable Opal® 690 when excited at 640 nm20 uL96142-20UL
100 uL96142-100UL
TyraMax™ 680/700680/701685 nm (detectable with 640 nm excitation)Alexa Fluor® 680Brighter and more photostable than Opal® 690 when excited at 685 nm20 uL96143-20UL
100 uL96143-100UL
TyraMax™ 710/740711/736685 nmAlexa Fluor® 700Brighter and more photostable than Aluora® 70020 uL96144-20UL
100 uL96144-100UL
TyraMax™ 740/770742/768730 nmAlexa Fluor® 750Single-step detection, unlike Opal® 780. Stable in amplification buffer for up to 24 hours, unlike Aluora® 75020 uL96145-20UL
100 uL96145-100UL

TyraMax™ 3-Plex Amplification Dye Set with DAPI (Cat. No. 33029)

Component #Component NameSize
96137-100ULTyraMax™ 490/520 Amplification Dye, 100X, 100 uLFor 100 samples using 100 uL staining volume
96139-100ULTyraMax™ 560/580 Amplification Dye, 100X, 100 uL
96140-100ULTyraMax™ 630/650 Amplification Dye, 100X, 100 uL
99897-50ULDAPI, 1000X, 50 uL

TyraMax™ 5-Plex Amplification Dye Set with DAPI (Cat. No. 33030)

Component #Component NameSize
96137-100ULTyraMax™ 490/520 Amplification Dye, 100X, 100 uLFor 100 samples using 100 uL staining volume
96139-100ULTyraMax™ 560/580 Amplification Dye, 100X, 100 uL
96140-100ULTyraMax™ 630/650 Amplification Dye, 100X, 100 uL
96143-100ULTyraMax™ 680/700 Amplification Dye, 100X, 100 uL
96145-100ULTyraMax™ 740/770 Amplification Dye, 100X, 100 uL
99897-50ULDAPI, 1000X, 50 uL

TyraMax™ Amplification Dye Spectral Sampler (Cat. No. 33031)

Component #Component NameSize
96134-20ULTyraMax™ 410/450For 20 samples per vial using 100 uL staining volume
96135-20ULTyraMax™ 430/500
96136-20ULTyraMax™ 400/550
96137-20ULTyraMax™ 490/520
96138-20ULTyraMax™ 555/565
96139-20ULTyraMax™ 560/580
96140-20ULTyraMax™ 630/650
96141-20ULTyraMax™ 647/670
96142-20ULTyraMax™ 660/680
96143-20ULTyraMax™ 680/700
96144-20ULTyraMax™ 710/740
96145-20ULTyraMax™ 740/770
Aluora, and Alexa Fluor are registered trademarks of Thermo Fisher Scientific; Opal is a registered trademark of Akoya Biosciences; CY DYE is a registered trademark of Cytiva.
 

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