See below for our recommended alternatives to Akoya tyramides. Unless noted as a direct replacement, these tyramide dyes are spectrally similar alternatives. They have not been validated as direct replacements for use with Akoya kits or imaging systems.
CF® Dye Alternatives for Akoya Tyramides
Akoya Tyramide | Ex/Em (nm) | Biotium Tyramide | Ex/Em (nm) | Biotium Cat. No. | Notes | CF® Dye Features |
---|---|---|---|---|---|---|
TSA Coumarin | 402/443 | CF®405S | 404/431 | 92197 | Much brighter & more photostable alternative |
CF®405S Features |
Opal™ Polaris 480 | 450/500 | CF®430 | 426/498 | 96053 | Recommended for abundant targets |
CF®430 Features |
Opal™520 | 494/525 | CF®488A | 490/515 | 92171 | CF®488A Features | |
TSA Fluorescein | 494/517 | Fluorescein | 492/514 | 96018 | Direct replacement | See CF®488A for a brighter & much more photostable alternative |
TSA TMR TSA Cyanine 3 |
550/570 | Cyanine 555 | 555/565 | 96020 | Direct replacement for Cyanine 3 |
See CF®555 & CF®568 for brighter & more photostable alternatives. |
CF®555 | 555/565 | 96021 | Brighter & more photostable alternatives |
CF®555 Features | ||
CF®568 | 562/583 | 92173 | CF®568 Features | |||
Opal™570 | 550/570 | CF®550R | 551/577 | 96077 | CF®550R Features | |
CF®555 | 555/565 |
96021 | CF®555 Features | |||
TSA Plus Cyanine 3.5 | 581/596 | CF®583R | 586/609 | 96085 | Brighter & more photostable alternatives |
CF®583R Features |
CF®594 | 593/614 | 92174 | CF®594 Features | |||
Opal™620 | 588/616 | CF®583R | 586/609 | 96085 | CF®583R Features | |
TSA Cyanine 5 | 648/667 | CF®640R | 642/662 | 92175 | Brighter & more photostable alternative |
CF®640R Features |
CF®647 | 650/665 | 96022 | Brighter alternative | CF®647 Features | ||
CF®660R | 663/682 | 92195 | Less cross-talk with visible red dyes; bright & extremely photostable |
CF®660R Features | ||
TSA Plus Cyanine 5.5 Opal™690 |
673/692 676/694 |
CF®680R | 680/701 | 92196 | Bright & extremely photostable |
CF®680R Features |
Opal™ Polaris 780 | 750/770 | CF®754 | 745/786 | 96090 | Recommended for abundant targets |
Unique NIR dye for TSA |
TSA Biotin | N/A | Biotin-XX | N/A | 92176 | Direct replacement | |
TSA Plus DNP | N/A | DNP | N/A | 96019 | Direct replacement | |
TSA Plus DIG | N/A | N/A | N/A | N/A | N/A |
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