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Do you offer alternatives to Akoya Opal™ tyramides?

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See below for our recommended alternatives to Akoya tyramides. Unless noted as a direct replacement, these tyramide dyes are spectrally similar alternatives. They have not been validated as direct replacements for use with Akoya kits or imaging systems.

CF® Dye Alternatives for Akoya Tyramides

Akoya TyramideEx/Em (nm)Biotium TyramideEx/Em (nm)Biotium Cat. No.NotesCF® Dye Features
TSA Coumarin402/443CF®405S404/43192197Much brighter & more
photostable alternative
CF®405S Features
Opal™ Polaris 480450/500CF®430426/49896053Recommended
for abundant targets
CF®430 Features
Opal™520494/525CF®488A490/51592171CF®488A Features
TSA Fluorescein494/517Fluorescein492/51496018Direct replacementSee CF®488A for a brighter &
much more photostable alternative
TSA Cyanine 3
550/570Cyanine 555555/56596020Direct replacement
for Cyanine 3
See CF®555 & CF®568 for brighter
& more photostable alternatives.
CF®555555/56596021Brighter & more
photostable alternatives
CF®555 Features
CF®568562/58392173CF®568 Features
Opal™570550/570CF®550R551/57796077CF®550R Features
96021CF®555 Features
TSA Plus Cyanine 3.5581/596CF®583R586/60996085Brighter & more
photostable alternatives
CF®583R Features
CF®594593/61492174CF®594 Features
Opal™620588/616CF®583R586/60996085CF®583R Features
TSA Cyanine 5648/667CF®640R642/66292175Brighter & more
photostable alternative
CF®640R Features
CF®647650/665 96022Brighter alternativeCF®647 Features
CF®660R663/68292195Less cross-talk with visible red dyes;
bright & extremely photostable
CF®660R Features
TSA Plus Cyanine 5.5
CF®680R680/70192196Bright & extremely
CF®680R Features
Opal™ Polaris 780750/770CF®754745/78696090Recommended
for abundant targets
Unique NIR dye for TSA
TSA BiotinN/ABiotin-XXN/A92176Direct replacement
TSA Plus DNPN/ADNPN/A96019Direct replacement
Note: The CF® Dyes listed here are spectrally similar alternatives to Opal™ dyes, they have not been validated as direct replacements for use with Akoya's kits or imaging system. Opal is a trademark of Akoya Biosciences.


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