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NucSpot® Nuclear Stains

Cell membrane-impermeant, nuclear-specific counterstains. Suitable for fixed cells or staining dead cells in live cultures.

Product Attributes

Probe cellular localization

Nucleus

For live or fixed cells

For fixed cells

Assay type/options

DNA content/cell cycle profiling by flow cytometry, Live/dead discrimination, Long term staining (24-72h), No-wash staining, Real-time imaging, Tissue staining

Detection method/readout

Fluorescence microscopy, Flow cytometry

Cell permeability

Membrane impermeant

Apoptosis/viability marker

Dead cell stain

Fixation options

Permeabilize before staining, Fix before staining (methanol), Fix before staining (formaldehyde)

Colors

Green, Orange, Red, Far-red, Near-infrared

Storage Conditions

Store at 2 to 8 °C

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100 uL
200 uL
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Product Description

NucSpot® Nuclear Stains are cell membrane-impermeant, nuclear-specific counterstains available in a variety of colors from green to near-infrared (near-IR).

  • Nuclear-specific counterstains for fixed cells
  • Selectively stains dead cells in live culture
  • No wash required
  • Options for cell cycle profiling by flow cytometry in fixed/permeabilized cells
  • Color options for FITC, Cy®3, PE, Texas Red®, Cy®5, APC, Cy®5.5, and Cy®7 channels

NucSpot® Stains have minimal fluorescence until they bind to DNA and can be used for no-wash nuclear staining. Unlike other nucleic acid dyes such as propidium iodide (PI), TOTO®, TO-PRO®, and similar dyes that stain both the nucleus and cytoplasm, NucSpot® Nuclear Stains selectively stain the nucleus in fixed and permeabilized cells without the need for RNase treatment.

NucSpot® Nuclear Stains also can be used for selective staining of dead cells in unfixed cell cultures for analysis by flow cytometry or fluorescence imaging. Several of the dyes can be continuously incubated with cells for multi-day imaging. NucSpot® 470 and NucSpot® Far-Red can be used for DNA content analysis of cell cycle by flow cytometry in fixed and permeabilized cells without requiring RNase treatment, unlike propidium iodide.

NucSpot® Nuclear Stains

ProductEx/EmDetection ChannelSizeCatalog No.
NucSpot® 470461/547 nmFITC20 uL40083-T
200 uL40083
NucSpot® 555/570559/566 nmCy®3 or PE*20 uL41033-T
100 uL41033
NucSpot® 568/580572/583 nmCy®3 or PE*20 uL41036-T
100 uL41036
NucSpot® 594/615603/613 nmTexas Red® or PE-Texas Red®*20 uL41037-T
100 uL41037
NucSpot® 650/665653/671 nmCy®5 or APC*20 uL41034-T
100 uL41034
NucSpot® Far-Red597/667 nmPE-Cy®5 or APC†50 uL40085-T
500 uL40085
NucSpot® 680/700683/707 nmCy®5.5*20 uL41035-T
100 uL41035
NucSpot® 750/780757/780 nmCy®7 or APC-Cy®7*20 uL41038-T
100 uL41038
* May show crosstalk in lower wavelength detection channels. Perform single-stain controls before combining with other probes.
† NucSpot® Far-Red is designed for flow cytometry optical systems and is not recommended for fluorescence microscopy.

Find the Right Dye for Your Application

PFA-fixed, Triton® X-100 permeabilized HeLa cells stained with NucSpot® Nuclear Stains in PBS and imaged by confocal microscopy without a wash step. All stains were used at 1X concentration except for NucSpot® 750/800, which was used at 5X concentration in order to image using 640 nm excitation.

NucSpot® 470 has green fluorescence that can be imaged using standard settings for FITC. With an excitation maximum around 460 nm, it is an excellent match for instruments with blue LED excitation sources as well. It can be used for DNA content analysis of cell cycle by flow cytometry in fixed and permeabilized cells without requiring RNase treatment, unlike propidium iodide. NucSpot® 470 can also be used for nuclear staining of dead or permeabilized yeast. In gram-negative bacteria it selectively stains dead cells, while in gram-positive bacteria it may stain live cells.

NucSpot® 555/570 and NucSpot® 568/580 have orange and visible red fluorescence, respectively, and are nuclear-specific alternatives to PI and similar dyes.

NucSpot® 594/615 has deep red fluorescence for the Texas Red® channel.

NucSpot® 650/665 has far-red fluorescence with superior nuclear specificity compared to first-generation far-red nuclear stains such as RedDot™2 and Draq7™.

NucSpot® 680/700 and NucSpot® 750/780 are spectrally unique DNA stains for far-red and near-IR detection.

Biotium also offers NucSpot® Far-Red, a flow cytometry stain developed as an improved alternative to 7-AAD. It shows less bleed-through fluorescence in the PE-Texas Red® channel compared to 7-AAD and is ideal for selective detection of dead cells or DNA content analysis by flow cytometry without RNase treatment.

Learn more about our full selection of Cellular Stains for the nucleus and other organelles.

TOTO and TO-PRO are registered trademarks of Thermo Fisher Scientific; Draq is a trademark of BioStatus Ltd; CY is a registered trademark of Cytiva.
 

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