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Apoptosis & Viability Assays

Biotium offers a wide selection of assay kits for cell viability and cell death for microplate reader, flow cytometry or fluorescence microscopy. Products include:

The NucView® Caspase-3 Substrate comes in 3 colors, and measures caspase activity in real time, in live cells. MitoView™ 633 can measure the decrease in mitochondria membrane potential that occurs in early apoptosis. CF® Dye Annexin V conjugates come in many color options, and label PS on the membranes of late apoptotic cells. We also offer kits containing combinations of these probes. Live-or-Dye™ dead cell stains for flow or microscopy. Fluorescence- and absorbance-based microplate viability assays such as MTT, XTT, and resazurin. ATP-Glo™ Bioluminometric Cell Viability luciferase assay. ViaFluor® Cell Proliferation Kits.

Learn more about our wide selection of assays for cell proliferation, cell viability, and apoptosis.

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Propidium Iodide (PI)

From: $63 Sizes: 10 mL (1 mg/mL in Water), 2 mL (50 ug/mL in Buffer), 100 mgCatalog #:
40016, 40017, - 40048View allHide

A membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a mixed live-dead population and also used as a counterstain in multicolor fluorescent assays.

Steady-ATP™ HTS Viability Assay Kit

From: $90 Sizes: 120 assays, 1,000 assays, 10,000 assaysCatalog #:
30138-1, 30138-2, - 30138-3View allHide

A luciferase-based ATP cell viability assay offering high sensitivity, wide dynamic range, and long-lasting signal for high-throughput luminescence detection in microplates.

Annexin V Conjugates

From: $117 Sizes: 100 uL, 500 uLCatalog #:
29126, 29085, 29001, 29003, 29004, 29005, 29008, 29009, 29010, 29011, 29012, 29013, 29014, 29069, 29045-100uL, 29045-500uL, 29057-100uL, 29083, - 29057-500uLView allHide

Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.

NucSpot® 470 Nuclear Stain

From: $65 Sizes: 20 uL, 200 uLCatalog #:
40083-T, - 40083View allHide

Cell membrane-impermeant, nuclear-specific green counterstain. Suitable for fixed cells or staining dead cells in live cultures.

ViaFluor® SE Cell Proliferation Kits

From: $63 Sizes: Trial size (1 vial), Set of 10 vialsCatalog #:
30139, 30139-T, 30068-T, 30068, 30086-T, 30086, - 30050View allHide

Fixable cytoplasmic stains for monitoring cell division by flow cytometry. The dyes can also be used to track cell populations in co-culture.

Recombinant Annexin V (Lyophilized)

From: $172 Sizes: 100 ug, 1 mg, 5 mgCatalog #:
29089-100ug, 29088-100ug, 29088, 29089, - 29089-5mgView allHide

Recombinant human Annexin V expressed from E. coli as a single, non-glycosylated polypeptide chain. The protein is available with no tag or a C-terminal 6X His tag.

Oxazole Red (YO-PRO®-3), 1 mM in DMSO

From: $344 Sizes: 1 mLCatalog #: 40105

Oxazole Red, also known as YO-PRO®-3, is a far-red fluorescent, cell-impermeant nucleic acid stain that can be used to stain dead or fixed cells.

Oxazole Red Homodimer (YOYO®-3), 1 mM in DMSO

From: $515 Sizes: 200 uLCatalog #: 40106

Oxazole Red Homodimer, also known as YOYO®-3, is a far-red fluorescent, cell-impermeant nucleic acid stain that can be used to stain dead or fixed cells.

Oxazole Blue Homodimer (POPO™-1), 1 mM in DMSO

From: $309 Sizes: 100 uL (1 mM in DMSO)Catalog #: 40093

Oxazole Blue Homodimer, also known as POPO™-1, is a blue-fluorescent, cell-impermeant nucleic acid stain that can be used to stain dead or fixed cells.

Oxazole Yellow Homodimer (YOYO®-1), 1 mM in DMSO

From: $309 Sizes: 100 uL (1 mM in DMSO)Catalog #: 40090

Oxazole Yellow Homodimer, also known as YOYO®-1, is a green-fluorescent, cell-impermeant nucleic acid stain that can be used to stain dead or fixed cells.

Ac-DEVD-R110

From: $120 Sizes: 1 mgCatalog #: 10226

A fluorogenic substrate for caspase-3 that can be used to continuously measure the activity of caspase-3 in cell extracts, using a fluorometer or fluorescence microplate reader.

NucView® Caspase-3 Enzyme Substrates

From: $82 Sizes: 10 uL (1 mM in DMSO), 100 uL (1 mM in DMSO), 10 uL (1 mM in PBS), 100 uL (1 mM in PBS)Catalog #:
10405-T, 10405, 10407-T, 10407, 10402-T, 10402, 10403-T, 10403, 10406-T, 10406, 10408-T, - 10408View allHide

Fluorescent caspase-3/7 substrates for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or live cell imaging.

Oxazole Blue (PO-PRO™-1), 1 mM in DMSO

From: $379 Sizes: 1 mLCatalog #: 40091

Oxazole Blue, also known as PO-PRO™-1, is a blue-fluorescent, cell-impermeant nucleic acid stain that can be used to stain dead or fixed cells.

NucSpot® Far-Red, 1000X in DMSO

From: $91 Sizes: 50 uL, 0.5 mLCatalog #:
40085, - 40085-TView allHide

An improved alternative to 7-AAD showing less bleed-through fluorescence in the PE-Texas Red® channel. Ideal for selective detection of dead cells by flow cytometry.

Annexin V CF® Dye Conjugates, Azide-Free, Lyophilized

From: $76 Sizes: 5 ugCatalog #:
29085R-5ug, 29012R-5ug, 29009R-5ug, 29083R-5ug, 29005R-5ug, 29004R-5ug, 29010R-5ug, 29011R-5ug, 29008R-5ug, 29014R-5ug, 29003R-5ug, - 29069R-5ugView allHide

Preservative-free Annexin V conjugates are compatible with real-time staining of apoptotic cells for live cell imaging, fluorescence microscopy, or flow cytometry.

NucSpot® Nuclear Stains

From: $93 Sizes: 20 uL, 100 uL, 200 uLCatalog #:
41040, 41040-T, 41033, 41033-T, 41036, 41036-T, 41037, 41037-T, 41034, 41034-T, 41035, 41035-T, 41038, - 41038-TView allHide

Cell membrane-impermeant, nuclear-specific counterstains. Suitable for fixed cells or staining dead cells in live cultures.