Live-or-Dye™ Fixable Viability Staining Kits allow discrimination between live and dead cells by flow cytometry or microscopy. Live/dead stains are useful probes to include when analyzing cell surface protein expression by flow cytometry, because they allow intracellular fluorescence signal from dead cells with permeable plasma membranes to be excluded from analysis.
- Highly dead-cell specific
- Covalent labeling with reactive dyes
- Withstand fixation and permeabilization
- The most available colors
- Dye options designed for spectral flow
- Live-or-Dye Fixable Dead Cell Dye
- Anhydrous DMSO
Note: Number of assays is based on 1 mL reaction size (106 cells); actual number of assays may vary depending on staining volume
Biotium offers a wide selection of Live-or-Dye™ viability stains spanning the fluorescence spectrum, for maximal flexibility in multi-color analysis. Visit the Live-or-Dye™ Technology Page for more data and information.
How It Works
Live-or-Dye™ Fixable Viability Stains are cell membrane impermeable amine-reactive dyes. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. The dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining.
Live-or-Dye™ Fixable Viability Stains are dead cell specific in all cell types, including mammalian cells, bacteria and yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms.
Special Application Notes
Live-or-Dye™ 510/550 & 665/685 have been validated for use in spectral flow cytometry on the Cytek® Aurora.
Live-or-Dye™ 488/515, 568/583, 594/614, & 640/662 have been validated in both flow cytometry and fluorescence microscopy.
Live-or-Dye™ 787/808 offers the best performance for the 808 laser line, effectively freeing up the channels in the visible spectrum.
|32002, 32002-T||Live-or-Dye™ 350/448||355 nm||DAPI or Violet||347/448 nm||Flow Cytometry
|32003, 32003-T||Live-or-Dye™ 405/452||405 nm||Pacific Blue®||408/452 nm||Flow Cytometry
|32009, 32009-T||Live-or-Dye™ 405/545||405 nm||AmCyan||395/545 nm||Flow Cytometry
|32004, 32004-T||Live-or-Dye™ 488/515||488 nm||FITC||490/515 nm||Flow Cytometry,
|32012, 32012-T||Live-or-Dye™ 510/550||488 nm||Spectral scan||516/549 nm||Spectral Cytometry
|32005, 32005-T||Live-or-Dye™ 568/583||488 or 561 nm||PE||562/583 nm||Flow Cytometry,
|32006, 32006-T||Live-or-Dye™ 594/614||488 or 561 nm||PE-Texas Red®||593/614 nm||Flow Cytometry,
|32007, 32007-T||Live-or-Dye™ 640/662||633 or 640 nm||APC||642/662 nm||Flow Cytometry, Microscopy
|32013, 32013-T||Live-or-Dye™ 665/685||633 or 640 nm||Spectral scan||667/685 nm||Spectral Cytometry
|32008, 32008-T||Live-or-Dye™ 750/777||633 or 640 nm||APC-Cy®7||755/777 nm||Flow Cytometry
|32011, 32011-T||Live-or-Dye™ 787/808||785 or 808 nm||APC-Cy®7 or IR800||783/803 nm||Flow Cytometry
|32010, 32010-T||Live-or-Dye NucFix™ Red||488 or 532 nm||PE-Texas Red®||520/593 nm||Flow Cytometry,