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Live-or-Dye™ Fixable Viability Staining Kits

Live-or-Dye™ Fixable Viability Staining Kits are designed for discrimination between live and dead cells during flow cytometry. Live/dead stains are useful probes to include when analyzing cell surface protein expression by flow cytometry, because they allow intracellular fluorescence signal from dead cells with permeable plasma membranes to be excluded from analysis.

Product Attributes

Apoptosis/viability marker

Dead cell stain

For live or fixed cells

For live/intact cells, Covalent & fixable stains

Detection method/readout

Fluorescence microscopy, Flow cytometry

Assay type/options

Endpoint assay

Colors

Blue, Green, Orange, Red, Far-red, Near-infrared

Fixation options

Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining

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50 assays
200 assays
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Product Description

Live-or-Dye™ Fixable Viability Staining Kits are designed for discrimination between live and dead cells during flow cytometry. Live/dead stains are useful probes to include when analyzing cell surface protein expression by flow cytometry, because they allow intracellular fluorescence signal from dead cells with permeable plasma membranes to be excluded from analysis.

Live-or-Dye™ Fixable Viability Stains are cell membrane impermeable amine-reactive dyes. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. On live cells, the dyes react with surface proteins, but these are much less abundant than intracellular proteins, resulting in low staining levels compared to dead cells. The dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining.

Biotium offers a selection of eight different Live-or-Dye™ viability stains spanning the fluorescence spectrum, for maximal flexibility in multi-color analysis.

Catalog No.Viability DyeLaser lineEmission filterAbs/Em MaxApplications
32002, 32002-TLive-or-Dye™ 350/448355 nmDAPI or Violet347/448 nmFlow Cytometry
32003, 32003-TLive-or-Dye™ 405/452405 nmPacific Blue®408/452 nmFlow Cytometry
32009, 32009-TLive-or-Dye™ 405/545405 nmAmCyan395/545 nmFlow Cytometry,
Microscopy
32004, 32004-TLive-or-Dye™ 488/515488 nmFITC490/515 nmFlow Cytometry,
Microscopy
32005, 32005-TLive-or-Dye™ 568/583488 or 561 nmPE562/583 nmFlow Cytometry,
Microscopy
32006, 32006-TLive-or-Dye™ 594/614488 or 561 nmPE-Texas Red®593/614 nmFlow Cytometry,
Microscopy
32007, 32007-TLive-or-Dye™ 640/662633 or 640 nmAPC642/662 nmFlow Cytometry
32008, 32008-TLive-or-Dye™ 750/777633 or 640 nmAPC-Cy®7755/777 nmFlow Cytometry,
Microscopy
32010, 32010-TLive-or-Dye NucFix™ Red488 or 532 nmPE-Texas Red®520/610 nmFlow Cytometry,
Microscopy

References

Live-or-Dye™ 350 in bacteria: Poynter SJ et al. Dev Comp Immunol. 2017 Dec;77:95-105.

Live-or-Dye™ 640 in sperm: Majkowski M et al. PLoS ONE 2018. 13(12): e0208649.

Live-or-Dye™ 750 in PBMC flow: Ito S et al. Ann Surg Oncol. 2018. 25:3755–3763

 

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