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Live-or-Dye™ Fixable Viability Staining Kits

Kits to covalently label dead cells, allowing cells with permeable plasma membranes to be excluded from analysis in flow cytometry. Includes dye options for fluorescence microscopy.

Product Attributes

Apoptosis/viability marker

Dead cell stain

For live or fixed cells

Covalent & fixable stains, For live/intact cells

Detection method/readout

Fluorescence microscopy, Flow cytometry

Assay type/options

Endpoint assay

Fixation options

Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining

Colors

Blue, Green, Orange, Red, Far-red, Near-infrared

Storage Conditions

Store at -10 to -35 °C, Protect dye component from light, Desiccate

Excitation/Emission
Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

Live-or-Dye™ Fixable Viability Staining Kits allow discrimination between live and dead cells by flow cytometry or microscopy. Live/dead stains are useful probes to include when analyzing cell surface protein expression by flow cytometry, because they allow intracellular fluorescence signal from dead cells with permeable plasma membranes to be excluded from analysis.

Features

  • Highly dead-cell specific
  • Covalent labeling with reactive dyes
  • Withstand fixation and permeabilization
  • The most available colors
  • Dye options designed for spectral flow

Kit Components

  • Live-or-Dye Fixable Dead Cell Dye
  • Anhydrous DMSO
    Note: Number of assays is based on 1 mL reaction size (106 cells); actual number of assays may vary depending on staining volume

Biotium offers a wide selection of Live-or-Dye™ viability stains spanning the fluorescence spectrum, for maximal flexibility in multi-color analysis. Visit the Live-or-Dye™ Technology Page for more data and information.

How It Works

Live-or-Dye™ Fixable Viability Stains are cell membrane impermeable amine-reactive dyes. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. The dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining.

Live-or-Dye™ Fixable Viability Stains are dead cell specific in all cell types, including mammalian cells, bacteria and yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms.

Special Application Notes

Live-or-Dye™ 510/550 & 665/685 have been validated for use in spectral flow cytometry on the Cytek® Aurora.

Live-or-Dye™ 488/515, 568/583, 594/614, & 640/662 have been validated in both flow cytometry and fluorescence microscopy.

Live-or-Dye™ 787/808 offers the best performance for the 808 laser line, effectively freeing up the channels in the visible spectrum.

Catalog No.

Viability Dye

Laser line

Emission filter

Abs/Em Max

Applications

32002, 32002-TLive-or-Dye™ 350/448355 nmDAPI or Violet347/448 nmFlow Cytometry
32003, 32003-TLive-or-Dye™ 405/452405 nmPacific Blue®408/452 nmFlow Cytometry
32009, 32009-TLive-or-Dye™ 405/545405 nmAmCyan395/545 nmFlow Cytometry
32004, 32004-TLive-or-Dye™ 488/515488 nmFITC490/515 nmFlow Cytometry,
Microscopy
32012, 32012-TLive-or-Dye™ 510/550488 nmSpectral scan516/549 nmSpectral Cytometry
32005, 32005-TLive-or-Dye™ 568/583488 or 561 nmPE562/583 nmFlow Cytometry,
Microscopy
32006, 32006-TLive-or-Dye™ 594/614488 or 561 nmPE-Texas Red®593/614 nmFlow Cytometry,
Microscopy
32007, 32007-TLive-or-Dye™ 640/662633 or 640 nmAPC642/662 nmFlow Cytometry, Microscopy
32013, 32013-TLive-or-Dye™ 665/685633 or 640 nmSpectral scan667/685 nmSpectral Cytometry
32008, 32008-TLive-or-Dye™ 750/777633 or 640 nmAPC-Cy®7755/777 nmFlow Cytometry
32011, 32011-TLive-or-Dye™ 787/808785 or 808 nmAPC-Cy®7 or IR800783/803 nmFlow Cytometry
32010, 32010-TLive-or-Dye NucFix™ Red488 or 532 nmPE-Texas Red®520/593 nmFlow Cytometry,
Microscopy

References

1. Methods (2015) 84;pp44-52 doi:10.1016/j.ymeth.2015.04.025 Live-or-Dye™ 488
2. Dev Comp Immunol (2017) Dec;77:95-105. doi: 10.1016/j.dci.2017.07.021 Live-or-Dye™ 350
3. PLoS ONE (2018) 13(12): e0208649 doi.org/10.1371/journal.pone.0208649 Live-or-Dye™ 640
3. Ann Surg Oncol (2018) 25, 3755–3763 doi:10.1245/s10434-018-6739-4 Live-or-Dye™ 750
4. MBio (2018) Aug 21;9(4) doi:10.1128/mBio.01403-18
5. Cell Physiol Biochem (2018) 49:828–836 doi:10.1159/000493110 Live-or-Dye™ 350
6. FEBS J (2019) Nov 26. doi:10.1111/febs.15156 Live-or-Dye™ 488
7. JBC (2019) 294, 16918-16929 doi:10.1074/jbc.RA119.009141 Live-or-Dye™ 488

 

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