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Live-or-Dye™ Fixable Viability Staining Kits

Kits to covalently label dead cells, allowing cells with permeable plasma membranes to be excluded from analysis in flow cytometry. A wide variety of dye options for standard flow, spectral flow, and fluorescence microscopy.

Product Attributes

Apoptosis/viability marker

Dead cell stain

For live or fixed cells

Covalent & fixable stains, For live/intact cells

Detection method/readout

Fluorescence microscopy, Flow cytometry

Assay type/options

Endpoint assay

Fixation options

Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining


Blue, Green, Orange, Red, Far-red, Near-infrared

Storage Conditions

Store at -10 to -35 °C, Protect dye component from light, Desiccate

Catalog #
50 assays
200 assays
250 assays
Clear selection
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Product Description

Live-or-Dye™ Fixable Viability Staining Kits allow discrimination between live and dead cells by flow cytometry or microscopy. Live/dead stains are useful probes to include when analyzing cell surface protein expression by flow cytometry, because they allow intracellular fluorescence signal from dead cells with permeable plasma membranes to be excluded from analysis.


  • Highly dead-cell specific
  • Covalent labeling with reactive dyes
  • Withstand fixation and permeabilization
  • Wide selection of colors
  • Dye options designed for spectral flow
  • Sampler kits for convenience

Single Color Kit Components

  • Live-or-Dye™ fixable dead cell dye
  • Anhydrous DMSO

Sampler Kit Components

  • 5 different Live-or-Dye™ fixable dead cell dyes
  • Anhydrous DMSO

Biotium offers a wide selection of Live-or-Dye™ viability stains spanning the fluorescence spectrum, for maximal flexibility in multi-color analysis. Visit the Live-or-Dye™ Technology Page for more data and information.

How It Works

Live-or-Dye™ Fixable Viability Stains are cell membrane impermeant amine-reactive dyes. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. The dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining.

Biotium has a wide selection of Live-or-Dye™ viability stains spanning the fluorescence spectrum (see Table 1 below), for maximal flexibility in designing multi-color flow panels.

We also offer two sampler kits, each containing five different dye colors, for excitation from all of the popular flow cytometry laser lines (see Table 2 below). The Live-or-Dye™ Sampler Kit, Standard (32016) was designed for use on the most common flow cytometer laser and filter configurations, with dyes excitable by UV, Violet, Blue, Yellow-Green, and Red lasers. The Live-or-Dye™ Sampler Kit, Spectral (32017) was designed for use in spectral scanning flow cytometry. It contains dyes excitable by UV, Violet, Blue, and Red lasers, all of which have been validated on the Cytek® Aurora spectral cytometer, and chosen for their ability to fill the gaps in many spectral flow panels.

Live-or-Dye™ Fixable Viability Stains are dead cell specific in all cell types, including mammalian cells, bacteria and yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms.

Table 1: Single-Color Live-or-Dye™ Kits

Cat. No.

Viability Dye

Compatible lasers (nm)

Optimal detection channels


32018, 32018-TLive-or-Dye™ 330/410355, 375BUV395
32002, 32002-TLive-or-Dye™ 350/448355, 375DAPI
32014,32014-TLive-or-Dye™ 375/600355, 375, 405Spectral scan, BUV615, BV605Developed for spectral cytometry
32003, 32003-TLive-or-Dye™ 405/452405Pacific Blue®, BV421, BV450
32009, 32009-TLive-or-Dye™ 405/545405AmCyan, BV510
32004, 32004-TLive-or-Dye™ 488/515488FITCValidated for microscopy
32012, 32012-TLive-or-Dye™ 510/550488, 532Spectral scanDeveloped for spectral cytometry
32005, 32005-TLive-or-Dye™ 568/583488, 532, 561PE, PIValidated for microscopy
32006, 32006-TLive-or-Dye™ 594/614488, 532, 561PI, PE-CF®594, PE-Texas Red®Validated for microscopy
32015, 32015-TLive-or-Dye™ 615/740633-640Spectral scan, APC-Cy®7Developed for spectral cytometry
32007, 32007-TLive-or-Dye™ 640/662633-640APCValidated for microscopy
32013, 32013-TLive-or-Dye™ 665/685633-640Spectral scan, AF700Developed for spectral cytometry
32008, 32008-TLive-or-Dye™ 750/777633-640, 785APC-Cy®7The brightest dye for the APC-Cy®7 channel
32011, 32011-TLive-or-Dye™ 787/808785, 808APC-Cy®7, IR800

Table 2: Live-or-Dye™ Sampler Kits

Kit Name

Catalog No.

Included dyes: Ex/Em (Cat No.)


Live-or-Dye™ Fixable Viability Sampler Kit, Standard32016• 350/448 (32002A)
• 405/545 (32009A)
• 488/515 (32004A)
• 568/583 (32005A)
• 640/662 (32007A)
Designed for use on most standard flow cytometry laser and filter configurations
Live-or-Dye™ Fixable Viability Sampler Kit, Spectral32017• 350/448 (32002A)
• 375/600 (32014A)
• 510/550 (32012A)
• 615/740 (32015A)
• 665/685 (32013A)
Designed for use in spectral flow cytometry, to fill in gaps between common fluorophores

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