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Live-or-Dye™ Fixable Viability Staining Kits

Live-or-Dye™ Fixable Viability Staining Kits stain dead cells during flow cytometry to allow dead cells with permeable plasma membranes to be excluded from analysis.

Product Attributes

Excitation/Emission

350/448, 405/452, 405/545, 488/515, 510/550, 568/583, 594/614, 640/662, 665/685, 750/777

Apoptosis/viability marker

Dead cell stain

For live or fixed cells

Covalent & fixable stains, For live/intact cells

Detection method/readout

Fluorescence microscopy, Flow cytometry

Assay type/options

Endpoint assay

Fixation options

Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining

Colors

Blue, Green, Orange, Red, Far-red, Near-infrared

Excitation/Emission
Size
Catalog #
price
Qty
50 assays
200 assays
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Product Description

Live-or-Dye™ Fixable Viability Staining Kits are designed for discrimination between live and dead cells during flow cytometry. Live/dead stains are useful probes to include when analyzing cell surface protein expression by flow cytometry, because they allow intracellular fluorescence signal from dead cells with permeable plasma membranes to be excluded from analysis.

Live-or-Dye™ Fixable Viability Stains are cell membrane impermeable amine-reactive dyes. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. On live cells, the dyes react with surface proteins, but these are much less abundant than intracellular proteins, resulting in low staining levels compared to dead cells. The dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining.

Live-or-Dye™ Fixable Viability Stains are dead cell specific in all cell types, including mammalian cells, bacteria and yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms.

Biotium offers a selection of ten different Live-or-Dye™ viability stains spanning the fluorescence spectrum, for maximal flexibility in multi-color analysis.

Special application notes

Live-or-Dye™ 510/550 & 665/685 have been validated for use in spectral flow cytometry on the Cytek® Aurora.

Live-or-Dye™ 488/515, 568/583, 594/614, & 640/662 have been validated for use in fluorescence microscopy.

 

Live-or-Dye™ Fixable Viability Staining Kits

Catalog No.

Viability Dye

Laser line

Emission filter

Abs/Em Max

Applications

32002, 32002-TLive-or-Dye™ 350/448355 nmDAPI or Violet347/448 nmFlow Cytometry
32003, 32003-TLive-or-Dye™ 405/452405 nmPacific Blue®408/452 nmFlow Cytometry
32009, 32009-TLive-or-Dye™ 405/545405 nmAmCyan395/545 nmFlow Cytometry,
Microscopy
32004, 32004-TLive-or-Dye™ 488/515488 nmFITC490/515 nmFlow Cytometry,
Microscopy
32012, 32012-TLive-or-Dye™ 510/550488 nmSpectral scan516/549 nmSpectral Cytometry
32005, 32005-TLive-or-Dye™ 568/583488 or 561 nmPE562/583 nmFlow Cytometry,
Microscopy
32006, 32006-TLive-or-Dye™ 594/614488 or 561 nmPE-Texas Red®593/614 nmFlow Cytometry,
Microscopy
32007, 32007-TLive-or-Dye™ 640/662633 or 640 nmAPC642/662 nmFlow Cytometry
32013, 32013-TLive-or-Dye™ 665/685633 or 640 nmSpectral scan667/685 nmSpectral Cytometry
32008, 32008-TLive-or-Dye™ 750/777633 or 640 nmAPC-Cy®7755/777 nmFlow Cytometry,
Microscopy
32010, 32010-TLive-or-Dye NucFix™ Red488 or 532 nmPE-Texas Red®520/593 nmFlow Cytometry,
Microscopy

References

Live-or-Dye™ 350 in bacteria: Poynter SJ et al. Dev Comp Immunol. 2017 Dec;77:95-105.

Live-or-Dye™ 640 in sperm: Majkowski M et al. PLoS ONE 2018. 13(12): e0208649.

Live-or-Dye™ 750 in PBMC flow: Ito S et al. Ann Surg Oncol. 2018. 25:3755–3763

Live-or-Dye™ in Candida aurus microscopy: Johnson et al. MBio. 2018. 10.1128/mBio.01403-18

 

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