Live-or-Dye™ Fixable Viability Staining Kits allow discrimination between live and dead cells by flow cytometry or microscopy. Live/dead stains are useful probes to include when analyzing cell surface protein expression by flow cytometry, because they allow intracellular fluorescence signal from dead cells with permeable plasma membranes to be excluded from analysis.
- Highly dead-cell specific
- Covalent labeling with reactive dyes
- Withstand fixation and permeabilization
- The most available colors
- Dye options designed for spectral flow
- Sampler kits for convenience
Single Color Kit Components
- Live-or-Dye™ fixable dead cell dye
- Anhydrous DMSO
Sampler Kit Components
- 5 different Live-or-Dye™ fixable dead cell dyes
- Anhydrous DMSO
Biotium offers a wide selection of Live-or-Dye™ viability stains spanning the fluorescence spectrum, for maximal flexibility in multi-color analysis. Visit the Live-or-Dye™ Technology Page for more data and information.
How It Works
Live-or-Dye™ Fixable Viability Stains are cell membrane impermeant amine-reactive dyes. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. The dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining.
Biotium has the widest selection of Live-or-Dye™ viability stains spanning the fluorescence spectrum (see Table 1 below), for maximal flexibility in designing multi-color flow panels.
We also offer two sampler kits, each containing five different dye colors, for excitation from all of the popular flow cytometry laser lines (see Table 2 below). The Live-or-Dye™ Sampler Kit, Standard (32016) was designed for use on the most common flow cytometer laser and filter configurations, with dyes excitable by UV, Violet, Blue, Yellow-Green, and Red lasers. The Live-or-Dye™ Sampler Kit, Spectral (32017) was designed for use in spectral scanning flow cytometry. It contains dyes excitable by UV, Violet, Blue, and Red lasers, all of which have been validated on the Cytek® Aurora spectral cytometer, and chosen for their ability to fill the gaps in many spectral flow panels.
Live-or-Dye™ Fixable Viability Stains are dead cell specific in all cell types, including mammalian cells, bacteria and yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms.
Special Application Notes
Live-or-Dye™ 350/448, 375/600, 510/550, 615/740 & 665/685 have been validated for use in spectral flow cytometry on the Cytek® Aurora.
Live-or-Dye™ 488/515, 568/583, 594/614, & 640/662 have been validated in both flow cytometry and fluorescence microscopy.
Live-or-Dye™ 787/808 offers the best performance for the 808 laser line, effectively freeing up the channels in the visible spectrum.
Table 1: Single-Color Live-or-Dye™ Kits
Compatible lasers (nm)
Optimal detection channels
|32002, 32002-T||Live-or-Dye™ 350/448||355, 375||DAPI||
|32014,32014-T||Live-or-Dye™ 375/600||355, 375, 405||Spectral Scan, BUV615, BV605||Developed for spectral cytometry
|32003, 32003-T||Live-or-Dye™ 405/452||405 nm||Pacific Blue®, BV421, BV450||
|32009, 32009-T||Live-or-Dye™ 405/545||405 nm||AmCyan, BV510||
|32004, 32004-T||Live-or-Dye™ 488/515||488 nm||FITC||Validated for microscopy
|32012, 32012-T||Live-or-Dye™ 510/550||488, 532||Spectral scan||Developed for spectral cytometry
|32005, 32005-T||Live-or-Dye™ 568/583||488, 532, 561||PE, PI||Validated for microscopy
|32006, 32006-T||Live-or-Dye™ 594/614||488, 532, 561||PI, PE-CF®594, PE-Texas Red®||Validated for microscopy
|32015, 32015-T||Live-or-Dye™ 615/740||633-640||Spectral scan, APC-Cy®7||Developed for spectral cytometry
|32007, 32007-T||Live-or-Dye™ 640/662||633-640||APC||Validated for microscopy
|32013, 32013-T||Live-or-Dye™ 665/685||633-640||Spectral scan, AF700||Developed for spectral cytometry
|32008, 32008-T||Live-or-Dye™ 750/777||633-640, 785||APC-Cy®7||The brightest dye for the APC-Cy®7 channel
|32011, 32011-T||Live-or-Dye™ 787/808||785, 808||APC-Cy®7, IR800||
Table 2: Live-or-Dye™ Sampler Kits
Included dyes: Ex/Em (Cat No.)
|Live-or-Dye™ Fixable Viability Sampler Kit, Standard||32016||• 350/488 (32002A)|
• 405/545 (32009A)
• 488/515 (32004A)
• 568/583 (32005A)
• 640/662 (32007A)
|Designed for use on most standard flow cytometry laser and filter configurations
|Live-or-Dye™ Fixable Viability Sampler Kit, Spectral||32017||• 350/448 (32002A)|
• 375/600 (32014A)
• 510/550 (32012A)
• 615/740 (32015A)
• 665/685 (32013A)
|Designed for use in spectral flow cytometry, to fill in gaps between common fluorophores