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ViaFluor® SE Cell Proliferation Kits

Fixable cytoplasmic stains for monitoring cell division by flow cytometry. The dyes can also be used to track cell populations in co-culture.

Product Attributes

Dye Color

ViaFluor® CFSE, ViaFluor® 405, ViaFluor® 488

Apoptosis/viability marker

All cell stain

Cellular localization

Nucleus & cytoplasm

For live or fixed cells

Covalent & fixable stains, For live/intact cells

Detection method/readout

Fluorescence microscopy, Flow cytometry

Assay type/options

Cell division tracking, Extended staining (several days to weeks), Real-time imaging

Cell permeability

Membrane permeant

Colors

Blue, Green

Fixation options

Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining

Storage Conditions

Store at -10 to -35 °C, Protect from light, Desiccate

Dye Color
Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

ViaFluor®  SE Cell Proliferation Kits use amine-reactive dyes to covalently label cells throughout the cell cytoplasm and intracellular compartments for fixable fluorescent staining. Cell proliferation dyes are commonly used to monitor cell division by flow cytometry. The dyes also can be used to stably label cells to image cell morphology, or to track cell populations in mixed co-culture experiments.

Features

  • Non-toxic dyes covalently label cell cytoplasm for fixable staining
  • Track cell proliferation in vivo or in vitro by dye dilution using flow cytometry
  • Long term imaging of cell morphology or co-cultures by microscopy
  • Excellent performance & lower cost compared to leading competitors
  • ViaFluor® 405 & ViaFluor® 488 SE are much less toxic than CFSE

Kit Components

  • Lyophilized dye in single use vials
  • Anhydrous DMSO for dissolving dye

Spectral Properties (Ex/Em after hydrolysis)

  • ViaFluor® CFSE: 495/515 nm
  • ViaFluor® 488 SE: 493/532 nm
  • ViaFluor® 405 SE: 408/452 nm

ViaFluor® SE Dyes for Cell Proliferation Tracking

ViaFluor®  SE dyes are membrane permeable compounds that are non-fluorescent until they enters viable cells, where the compound is hydrolyzed by cytoplasmic esterase enzymes to releases the fluorescent amine-reactive dye. The dyes then covalently react with amine groups on intracellular proteins, forming fluorescent conjugates that are retained in the cell, while excess unreacted dye is washed away.  Immediately after staining, a single, bright fluorescent population will be detected by flow cytometry. With each cell division, daughter cells inherit roughly half of the fluorescent label, allowing the number of cell divisions that occur after labeling to be detected by the appearance of successively dimmer fluorescent peaks on a flow cytometry histogram compared to cells analyzed immediately after staining.  Thus, cell proliferation dyes can be used to track multiple cell divisions of cells grown in culture or injected in vivo after labeling with the ViaFluor® SE dye.

The number of assays that can be performed per kit depends on the dye concentration used (see the product protocol for more information). When used at 1 uM to label 106 cells in one mL, each dye vial can be used for 90-100 labelings.

Other Applications for ViaFluor® SE Dyes

ViaFluor® SE dyes also can be used for imaging cell morphology or identifying cells in co-culture by microscopy. Because the staining is non-toxic and well-retained, it can be used for imaging  live cells over time. See our Tech Tip: Using ViaFluor® SE Stains for Cell Tracing and Co-Culture.

All three ViaFluor® SE dyes can stain gram-positive bacteria, but not gram-negative bacteria. ViaFluor® CFSE stains the cytoplasm in yeast, but ViaFluor® 405 & ViaFluor® 488 stain the yeast cell periphery. See our Cellular Stains Table for more information on how our dyes stain various organisms.

Cell Division
Tracking Dyes

Catalog No.

Ex/Em (nm)

Flow detection
channel

Features

ViaFluor® 405 Cell Proliferation Kit30068408/452 Pacific Blue®• Track cell division by dye dilution using flow cytometry

• ViaFluor® 488 is a unique, improved green dye to replace CFSE

• ViaFluor® 405 replaces CellTrace™ Violet
ViaFluor® 488 Cell Proliferation Kit30086493/532FITC
ViaFluor® CFSE Cell Proliferation Kit30050495/519FITC
CellTrace is a trademark and Pacific Blue is a registered trademark of Thermo Fisher Scientific.

References

1. Mol Metab (2018) 16, 12e23. https://doi.org/10.1016/j.molmet.2018.07.004
2. Cell (2019) 177, 1903–1914. DOI: 10.1016/j.cell.2019.04.004
3. Sci Rep (2019) 9, 17470. https://doi.org/10.1038/s41598-019-53899-4
4. Sci Rep (2020) 10, 798. https://doi.org/10.1038/s41598-020-57602-w

 

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