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FAM SE, 5 and 6 isomers

5(6)-FAM SE (full name: 5-(and-6)-Carboxyfluorescein, succinimidyl ester mixed isomers) is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5.

Product Attributes

CAS number

117548-22-8

Excitation/Emission

495/519 nm

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Product Description

5(6)-FAM SE (full name: 5-(and-6)-Carboxyfluorescein, succinimidyl ester mixed isomers) is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5. The amide linkage from the coupling reaction is much more stable than the thiourea linkage formed from the coupling of an amine and an isothiocyanate.

  • λExEm = 495/519 nm
  • Yellow solid soluble in DMSO or DMF
  • Store at -20°C and protect from light
  • C25H15NO9
  • MW: 473.4
  • [117548-22-8]

 

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