Support & Resources

+1

FAM SE, 5 and 6 isomers

5(6)-FAM SE (full name: 5-(and-6)-Carboxyfluorescein, succinimidyl ester mixed isomers) is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5.

Product Attributes

CAS number

117548-22-8

Excitation/Emission

495/519 nm

Learn more about

Technology

Size
Catalog #
price
Qty
100 mg
Clear selection
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Protocols
SDS

Product Description

5(6)-FAM SE (full name: 5-(and-6)-Carboxyfluorescein, succinimidyl ester mixed isomers) is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5. The amide linkage from the coupling reaction is much more stable than the thiourea linkage formed from the coupling of an amine and an isothiocyanate.

  • λExEm = 495/519 nm
  • Yellow solid soluble in DMSO or DMF
  • Store at -20°C and protect from light
  • C25H15NO9
  • MW: 473.4
  • [117548-22-8]

 

You may also like…

    CF® Dye SE/TFP Ester

    CF® Dye SE/TFP esters are amine-reactive fluorescent dyes. They are commonly used to label primary amines of antibodies and other proteins, amine-modified oligonucleotides and other amine-containing molecules.

    DMSO, Anhydrous

    Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.

    Cyanine NS Dye Succinimidyl Ester

    Cyanine NS succinimidyl esters are non-sulfonated cyanine dyes that can be used to label proteins, oligonucleotides and nucleic acids.

    TAMRA SE, 5 and 6 isomers

    5(6)-TAMRA SE (full name: 5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester (mixed isomers)) is the amine reactive form of TAMRA, one of the most commonly used red fluorescent dyes. The succinimidyl ester reacts readily with primary or secondary amines under mild conditions.

    5-Aminoallyl-dUTP (AA-dUTP), 10 mM solution

    5-Aminoallyl-dUTP (AA-dUTP) can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling method.

    Annexin V Conjugates

    Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.