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CF® Dye SE/TFP Ester

CF® Dye SE/TFP esters are amine-reactive fluorescent dyes. They are commonly used to label primary amines of antibodies and other proteins, amine-modified oligonucleotides and other amine-containing molecules.

Product Attributes

Chemical reactivity (reacts with)

Amines

Functional group

Tetrafluorophenyl Ester (TFP Ester), Succinimidyl Ester (NHS Ester)

Storage Conditions

Store at -10 to -35 °C, Protect from light

Conjugation
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0.25 umol
1 umol
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Product Description

CF® Dye SE/TFP Esters are amine-reactive forms of Biotium’s bright and photostable CF® Dyes. The succinimidyl ester (SE) group, also known as N-hydroxysuccinimide (NHS) esters, on the dye molecule reacts with primary amines such as lysine residues of antibodies or proteins, amine-modified oligonucleotides and other amine-containing molecules to form a stable amide linkage. CF® Dye TFP (tetrafluorophenyl) esters are more stable alternatives to succinimidyl ester. CF® Dye TFP esters are available for CF®850 and CF®870, Biotium’s industry-leading near-infrared dyes with emission above 850 nm.

  • Amine-reactive fluorescent dyes with high labeling efficiency
  • Label antibodies, proteins, amine-modified oligonucleotides and other amine-containing molecules.
  • Offered with Biotium’s bright and photostable CF® Dyes which provide superior signal-to-noise
  • Available in 36 CF® Dye colors from blue to near-IR wavelengths
  • Near-IR CF®850 and CF®870 TFP esters offer emission above 850 nm

Due to Biotium’s unique manufacturing and packaging process, our amine-reactive CF® Dyes are provided with the highest purity and more stable ester dye forms than other vendors. As a result, our CF® Dye SE/TFP products retain more reactivity and provide a higher labeling efficiency for users.

Superior CF® Dyes

CF® Dyes are Biotium’s line of next-generation fluorescent dyes that have improved brightness, photostability, and water solubility compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes. For more information download the CF® Dye Brochure.

Also see our CF® Dye SE Protein Labeling Kits and CF® Dye Mix-n-Stain™Antibody Labeling Kits.

CF® Dye SE/TFP Ester

DyeEx/EmSizeCatalog No.Dye Features
CF®350 SE347/448 nm1 umol92109CF®350 Features
CF®405S SE404/431 nm1 umol92110CF®405S Features
CF®405M SE408/452 nm1 umol92111CF®405M Features
CF®405L SE395/545 nm1 umol92112CF®405L Features
CF®430 SE426/498 nm1 umol92117CF®430 Features
CF®440 SE440/515 nm1 umol92123CF®440 Features
CF®450 SE450/538 nm1 umol96011CF®450 Features
CF®488A SE490/515 nm1 umol92120CF®488A Features
CF®503R SE503/532 nm1 umol96078CF®503R Features
CF®514 SE516/548 nm1 umol92103CF®514 Features
CF®532 SE527/558 nm1 umol92104CF®532 Features
CF®543 SE541/560 nm1 umol92105CF®543 Features
CF®550R SE551/577 nm1 umol96073CF®550R Features
CF®555 SE555/565 nm1 umol92130CF®555 Features
CF®568 SE562/583 nm1 umol92131CF®568 Features
CF®570 SE568/591 nm1 umol96014CF®570 Features
CF®583 SE583/606 nm1 umol96016CF®583 Features
CF®583R SE586/609 nm1 umol96084CF®583R Features
CF®594 SE593/614 nm1 umol92132CF®594 Features
CF®597R SE597/619 nm1 umol96092CF®597R Features
CF®620R SE617/639 nm1 umol92106CF®620R Features
CF®633 SE630/650 nm1 umol92133CF®633 Features
CF®640R SE642/662 nm1 umol92108CF®640R Features
CF®647 SE650/665 nm1 umol92135CF®647 Features
CF®660R SE663/682 nm1 umol92134CF®660R Features
CF®660C SE667/685 nm1 umol92137CF®660C Features
CF®680 SE681/698 nm1 umol92139CF®680 Features
CF®680R SE680/701 nm1 umol92107CF®680R Features
CF®700 SE695/720 nm1 umol96067CF®700 Features
CF®750 SE755/779 nm1 umol92142CF®750 Features
CF®770 SE770/797 nm1 umol92150CF®770 Features
CF®790 SE784/806 nm0.25 umol92155CF®790 Features
CF®800 SE797/816 nm0.25 umol92127CF®800 Features
CF®820 SE822/835 nm0.25 umol96068CF®820 Features
CF®850 TFP852/870 nm0.25 umol96094CF®850 Features
CF®870 TFP876/896 nm0.25 umol96095CF®870 Features
Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific.

References

1. Biomater. Sci.(2019) 7, 1898-1904. DOI:10.1039/C8BM01378K
2. Biomaterials (2015) 67, 382-392. DOI:10.1016/j.biomaterials.2015.07.046
3. Microbiological Research (2018) 217, 69-80. DOI:10.1016/j.micres.2018.08.017

Download a list of CF® Dye references.

 

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