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CF® Dye SE/TFP Ester

CF® Dye SE/TFP esters are amine-reactive fluorescent dyes. They are commonly used to label primary amines of antibodies and other proteins, amine-modified oligonucleotides and other amine-containing molecules.

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Product Attributes

Chemical reactivity (reacts with)

Amines

Functional group

Tetrafluorophenyl Ester (TFP Ester), Succinimidyl Ester (NHS Ester)

Storage Conditions

Store at -10 to -35 °C, Protect from light

Conjugation
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0.25 umol
1 umol
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Product Description

CF® Dye SE/TFP Esters are amine-reactive forms of Biotium’s bright and photostable CF® Dyes. The succinimidyl ester (SE) group, also known as N-hydroxysuccinimide (NHS) esters, on the dye molecule reacts with primary amines such as lysine residues of antibodies or proteins, amine-modified oligonucleotides and other amine-containing molecules to form a stable amide linkage. CF® Dye TFP (tetrafluorophenyl) esters are more stable alternatives to succinimidyl ester. CF® Dye TFP esters are available for CF®850 and CF®870, Biotium’s industry-leading near-infrared dyes with emission above 850 nm.

  • Amine-reactive fluorescent dyes with high labeling efficiency
  • Label antibodies, proteins, amine-modified oligonucleotides and other amine-containing molecules.
  • Offered with Biotium’s bright and photostable CF® Dyes which provide superior signal-to-noise
  • Available in 36 CF® Dye colors from blue to near-IR wavelengths
  • Near-IR CF®850 and CF®870 TFP esters offer emission above 850 nm

Due to Biotium’s unique manufacturing and packaging process, our amine-reactive CF® Dyes are provided with the highest purity and more stable ester dye forms than other vendors. As a result, our CF® Dye SE/TFP products retain more reactivity and provide a higher labeling efficiency for users.

Superior CF® Dyes

CF® Dyes are Biotium’s line of next-generation fluorescent dyes that have improved brightness, photostability, and water solubility compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes. For more information download the CF® Dye Brochure.

Also see our CF® Dye SE Protein Labeling Kits and CF® Dye Mix-n-Stain™Antibody Labeling Kits.

CF® Dye SE/TFP Ester

Dye Ex/Em Size Catalog No. Dye Features
CF®350 SE 347/448 nm 1 umol 92109 CF®350 Features
CF®405S SE 404/431 nm 1 umol 92110 CF®405S Features
CF®405M SE 408/452 nm 1 umol 92111 CF®405M Features
CF®405L SE 395/545 nm 1 umol 92112 CF®405L Features
CF®430 SE 426/498 nm 1 umol 92117 CF®430 Features
CF®440 SE 440/515 nm 1 umol 92123 CF®440 Features
CF®450 SE 450/538 nm 1 umol 96011 CF®450 Features
CF®488A SE 490/515 nm 1 umol 92120 CF®488A Features
CF®503R SE 503/532 nm 1 umol 96078 CF®503R Features
CF®514 SE 516/548 nm 1 umol 92103 CF®514 Features
CF®532 SE 527/558 nm 1 umol 92104 CF®532 Features
CF®543 SE 541/560 nm 1 umol 92105 CF®543 Features
CF®550R SE 551/577 nm 1 umol 96073 CF®550R Features
CF®555 SE 555/565 nm 1 umol 92130 CF®555 Features
CF®568 SE 562/583 nm 1 umol 92131 CF®568 Features
CF®570 SE 568/591 nm 1 umol 96014 CF®570 Features
CF®583 SE 583/606 nm 1 umol 96016 CF®583 Features
CF®583R SE 586/609 nm 1 umol 96084 CF®583R Features
CF®594 SE 593/614 nm 1 umol 92132 CF®594 Features
CF®597R SE 597/619 nm 1 umol 96092 CF®597R Features
CF®620R SE 617/639 nm 1 umol 92106 CF®620R Features
CF®633 SE 630/650 nm 1 umol 92133 CF®633 Features
CF®640R SE 642/662 nm 1 umol 92108 CF®640R Features
CF®647 SE 650/665 nm 1 umol 92135 CF®647 Features
CF®660R SE 663/682 nm 1 umol 92134 CF®660R Features
CF®660C SE 667/685 nm 1 umol 92137 CF®660C Features
CF®680 SE 681/698 nm 1 umol 92139 CF®680 Features
CF®680R SE 680/701 nm 1 umol 92107 CF®680R Features
CF®700 SE 695/720 nm 1 umol 96067 CF®700 Features
CF®750 SE 755/779 nm 1 umol 92142 CF®750 Features
CF®770 SE 770/797 nm 1 umol 92150 CF®770 Features
CF®790 SE 784/806 nm 0.25 umol 92155 CF®790 Features
CF®800 SE 797/816 nm 0.25 umol 92127 CF®800 Features
CF®820 SE 822/835 nm 0.25 umol 96068 CF®820 Features
CF®850 TFP 852/870 nm 0.25 umol 96094 CF®850 Features
CF®870 TFP 876/896 nm 0.25 umol 96095 CF®870 Features
Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific.

References

1. Biomater. Sci.(2019) 7, 1898-1904. DOI:10.1039/C8BM01378K
2. Biomaterials (2015) 67, 382-392. DOI:10.1016/j.biomaterials.2015.07.046
3. Microbiological Research (2018) 217, 69-80. DOI:10.1016/j.micres.2018.08.017

Download a list of CF® Dye references.

 

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