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CF Dye Succinimidyl Ester

CF™ Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues.

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Catalog #

92137, 92106, 92107, 92108, 92109, 92110, 92111, 92112, 92117, 92123, 96011, 96014, 96016, 92105, 92104, 92139, 92142, 92150, 92127, 92130, 92131, 92132, 92133, 92134, 92135, 92155, 92103, - 92120View allHide

Size

0.25 umol, 1 umol

Conjugation

CF350, CF405L, CF405M, CF405S, CF430, CF440, CF450, CF488A, CF514, CF532, CF543, CF555, CF568, CF570, CF583, CF594, CF620R, CF633, CF640R, CF647, CF660C, CF660R, CF680, CF680R, CF750, CF770, CF790, CF800

Conjugation
Size
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0.25 umol
1 umol
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Taxes: Tax is assessed in the state of California and is calculated during checkout based on your shipping information. Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout.


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Product Description

CF™ Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues. CF™ dyes are Biotium’s line of next-generation fluorescent dyes, which have combined advantages in brightness, photostability, and signal-to-noise compared to other fluroescent dyes.

For more information, download the CF™ Dye Selection Guide.

Also see our CF™ Dye SE Protein Labeling Kits and CF™ Dye Mix-n-Stain™ Antibody Labeling Kits.

 

CF Dye Succinimidyl Ester: Product & SKU Summary

ConjugationProduct NameSizeSKUPurchase
CF350CF350 Succinimidyl Ester1 umol92109Purchase 92109
CF405LCF405L Succinimidyl Ester1 umol92112Purchase 92112
CF405MCF405M Succinimidyl Ester1 umol92111Purchase 92111
CF405SCF405S Succinimidyl Ester1 umol92110Purchase 92110
CF430CF430 Succinimidyl Ester1 umol92117Purchase 92117
CF440CF440 Succinimidyl Ester1 umol92123Purchase 92123
CF450CF450 Succinimidyl Ester1 umol96011Purchase 96011
CF488ACF488A Succinimidyl Ester1 umol92120Purchase 92120
CF514CF514 Succinimidyl Ester1 umol92103Purchase 92103
CF532CF532 Succinimidyl Ester1 umol92104Purchase 92104
CF543CF543 Succinimidyl Ester1 umol92105Purchase 92105
CF555CF555 Succinimidyl Ester1 umol92130Purchase 92130
CF568CF568 Succinimidyl Ester1 umol92131Purchase 92131
CF570CF570 Succinimidyl Ester1 umol96014Purchase 96014
CF583CF583 Succinimidyl Ester1 umol96016Purchase 96016
CF594CF594 Succinimidyl Ester1 umol92132Purchase 92132
CF620RCF620R Succinimidyl Ester1 umol92106Purchase 92106
CF633CF633 Succinimidyl Ester1 umol92133Purchase 92133
CF640RCF640R Succinimidyl Ester1 umol92108Purchase 92108
CF647CF647 Succinimidyl Ester1 umol92135Purchase 92135
CF660CCF660C Succinimidyl Ester1 umol92137Purchase 92137
CF660RCF660R Succinimidyl Ester1 umol92134Purchase 92134
CF680CF680 Succinimidyl Ester1 umol92139Purchase 92139
CF680RCF680R Succinimidyl Ester1 umol92107Purchase 92107
CF750CF750 Succinimidyl Ester1 umol92142Purchase 92142
CF770CF770 Succinimidyl Ester1 umol92150Purchase 92150
CF790CF790 Succinimidyl Ester0.25 umol92155Purchase 92155
CF800CF800 Succinimidyl Ester0.25 umol92127Purchase 92127

References

1. J Biol Chem 274, 21878 (1999).
2. J Biol Chem 273, 793 (1998).
3. Meth Mol Biol 80, 173 (1998).
4. Bioconjugate Chem 10, 32 (1999).
5. J Cell Biol 144, 99 (1999).
6. Anal Chem 70, 1242 (1998).
7. Meth Mol Biol 80, 173 (1998).

Download a list of CF dye references.

 

You may also like…

    Mix-n-Stain CF Dye Antibody Labeling Kits

    Mix-n-Stain™ antibody labeling kits are a revolutionary antibody labeling technology that allows you to label your antibody with biotin or one of our superior CF™ dyes in 30 minutes without a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. Now with an extended shelf life of at least 1 year.

    Cyanine Dye Succinimidyl Ester

    Biotium offers high quality amine-reactive succinimidyl ester (SE) dyes used to label proteins, oligonucleotides, and nucleic acids. We offer Cyanine 555 (Cy®3), Cyanine 647 (Cy®5), Cyanine 680 (Cy®5.5), and Cyanine 750 (Cy®7) as high quality succinimidyl esters.

    CF Dye Aminooxy

    Aminooxy dyes have an aminooxy reactive group, which can be used to label a target molecule with an aldehyde or ketone group. An aminooxy group readily reacts with an aldehyde or ketone group to form a stable linkage without the use of a reducing agent.

    CF Dye Amine

    CF™350 is spectrally similar to the traditional blue fluorescent dye, AMCA. However, compared to AMCA, CF™350 is more water-soluble, more photostable and at least 50% more fluorescent on proteins.

    DMSO, anhydrous

    Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.

    CF Dye Azide

    CF™ dye azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with a phosphine containing compound.

Support & Faq

Product shelf life, solubility, shipping and storage, and stability

Many of our solid compounds are packaged by lyophilization, in which case they usually do not appear as fluffy powders, but form a film or coating on the sides of the vial. Simply add the appropriate volume of the recommended solvent to the vial to make the desired concentration stock solution, and swirl or gently vortex to mix. Make sure the solvent comes in contact with the inside walls of the vial to fully recover the product.

Category: Product shelf life, solubility, shipping and storage, and stability

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Bioscience kits
The guaranteed shelf life from date of receipt for bioscience kits is listed on the product information sheet. Some kits have an expiration date printed on the kit box label, this is the guaranteed shelf life date calculated from the day that the product shipped from our facility. Kits often are functional for significantly longer than the guaranteed shelf life. If you have an older kit in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the kit still works for your application before processing a large number of samples or precious samples.

Antibodies and other conjugates
The guaranteed shelf life from date of receipt for antibodies and conjugates is listed on the product information sheet. Antibodies and other conjugates often are functional for significantly longer than the guaranteed shelf life. If you have an older conjugate in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples.

For lyophilized antibodies, we recommend reconstituting the antibody with glycerol and antimicrobial preservative like sodium azide for the longest shelf life (note that sodium azide is not compatible with HRP-conjugates).

Chemicals, dyes, and gel stains
Biotium guarantees the stability of chemicals, dyes, and gel stains for at least a year from the date you receive the product. However, the majority of these products are highly stable for many years, as long as they are stored as recommended. Storage conditions can be found on the product information sheet or product safety and data sheet, material safety data sheet, and on the product label. Fluorescent compounds should be protected from light for long term storage.

If you have a Biotium compound that has been in storage for longer than one year that you wish to use, we recommend performing a small scale positive control experiment to confirm that the compound still works for your application before processing a large number of samples or precious samples.

Expiration date based on date of manufacture (DOM)
If your institution requires you to document expiration date based on date of manufacture for reagents, please contact techsupport@biotium.com for assistance.

Chemical products with special stability considerations:

Esters

Ester compounds include the following:
• Succinimidyl esters (SE, also known as NHS esters), such as our amine-reactive dyes
• Acetoxymethyl esters (AM esters) such as our membrane-permeable ion indicator dyes
• Diacetate-modified dyes, like ViaFluor™ 405, CFDA, and CFDA-SE cell viability/cell proliferation dyes

Ester dyes are stable in solid form as long as they are protected from light and moisture. Esters are not stable in aqueous solution. Concentrated stock solutions should be prepared in anhydrous DMSO (see Biotium catalog no. 90082). Stock solutions in anhydrous DMSO can be stored desiccated at -20°C for one month or longer. Esters should be diluted in aqueous solution immediately before use. Succinimidyl esters (SE) should be dissolved in a solution that is free of amine-containing compounds like Tris, glycine, or protein, which will react with the SE functional group. AM esters and diacetate compounds should be dissolved in a solution that is free of serum, because serum could contain esterases that would hydrolyze the compound.

A note on CF™ dye succinimidyl ester stability
Succinimidyl esters are generally susceptible to hydrolysis, which can result in lower labeling efficiency. Heavily sulfonated dyes, such as the Alexa Fluor® dyes, DyLight® dyes and IRDyes® are particularly hygroscopic, worsening the hydrolysis problem. For example, the percent of active Alexa Fluor® 488 succinimidyl ester (SE) could be well below 50% by the time of application (according to the manufacturer’s product datasheet). In a number of Alexa Fluor® SE reactive dyes, the SE group is derived from an aromatic carboxylic acid, while in all of Biotium’s CF™ dyes the SE group is prepared from an aliphatic carboxylic acid. This structural difference reduces the susceptibility of CF™ dye SE reactive groups to hydrolysis, resulting in relatively stable reactive dyes with consistently higher labeling efficiency compared to other SE derivatives of other fluorescent dyes.

Maleimides, MTS and thiosulfate dyes
Like the succinimidyl ester dyes, these dyes are also susceptible to hydrolysis, although generally to a much lower degree. Thus, for long term storage, anhydrous DMSO is recommended for making stock solutions.

Other reactive dyes
Amines, aminooxy (also known as oxylamine), hydrazide, azide, alkyne, BCN, and tyramide reactive dyes, as well as dye free acids, are generally stable in aqueous solution when stored at -20°C for 6-12 months or longer, as long as no compounds are present that may react with the dye’s functional group. See the product information sheets for specific reactive dyes more information.

Coelenterazines and D-luciferin

Coelenterazines are stable in solid form when stored as recommended; they are not stable in aqueous solution. Concentrated coelenterazine stock solutions (typically 1-100 mg/mL) should be prepared in ethanol or methanol; do not use DMSO or DMF to dissolve coelenterazines, because these solvents will oxidize the compounds. Ethanol or methanol stocks of coelenterazine can be stored at -20°C or below for six months or longer; alcohol stocks may evaporate during storage, so use tightly sealing screw cap vials and wrap the vials with Parafilm for long term storage. Propylene glycol also can be used as a solvent to minimize evaporation. If the solvent evaporates, the coelenterazine will still be present in the vial, so note the volume in the vial prior to storage so that you can adjust the solvent volume to correct for evaporation if needed. Prepare working solutions in aqueous buffers immediately before use. Coelenterazines are stable for up to five hours in aqueous solution.

Aquaphile™ coelenterazines are water soluble formulations of coelenterazines. They are stable in solid form when stored as recommended. Aquaphile™ coelenterazines should be dissolved in aqueous solution immediately before use. They are stable for up to five hours in aqueous solution.

Note that coelenterazines are predominantly yellow solids, but may contain dark red or brown flecks. This does not affect product stability or performance. If your coelenterazine is uniformly brown, then it is oxidized and needs to be replaced.

D-luciferin is stable in solid form and as a concentrated stock solution when stored as recommended; it is not stable at dilute working concentrations in aqueous solution. Prepare concentrated D-luciferin stock solutions (typically 1-100 mg/mL) in water, and store in aliquots at -20°C or below for six months or longer. Prepare working solutions immediately before use.

Category: Mix-n-Stain™ Antibody Labeling Kits, DNA Quantitation Kits, NucView™ Caspase 3 Enzyme Substrates, Luciferase Assays, CF™ Dyes, Product shelf life, solubility, shipping and storage, and stability

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Most of our products are stable at room temperature for many days, but we recommend storage at 4°C or -20°C to prolong shelf life. In the case of many of our aqueous dye solutions, the compounds are very stable at room temperature, but we recommend cold storage to prevent the growth of mold or other microbes over time. Therefore, to save on shipping costs, products with recommended storage at 4°C or -20°C may ship at ambient temperature without affecting product performance. When you receive the product, place it under the recommended storage conditions.

Category: Mix-n-Stain™ Antibody Labeling Kits, DNA Quantitation Kits, NucView™ Caspase 3 Enzyme Substrates, Luciferase Assays, CF™ Dyes, Product shelf life, solubility, shipping and storage, and stability

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.