Fluorescein-5-maleimide is the most widely used green fluorescent thiol-reactive dye. The reaction requires very mild conditions and is highly specific.
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Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.
TCEP hydrochloride is an odorless reducing agent that is more stable and effective than dithiothreitol (DTT) or 2-Mercaptoethanol. TCEP hydrochloride is soluble in water to 310 grams per liter.
DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.
CF® Dye MTS
CF® dye MTS are fast-reacting, highly selective thiol-reactive derivatives for reversible labeling of thiol groups with fluorescent CF® dyes.